pBABE-zeo

价格:1250元

联系方式:I47-825O-882O

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载体基本信息

出品人: Bob Weinberg
载体名称: pBABE-zeo
质粒类型: 逆病毒载体
高拷贝/低拷贝: 高拷贝
克隆方法: 限制性内切酶,多克隆位点
启动子: MSCV
载体大小: 4888bp
5' 测序引物及序列: pBABE 5' primer 5'CTTTATCCAGCCCTCAC3'
3' 测序引物及序列: pBABE 3' primer 5'ACCCTAACTGACACACATTCC3'
载体标签:
载体抗性: 氨苄青霉素
筛选标记: Zeocin
克隆菌株: DH5α、HB101等
宿主细胞(系): 常规哺乳动物细胞系
备注:
推荐使用逆病毒包装载体:pUMVCpCMV-VSV-G
 
产品目录号: #1766
稳定性: 稳表达
组成型/诱导型: 组成型
病毒/非病毒: 逆转录病毒

载体质粒图谱和多克隆位点信息

pBABE-zeo载体图谱



pBABE-zeo多克隆位点

载体简介

载体序列

LOCUS       pBABE-zeo (pBABE-bleo)	4888 bp 	DNA	SYN
DEFINITION  pBABE-zeo (pBABE-bleo)
ACCESSION   
KEYWORDS    
SOURCE      
  ORGANISM  other sequences; artificial sequences; vectors.
FEATURES             Location/Qualifiers
     source          1..4888
                     /organism="pBABE-zeo (pBABE-bleo)"
                     /mol_type="other DNA"
     misc_feature    12..479
                     /label="5_LTR2"
     misc_feature    18..479
                     /label="3MoMuLV_LTR"
     misc_feature    549..1350
                     /label="psi_plus_pack"
     misc_feature    1318..1334
                     /label="pBABE_5_primer"
     misc_feature    complement(1408..1428)
                     /label="pBABE_3_primer"
     misc_feature    complement(1414..1629)
                     /label="SV40_enhancer"
     promoter        1426..1694
                     /label="SV40_promoter"
     rep_origin      1593..1670
                     /label="SV40_origin"
     misc_feature    1655..1674
                     /label="SV40pro_F_primer"
     gene            1749..2120
                     /label="bleo"
                     /gene="bleo"
     misc_feature    1749..2123
                     /label="sh_ble"
     misc_feature    2169..2762
                     /label="3MoMuLV_LTR"
     misc_feature    2211..2762
                     /label="5_LTR2"
     gene            complement(3900..4760)
                     /label="Ampicillin"
                     /gene="Ampicillin"
     CDS             complement(3900..4760)
                     /label="ORF frame 3"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
     promoter        complement(4802..4830)
                     /label="AmpR_promoter"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
ORIGIN
    1 ctgcagcctg aatatgggcc aaacaggata tctgtggtaa gcagttcctg ccccggctca
   61 gggccaagaa cagatggaac agctgaatat gggccaaaca ggatatctgt ggtaagcagt
  121 tcctgccccg gctcagggcc aagaacagat ggtccccaga tgcggtccag ccctcagcag
  181 tttctagaga accatcagat gtttccaggg tgccccaagg acctgaaatg accctgtgcc
  241 ttatttgaac taaccaatca gttcgcttct cgcttctgtt cgcgcgcttc tgctccccga
  301 gctcaataaa agagcccaca acccctcact cggggcgcca gtcctccgat tgactgagtc
  361 gcccgggtac ccgtgtatcc aataaaccct cttgcagttg catccgactt gtggtctcgc
  421 tgttccttgg gagggtctcc tctgagtgat tgactacccg tcagcggggg tctttcattt
  481 gggggctcgt ccgggatcgg gagacccctg cccagggacc accgacccac caccgggagg
  541 taagctggcc agcaacttat ctgtgtctgt ccgattgtct agtgtctatg actgatttta
  601 tgcgcctgcg tcggtactag ttagctaact agctctgtat ctggcggacc cgtggtggaa
  661 ctgacgagtt ctgaacaccc ggccgcaacc ctgggagacg tcccagggac tttgggggcc
  721 gtttttgtgg cccgacctga ggaagggagt cgatgtggaa tccgaccccg tcaggatatg
  781 tggttctggt aggagacgag aacctaaaac agttcccgcc tccgtctgaa tttttgcttt
  841 cggtttggaa ccgaagccgc gcgtcttgtc tgctgcagca tcgttctgtg ttgtctctgt
  901 ctgactgtgt ttctgtattt gtctgaaaat tagggccaga ctgttaccac tcccttaagt
  961 ttgaccttag atcactggaa agatgtcgag cggctcgctc acaaccagtc ggtagatgtc
 1021 aagaagagac gttgggttac cttctgctct gcagaatggc caacctttaa cgtcggatgg
 1081 ccgcgagacg gcacctttaa ccgagacctc atcacccagg ttaagatcaa ggtcttttca
 1141 cctggcccgc atggacaccc agaccaggtc ccctacatcg tgacctggga agccttggct
 1201 tttgaccccc ctccctgggt caagcccttt gtacacccta agcctccgcc tcctcttctt
 1261 ccatccgcgc cgtctctccc ccttgaacct cctctttcga ccccgcctca atcctccctt
 1321 tatccagccc tcactccttc tctaggcgcc ggccggatcc cagtgtggtg gtacgtagga
 1381 attcgccagc acagtggtcg accctgtgga atgtgtgtca gttagggtgt ggaaagtccc
 1441 caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccaggt
 1501 gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt
 1561 cagcaaccat agtcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg
 1621 cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct
 1681 cggcctctga gctattccag aagtagtgag gaggcttttt tggaggccta ggcttttgca
 1741 aaaagcttat ggccaagttg accagtgccg ttccggtgct caccgcgcgc gacgtcgccg
 1801 gagcggtcga gttctggacc gaccggctcg ggttctcccg ggacttcgtg gaggacgact
 1861 tcgccggtgt ggtccgggac gacgtgaccc tgttcatcag cgcggtccag gaccaggtgg
 1921 tgccggacaa caccctggcc tgggtgtggg tgcgcggcct ggacgagctg tacgccgagt
 1981 ggtcggaggt cgtgtccacg aacttccggg acgcctccgg gccggccatg accgagatcg
 2041 gcgagcagcc gtgggggcgg gagttcgccc tgcgcgaccc ggccggcaac tgcgtgcact
 2101 tcgtggccga ggagcaggac tgaatcgata aaataaaaga ttttatttag tctccagaaa
 2161 aaggggggaa tgaaagaccc cacctgtagg tttggcaagc tagcttaagt aacgccattt
 2221 tgcaaggcat ggaaaaatac ataactgaga atagagaagt tcagatcaag gtcaggaaca
 2281 gatggaacag ctgaatatgg gccaaacagg atatctgtgg taagcagttc ctgccccggc
 2341 tcagggccaa gaacagatgg aacagctgaa tatgggccaa acaggatatc tgtggtaagc
 2401 agttcctgcc ccggctcagg gccaagaaca gatggtcccc agatgcggtc cagccctcag
 2461 cagtttctag agaaccatca gatgtttcca gggtgcccca aggacctgaa atgaccctgt
 2521 gccttatttg aactaaccaa tcagttcgct tctcgcttct gttcgcgcgc ttctgctccc
 2581 cgagctcaat aaaagagccc acaacccctc actcggggcg ccagtcctcc gattgactga
 2641 gtcgcccggg tacccgtgta tccaataaac cctcttgcag ttgcatccga cttgtggtct
 2701 cgctgttcct tgggagggtc tcctctgagt gattgactac ccgtcagcgg gggtctttca
 2761 tttccgactt gtggtctcgc tgccttggga gggtctcctc tgagtgattg actacccgtc
 2821 agcgggggtc ttcacatgca gcatgtatca aaattaattt ggtttttttt cttaagtatt
 2881 tacattaaat ggccatagtt gcattaatga atcggccaac gcgcggggag aggcggtttg
 2941 cgtattggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc
 3001 ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata
 3061 acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg
 3121 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct
 3181 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa
 3241 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc
 3301 tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt
 3361 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg
 3421 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg
 3481 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct
 3541 tgaagtggtg gcctaactac ggctacacta gaaggacagt atttggtatc tgcgctctgc
 3601 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg
 3661 ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc
 3721 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt
 3781 aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa
 3841 aatgaagttt gcggccgcaa atcaatctaa agtatatatg agtaaacttg gtctgacagt
 3901 taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata
 3961 gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc
 4021 agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac
 4081 cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag
 4141 tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac
 4201 gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc
 4261 agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg
 4321 gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc
 4381 atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct
 4441 gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc
 4501 tcttgcccgg cgtcaacacg ggataatacc gcgccacata gcagaacttt aaaagtgctc
 4561 atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc
 4621 agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc
 4681 gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca
 4741 cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt
 4801 tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt
 4861 ccgcgcacat ttccccgaaa agtgccac
//

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