| 出品人: | Bob Weinberg |
|---|---|
| 载体名称: | pBABE-zeo |
| 质粒类型: | 逆病毒载体 |
| 高拷贝/低拷贝: | 高拷贝 |
| 克隆方法: | 限制性内切酶，多克隆位点 |
| 启动子: | MSCV |
| 载体大小: | 4888bp |
| 5' 测序引物及序列: | pBABE 5' primer 5'CTTTATCCAGCCCTCAC3' |
| 3' 测序引物及序列: | pBABE 3' primer 5'ACCCTAACTGACACACATTCC3' |
| 载体标签: | 无 |
| 载体抗性: | 氨苄青霉素 |
| 筛选标记: | Zeocin |
| 克隆菌株: | DH5α、HB101等 |
| 宿主细胞（系）: | 常规哺乳动物细胞系 |
| 备注: |
推荐使用逆病毒包装载体：pUMVC、pCMV-VSV-G 。 |
| 产品目录号: | #1766 |
| 稳定性: | 稳表达 |
| 组成型/诱导型: | 组成型 |
| 病毒/非病毒: | 逆转录病毒 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pBABE-zeo (pBABE-bleo) 4888 bp DNA SYN
DEFINITION pBABE-zeo (pBABE-bleo)
ACCESSION
KEYWORDS
SOURCE
ORGANISM other sequences; artificial sequences; vectors.
FEATURES Location/Qualifiers
source 1..4888
/organism="pBABE-zeo (pBABE-bleo)"
/mol_type="other DNA"
misc_feature 12..479
/label="5_LTR2"
misc_feature 18..479
/label="3MoMuLV_LTR"
misc_feature 549..1350
/label="psi_plus_pack"
misc_feature 1318..1334
/label="pBABE_5_primer"
misc_feature complement(1408..1428)
/label="pBABE_3_primer"
misc_feature complement(1414..1629)
/label="SV40_enhancer"
promoter 1426..1694
/label="SV40_promoter"
rep_origin 1593..1670
/label="SV40_origin"
misc_feature 1655..1674
/label="SV40pro_F_primer"
gene 1749..2120
/label="bleo"
/gene="bleo"
misc_feature 1749..2123
/label="sh_ble"
misc_feature 2169..2762
/label="3MoMuLV_LTR"
misc_feature 2211..2762
/label="5_LTR2"
gene complement(3900..4760)
/label="Ampicillin"
/gene="Ampicillin"
CDS complement(3900..4760)
/label="ORF frame 3"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW*"
promoter complement(4802..4830)
/label="AmpR_promoter"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW*"
ORIGIN
1 ctgcagcctg aatatgggcc aaacaggata tctgtggtaa gcagttcctg ccccggctca
61 gggccaagaa cagatggaac agctgaatat gggccaaaca ggatatctgt ggtaagcagt
121 tcctgccccg gctcagggcc aagaacagat ggtccccaga tgcggtccag ccctcagcag
181 tttctagaga accatcagat gtttccaggg tgccccaagg acctgaaatg accctgtgcc
241 ttatttgaac taaccaatca gttcgcttct cgcttctgtt cgcgcgcttc tgctccccga
301 gctcaataaa agagcccaca acccctcact cggggcgcca gtcctccgat tgactgagtc
361 gcccgggtac ccgtgtatcc aataaaccct cttgcagttg catccgactt gtggtctcgc
421 tgttccttgg gagggtctcc tctgagtgat tgactacccg tcagcggggg tctttcattt
481 gggggctcgt ccgggatcgg gagacccctg cccagggacc accgacccac caccgggagg
541 taagctggcc agcaacttat ctgtgtctgt ccgattgtct agtgtctatg actgatttta
601 tgcgcctgcg tcggtactag ttagctaact agctctgtat ctggcggacc cgtggtggaa
661 ctgacgagtt ctgaacaccc ggccgcaacc ctgggagacg tcccagggac tttgggggcc
721 gtttttgtgg cccgacctga ggaagggagt cgatgtggaa tccgaccccg tcaggatatg
781 tggttctggt aggagacgag aacctaaaac agttcccgcc tccgtctgaa tttttgcttt
841 cggtttggaa ccgaagccgc gcgtcttgtc tgctgcagca tcgttctgtg ttgtctctgt
901 ctgactgtgt ttctgtattt gtctgaaaat tagggccaga ctgttaccac tcccttaagt
961 ttgaccttag atcactggaa agatgtcgag cggctcgctc acaaccagtc ggtagatgtc
1021 aagaagagac gttgggttac cttctgctct gcagaatggc caacctttaa cgtcggatgg
1081 ccgcgagacg gcacctttaa ccgagacctc atcacccagg ttaagatcaa ggtcttttca
1141 cctggcccgc atggacaccc agaccaggtc ccctacatcg tgacctggga agccttggct
1201 tttgaccccc ctccctgggt caagcccttt gtacacccta agcctccgcc tcctcttctt
1261 ccatccgcgc cgtctctccc ccttgaacct cctctttcga ccccgcctca atcctccctt
1321 tatccagccc tcactccttc tctaggcgcc ggccggatcc cagtgtggtg gtacgtagga
1381 attcgccagc acagtggtcg accctgtgga atgtgtgtca gttagggtgt ggaaagtccc
1441 caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccaggt
1501 gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt
1561 cagcaaccat agtcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg
1621 cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct
1681 cggcctctga gctattccag aagtagtgag gaggcttttt tggaggccta ggcttttgca
1741 aaaagcttat ggccaagttg accagtgccg ttccggtgct caccgcgcgc gacgtcgccg
1801 gagcggtcga gttctggacc gaccggctcg ggttctcccg ggacttcgtg gaggacgact
1861 tcgccggtgt ggtccgggac gacgtgaccc tgttcatcag cgcggtccag gaccaggtgg
1921 tgccggacaa caccctggcc tgggtgtggg tgcgcggcct ggacgagctg tacgccgagt
1981 ggtcggaggt cgtgtccacg aacttccggg acgcctccgg gccggccatg accgagatcg
2041 gcgagcagcc gtgggggcgg gagttcgccc tgcgcgaccc ggccggcaac tgcgtgcact
2101 tcgtggccga ggagcaggac tgaatcgata aaataaaaga ttttatttag tctccagaaa
2161 aaggggggaa tgaaagaccc cacctgtagg tttggcaagc tagcttaagt aacgccattt
2221 tgcaaggcat ggaaaaatac ataactgaga atagagaagt tcagatcaag gtcaggaaca
2281 gatggaacag ctgaatatgg gccaaacagg atatctgtgg taagcagttc ctgccccggc
2341 tcagggccaa gaacagatgg aacagctgaa tatgggccaa acaggatatc tgtggtaagc
2401 agttcctgcc ccggctcagg gccaagaaca gatggtcccc agatgcggtc cagccctcag
2461 cagtttctag agaaccatca gatgtttcca gggtgcccca aggacctgaa atgaccctgt
2521 gccttatttg aactaaccaa tcagttcgct tctcgcttct gttcgcgcgc ttctgctccc
2581 cgagctcaat aaaagagccc acaacccctc actcggggcg ccagtcctcc gattgactga
2641 gtcgcccggg tacccgtgta tccaataaac cctcttgcag ttgcatccga cttgtggtct
2701 cgctgttcct tgggagggtc tcctctgagt gattgactac ccgtcagcgg gggtctttca
2761 tttccgactt gtggtctcgc tgccttggga gggtctcctc tgagtgattg actacccgtc
2821 agcgggggtc ttcacatgca gcatgtatca aaattaattt ggtttttttt cttaagtatt
2881 tacattaaat ggccatagtt gcattaatga atcggccaac gcgcggggag aggcggtttg
2941 cgtattggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc
3001 ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata
3061 acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg
3121 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct
3181 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa
3241 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc
3301 tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt
3361 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg
3421 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg
3481 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct
3541 tgaagtggtg gcctaactac ggctacacta gaaggacagt atttggtatc tgcgctctgc
3601 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg
3661 ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc
3721 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt
3781 aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa
3841 aatgaagttt gcggccgcaa atcaatctaa agtatatatg agtaaacttg gtctgacagt
3901 taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata
3961 gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc
4021 agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac
4081 cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag
4141 tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac
4201 gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc
4261 agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg
4321 gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc
4381 atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct
4441 gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc
4501 tcttgcccgg cgtcaacacg ggataatacc gcgccacata gcagaacttt aaaagtgctc
4561 atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc
4621 agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc
4681 gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca
4741 cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt
4801 tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt
4861 ccgcgcacat ttccccgaaa agtgccac
//
