pProEX HTb

价格:900元

联系方式:I47-825O-882O

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pProEXHTb载体基本信息

出品公司: EMD Biosciences(Novagen)
别名: pProEX HTb, pProEX HTb
质粒类型: 大肠杆菌蛋白表达
表达水平:
克隆方法: 多克隆位点,限制性内切酶
载体大小: 4779bp
5' 测序引物序列: M13/pUC Reverse: AGCGGATAACAATTTCACACAGG (Invitrogen)
3' 测序引物序列:   pTrcHis Reverse: GATTTAATCTGTATCAGG (Invitrogen)
载体标签: N-His, N-TEV 蛋白酶切位点
载体抗性: 氨苄
备注:
Hosts: E.coli. Related vectors: pBR322.
产品目录号: 50610
稳定性: 瞬时表达 Transient
组成型/诱导型: 诱导型
病毒/非病毒: 非病毒

pProEXHTb载体质粒图谱和多克隆位点信息

pProEX HTb载体图谱
pProEX HTb多克隆位点

pProEXHTb载体简介

pProEX HT系列原核表达系统是用来在大肠杆菌中表达外源蛋白的载体。使用该载体表达的目的蛋白含有6个His纯化标签。目的基因可以克隆到载体的多克隆位点处,可以选择的载体有pProEX HTa, b, c.蛋白表达后,His纯化标签就位于目的蛋白的N端,同时His纯化标签的使用可以用于蛋白纯化。载体上同时含有rTEV蛋白酶识别位点,可以使用rTEV蛋白酶进行酶切,从而去除融合的His标签。

pProEXHTb载体序列

LOCUS       pPROEX HTb	4779 bp 	DNA    circular 	SYN
DEFINITION  pPROEX HTb
ACCESSION   
KEYWORDS    
SOURCE      
  ORGANISM  other sequences; artificial sequences; vectors.
FEATURES             Location/Qualifiers
     source          1..4779
                     /organism="pPROEX HTb"
                     /mol_type="other DNA"
     promoter        193..266
                     /label="trc_promoter"
     misc_feature    235..257
                     /label="M13_pUC_rev_primer"
     misc_feature    complement(473..490)
                     /label="pTrcHis_rev_primer"
     misc_feature    complement(473..490)
                     /label="pBAD_rev_primer"
     terminator      523..680
                     /label="rrnB_terminator"
     terminator      646..689
                     /label="rrnB_T1_terminator"
     terminator      821..848
                     /label="rrnB_T2_terminator"
     promoter        890..918
                     /label="AmpR_promoter"
     gene            960..1820
                     /label="Ampicillin"
                     /gene="Ampicillin"
     CDS             960..1820
                     /label="ORF frame 3"
     rep_origin      1975..2594
                     /label="pBR322_origin"
     rep_origin      complement(2932..3238)
                     /label="f1_origin"
     misc_feature    3480..3502
                     /label="pGEX_3_primer"
     misc_feature    3649..4740
                     /label="lacI"
     CDS             3781..4740
                     /label="ORF frame 1"
ORIGIN
    1 GTTTGACAGC TTATCATCGA CTGCACGGTG CACCAATGCT TCTGGCGTCA GGCAGCCATC
   61 GGAAGCTGTG GTATGGCTGT GCAGGTCGTA AATCACTGCA TAATTCGTGT CGCTCAAGGC
  121 GCACTCCCGT TCTGGATAAT GTTTTTTGCG CCGACATCAT AACGGTTCTG GCAAATATTC
  181 TGAAATGAGC TGTTGACAAT TAATCATCCG GTCCGTATAA TCTGTGGAAT TGTGAGCGGA
  241 TAACAATTTC ACACAGGAAA CAGACCATGT CGTACTACCA TCACCATCAC CATCACGATT
  301 ACGATATCCC AACGACCGAA AACCTGTATT TTCAGGGCGC CATGGGATCC GGAATTCAAA
  361 GGCCTACGTC GACGAGCTCA CTAGTCGCGG CCGCTTTCGA ATCTAGAGCC TGCAGTCTCG
  421 AGGCATGCGG TACCAAGCTT GGCTGTTTTG GCGGATGAGA GAAGATTTTC AGCCTGATAC
  481 AGATTAAATC AGAACGCAGA AGCGGTCTGA TAAAACAGAA TTTGCCTGGC GGCAGTAGCG
  541 CGGTGGTCCC ACCTGACCCC ATGCCGAACT CAGAAGTGAA ACGCCGTAGC GCCGATGGTA
  601 GTGTGGGGTC TCCCCATGCG AGAGTAGGGA ACTGCCAGGC ATCAAATAAA ACGAAAGGCT
  661 CAGTCGAAAG ACTGGGCCTT TCGTTTTATC TGTTGTTTGT CGGTGAACGC TCTCCTGAGT
  721 AGGACAAATC CGCCGGGAGC GGATTTGAAC GTTGCGAAGC AACGGCCCGG AGGGTGGCGG
  781 GCAGGACGCC CGCCATAAAC TGCCAGGCAT CAAATTAAGC AGAAGGCCAT CCTGACGGAT
  841 GGCCTTTTTG CGTTTCTACA AACTCTTTTT GTTTATTTTT CTAAATACAT TCAAATATGT
  901 ATCCGCTCAT GAGACAATAA CCCTGATAAA TGCTTCAATA ATATTGAAAA AGGAAGAGTA
  961 TGAGTATTCA ACATTTCCGT GTCGCCCTTA TTCCCTTTTT TGCGGCATTT TGCCTTCCTG
 1021 TTTTTGCTCA CCCAGAAACG CTGGTGAAAG TAAAAGATGC TGAAGATCAG TTGGGTGCAC
 1081 GAGTGGGTTA CATCGAACTG GATCTCAACA GCGGTAAGAT CCTTGAGAGT TTTCGCCCCG
 1141 AAGAACGTTT TCCAATGATG AGCACTTTTA AAGTTCTGCT ATGTGGCGCG GTATTATCCC
 1201 GTGTTGACGC CGGGCAAGAG CAACTCGGTC GCCGCATACA CTATTCTCAG AATGACTTGG
 1261 TTGAGTACTC ACCAGTCACA GAAAAGCATC TTACGGATGG CATGACAGTA AGAGAATTAT
 1321 GCAGTGCTGC CATAACCATG AGTGATAACA CTGCGGCCAA CTTACTTCTG ACAACGATCG
 1381 GAGGACCGAA GGAGCTAACC GCTTTTTTGC ACAACATGGG GGATCATGTA ACTCGCCTTG
 1441 ATCGTTGGGA ACCGGAGCTG AATGAAGCCA TACCAAACGA CGAGCGTGAC ACCACGATGC
 1501 CTACAGCAAT GGCAACAACG TTGCGCAAAC TATTAACTGG CGAACTACTT ACTCTAGCTT
 1561 CCCGGCAACA ATTAATAGAC TGGATGGAGG CGGATAAAGT TGCAGGACCA CTTCTGCGCT
 1621 CGGCCCTTCC GGCTGGCTGG TTTATTGCTG ATAAATCTGG AGCCGGTGAG CGTGGGTCTC
 1681 GCGGTATCAT TGCAGCACTG GGGCCAGATG GTAAGCCCTC CCGTATCGTA GTTATCTACA
 1741 CGACGGGGAG TCAGGCAACT ATGGATGAAC GAAATAGACA GATCGCTGAG ATAGGTGCCT
 1801 CACTGATTAA GCATTGGTAA CTGTCAGACC AAGTTTACTC ATATATACTT TAGATTGATT
 1861 TAAAACTTCA TTTTTAATTT AAAAGGATCT AGGTGAAGAT CCTTTTTGAT AATCTCATGA
 1921 CCAAAATCCC TTAACGTGAG TTTTCGTTCC ACTGAGCGTC AGACCCCGTA GAAAAGATCA
 1981 AAGGATCTTC TTGAGATCCT TTTTTTCTGC GCGTAATCTG CTGCTTGCAA ACAAAAAAAC
 2041 CACCGCTACC AGCGGTGGTT TGTTTGCCGG ATCAAGAGCT ACCAACTCTT TTTCCGAAGG
 2101 TAACTGGCTT CAGCAGAGCG CAGATACCAA ATACTGTCCT TCTAGTGTAG CCGTAGTTAG
 2161 GCCACCACTT CAAGAACTCT GTAGCACCGC CTACATACCT CGCTCTGCTA ATCCTGTTAC
 2221 CAGTGGCTGC TGCCAGTGGC GATAAGTCGT GTCTTACCGG GTTGGACTCA AGACGATAGT
 2281 TACCGGATAA GGCGCAGCGG TCGGGCTGAA CGGGGGGTTC GTGCACACAG CCCAGCTTGG
 2341 AGCGAACGAC CTACACCGAA CTGAGATACC TACAGCGTGA GCTATGAGAA AGCGCCACGC
 2401 TTCCCGAAGG GAGAAAGGCG GACAGGTATC CGGTAAGCGG CAGGGTCGGA ACAGGAGAGC
 2461 GCACGAGGGA GCTTCCAGGG GGAAACGCCT GGTATCTTTA TAGTCCTGTC GGGTTTCGCC
 2521 ACCTCTGACT TGAGCGTCGA TTTTTGTGAT GCTCGTCAGG GGGGCGGAGC CTATGGAAAA
 2581 ACGCCAGCAA CGCGGCCTTT TTACGGTTCC TGGCCTTTTG CTGGCCTTTT GCTCACATGT
 2641 TCTTTCCTGC GTTATCCCCT GATTCTGTGG ATAACCGTAT TACCGCCTTT GAGTGAGCTG
 2701 ATACCGCTCG CCGCAGCCGA ACGACCGAGC GCAGCGAGTC AGTGAGCGAG GAAGCGGAAG
 2761 AGCGCCTGAT GCGGTATTTT CTCCTTACGC ATCTGTGCGG TATTTCACAC CGCATAATTT
 2821 TGTTAAAATT CGCGTTAAAT TTTTGTTAAA TCAGCTCATT TTTTAACCAA TAGGCCGAAA
 2881 TCGGCAAAAT CCCTTATAAA TCAAAAGAAT AGACCGAGAT AGGGTTGAGT GTTGTTCCAG
 2941 TTTGGAACAA GAGTCCACTA TTAAAGAACG TGGACTCCAA CGTCAAAGGG CGAAAAACCG
 3001 TCTATCAGGG CGATGGCCCA CTACGTGAAC CATCACCCTA ATCAAGTTTT TTGGGGTCGA
 3061 GGTGCCGTAA AGCACTAAAT CGGAACCCTA AAGGGAGCCC CCGATTTAGA GCTTGACGGG
 3121 GAAAGCCGGC GAACGTGGCG AGAAAGGAAG GGAAGAAAGC GAAAGGAGCG GGCGCTAGGG
 3181 CGCTGGCAAG TGTAGCGGTC ACGCTGCGCG TAACCACCAC ACCCGCCGCG CTTAATGCGC
 3241 CGCTACAGGG CGCGTCCCAT TCGCCATTCA GGCTGCTATG GTGCACTCTC AGTACAATCT
 3301 GCTCTGATGC CGCATAGTTA AGCCAGTACC AGTCACGTAG CGATATCGGA GTGTATACAC
 3361 TCCGCTATCG CTACGTGACT GGGTCATGGC TGCGCCCCGA CACCCGCCAA CACCCGCTGA
 3421 CGCGCCCTGA CGGGCTTGTC TGCTCCCGGC ATCCGCTTAC AGACAAGCTG TGACCGTCTC
 3481 CGGGAGCTGC ATGTGTCAGA GGTTTTCACC GTCATCACCG AAACGCGCGA GGCAGCAGAT
 3541 CAATTCGCGC GCGAAGGCGA AGCGGCATGC ATTTACGTTG ACACCATCGA ATGGTGCAAA
 3601 ACCTTTCGCG GTATGGCATG ATAGCGCCCG GAAGAGAGTC AATTCAGGGT GGTGAATGTG
 3661 AAACCAGTAA CGTTATACGA TGTCGCAGAG TATGCCGGTG TCTCTTATCA GACCGTTTCC
 3721 CGCGTGGTGA ACCAGGCCAG CCACGTTTCT GCGAAAACGC GGGAAAAAGT GGAAGCGGCG
 3781 ATGGCGGAGC TGAATTACAT TCCCAACCGC GTGGCACAAC AACTGGCGGG CAAACAGTCG
 3841 TTGCTGATTG GCGTTGCCAC CTCCAGTCTG GCCCTGCACG CGCCGTCGCA AATTGTCGCG
 3901 GCGATTAAAT CTCGCGCCGA TCAACTGGGT GCCAGCGTGG TGGTGTCGAT GGTAGAACGA
 3961 AGCGGCGTCG AAGCCTGTAA AGCGGCGGTG CACAATCTTC TCGCGCAACG CGTCAGTGGG
 4021 CTGATCATTA ACTATCCGCT GGATGACCAG GATGCCATTG CTGTGGAAGC TGCCTGCACT
 4081 AATGTTCCGG CGTTATTTCT TGATGTCTCT GACCAGACAC CCATCAACAG TATTATTTTC
 4141 TCCCATGAAG ACGGTACGCG ACTGGGCGTG GAGCATCTGG TCGCATTGGG TCACCAGCAA
 4201 ATCGCGCTGT TAGCGGGCCC ATTAAGTTCT GTCTCGGCGC GTCTGCGTCT GGCTGGCTGG
 4261 CATAAATATC TCACTCGCAA TCAAATTCAG CCGATAGCGG AACGGGAAGG CGACTGGAGT
 4321 GCCATGTCCG GTTTTCAACA AACCATGCAA ATGCTGAATG AGGGCATCGT TCCCACTGCG
 4381 ATGCTGGTTG CCAACGATCA GATGGCGCTG GGCGCAATGC GCGCCATTAC CGAGTCCGGG
 4441 CTGCGCGTTG GTGCGGATAT CTCGGTAGTG GGATACGACG ATACCGAAGA CAGCTCATGT
 4501 TATATCCCGC CGTTAACCAC CATCAAACAG GATTTTCGCC TGCTGGGGCA AACCAGCGTG
 4561 GACCGCTTGC TGCAACTCTC TCAGGGCCAG GCGGTGAAGG GCAATCAGCT GTTGCCCGTC
 4621 TCACTGGTGA AAAGAAAAAC CACCCTGGCA CCCAATACGC AAACCGCCTC TCCCCGCGCG
 4681 TTGGCCGATT CATTAATGCA GCTGGCACGA CAGGTTTCCC GACTGGAAAG CGGGCAGTGA
 4741 GCGCAACGCA ATTAATGTGA GTTAGCGCGA ATTGATCTG
//

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