pBI-CMV3

价格:2200元

联系方式:I47-825O-882O

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载体基本信息

出品公司: Clontech
载体名称: pBI-CMV3
质粒类型: 哺乳细胞表达载体 ; 双向表达载体;荧光报告载体
高拷贝/低拷贝: 低拷贝
启动子: 弱启动子minCMV
克隆方法: 多克隆位点,限制性内切酶
载体大小: 3798 bp
5' 测序引物及序列: --
3' 测序引物及序列: --
载体标签: --
载体抗性: 氨苄青霉素(Ampicillin )
筛选标记: --
备注: pBI-CMV3表达亮度高的绿色荧光蛋白ZsGreen1
产品目录号: #631632
稳定性: 瞬表达
组成型/诱导型: 组成型
病毒/非病毒: 非病毒

载体质粒图谱和多克隆位点信息

pBI-CMV3载体图谱



pBI-CMV3多克隆位点

载体简介

双向启动子载体pBI-CMV3表达绿色荧光蛋白ZsGreen1
The pBI bidirectional vectors are designed to constitutively express a protein of interest and a reporter protein (or two proteins of interest). Expression is driven by constitutively active bidirectional human cytomegalovirus promoters (PminCMV). The gene(s) of interest must contain an initiation codon and a stop codon. 
eatures Constitutively express a protein of interest and a reporter protein (or two proteins of interest) 

载体序列

LOCUS       pBI-CMV3	3798 bp 	DNA	SYN
DEFINITION  pBI-CMV3
ACCESSION   
KEYWORDS    
SOURCE      
  ORGANISM  other sequences; artificial sequences; vectors.
FEATURES             Location/Qualifiers
     source          1..3798
                     /organism="pBI-CMV3"
                     /mol_type="other DNA"
     promoter        4..568
                     /label="CMV_immearly_promoter"
     misc_feature    71..358
                     /label="CAG_enhancer"
     misc_feature    525..545
                     /label="CMV_fwd_primer"
     promoter        526..595
                     /label="CMV_promoter"
     gene            complement(1799..2659)
                     /label="Ampicillin"
                     /gene="Ampicillin"
     CDS             complement(1799..2659)
                     /label="ORF frame 3"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
     promoter        complement(2701..2729)
                     /label="AmpR_promoter"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
     CDS             2969..16
                     /label="ORF frame 2"
                     /translation="MADYDPLDCSLRRSGPSGQGGAGGDGVLGQVPLLVLGVAAVLAG
                     QLVLDEVPVGHLAGHGLGLVHGVELAAQAATVLQQQVHAHVALQDALLGHGDDLLAGG
                     LPVVGHLLHHGAVGGEVHAVELGLVVHAVLLHAHGDVGVADAHGAVLEQEGAVPGVAG
                     GAGVLEVVDDVLGVLGEHAVAVHEGGGQDVLGEGQGAALHHAQVDGLLALEGVADALA
                     GDHELVAVHAALHAVLHGHLLGQAVLGLGHGGEFSRRSDGSLNELCLYRPPTVHATST
                     YSSSY*"
     CDS             complement(3000..3797)
                     /label="ORF frame 2"
                     /translation="MSRWRVRWEAYISRARLVNRQIAWRIRHHGPVQARPDQGDDHEV
                     PHGGLRGRPQVRDHRRGHRLPLQGQAGHQPVRGGGRPLALRRGHLVRRLHVRQPRVHR
                     VPPGHRRLLQELLPRRLHLGPLLPVRGRRRVHLQRRHHRERGGELHVPRVQVLRRELP
                     RRRPRDEEDDRQLGALLREDHPRAQAGHLEGRREHVPAAEGRWPLALPVRHRVQGQVR
                     APQDARLALHPAQADPRGPQRRQEPEVAPDRARHRLRLRLALRAQIS*"
     CDS             complement(3017..3712)
                     /label="ORF frame 3"
                     /translation="MAQSKHGLTKEMTMKYRMEGCVDGHKFVITGEGIGYPFKGKQAI
                     NLCVVEGGPLPFAEDILSAAFMYGNRVFTEYPQDIVDYFKNSCPAGYTWDRSFLFEDG
                     AVCICNADITVSVEENCMYHESKFYGVNFPADGPVMKKMTDNWEPSCEKIIPVPKQGI
                     LKGDVSMYLLLKDGGRLRCQFDTVYKAKSVPRKMPDWHFIQHKLTREDRSDAKNQKWH
                     LTEHAIASGSALP*"
     gene            complement(3020..3709)
                     /label="Zs_Green"
                     /gene="Zs_Green"
                     /translation="MAQSKHGLTKEMTMKYRMEGCVDGHKFVITGEGIGYPFKGKQAI
                     NLCVVEGGPLPFAEDILSAAFMYGNRVFTEYPQDIVDYFKNSCPAGYTWDRSFLFEDG
                     AVCICNADITVSVEENCMYHESKFYGVNFPADGPVMKKMTDNWEPSCEKIIPVPKQGI
                     LKGDVSMYLLLKDGGRLRCQFDTVYKAKSVPRKMPDWHFIQHKLTREDRSDAKNQKWH
                     LTEHAIASGSALP*"
     misc_feature    complement(3732..3756)
                     /label="LNCX_primer"
                     /translation="MAQSKHGLTKEMTMKYRMEGCVDGHKFVITGEGIGYPFKGKQAI
                     NLCVVEGGPLPFAEDILSAAFMYGNRVFTEYPQDIVDYFKNSCPAGYTWDRSFLFEDG
                     AVCICNADITVSVEENCMYHESKFYGVNFPADGPVMKKMTDNWEPSCEKIIPVPKQGI
                     LKGDVSMYLLLKDGGRLRCQFDTVYKAKSVPRKMPDWHFIQHKLTREDRSDAKNQKWH
                     LTEHAIASGSALP*"
     misc_feature    complement(3739..3758)
                     /label="pCEP_fwd_primer"
                     /translation="MAQSKHGLTKEMTMKYRMEGCVDGHKFVITGEGIGYPFKGKQAI
                     NLCVVEGGPLPFAEDILSAAFMYGNRVFTEYPQDIVDYFKNSCPAGYTWDRSFLFEDG
                     AVCICNADITVSVEENCMYHESKFYGVNFPADGPVMKKMTDNWEPSCEKIIPVPKQGI
                     LKGDVSMYLLLKDGGRLRCQFDTVYKAKSVPRKMPDWHFIQHKLTREDRSDAKNQKWH
                     LTEHAIASGSALP*"
ORIGIN
    1 ctcgagtagt tattaatagt aatcaattac ggggtcatta gttcatagcc catatatgga
   61 gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca acgacccccg
  121 cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga ctttccattg
  181 acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc aagtgtatca
  241 tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc
  301 ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat tagtcatcgc
  361 tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc
  421 acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa
  481 tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag
  541 gcgtgtacgg tgggaggtct atataagcag agctggttta gtgaaccgtc agatccgcta
  601 gggatcctct agtcagctga cgcgtgctag cgcggccgca tcgataagct tgtcgacgat
  661 atctccagag gatcataatc agccatacca catttgtaga ggttttactt gctttaaaaa
  721 acctcccaca cctccccctg aacctgaaac ataaaatgaa tgcaattgtt gttgttaact
  781 tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata
  841 aagcattttt ttcactgccc cgagcttcct cgctcactga ctcgctgcgc tcggtcgttc
  901 ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag
  961 gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa
 1021 aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc
 1081 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc
 1141 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg
 1201 cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt
 1261 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc
 1321 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc
 1381 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag
 1441 agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg
 1501 ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa
 1561 ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag
 1621 gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact
 1681 cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa
 1741 attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt
 1801 accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag
 1861 ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca
 1921 gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc
 1981 agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt
 2041 ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg
 2101 ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca
 2161 gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg
 2221 ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca
 2281 tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg
 2341 tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct
 2401 cttgcccggc gtcaacacgg gataataccg cgccacatag cagaacttta aaagtgctca
 2461 tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca
 2521 gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg
 2581 tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac
 2641 ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt
 2701 attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc
 2761 cgcgcacatt tccccgaaaa gtgccacctg acgtcggcag tgaaaaaaat gctttatttg
 2821 tgaaatttgt gatgctattg ctttatttgt aaccattata agctgcaata aacaagttaa
 2881 caacaacaat tgcattcatt ttatgtttca ggttcagggg gaggtgtggg aggtttttta
 2941 aagcaagtaa aacctctaca aatgtggtat ggctgattat gatcctctag actgcagcct
 3001 caggagatct gggccctcag ggcaaggcgg agccggaggc gatggcgtgc tcggtcaggt
 3061 gccacttctg gttcttggcg tcgctgcggt cctcgcgggt cagcttgtgc tggatgaagt
 3121 gccagtcggg catcttgcgg ggcacggact tggccttgta cacggtgtcg aactggcagc
 3181 gcaagcggcc accgtccttc agcagcaggt acatgctcac gtcgcccttc aagatgccct
 3241 gcttgggcac ggggatgatc ttctcgcagg agggctccca gttgtcggtc atcttcttca
 3301 tcacggggcc gtcggcgggg aagttcacgc cgtagaactt ggactcgtgg tacatgcagt
 3361 tctcctccac gctcacggtg atgtcggcgt tgcagatgca cacggcgccg tcctcgaaca
 3421 ggaaggagcg gtcccaggtg tagccggcgg ggcaggagtt cttgaagtag tcgacgatgt
 3481 cctgggggta ctcggtgaac acgcggttgc cgtacatgaa ggcggcggac aagatgtcct
 3541 cggcgaaggg caaggggccg ccctccacca cgcacaggtt gatggcctgc ttgcccttga
 3601 aggggtagcc gatgccctcg ccggtgatca cgaacttgtg gccgtccacg cagccctcca
 3661 tgcggtactt catggtcatc tccttggtca ggccgtgctt ggactgggcc atggtggcga
 3721 attctccagg cgatctgacg gttcactaaa cgagctctgc ttatataggc ctcccaccgt
 3781 acacgccacc tcgacata
//

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