| 质粒名称: | pLPT234 |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 127855 |
| 存储实验室: | Johan Paulsson |
|
载体骨架基本信息 |
|
| 载体名称: | pSC101 |
| 载体出品公司: | -- |
| 空载体大小: | 3000 bp |
| 完整质粒大小: | 9745 bp |
| 载体修饰: | -- |
| 载体类型: | 细菌表达，Synthetic Biology |
| 筛选标记: | -- |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5a |
| 拷贝数: | 低拷贝 |
|
插入基因信息 |
|
| 插入基因名称: | bla,AmpR |
| 物种来源: | E. coli |
| 插入大小: | 861 bp |
| 基因突变: | -- |
| GenBank编号 | -- |
| 启动子: | pAmpR |
| 标签/融合蛋白: | -- |
| 克隆方法: | -- |
| 5’克隆位点: | -- |
| 3’克隆位点: | -- |
| 5’测序引物 | -- |
| 3’测序引物 | -- |
|
文献引用方法 |
|
| 材料和方法部分: | pLPT234 was a gift from Johan Paulsson (Addgene plasmid # 127855 ; http://n2t.net/addgene:127855 ; RRID:Addgene_127855) |
| 参考文献部分: | Bacterial variability in the mammalian gut captured by a single-cell synthetic oscillator. Riglar DT, Richmond DL, Potvin-Trottier L, Verdegaal AA, Naydich AD, Bakshi S, Leoncini E, Lyon LG, Paulsson J, Silver PA. Nat Commun. 2019 Oct 11;10(1):4665. doi: 10.1038/s41467-019-12638-z. 10.1038/s41467-019-12638-z PubMed 31604953 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pLPT234 9745 bp ds-DNA circular SYN 12-MAY-2021
DEFINITION Repressilator 2.0, without degradation tags, integrated triple
reporter.
ACCESSION .
VERSION .
KEYWORDS pLPT234
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 9745)
AUTHORS Riglar DT, Richmond DL, Potvin-Trottier L, Verdegaal AA, Naydich AD,
Bakshi S, Leoncini E, Lyon LG, Paulsson J, Silver PA
TITLE Bacterial variability in the mammalian gut captured by a single-cell
synthetic oscillator.
JOURNAL Nat Commun. 2019 Oct 11;10(1):4665. doi: 10.1038/s41467-019-12638-z.
PUBMED 31604953
REFERENCE 2 (bases 1 to 9745)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..9745
/organism="synthetic DNA construct"
/mol_type="other DNA"
RBS 22..33
/note="strong bacterial ribosome binding site (Elowitz and
Leibler, 2000)"
CDS 40..663
/codon_start=1
/gene="tetR from transposon Tn10"
/product="tetracycline repressor TetR"
/label=TetR
/note="TetR binds to the tetracycline operator tetO to
inhibit transcription. This inhibition can be relieved by
adding tetracycline or doxycycline."
/translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHV
KNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTT
DSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
primer_bind complement(113..132)
/label=Tet-R
/note="Tetracycline resistance gene, reverse primer"
terminator 690..776
/gene="Escherichia coli rrnB"
/label=rrnB T1 terminator
/note="transcription terminator T1 from the E. coli rrnB
gene"
RBS 883..894
/note="strong bacterial ribosome binding site (Elowitz and
Leibler, 2000)"
CDS 912..1994
/codon_start=1
/gene="lacI"
/product="lac repressor"
/label=lacI
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
/translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
ALADSLMQLARQVSRLESGQ"
primer_bind complement(931..950)
/label=LacI-R
/note="LacI, reverse primer"
terminator 2021..2107
/gene="Escherichia coli rrnB"
/label=rrnB T1 terminator
/note="transcription terminator T1 from the E. coli rrnB
gene"
promoter 2138..2211
/label=PLtetO-1 promoter
/note="modified phage lambda PL promoter with tet operator
sites (Lutz and Bujard, 1997)"
protein_bind 2138..2156
/gene="tetO"
/label=tet operator
/bound_moiety="tetracycline repressor TetR"
/note="bacterial operator O2 for the tetR and tetA genes"
protein_bind 2163..2181
/gene="tetO"
/label=tet operator
/bound_moiety="tetracycline repressor TetR"
/note="bacterial operator O2 for the tetR and tetA genes"
RBS 2221..2232
/note="strong bacterial ribosome binding site (Elowitz and
Leibler, 2000)"
CDS 2239..2952
/codon_start=1
/gene="cI"
/product="phage lambda repressor"
/label=-lambda repressor
/translation="MSTKKKPLTQEQLEDARRLKAIYEKKKNELGLSQESVADKMGMGQ
SGVGALFNGINALNAYNAALLAKILKVSVEEFSPSIAREIYEMYEAVSMQPSLRSEYEY
PVFSHVQAGMFSPELRTFTKGDAERWVSTTKKASDSAFWLEVEGNSMTAPTGSKPSFPD
GMLILVDPEQAVEPGDFCIARLGGDEFTFKKLIRDSGQVFLQPLNPQYPMIPCNESCSV
VGKVIASQWPEETFG"
terminator 3010..3096
/gene="Escherichia coli rrnB"
/label=rrnB T1 terminator
/note="transcription terminator T1 from the E. coli rrnB
gene"
CDS complement(3634..4584)
/codon_start=1
/gene="rep101"
/product="RepA protein needed for replication with the
pSC101 origin"
/label=Rep101
/translation="MSELVVFKANELAISRYDLTEHETKLILCCVALLNPTIENPTRKE
RTVSFTYNQYAQMMNISRENAYGVLAKATRELMTRTVEIRNPLVKGFEIFQWTNYAKFS
SEKLELVFSEEILPYLFQLKKFIKYNLEHVKSFENKYSMRIYEWLLKELTQKKTHKANI
EISLDEFKFMLMLENNYHEFKRLNQWVLKPISKDLNTYSNMKLVVDKRGRPTDTLIFQV
ELDRQMDLVTELENNQIKMNGDKIPTTITSDSYLHNGLRKTLHDALTAKIQLTSFEAKF
LSDMQSKYDLNGSFSWLTQKQRTTLENILAKYGRI"
rep_origin 4632..4854
/label=pSC101 ori
/note="low-copy replication origin that requires the Rep101
protein"
terminator 5346..5440
/label=lambda t0 terminator
/note="transcription terminator from phage lambda"
CDS complement(5470..6330)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 6093..6112
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(6331..6435)
/gene="bla"
/label=AmpR promoter
terminator 6574..6660
/gene="Escherichia coli rrnB"
/label=rrnB T1 terminator
/note="transcription terminator T1 from the E. coli rrnB
gene"
RBS 7410..7421
/note="strong bacterial ribosome binding site (Elowitz and
Leibler, 2000)"
protein_bind 7463..7479
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
protein_bind 7486..7502
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
RBS 7749..7760
/note="strong bacterial ribosome binding site (Elowitz and
Leibler, 2000)"
primer_bind complement(8570..8587)
/label=M13 Forward
/note="In lacZ gene. Also called M13-F20 or M13 (-21)
Forward"
primer_bind complement(8570..8586)
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
terminator 8605..8691
/gene="Escherichia coli rrnB"
/label=rrnB T1 terminator
/note="transcription terminator T1 from the E. coli rrnB
gene"
RBS 9474..9485
/note="strong bacterial ribosome binding site (Elowitz and
Leibler, 2000)"
promoter complement(9495..9568)
/label=PLtetO-1 promoter
/note="modified phage lambda PL promoter with tet operator
sites (Lutz and Bujard, 1997)"
protein_bind 9525..9543
/gene="tetO"
/label=tet operator
/bound_moiety="tetracycline repressor TetR"
/note="bacterial operator O2 for the tetR and tetA genes"
protein_bind 9550..9568
/gene="tetO"
/label=tet operator
/bound_moiety="tetracycline repressor TetR"
/note="bacterial operator O2 for the tetR and tetA genes"
protein_bind 9686..9702
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
protein_bind 9709..9725
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
ORIGIN
1 gacgcactga ccgaattcat taaagaggag aaaggtacca tgtccagatt agataaaagt
61 aaagtgatta acagcgcatt agagctgctt aatgaggtcg gaatcgaagg tttaacaacc
121 cgtaaactcg cccagaagct aggtgtagag cagcctacat tgtattggca tgtaaaaaat
181 aagcgggctt tgctcgacgc cttagccatt gagatgttag ataggcacca tactcacttt
241 tgccctttag aaggggaaag ctggcaagat tttttacgta ataacgctaa aagttttaga
301 tgtgctttac taagtcatcg cgatggagca aaagtacatt taggtacacg gcctacagaa
361 aaacagtatg aaactctcga aaatcaatta gcctttttat gccaacaagg tttttcacta
421 gagaatgcat tatatgcact cagcgctgtg gggcatttta ctttaggttg cgtattggaa
481 gatcaagagc atcaagtcgc taaagaagaa agggaaacac ctactactga tagtatgccg
541 ccattattac gacaagctat cgaattattt gatcaccaag gtgcagagcc agccttctta
601 ttcggccttg aattgatcat atgcggatta gaaaaacaac ttaaatgtga aagtgggtct
661 taagctttag cagcttaatc tagaggcatc aaataaaact aaaggctcag tcgaaagact
721 gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct cctgagtagg acaaatccgc
781 cgccctagac ctagctgcag gtcgaggata aatatctaac accgtgcgtg ttgactattt
841 tacctctggc ggtgataatg gttgcatgta ctagaattca ttaaagagga gaaaggtacc
901 atatggtgaa tgtgaaacca gtaacgttat acgatgtcgc agagtatgcc ggtgtctctt
961 atcagaccgt ttcccgcgtg gtgaaccagg ccagccacgt ttctgcgaaa acgcgggaaa
1021 aagtggaagc ggcgatggcg gagctgaatt acattcccaa ccgcgtggca caacaactgg
1081 cgggcaaaca gtcgttgctg attggcgttg ccacctccag tctggccctg cacgcgccgt
1141 cgcaaattgt cgcggcgatt aaatctcgcg ccgatcaact gggtgccagc gtggtggtgt
1201 cgatggtaga acgaagcggc gtcgaagcct gtaaagcggc ggtgcacaat cttctcgcgc
1261 aacgcgtcag tgggctgatc attaactatc cgctggatga ccaggatgcc attgctgtgg
1321 aagctgcctg cactaatgtt ccggcgttat ttcttgatgt ctctgaccag acacccataa
1381 acagtattat tttctcccat gaagacggta cgcgactggg cgtggagcat ctggtcgcat
1441 tgggtcacca gcaaatcgcg ctgttagcgg gcccattaag ttctgtctcg gcgcgtctgc
1501 gtctggctgg ctggcataaa tatctcactc gcaatcaaat tcagccgata gcggaacggg
1561 aaggcgactg gagtgccatg tccggttttc aacaaaccat gcaaatgctg aatgagggca
1621 tcgttccaac tgcgatgctg gttgccaacg atcagatggc gctgggcgca atgcgcgcca
1681 ttaccgagtc cgggctgcgc gttggtgcgg atatctcggt agtgggatac gacgataccg
1741 aagacagctc atgttatatc ccgccgttaa ccaccatcaa acaggatttt cgcctgctgg
1801 ggcaaaccag cgtggaccgc ttgctgcaac tctctcaggg ccaggcggtg aagggcaatc
1861 agctgttgcc cgtctcactg gtgaaaagaa aaaccaccct ggcgcccaat acgcaaaccg
1921 cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt tcccgactgg
1981 aaagcgggca gtaagcttaa ttagctgagt ctagaggcat caaataaaac gaaaggctca
2041 gtcgaaagac tgggcctttc gttttatctg ttgtttgtcg gtgaacgctc tcctgagtag
2101 gacaaatccg ccgccctaga cctagctgca ggtcgagtcc ctatcagtga tagagattga
2161 catccctatc agtgatagag atactgagca catcagcagg acgcactgac cgaattcatt
2221 aaagaggaga aaggtaccat gagcacaaaa aagaaaccat taacacaaga gcagcttgag
2281 gacgcacgtc gccttaaagc aatttatgaa aaaaagaaaa atgaacttgg cttatcccag
2341 gaatctgtcg cagacaagat ggggatgggg cagtcaggcg ttggtgcttt atttaatggc
2401 atcaatgcat taaatgctta taacgccgca ttgcttgcaa aaattctcaa agttagcgtt
2461 gaagaattta gcccttcaat cgccagagaa atctacgaga tgtatgaagc ggttagtatg
2521 cagccgtcac ttagaagtga gtatgagtac cctgtttttt ctcatgttca ggcagggatg
2581 ttctcacctg agcttagaac ctttaccaaa ggtgatgcgg agagatgggt aagcacaacc
2641 aaaaaagcca gtgattctgc attctggctt gaggttgaag gtaattccat gaccgcacca
2701 acaggctcca agccaagctt tcctgacgga atgttaattc tcgttgaccc tgagcaggct
2761 gttgagccag gtgatttctg catagccaga cttgggggtg atgagtttac cttcaagaaa
2821 ctgatcaggg atagcggtca ggtgttttta caaccactaa acccacagta cccaatgatc
2881 ccatgcaatg agagttgttc cgttgtgggg aaagttatcg ctagtcagtg gcctgaagag
2941 acgtttggct gaaaactacg ctttagcagc ttgatctaga ggatcccatg gtacgcgtgc
3001 tagaggcatc aaataaaacg aaaggctcag tcgaaagact gggcctttcg ttttatctgt
3061 tgtttgtcgg tgaacgctct cctgagtagg acaaatccgc cgccctagac ctagggtacg
3121 ggttttgctg cccgcaaacg ggctgttctg gtgttgctag tttgttatca gaatcgcaga
3181 tccggcttca gccggtttgc cggctgaaag cgctatttct tccagaattg ccatgatttt
3241 ttccccacgg gaggcgtcac tggctcccgt gttgtcggca gctttgattc gataagcagc
3301 atcgcctgtt tcaggctgtc tatgtgtgac tgttgagctg taacaagttg tctcaggtgt
3361 tcaatttcat gttctagttg ctttgtttta ctggtttcac ctgttctatt aggtgttaca
3421 tgctgttcat ctgttacatt gtcgatctgt tcatggtgaa cagctttgaa tgcaccaaaa
3481 actcgtaaaa gctctgatgt atctatcttt tttacaccgt tttcatctgt gcatatggac
3541 agttttccct ttgatatgta acggtgaaca gttgttctac ttttgtttgt tagtcttgat
3601 gcttcactga tagatacaag agccataaga acctcagatc cttccgtatt tagccagtat
3661 gttctctagt gtggttcgtt gtttttgcgt gagccatgag aacgaaccat tgagatcata
3721 cttactttgc atgtcactca aaaattttgc ctcaaaactg gtgagctgaa tttttgcagt
3781 taaagcatcg tgtagtgttt ttcttagtcc gttatgtagg taggaatctg atgtaatggt
3841 tgttggtatt ttgtcaccat tcatttttat ctggttgttc tcaagttcgg ttacgagatc
3901 catttgtcta tctagttcaa cttggaaaat caacgtatca gtcgggcggc ctcgcttatc
3961 aaccaccaat ttcatattgc tgtaagtgtt taaatcttta cttattggtt tcaaaaccca
4021 ttggttaagc cttttaaact catggtagtt attttcaagc attaacatga acttaaattc
4081 atcaaggcta atctctatat ttgccttgtg agttttcttt tgtgttagtt cttttaataa
4141 ccactcataa atcctcatag agtatttgtt ttcaaaagac ttaacatgtt ccagattata
4201 ttttatgaat ttttttaact ggaaaagata aggcaatatc tcttcactaa aaactaattc
4261 taatttttcg cttgagaact tggcatagtt tgtccactgg aaaatctcaa agcctttaac
4321 caaaggattc ctgatttcca cagttctcgt catcagctct ctggttgctt tagctaatac
4381 accataagca ttttccctac tgatgttcat catctgagcg tattggttat aagtgaacga
4441 taccgtccgt tctttccttg tagggttttc aatcgtgggg ttgagtagtg ccacacagca
4501 taaaattagc ttggtttcat gctccgttaa gtcatagcga ctaatcgcta gttcatttgc
4561 tttgaaaaca actaattcag acatacatct caattggtct aggtgatttt aatcactata
4621 ccaattgaga tgggctagtc aatgataatt actagtcctt ttcctttgag ttgtgggtat
4681 ctgtaaattc tgctagacct ttgctggaaa acttgtaaat tctgctagac cctctgtaaa
4741 ttccgctaga cctttgtgtg ttttttttgt ttatattcaa gtggttataa tttatagaat
4801 aaagaaagaa taaaaaaaga taaaaagaat agatcccagc cctgtgtata actcactact
4861 ttagtcagtt ccgcagtatt acaaaaggat gtcgcaaacg ctgtttgctc ctctacaaaa
4921 cagaccttaa aaccctaaag gcttaagtag caccctcgca agctcgggca aatcgctgaa
4981 tattcctttt gtctccgacc atcaggcacc tgagtcgctg tctttttcgt gacattcagt
5041 tcgctgcgct cacggctctg gcagtgaatg ggggtaaatg gcactacagg cgccttttat
5101 ggattcatgc aaggaaacta cccataatac aagaaaagcc cgtcacgggc ttctcagggc
5161 gttttatggc gggtctgcta tgtggtgcta tctgactttt tgctgttcag cagttcctgc
5221 cctctgattt tccagtctga ccacttcgga ttatcccgtg acaggtcatt cagactggct
5281 aatgcaccca gtaaggcagc ggtatcatca acaggcttac ccgtcttact gtccctagtg
5341 cttggattct caccaataaa aaacgcccgg cggcaaccga gcgttctgaa caaatccaga
5401 tggagttctg aggtcattac tggatctatc aacaggagtc caagcgagct cgtaaacttg
5461 gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg
5521 ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc
5581 atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc
5641 agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc
5701 ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag
5761 tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat
5821 ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg
5881 caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt
5941 gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag
6001 atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg
6061 accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt
6121 aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct
6181 gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac
6241 tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat
6301 aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat
6361 ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca
6421 aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtctctt tcctgcgtta
6481 tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc
6541 agcccagcct acaccccggg tctagggcgg cggatttgtc ctactcagga gagcgttcac
6601 cgacaaacaa cagataaaac gaaaggccca gtctttcgac tgagcctttc gttttatttg
6661 atgcctctag actcagctaa ttaagcttat ttgtatagtt catccatgcc atgtgtaatc
6721 ccagcagctg ttacaaactc aagaaggacc atgtggtctc tcttttcgtt gggatctttc
6781 gaaagcttag attgtgtgga caggtaatgg ttgtctggta aaaggacagg gccatcgcca
6841 attggagtat tttgttgata atggtctgct agttgaacgc ctccatcttc aatgttgtgt
6901 ctaattttga agttagcttt gattccattc ttttgtttgt ctgccgtgat gtatacattg
6961 tctgagatgt agttgtattc caatttgtgt ccaagaatgt ttccatcttc tttaaaatca
7021 atacctttta actcgattct attaacaagg gtatcacctt caaacttgac ttcagcacgt
7081 gtcttgtagt tcccgtcatc tttgaaaaat atagttcttt cctgtacata accttcgggc
7141 atggcactct tgaaaaagtc atgctgtttc atatgatctg ggtatcttgc aaagcattga
7201 acaccccaag tgagagtagt gacaagtgtt ggccatggaa caggtagttt tccagtagtg
7261 caaataaatt taagggtaag ttttccgtat gttgcatcac cttcaccctc tccactgaca
7321 gaaaatttgt gcccattaac atcaccatct aattcaacaa gaattgggac aactccagtg
7381 aaaagttctt ctcctttact catggtacct ttctcctctt taatgaattc ggtcagtgcg
7441 tcctgctgat gtgctcagta tcttgttatc cgctcacaat gtcaattgtt atccgctcac
7501 aattctcgag gtgaagacga aagggcctcg tgatacgcct atttttatag gttaatgtca
7561 tgataataat ggtttcttag acgtcaggtg gcacttttcg ggtcacaaaa gacattccac
7621 caaacgtcgt ggcggctggc gttccttgga taaatatcta acaccgtgcg tgttgactat
7681 tttacctctg gcggtgataa tggttgcatg tactagaata tcagcaggac gcactgaccg
7741 aattcattaa agaggagaaa ggtaccgcat ggttagtaaa ggagaagaaa ataacatggc
7801 actgattaag gagaacatgc acatgaagct gtacatggag ggcaccgtga acaaccacca
7861 cttcaagtgc acatccgagg gcgaaggcaa gccctacgag ggcacccaga ccatgagaat
7921 caaggccgtc gagggcggcc ctctcccctt cgccttcgac atcctggcta ccagcttcat
7981 gtacggcagc aaaaccttca tcaaccacac ccagggcatc cccgacttct ttaagcagtc
8041 cttccctgag ggcttcacat gggagagagt caccacatac gaagacgggg gcgtgctgac
8101 cgctacccag gacaccagcc tccaggacgg ctgcctcatc tacaacgtca agatcagagg
8161 ggtgaacttc ccatccaacg gccctgtgat gcagaagaaa acactcggct gggaggcctc
8221 caccgagacc ctgtaccccg ctgacggcgg ccttgaaggc agagccgaca tggccctgaa
8281 gctcgtgggc gggggccacc tgatctgcaa cttgaagacc acatacagat ccaagaaacc
8341 cgctaagaac ctcaagatgc ccggcgtcta ctatgtggac agaagactgg aaagaatcaa
8401 ggaggccgac aaagagacct acgtcgagca gcacgaggtg gctgtggcca gatactgcga
8461 cctccctagc aaactggggc acagagtcta ggggatcccc cgggctgcag gaattcgata
8521 tcacgcaaaa aaccccgctt cggcggggtt ttttcgcgag ctcgaattca ctggccgtcg
8581 ttttacatag gtctagggcg gcggatttgt cctactcagg agagcgttca ccgacaaaca
8641 acagataaaa cgaaaggccc agtctttcga ctgagccttt cgttttattt gatgcctcta
8701 gcacgcgtac aatgggatcc cccgggctgc aggaattcga tatcaagctt atttgtatag
8761 ttcatccatg ccatgtgtaa tcccagcagc tgttacaaac tcaagaagga ccatgtggtc
8821 tctcttttcg ttgggatctt tcgaaagctt agattggtag gacaggtaat ggttgtctgg
8881 taaaaggaca gggccatcgc caattggagt attttgttga taatggtctg ctagttgaac
8941 acctccatct tcaatgttgt gtctaatttt gaagttcgct ttgattccat tcttttgttt
9001 gtctgccgtg atgtatacat tgtgtgagtt atagttgtat tccaatttgt gtccaagaat
9061 gtttccatct tctttaaaat caataccttt taactcgatt ctattaacaa gggtatcacc
9121 ttcaaacttg acttcagcac gtgtcttgta gttcccgtca tctttgaaaa atatagttct
9181 ttcctgtaca taaccttcgg gcatggcact cttgaaaaag tcatgctgtt tcatatgatc
9241 tgggtatctc gcaaagcatt gaacaccata acccaaagta gtgacaagtg ttggccatgg
9301 aacaggtagt tttccagtag tgcaaatcaa tttaagggta agttttccgt atgttgcatc
9361 accttcaccc tctccactga cagaaaattt gtgcccatta acatcaccat ctaattcaac
9421 aagaattggg acaactccag tgaaaagttc ttctccttta ctcatgcggt acctttctcc
9481 tctttaatga attcggtcag tgcgtcctgc tgatgtgctc agtatctcta tcactgatag
9541 ggatgtcaat ctctatcact gatagggact cgaggtgaag acgaaagggc ctcgtgctcg
9601 agtacgtcta agaaaccatt attatcatga cattaaccta taaaaatagg cgtatcacga
9661 ggccctttcg tcttcacctc gagaattgtg agcggataac aattgacatt gtgagcggat
9721 aacaagatac tgagcacatc agcag
//
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