| 质粒名称: | pTns(Ptr) |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 175576 |
| 存储实验室: | Sheng Yang |
|
载体骨架基本信息 |
|
| 载体名称: | pCOLADuet-1 |
| 完整质粒大小: | 6342 bp |
| 载体类型: | 细菌表达 |
| 载体抗性: | Kanamycin |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5a |
| 拷贝数: | 高拷贝 |
|
插入基因信息 |
|
| 插入基因名称: | PtrTnsABC |
| 物种来源: | Pseudoalteromonas translucida |
| 基因突变: | No |
| 克隆方法: | Gibson Cloning |
|
文献引用方法 |
|
| 材料和方法部分: |
pTns(Ptr) was a gift from Sheng Yang (Addgene plasmid # 175576 ; http://n2t.net/addgene:175576 ; RRID:Addgene_175576)
|
| 参考文献部分: | Orthogonal CRISPR-associated transposases for parallel and multiplexed chromosomal integration. Yang S, Zhang Y, Xu J, Zhang J, Zhang J, Yang J, Jiang Y, Yang S. Nucleic Acids Res. 2021 Sep 3. pii: 6363765. doi: 10.1093/nar/gkab752. 10.1093/nar/gkab752 PubMed 34478496 |
pTns(Ptr)
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
买家导航
pTns(Ptr)载体质粒基本信息
pTns(Ptr)质粒图谱载体图谱和pTns(Ptr)载体序列质粒序列多克隆位点信息
质粒图谱.png)
pTns(Ptr)质粒载体简介
pTns(Ptr)质粒序列载体序列
LOCUS pTns(Ptr) 6342 bp ds-DNA circular SYN 03-NOV-2021
DEFINITION Expression of CRISPR-associated transposases.
ACCESSION .
VERSION .
KEYWORDS pTns(Ptr)
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6342)
AUTHORS Yang S, Zhang Y, Xu J, Zhang J, Zhang J, Yang J, Jiang Y, Yang S
TITLE Orthogonal CRISPR-associated transposases for parallel and
multiplexed chromosomal integration.
JOURNAL Nucleic Acids Res. 2021 Sep 3. pii: 6363765. doi:
10.1093/nar/gkab752.
PUBMED 34478496
REFERENCE 2 (bases 1 to 6342)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Nov 3, 2021 from SnapGene Server 1.1.58
http://www.biofeng.com
FEATURES Location/Qualifiers
source 1..6342
/organism="synthetic DNA construct"
/mol_type="other DNA"
primer_bind complement(3308..3326)
/label=T7 Term
/note="T7 terminator, reverse primer"
terminator 3322..3369
/label=T7 terminator
/note="transcription terminator for bacteriophage T7 RNA
polymerase"
CDS complement(3599..4414)
/codon_start=1
/gene="aph(3')-Ia"
/product="aminoglycoside phosphotransferase"
/label=KanR
/note="confers resistance to kanamycin in bacteria or G418
(Geneticin(R)) in eukaryotes"
/translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
primer_bind 4322..4341
/label=Kan-R
/note="Kanamycin resistance gene, reverse primer"
promoter complement(4415..4506)
/gene="bla"
/label=AmpR promoter
rep_origin complement(4524..5159)
/direction=LEFT
/label=ColA ori
/note="Plasmids containing the ColA origin of replication
can be propagated in E. coli cells that contain additional
plasmids with compatible origins."
primer_bind complement(4669..4688)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(5419..6042)
/codon_start=1
/gene="tetR from transposon Tn10"
/product="tetracycline repressor TetR"
/label=TetR
/note="TetR binds to the tetracycline operator tetO to
inhibit transcription. This inhibition can be relieved by
adding tetracycline or doxycycline."
/translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHV
KNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTT
DSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
primer_bind 5950..5969
/label=Tet-R
/note="Tetracycline resistance gene, reverse primer"
promoter 6061..6116
/gene="tetR"
/label=tetR/tetA promoters
/note="overlapping promoters for bacterial tetR and tetA"
protein_bind 6067..6085
/label=tet operator
/bound_moiety="tetracycline repressor TetR"
/note="bacterial operator O1 for the tetR and tetA genes"
protein_bind 6097..6115
/gene="tetO"
/label=tet operator
/bound_moiety="tetracycline repressor TetR"
/note="bacterial operator O2 for the tetR and tetA genes"
ORIGIN
1 agcgcaaccg ctgggttacg agtatgaatt cgataaccgt atttgccgtt acaccccgga
61 ctttctgctg acccacaccg atggcaccca gaagttcatc gaggttaagc cgcaaagcaa
121 aattgcggac gaggattttc gtgcgcgttt catcgaaaag caggcgattg cgaaacaaga
181 cggtcgtgat ctgatcctgg tgaccgacaa gcagattcgt gtttacccga ccctgaacaa
241 cctgaaactg ctgcaccgtt atagcggctt tcagagcctg accgagctgc aagcgagcgt
301 gctggaactg gttaagcagt acggtagcat caaagtgggc caactgattc gttatctgaa
361 agttaccgcg ggtgaactgc tggcgaccgt gctgcgtctg ctgagcctgg gccaactgtt
421 cgcggatctg accaccaacg agatcagcat tgaaaccgcg atctggagca acaatgttta
481 ataacgacct gttcgacgat gagtttaacc agccgctgcc gaaggcggaa accaaactgc
541 cgcagaacta taccaaggat ctgcaagcgc tgccggagaa gatcaaaacc accaccttcg
601 cgaagctgaa atacattcaa tggctggagg cgaacatcca gggtggctgg acccaaaaga
661 acctggaacc gctgctgaaa ctgatgccgg acgttgaggg tgaaaagaaa ccgagctggc
721 gtaccgcggc gcgttggtat agcgcgtaca ccaacgcgga taagaacatt atggcgctga
781 tcccgagcca ccagaagaaa ggcaaccgtg aacgtgacac caccaccgat aagttctttg
841 agaaagcgct ggaacgttac ctggtgaagg agaaaccgag cgttgcgagc gcgtataagt
901 tctacaaaga cctggtgatc attgaaaacg acagcgtggt tgatagcgtt ctgaaaccgc
961 tgacctataa ggcgtttaaa aaccgtattg acaacctgcc gcagtatgag gttatgatcg
1021 cgcgttacgg caagcgtctg gcggatattg cgtacaacaa ggtggaaggc cacaaacgtc
1081 cgattcgtgt gctggagaaa gttgaaatcg accacacccc gctggatctg attctgctgg
1141 acgatgagct gcacatcccg ctgggtcgtc cgaccctgac catgctggtt gacgtttata
1201 gccactgcat cgtgggctac tatttcagct ttagcgagcc gagctacgat gcggttcgtc
1261 gtgcgatgct gaacgcgatg aagccgaaaa gcgaagtggc gaaactgtac ccggacacca
1321 ttaacgagtg gaagtgcgcg ggtaaaatcg aaaccctggt ggttgataac ggcgcggagt
1381 tctggagcaa cagcctggaa ctggcgtgcg aggaaatcgg tattaacacc cagtataacc
1441 cggtggcgaa gccgtggctg aaaccgttcg ttgagcgtat gtttggcacc atcaacaccg
1501 aactgctgga cccggttccg ggcaagacct tcagcaacat cctgcaaaaa cacgaataca
1561 acccgaagaa agacgcgatt atgcgtttca ccacctttat gcagctgttt cacaagtggg
1621 tggttgatgt gtatcaccaa gacgcggata gccgtttcaa atacattccg agccagctgt
1681 gggaccaagg ctttaacacc ctgccgccga ccatgctgag cgatgcggat ctgcagcaac
1741 tggatgtggt tctgagcatc agcaaccacc gtgtgctgcg taagggtggc attcgtctgg
1801 agaacctgag ctatgacagc accgaactgg cgaactaccg taagcagttc agccacaaag
1861 tgagccaaga ggttctgatc aaactgaacc cggacgatat tagctacatc tatgtgtacc
1921 tggacaagct ggaacactat attaaagttc cgtgcatcga tccgaacggt tacacccaga
1981 acctgagcct gaaccaacac aagatcaaca ttcgtatcca ccgtgacttt attagcggta
2041 gcatcgataa cgttggcctg gcgaaggcgc gtatgttcat tcacaacaaa atccagaacg
2101 agtttgagga actgaagaac gcgccgaaac acagcaaggt gaaaggtggc aaggcgctgg
2161 cgaaacacca gaacattagc agcgacagcc aaaagagcat cacccacagc aaaccggtgg
2221 aggcgaagaa agttaccccg aaagaacaac cgaccgatag ctgggacgat ttcatcagcg
2281 acctggatgg tttttaatta tgctgaccga caagcagaaa gaaaagctga acgagttccg
2341 tgatgttttt attgaatacc cgatcattac caccatcttc aacgactttg atcgtctgcg
2401 tctgggtaaa ggcctgaccg gcgagaagcc gtgcatgctg ctgaacggtg acaccggcac
2461 cggtaaaacc gcgctgatta aacagtataa ggaacgtcac ctgccgcaat tcatcaacgg
2521 tgttatgaac cacccggtgc tggttagccg tattccgagc aacccgaccc tggaaagcac
2581 cctggcggag ctgctgaaag acctgggtca agtgggcagc accgagcgta agctgcgtat
2641 taacggcacc cgtctgacca ccagcctgat caaatgcctg aagacctgcg gcaccgaact
2701 gatcattatc gatgagtttc aggaactgat tgagcacaac caaggcaaga aacgtcgtga
2761 aattgcgaac cgtctgaaat acatcaacga cgaggcgggt gttagcattg tgctggttgg
2821 catgccgtgg gcggaaaaga tcgcggatga gccgcagtgg agcagccgtc tgctgatccg
2881 tcgtcaactg ccgtatttca aactgagcga gaacccgaag cactttgtgc agctgattat
2941 cggtctggcg aaccgtatgc cgttcgcgga aaaaccgaac ctgagcgagc aagcgaccgt
3001 tttcaccctg tttagcctga gcaaaggctg cttccgtacc ctgaagtact ttctggacga
3061 tgcggtgctg tatgcgctga tggacaacgc gaagaccctg accaccaaac acctggtgaa
3121 ggcgttcgaa gttctgtttc cggatgtgcc gaacctgttt accctgccgg ttgcggagat
3181 caccgcgagc gaggtggaac gttacagcct gtataagccg gaaagcagcc aggacgagga
3241 cccgttcatt gcgaccaaat ttaccgatcg tatgccgatc agccaactgc tgcgtaagta
3301 actcgagccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg
3361 ggttttttgc tgaaacctca ggcatttgag aagcacacgg tcacactgct tccggtagtc
3421 aataaaccgg taaaccagca atagacataa gcggctattt aacgaccctg ccctgaaccg
3481 acgacaagct gacgaccggg tctccgcaag tggcactttt cggggaaatg tgcgcggaac
3541 ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga attaattctt
3601 agaaaaactc atcgagcatc aaatgaaact gcaatttatt catatcagga ttatcaatac
3661 catatttttg aaaaagccgt ttctgtaatg aaggagaaaa ctcaccgagg cagttccata
3721 ggatggcaag atcctggtat cggtctgcga ttccgactcg tccaacatca atacaaccta
3781 ttaatttccc ctcgtcaaaa ataaggttat caagtgagaa atcaccatga gtgacgactg
3841 aatccggtga gaatggcaaa agtttatgca tttctttcca gacttgttca acaggccagc
3901 cattacgctc gtcatcaaaa tcactcgcat caaccaaacc gttattcatt cgtgattgcg
3961 cctgagcgag acgaaatacg cggtcgctgt taaaaggaca attacaaaca ggaatcgaat
4021 gcaaccggcg caggaacact gccagcgcat caacaatatt ttcacctgaa tcaggatatt
4081 cttctaatac ctggaatgct gttttcccgg ggatcgcagt ggtgagtaac catgcatcat
4141 caggagtacg gataaaatgc ttgatggtcg gaagaggcat aaattccgtc agccagttta
4201 gtctgaccat ctcatctgta acatcattgg caacgctacc tttgccatgt ttcagaaaca
4261 actctggcgc atcgggcttc ccatacaatc gatagattgt cgcacctgat tgcccgacat
4321 tatcgcgagc ccatttatac ccatataaat cagcatccat gttggaattt aatcgcggcc
4381 tagagcaaga cgtttcccgt tgaatatggc tcatactctt cctttttcaa tattattgaa
4441 gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata
4501 aacaaatagg catgctagcg cagaaacgtc ctagaagatg ccaggaggat acttagcaga
4561 gagacaataa ggccggagcg aagccgtttt tccataggct ccgcccccct gacgaacatc
4621 acgaaatctg acgctcaaat cagtggtggc gaaacccgac aggactataa agataccagg
4681 cgtttccccc tgatggctcc ctcttgcgct ctcctgttcc cgtcctgcgg cgtccgtgtt
4741 gtggtggagg ctttacccaa atcaccacgt cccgttccgt gtagacagtt cgctccaagc
4801 tgggctgtgt gcaagaaccc cccgttcagc ccgactgctg cgccttatcc ggtaactatc
4861 atcttgagtc caacccggaa agacacgaca aaacgccact ggcagcagcc attggtaact
4921 gagaattagt ggatttagat atcgagagtc ttgaagtggt ggcctaacag aggctacact
4981 gaaaggacag tatttggtat ctgcgctcca ctaaagccag ttaccaggtt aagcagttcc
5041 ccaactgact taaccttcga tcaaaccgcc tccccaggcg gttttttcgt ttacagagca
5101 ggagattacg acgatcgtaa aaggatctca agaagatcct ttacggattc ccgacaccat
5161 cactctagat ttcagtgcaa tttatctctt caaatgtagc acctgaagtc agccccatac
5221 gatataagtt gtaattctca tgttagtcat gccccgcgcc caccggaagg agctgactgg
5281 gttgaaggct ctcaagggca tcggtcgaga tcccggtgcc taatgagtga gctaacttac
5341 attaattgcg ttgcgcctgc ctaatgattt ttatcaaaat cattaaggtt accatcacgg
5401 aaaaaggtta tgctgctttt aagacccact ttcacattta agttgttttt ctaatccgca
5461 tatgatcaat tcaaggccga ataagaaggc tggctctgca ccttggtgat caaataattc
5521 gatagcttgt cgtaataatg gcggcatact atcagtagta ggtgtttccc tttcttcttt
5581 agcgacttga tgctcttgat cttccaatac gcaacctaaa gtaaaatgcc ccacagcgct
5641 gagtgcatat aatgcattct ctagtgaaaa accttgttgg cataaaaagg ctaattgatt
5701 ttcgagagtt tcatactgtt tttctgtagg ccgtgtacct aaatgtactt ttgctccatc
5761 gcgatgactt agtaaagcac atctaaaact tttagcgtta ttacgtaaaa aatcttgcca
5821 gctttcccct tctaaagggc aaaagtgagt atggtgccta tctaacatct caatggctaa
5881 ggcgtcgagc aaagcccgct tattttttac atgccaatac aatgtaggct gctctacacc
5941 tagcttctgg gcgagtttac gggttgttaa accttcgatt ccgacctcat taagcagctc
6001 taatgcgctg ttaatcactt tacttttatc taatctagac atcattaatt cctaattttt
6061 gttgacactc tatcattgat agagttattt taccactccc tatcagtgat agagaaaagt
6121 gaactctaga aataattttg tttaacttta acctgtagaa ataattttgt ttaactttaa
6181 aaggagatat accatgtacc gtcgtaagct gaaatatagc cgtgttaaga acctgcacaa
6241 atttgcgagc cagaagaaca aaagcacctg cctggtggag agcagcctgg aattcgacgc
6301 gtgcttccac tttgagttca gcccgccgat cgcggcgttt ga
//
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