| 质粒名称: | pBbdCas9S |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 149638 |
| 存储实验室: | Christine Jacobs-Wagner |
|
载体骨架基本信息 |
|
| 载体名称: | pJSB252 |
| 载体出品公司: | J. Blevins |
| 载体抗性: | 大观霉素 |
| 空载体大小: | -- |
| 完整质粒大小: | -- |
| 载体修饰: | dCas9, lacI |
| 载体类型: | 细菌表达,CRISPR |
| 筛选标记: | -- |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 30 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | -- |
| 物种来源: | -- |
| 插入大小: | -- |
| 基因突变: | -- |
| GenBank编号 | -- |
| 启动子 | PflaB, PpQE30 |
| 标签/融合蛋白: | -- |
| 克隆方法: | Gibson克隆 |
| 5' 克隆位点: | -- |
| 3' 克隆位点: | -- |
| 5' 测序引物 | -- |
| 3' 测序引物 | -- |
|
文献引用方法 |
|
| 材料和方法部分: | pBbdCas9S was a gift from Christine Jacobs-Wagner (Addgene plasmid # 149638 ; http://n2t.net/addgene:149638 ; RRID:Addgene_149638) |
| 参考文献部分: | A CRISPR interference platform for selective downregulation of gene expression in Borrelia burgdorferi. Takacs CN, Scott M, Chang Y, Kloos ZA, Irnov I, Rosa PA, Liu J, Jacobs-Wagner C. Appl Environ Microbiol. 2020 Nov 30. pii: AEM.02519-20. doi: 10.1128/AEM.02519-20. 10.1128/AEM.02519-20 PubMed 33257311 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pBbdCas9S 10691 bp ds-DNA circular SYN 13-MAY-2021
DEFINITION parental, all-in-one CRISPRi vector for B. burgdorferi, empty.
ACCESSION .
VERSION .
KEYWORDS pBbdCas9S
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 10691)
AUTHORS Takacs CN, Scott M, Chang Y, Kloos ZA, Irnov I, Rosa PA, Liu J,
Jacobs-Wagner C
TITLE A CRISPR interference platform for selective downregulation of gene
expression in Borrelia burgdorferi.
JOURNAL Appl Environ Microbiol. 2020 Nov 30. pii: AEM.02519-20. doi:
10.1128/AEM.02519-20.
PUBMED 33257311
REFERENCE 2 (bases 1 to 10691)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..10691
/organism="synthetic DNA construct"
/mol_type="other DNA"
promoter 1746..1790
/label=T5 promoter
/note="bacteriophage T5 promoter for E. coli RNA
polymerase, with embedded lac operator"
protein_bind 1766..1782
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
protein_bind 1798..1814
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
RBS 1833..1844
/note="strong bacterial ribosome binding site (Elowitz and
Leibler, 2000)"
CDS 1851..5957
/codon_start=1
/product="catalytically dead mutant of the Cas9
endonuclease from the Streptococcus pyogenes Type II
CRISPR/Cas system"
/label=dCas9
/note="RNA-guided DNA-binding protein that lacks
endonuclease activity due to the D10A mutation in the RuvC
catalytic domain and the H840A mutation in the HNH
catalytic domain"
/translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
LYYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
TILERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
DATLIHQSITGLYETRIDLSQLGGD"
CDS 6127..6918
/codon_start=1
/gene="aadA"
/product="aminoglycoside adenylyltransferase (Murphy,
1985)"
/label=SmR
/note="confers resistance to spectinomycin and
streptomycin"
/translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
rep_origin 7041..7629
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 7530..7549
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
ORIGIN
1 agttctttta aaagagaatt tagtaaagcc ctaagtcttt taataaaaaa ttttttgtaa
61 aaaagttggc aaaaatagtt tttgctatat atttatatat aagaaaaaat tcaatactca
121 aggagataaa aaatggaaaa cttaaacaat aatccacaaa aaattaatca aataaataag
181 caaactaatt ttattggttt tgaaaatttt atagaacttg ataatcaagt taatcaaact
241 aaagaaaaaa gtaaattaag taagataggt aagaaattac ccggaataag cagtcaagtc
301 tagtgcggcc ggcgcgccaa ataatgcccg tgtccctgtt gaatagggac acggtttttt
361 aacaattttt ttattcctag gccttagtgg ccggtacccg gggatcctta ttactggccg
421 ctttctagcc tggatacttg tctggctagt tgcattagag agtcagctag agccctaggg
481 ctagctgttt gtgtattcgg agccagtgta gtttttcttt ttactaacga tacgggtaat
541 aattgattgc cttttacagc ctgaccttga gacaattgca aaagtctgtc cacagaggtt
601 tgacctaata atctaaaatc ttgtttgata gtagtaagtg gaggtatgta gcaactagaa
661 tcttctgtat catcataacc cacaactgaa atatcagccc caactctaag tccactttca
721 gtaatagctc gcatagctcc taaagccatt tgatcattag caactaacat agcagtaggg
781 acaatgcctt catttaacat ttgcattgtt tgctgaaatc ctgacatagc agaccaatct
841 ccttctcttt ctgctattgg ttgtatttga tttcttgtta aatacttgtg ccatcctgct
901 aatctcaatc tggcacttac tgaacttaag ggacctgcaa gtagtgcaat ttgttgatgg
961 cctaatgcaa caaggtgttc aactcctaac cttgttccat cttcgtgaga aaaaattata
1021 gagtttattg gggtttggtc cgacacatcc aaaaacaatg ctggaacatt agtacatgct
1081 gcttcaactg caatcgcatc ttggtcatca agaggataat taattataag tccactaaca
1141 cgttgagcta ataaattatg tactgctgcc ttacaagcct ctacacctga tctctcaacc
1201 attgatacaa caacgcttgc accaagttga tcagctctcg atttaattgc ggctacaatt
1261 tgactaggcg catgtaatgc aagtgatgat gtggcaactc caattaaaag tgattgtttc
1321 cccgcaagtt gctgagcaac gcgatttggt atataattca actctgccat agctgcttca
1381 accttctctc ttgtttttgc agatacatga cttgcttggt tcacaactct cgaaaccgtt
1441 tggtagctaa ccccggcata ttcagcaaca tcatacaaag taacaggttt catggatcat
1501 tcctccatga taaaatttaa atttctgact ttggcaaatc cttttgcctg ttaaaaagaa
1561 tagcacaaaa attaaaaaaa aagaaacgca aatagcaata gcattaaaaa taattattat
1621 tatttatttc tttgattaaa atcaaaagcc tttaatgtat tttcttgatc tttcatgcgc
1681 ttaacggtaa aatccaaggt attaagttca ccttccactt ttctctctct atcttctcta
1741 gaaaatcata aaaaatttat ttgctttgtg agcggataac aattataata gattcaattg
1801 tgagcggata acaatttcac acagaattca ttaaagagga gaaattacat atggataaga
1861 aatactcaat aggcttagct atcggcacaa atagcgtcgg atgggcggtg atcactgatg
1921 aatataaggt tccgtctaaa aagttcaagg ttctgggaaa tacagaccgc cacagtatca
1981 aaaaaaatct tataggggct cttttatttg acagtggaga gacagcggaa gcgactcgtc
2041 tcaaacggac agctcgtaga aggtatacac gtcggaagaa tcgtatttgt tatctacagg
2101 agattttttc aaatgagatg gcgaaagtag atgatagttt ctttcatcga cttgaagagt
2161 cttttttggt ggaagaagac aagaagcatg aacgtcatcc tatttttgga aatatagtag
2221 atgaagttgc ttatcatgag aaatatccaa ctatctatca tctgcgaaaa aaattggtag
2281 attctactga taaagcggat ttgcgcttaa tctatttggc cttagcgcat atgattaagt
2341 ttcgtggtca ttttttgatt gagggagatt taaatcctga taatagtgat gtggacaaac
2401 tatttatcca gttggtacaa acctacaatc aattatttga agaaaaccct attaacgcaa
2461 gtggagtaga tgctaaagcg attctttctg cacgattgag taaatcaaga cgattagaaa
2521 atctcattgc tcagctcccc ggtgagaaga aaaatggctt atttgggaat ctcattgctt
2581 tgtcattggg tttgacccct aattttaaat caaattttga tttggcagaa gatgctaaat
2641 tacagctttc aaaagatact tacgatgatg atttagataa tttattggcg caaattggag
2701 atcaatatgc tgatttgttt ttggcagcta agaatttatc agatgctatt ttactttcag
2761 atatcctaag agtaaatact gaaataacta aggctcccct atcagcttca atgattaaac
2821 gctacgatga acatcatcaa gacttgactc ttttaaaagc tttagttcga caacaacttc
2881 cagaaaagta taaagaaatc ttttttgatc aatcaaaaaa cggatatgca ggttatattg
2941 atgggggagc tagccaagaa gaattttata aatttatcaa accaatttta gaaaaaatgg
3001 atggtactga ggaattattg gtgaaactaa atcgtgaaga tttgctgcgc aagcaacgga
3061 cctttgacaa cggctctatt ccccatcaaa ttcacttggg tgagctgcat gctattttga
3121 gaagacaaga agacttttat ccatttttaa aagacaatcg tgagaagatt gaaaaaatct
3181 tgacttttcg aattccttat tatgttggtc cattggcgcg tggcaatagt cgttttgcat
3241 ggatgactcg gaagtctgaa gaaacaatta ccccatggaa ttttgaagaa gttgtcgata
3301 aaggtgcttc agctcaatca tttattgaac gcatgacaaa ctttgataaa aatcttccaa
3361 atgaaaaagt actaccaaaa catagtttgc tttatgagta ttttacggtt tataacgaat
3421 tgacaaaggt caaatatgtt actgaaggaa tgcgaaaacc agcatttctt tcaggtgaac
3481 agaagaaagc cattgttgat ttactcttca aaacaaatcg aaaagtaacc gttaagcaat
3541 taaaagaaga ttatttcaaa aaaatagaat gttttgatag tgttgaaatt tcaggagttg
3601 aagatagatt taatgcttca ttaggtacct accatgattt gctaaaaatt attaaagata
3661 aagatttttt ggataatgaa gaaaatgaag atatcttaga ggatattgtt ttaacattga
3721 ccttatttga agatagggag atgattgagg aaagacttaa aacatatgct cacctctttg
3781 atgataaggt gatgaaacag cttaaacgtc gccgttatac tggttgggga cgtttgtctc
3841 gaaaattgat taatggtatt agggataagc aatctggcaa aacaatatta gattttttga
3901 aatcagatgg ttttgccaat cgcaatttta tgcagctgat ccatgatgat agtttgacat
3961 ttaaagaaga cattcaaaaa gcacaagtgt ctggacaagg cgatagttta catgaacata
4021 ttgcaaattt agctggtagc cctgctatta aaaaaggtat tttacagact gtaaaagttg
4081 ttgatgaatt ggtcaaagta atggggcggc ataagccaga aaatatcgtt attgaaatgg
4141 cacgtgaaaa tcagacaact caaaagggcc agaaaaattc gcgagagcgt atgaaacgaa
4201 tcgaagaagg tatcaaagaa ttaggaagtc agattcttaa agagcatcct gttgaaaata
4261 ctcaattgca aaatgaaaag ctctatctct attatctcca aaatggaaga gacatgtatg
4321 tggaccaaga attagatatt aatcgtttaa gtgattatga tgtcgatgcc attgttccac
4381 aaagtttcct taaagacgat tcaatagaca ataaggtctt aacgcgttct gataaaaatc
4441 gtggtaaatc ggataacgtt ccaagtgaag aagtagtcaa aaagatgaaa aactattgga
4501 gacaacttct aaacgccaag ttaatcactc aacgtaagtt tgataattta acgaaagctg
4561 aacgtggagg tttgagtgaa cttgataaag ctggttttat caaacgccaa ttggttgaaa
4621 ctcgccaaat cactaagcat gtggcacaaa ttttggatag tcgcatgaat actaaatacg
4681 atgaaaatga taaacttatt cgagaggtta aagtgattac cttaaaatct aaattagttt
4741 ctgacttccg aaaagatttc caattctata aagtacgtga gattaacaat taccatcatg
4801 cccatgatgc gtatctaaat gccgtcgttg gaactgcttt gattaagaaa tatccaaaac
4861 ttgaatcgga gtttgtctat ggtgattata aagtttatga tgttcgtaaa atgattgcta
4921 agtctgagca agaaataggc aaagcaaccg caaaatattt cttttactct aatatcatga
4981 acttcttcaa aacagaaatt acacttgcaa atggagagat tcgcaaacgc cctctaatcg
5041 aaactaatgg ggaaactgga gaaattgtct gggataaagg gcgagatttt gccacagtgc
5101 gcaaagtatt gtccatgccc caagtcaata ttgtcaagaa aacagaagtc cagacaggcg
5161 gattctccaa ggagtcaatt ttaccaaaaa gaaattcgga caagcttatt gctcgtaaaa
5221 aagactggga tccaaaaaaa tatggtggtt ttgatagtcc aacggtagct tattcagtcc
5281 tagtggttgc taaggtggaa aaagggaaat cgaagaagtt aaaatccgtt aaagagttac
5341 tagggatcac aattctggaa agaagttcct ttgaaaaaaa tccgattgac tttttagaag
5401 ctaaaggata taaggaagtt aaaaaagact taatcattaa actacctaaa tatagtcttt
5461 ttgagttaga aaacggtcgt aaacggatgc tggctagtgc cggagaatta caaaaaggaa
5521 atgagctggc tctgccaagc aaatatgtga attttttata tttagctagt cattatgaaa
5581 agttgaaggg tagtccagaa gataacgaac aaaaacaatt gtttgtggag cagcataagc
5641 attatttaga tgagattatt gagcaaatca gtgaattttc taagcgtgtt attttagcag
5701 atgccaattt agataaagtt cttagtgcat ataacaaaca tagagacaaa ccaatacgtg
5761 aacaagcaga aaatattatt catttattta cgttgacgaa tcttggagct cccgctgctt
5821 ttaaatattt tgatacaaca attgatcgta aacgatatac gtctacaaaa gaagttttag
5881 atgccactct tatccatcaa tccatcactg gtctttatga aacacgcatt gatttgagtc
5941 agctaggagg tgactaataa aagcttgatc agatctcagc ttttttttga agtgcctggc
6001 agtaagttga aaaaatttga aataaacttg gactatgtta ataaaaaaat acaaattaac
6061 gaacaaaaag atttgaatta ttatataatt tagttaaaag caattttaaa tgagggaggt
6121 ttccatatga gggaagcggt gatcgccgaa gtatcgactc aactatcaga ggtagttggc
6181 gtcatcgagc gccatctcga accgacgttg ctggccgtac atttgtacgg ctccgcagtg
6241 gatggcggcc tgaagccaca cagtgatatt gatttgctgg ttacggtgac cgtaaggctt
6301 gatgaaacaa cgcggcgagc tttgatcaac gaccttttgg aaacttcggc ttcccctgga
6361 gagagcgaga ttctccgcgc tgtagaagtc accattgttg tgcacgacga catcattccg
6421 tggcgttatc cagctaagcg cgaactgcaa tttggagaat ggcagcgcaa tgacattctt
6481 gcaggtatct tcgagccagc cacgatcgac attgatctgg ctatcttgct gacaaaagca
6541 agagaacata gcgttgcctt ggtaggtcca gcggcggagg aactctttga tccggttcct
6601 gaacaggatc tatttgaggc gctaaatgaa accttaacgc tatggaactc gccgcccgac
6661 tgggctggcg atgagcgaaa tgtagtgctt acgttgtccc gcatttggta cagcgcagta
6721 accggcaaaa tcgcgccgaa ggatgtcgct gccgactggg caatggagcg cctgccggcc
6781 cagtatcagc ccgtcatact tgaagctaga caggcttatc ttggacaaga agaagatcgc
6841 ttggcctcgc gcgcagatca gttggaagaa tttgtccact acgtgaaagg cgagatcacc
6901 aaggtagtcg gcaaataatg acgtcgccgg agcggtcgag ttctggaccg accggctcgg
6961 gttctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta
7021 gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa
7081 acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt
7141 tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag
7201 ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta
7261 atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca
7321 agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag
7381 cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa
7441 agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga
7501 acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc
7561 gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc
7621 ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt
7681 gctcacatgt tctttcctgc actagatccc cggcaagttc atccttaggg ttttcatatc
7741 tcttaaaaag tcaatttctg cttgattaat ttcttgtgga ttattgtttt tgcggttttt
7801 cattttttat ctccttgagt attgaatttt ttcttatata taaatatata gcaaaaacta
7861 tttttgccaa cttttttaca aaaaattttt tattaaaaca cttagggctt tgctaaattc
7921 tcttttaaaa gaacttagta aagccctatg gatttaagaa ctgattatac ttacgtagta
7981 aaaatactac agatattaat agtatagacc atatcaaaat cataatcaat ttatatttat
8041 ctaaaattgt agtccataca aaatacaaaa aaattaggtt tttctaaatt ctattttaaa
8101 agaacttagt aacacctaat ctctaaatta cttacaaaat tctaaaatta gatttttagt
8161 tcttcatatt tctttaaaag ttttttaaga aaatcttttt gattttcaaa aatttcatgc
8221 atcataaaac tagtaaattt ggattttttt ttgtaaaaat cataactttc ttgggtttta
8281 agttgaaatc ttaatggttt tattggattt tgcctagatt tttttaacgc cggactttct
8341 ttatctttta taaaattata agaattttta ataccatttt ctattaaata atcttctttt
8401 ataagcccct cttgcagtgc agttgctaat cttaaatact tatacgcttg agtttttgct
8461 acatcaaagt cttttataaa atcaagaaaa gttttataac catcaaattt ataatattga
8521 ttatctttta tttcttttaa aattttaata ttttccaccc tgtggaatat ttcttttttg
8581 aaattgtcat ttaacttttc ctttagtgtc ttgtaataag aaattttact ttcattaatc
8641 attaaatttt gttcaacata agtatctatc ttttttgtta aaactttaac aacacgtttt
8701 ttttccataa aaattttcct ttatttttgc aacattttcc ttaattgata taattgttac
8761 tattttagat tttttaatat ccacttaaga attaatccct cactagaata ttttttatta
8821 tagaattcat atacatgttt ttctaaatct ataaatttat caacaattgc tttattatag
8881 ggtgtatcta tcttttcttt tcttaataat cgtctcatat tctttaaata acaacaaaca
8941 ctgccggttt taaacctaaa ttctatgtaa tatatttttg aaaaagtata tacattttca
9001 accccgttta tttcatactt tacaaaaaca ttatttattg gttttctata accatagtaa
9061 atgcctaaaa atttgtcccc gtctttcata ggatatagat aaagtttttg aatctctgat
9121 ttatctaata atttttcaaa aaaaattata caccgctttt gtttgttact aactactcta
9181 tgcaatgcct ttaatatttt tgtgtgatat atatttttat cacctttttt ttctttcagc
9241 aggaagatac tttcttttgg aaatttaatt ttttgttcat ttattcttgc agccttaagt
9301 ccgattttca tatttaatcc ttatatagcc tttctattta attcttttaa actcaataaa
9361 cttttttggt ttttcattag ttctaataat tcgcaataat atgtgcaaaa cactttattg
9421 tattctattt tcttttggtt attcaaatat tcttttataa ttggctttag aagttcaata
9481 tttacttctt tttttagttg ttcaataagg atattgaaaa tgtttgtttt taaatcttga
9541 taattttgtt gagaattttc tttttttctt tcaattgact tctctaattt acgctttata
9601 ttgtttaaat cgctatattt atgactttca ataataaaat gaggcttaaa tttgtaattt
9661 tcgtatattt tttggaaact tgtttctaat tgtttagaat tatattcatt tttttcaaat
9721 tctttttgag tgttgcatag aatttccttt aatttttctt gtttttcttt catacaagat
9781 ttagtaaaat gtatattttt attttttagt aatttaattt ctattatttt tatgatttta
9841 agtattttga ttatattatc tttatcaata tttaaattga ataaaattgt aagaaaaatt
9901 gaaagaattt ttttacataa aaagttacat ttattgaagt aatttcttac ttgatacttt
9961 tctatttcat taatctttct ttcttctttt atattatttt tattatttaa acactccttt
10021 aaatctacac tcccattttt agtaaatcta tctttaaggc cccttgaaac tctattttga
10081 aatcttaaat cttttttttc ttgaaagtat ttatagattt tttggtaaca ttcttttttt
10141 gaaaaattaa gttgatagta tatttcggtc cccaaattta cccctaagtg tttgtgataa
10201 ttagttgtga ctttaaattc tttttctaat ttgtaaaggt aattttgcag tgtttttagt
10261 ttaacgggtg tttgactatt tcttttaagg ttttcattaa agtaatagag tatgtttttt
10321 tgtgtgtatt tcttatatgt tttatttatg tacgctagtg ttgagataag gactactaat
10381 ttatactgta atttattgta gcaagttgtg gttttactgg ttttgtttat attagataag
10441 ttttccattt atagctcctt gaataccgga ttttttatct ataaagtata tatatcacaa
10501 ttttgattta aaagtaaatt ctttttttaa aaatacaaaa attttttata aattatatag
10561 accatgatat tgtattaata ctaattgatt ttaatttaag gttgaactaa aataaacata
10621 gttttgtaga aaataatttt tcattatttt gctacttgat attggatcgt aaaaatatta
10681 gggctttaca a
//
