pHT315

LOCUS       pHT315                  6777 bp DNA     circular SYN 14-JUN-2024
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6777)
  AUTHORS   Ming
  TITLE     Direct Submission
  JOURNAL   Exported Jun 14, 2024 from SnapGene 6.0.2 to Vector NTI(R) format
            https://www.biofeng.com
COMMENT     VNTDATE|1060128000|
COMMENT     VNTDBDATE|1060128000|
COMMENT     VNTNAME|pHT315|
COMMENT     VNTAUTHORNAME|Ming|
FEATURES             Location/Qualifiers
     CDS             complement(137..515)
                     /label=ISS
                     /note="immunostimulatory sequence from the AmpR gene; 
                     contains unmethylated CpG dinucleotides in the context of 
                     5'-AACGTT-3' (Sato et al., 1996)"
     CDS             complement(4889..5212)
                     /codon_start=1
                     /gene="lacZ fragment"
                     /product="LacZ-alpha fragment of beta-galactosidase"
                     /label=lacZ-alpha
     primer_bind     5122..5138
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    5139..5195
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(5208..5224)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    5232..5248
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(5256..5286)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5301..5322
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(5610..6198)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 cagatttatc agcataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac
       61 tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc
      121 agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc
      181 gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc
      241 catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt
      301 ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc
      361 atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg
      421 tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag
      481 cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat
      541 cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc
      601 atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa
      661 aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatgtac
      721 cccaaattcc ccgtaggcgc tagggacctc tttagcttct tggaagctgt cagtagtata
      781 tctaataatt tatctccatt ccctttagta acgtgtaact ttccaaattt aaaaaagcga
      841 ctcatagaat tatttcctcc cgttaaataa tagataacta ttaaaaatag acaatacttg
      901 ctcataagta atggtactta aattgtttac tttggcgtgt ttcattgctt gatgaaactg
      961 atttttagta aacagttgac gatattctcg attgacccat tttgaaacaa agtacgtata
     1021 tagcttccaa tatttatctg gaacatctgt ggtatggcgg gtaagtttta ttaagacact
     1081 gtttactttt ggtttaggat gaaagcattc cgctggcagc ttaagcaatt gctgaatcga
     1141 gacttgagtg tgcaagagca accctagtgt tcggtgaata tccaaggtac gcttgtagaa
     1201 tccttcttca acaatcagat agatgtcaga cgcatggctt tcaaaaacca cttttttaat
     1261 aatttgtgtg cttaaatggt aaggaatact cccaacaatt ttatacctct gtttgttagg
     1321 gaattgaaac tgtagaatat cttggtgaat taaagtgaca cgaatgttca gttttaattt
     1381 ttctgacgat aagttgaata gatgactgtc taattcaata gacgttacct gtttacttat
     1441 tttagccagt ttcgtcgtta aatgcccttt acctgttcca atttcgtaaa cggtatcggt
     1501 ttcttttaaa ttcaattgtt ttattatttg gttgagtact ttttcactcg ttaaaaagtt
     1561 ttgagaatat tttatatttt tgttcatgta attactcctg aagtgattac atctgtaaat
     1621 aaatacagaa gttaaacgat ttgtttgtaa ttttagttat ctgtttaaaa agtcataaga
     1681 ttagtcactg gtaggaatta atctaacgta tttatttatc tgcgtaatca ctgtttttag
     1741 tctgtttcaa aacagtagat gttttatcta cattacgcat ttggaatacc aacatgacga
     1801 atccctcctt cttaattaca aatttttagc atctaattta acttcaattc ctattataca
     1861 caaaatttta agatactgca ctatcaacac actcttaagt ttgcttcggg gatcaacata
     1921 ttagatatcg tggtgttcaa aaacgctacc tcatgatcct gcaatgatat aactttatct
     1981 tgcaaatcct ttacgacatc agtagatggc gccgctgtaa atagtatccc cagcatcaat
     2041 agttccccct gattatataa attcagacga atactattat ccattatttt tttattaagg
     2101 taaattaata ttttacctat taaaggaggg acgatttaag tatggacgtg atgtttggtc
     2161 aaggtgggaa cataaatttt aattacattg gaacatattg tggacaagca tctaattcat
     2221 aatccccact ttccaagcat gaaagtccct tataccatca attattcaat aaaatatccc
     2281 tatcataaac ctgtcccgcg cgacggttca aatactcctt tatgataaaa taaagatata
     2341 aagagacgag atgagggaga aatttgacgg ctaataaaaa aaagattgta aataaaccaa
     2401 ctgttgtggc aaaacttcca caaatcatta tagatacata taacttgact tgcacttcta
     2461 acgatgtaaa aatgtttccc ggatttataa agcacgtaaa gaaacaacat cctggtattt
     2521 atgaaaaata ttcatcgcac ataaaagata tcgttgaaca ccccgactat gtcgggcaaa
     2581 atcctaaaga acctaatagc gttgaattgg taaagatttt aaatgaccat atactaattg
     2641 ctataaagtt ggacccaagc ggttatttgt tcttatcaac tatgttcgat ctaaaaaacg
     2701 gtcctgcgaa aatccaaaga cgattgaata gtggacgttt aattgcttac aaagacctgt
     2761 taagctaacg gggagtttac taaaatataa gaacatggta tactacctgt agaaagtaac
     2821 ataataatga tataggatct ttgaggtggg aaaggttccc cacgcgctta tctaaccgcc
     2881 gttctcaagc atttgattgg ctaggtaagt taccagagat gtaggatacg ccgccctacc
     2941 aacgatccta tgtacggtaa gccatccgtt ttttacggat ggctttttat attgactttt
     3001 tcatcgctaa tggttacttt tctatcaaac gacaccgaaa ggcgatgatt attgtgttaa
     3061 aaaacccaat attataacat ccactaatgg ataatcttac ttttagtaag taggttgcct
     3121 agtgaaatac aataattcca acattgggcc ttaatacata ttaattagtt ttaaccctct
     3181 cactataaaa gagttaaata tgactccgca acctctttaa tatcactatt acattttgta
     3241 acaacattat tactttcatc ctctgtacca ttaacaaata aatgaggatt ctttaccata
     3301 aagtctaaca atagatcaat aaaccgctgc ctttgttctc tagccatttc ctttctcata
     3361 gagaaccact cccctatctt tcactattct tttaatttca tcttgtcttt cacttgattt
     3421 aaacagctct ggattcttcc tcaatccccc actatcttag ttaatggtag ttgcttcagt
     3481 actctacatt ttttgctaat cgaggttaaa atccttcaaa tcccttgact ttaaggtctt
     3541 aaggttttca tactggcgag tttgttagga aatctgaatg tagttttggg catagtaacc
     3601 cctgttgaaa atttacgagg ttaaaacgcc cactcattcg tactggctta gatgccgagg
     3661 tctcgaattg agattggttg cattgacgac aaacggttat agcttacaaa actactatac
     3721 cgtccgtata tagcttattg gcattgtatt gcgccgtacg gtgcgtttct cacgcccaac
     3781 accgttccct ttcgggtgat tcgctatata acccgcatat ccttgtaata aagctagctt
     3841 gtacaagaat agccgtgaat cagtgtcatg atcgccgctt acgagtaact gtttaactcc
     3901 agtcgcacaa tcatgtctta ttttgaaccc ccaacacttt ttgccgctaa cgtcttggtt
     3961 atcatcatta ttaggattta catcccgtag gcttgtttta tgcaaggctt gtgtccttta
     4021 cctcgcttac gtcagacagt tgaatgcaac gacaactgat atccgagtgt actctcctgc
     4081 attggctaca ctagaccatc tttttaaagt ggtagcttcc actggaacgg aacacaaaaa
     4141 gggcgttctc tatatctaaa cgccctgtac atttacaaga cttctaggta tagaaaacgc
     4201 ccttttatat ccgttttatt atgtatatga caatgctagt aattactagt tgaaatattc
     4261 gtagagtaac ggtacaatag gtgtatctaa taagccttgt tcgcgaaaac aaggcaaata
     4321 acttatttga agttggcgcc tcttataagt cgaagtcctg tatggctgta caggattaag
     4381 tcattctcgc taaaggttgg tagccaatag catatgagag tggctttttt catttccgtt
     4441 ctattaagtt actgctaatt ttaccgctat gtttctatta aatcaacgaa aatttgataa
     4501 ggttcaccag aaacattaat atttgcgaca taaaactcac tctccaactt tggaggatgg
     4561 attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt
     4621 agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgtct
     4681 aagaaaccat tattatcatg acattaacct ataaaaatag gcgtatcacg aggccctttc
     4741 gtctcgcgcg tttcggtgat gacggtgaaa acctctgaca catgcagctc ccggagacgg
     4801 tcacagcttg tctgtaagcg gatgccggga gcagacaagc ccgtcagggc gcgtcagcgg
     4861 gtgttggcgg gtgtcggggc tggcttaact atgcggcatc agagcagatt gtactgagag
     4921 tgcaccatat gcggtgtgaa ataccgcaca gatgcgtaag gagaaaatac cgcatcaggc
     4981 gccattcgcc attcaggctg cgcaactgtt gggaagggcg atcggtgcgg gcctcttcgc
     5041 tattacgcca gctggcgaaa gggggatgtg ctgcaaggcg attaagttgg gtaacgccag
     5101 ggttttccca gtcacgacgt tgtaaaacga cggccagtga attcgagctc ggtacccggg
     5161 gatcctctag agtcgacctg caggcatgca agcttggcgt aatcatggtc atagctgttt
     5221 cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag
     5281 tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg
     5341 cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg
     5401 gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc
     5461 tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc
     5521 acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg
     5581 aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat
     5641 cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag
     5701 gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga
     5761 tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg
     5821 tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt
     5881 cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac
     5941 gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc
     6001 ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt
     6061 ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc
     6121 ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc
     6181 agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg
     6241 aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag
     6301 atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg
     6361 tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt
     6421 tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca
     6481 tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca
     6541 gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc
     6601 tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt
     6661 ttgcgcaacg ttgttgccat tgctacaggc atcgtgtgtc acgctcgtcg tttggtatgg
     6721 cttcattcag ctcggtccca cgatcagcga gtacatgatc ccccatgtgt gcaaaaa
//