pGL4.51[luc2/CMV/Neo]

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pGL4.51载体基本信息

出品公司: Promega
载体名称: pGL4.51 ; pGL4.51[luc2/CMV/Neo]
质粒类型: 信号通路报告载体;哺乳动物载体;荧光素酶报告载体
高拷贝/低拷贝: --
克隆方法: 限制性内切酶,多克隆位点
启动子: CMV
载体大小: 6358 bp
5' 测序引物及序列: CMV-F: TCTAAAAGCTGCGGAATTGT
3' 测序引物及序列: --
载体标签: luc2
载体抗性: 氨苄青霉素
筛选标记: 新霉素(Neomycin)
克隆菌株: TOP10等常规菌株
宿主细胞(系): HEK293等
备注: pGL4.51[luc2/CMV/Neo]载体是信号通路报告载体;含ARE应答元件;含荧光 素酶报告基因luc2。
产品目录号: E1320
稳定性: 瞬表达或稳表达
组成型/诱导型: 组成型
病毒/非病毒: 非病毒

pGL4.51载体质粒图谱和多克隆位点信息

pGL4.51载体图谱

pGL4.51 载体特征

pGL4.51载体简介

pGL4.51载体简介
The pGL4.51[luc2/CMV/Neo] Vector encodes the luciferase reporter gene luc2 (Photinus pyralis), which has been codon optimized for mammalian expression. This vector is also engineered with fewer consensus regulatory sequences for reduced backgrounds and a decreased risk of anomalous transcription. This vector contains the following features: luc2 reporter gene for expression in mammalian cells; CMV promoter for high translational expression; SV40 late poly(A) signal sequence is positioned downstream of luc2 to provide efficient transcription termination and mRNA polyadenylation; Binding region for RVprimer 3 and RVprimer 4; Synthetic poly(A) signal/transcription start site; Synthetic Neomycin-resistance gene for mammalian cell selection of the plasmid; Plasmid replication origin; Ampr gene for bacterial selection for vector amplification.

pGL4.51载体序列

LOCUS       EU921841                6358 bp    DNA     linear   SYN 28-AUG-2008
DEFINITION  Firefly luciferase reporter vector pGL4.51[luc2/CMV/Neo], complete
            sequence.
ACCESSION   EU921841
VERSION     EU921841.1  GI:197215836
KEYWORDS    .
SOURCE      Firefly luciferase reporter vector pGL4.51[luc2/CMV/Neo]
  ORGANISM  Firefly luciferase reporter vector pGL4.51[luc2/CMV/Neo]
            other sequences; artificial sequences; vectors.
REFERENCE   1  (bases 1 to 6358)
  AUTHORS   Brouette,C., Hung,L. and Kopish,K.
  TITLE     pGL4 Luciferase Reporter Vectors Technical Manual, TM259
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6358)
  AUTHORS   Brouette,C., Hung,L. and Kopish,K.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUL-2008) SciComm, Promega, 5500 E Cheryl Parkway,
            Madison, WI 53711, USA
FEATURES             Location/Qualifiers
     source          1..6358
                     /organism="Firefly luciferase reporter vector
                     pGL4.51[luc2/CMV/Neo]"
                     /mol_type="other DNA"
                     /db_xref="taxon:554673"
     regulatory      14..755
                     /regulatory_class="promoter"
                     /note="CMV immediate early enhancer/promoter"
     gene            859..2511
                     /gene="luc2"
     CDS             859..2511
                     /gene="luc2"
                     /note="luminescent reporter protein; derived from Photinus
                     pyralis"
                     /codon_start=1
                     /transl_table=11
                     /product="firefly luciferase"
                     /protein_id="ACH53166.1"
                     /db_xref="GI:197215837"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTD
                     AHIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAV
                     APANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQG
                     FQSMYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVR
                     FSHARDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRS
                     LQDYKIQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLP
                     GIRQGYGLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCV
                     RGPMIMSGYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVA
                     PAELESILLQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTT
                     AKKLRGGVVFVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     misc_feature    2546..2767
                     /note="SV40 late poly(A) region"
     regulatory      2815..3233
                     /regulatory_class="promoter"
                     /note="SV40 early enhancer/promoter"
     gene            3258..4052
                     /gene="NeoR"
     CDS             3258..4052
                     /gene="NeoR"
                     /note="antibiotic resistance protein"
                     /codon_start=1
                     /transl_table=11
                     /product="neomycin phosphotransferase"
                     /protein_id="ACH53167.1"
                     /db_xref="GI:197215838"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGR
                     PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDL
                     LSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                     EHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRY
                     QDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     misc_feature    4077..4125
                     /note="poly(A) region"
     primer_bind     complement(4192..4211)
                     /note="RV primer 4 binding region"
     gene            5240..6100
                     /gene="AmpR"
     CDS             complement(5240..6100)
                     /note="confers resistance to ampicillin"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase"
                     /protein_id="ACH53168.1"
                     /db_xref="GI:197215839"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW"
     misc_feature    6205..6358
                     /note="poly(A) signal/transcription pause region"
     primer_bind     6307..6326
                     /note="RV primer 3 binding region"
ORIGIN      
        1 ggcctaactg gcctcaatat tggccattag ccatattatt cattggttat atagcataaa
       61 tcaatattgg ctattggcca ttgcatacgt tgtatctata tcataatatg tacatttata
      121 ttggctcatg tccaatatga ccgccatgtt ggcattgatt attgactagt tattaatagt
      181 aatcaattac ggggtcatta gttcatagcc catatatgga gttccgcgtt acataactta
      241 cggtaaatgg cccgcctggc tgaccgccca acgacccccg cccattgacg tcaataatga
      301 cgtatgttcc catagtaacg ccaataggga ctttccattg acgtcaatgg gtggagtatt
      361 tacggtaaac tgcccacttg gcagtacatc aagtgtatca tatgccaagt ccgcccccta
      421 ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc ccagtacatg accttacggg
      481 actttcctac ttggcagtac atctacgtat tagtcatcgc tattaccatg gtgatgcggt
      541 tttggcagta caccaatggg cgtggatagc ggtttgactc acggggattt ccaagtctcc
      601 accccattga cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat
      661 gtcgtaataa ccccgccccg ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtct
      721 atataagcag agctcgttta gtgaaccgtc agatcactag aagctttatt gcggtagttt
      781 atcacagtta aattgctaac gcagtcagtg ggcctcggcg gccaagcttg gcaatccggt
      841 actgttggta aagccaccat ggaagatgcc aaaaacatta agaagggccc agcgccattc
      901 tacccactcg aagacgggac cgccggcgag cagctgcaca aagccatgaa gcgctacgcc
      961 ctggtgcccg gcaccatcgc ctttaccgac gcacatatcg aggtggacat tacctacgcc
     1021 gagtacttcg agatgagcgt tcggctggca gaagctatga agcgctatgg gctgaataca
     1081 aaccatcgga tcgtggtgtg cagcgagaat agcttgcagt tcttcatgcc cgtgttgggt
     1141 gccctgttca tcggtgtggc tgtggcccca gctaacgaca tctacaacga gcgcgagctg
     1201 ctgaacagca tgggcatcag ccagcccacc gtcgtattcg tgagcaagaa agggctgcaa
     1261 aagatcctca acgtgcaaaa gaagctaccg atcatacaaa agatcatcat catggatagc
     1321 aagaccgact accagggctt ccaaagcatg tacaccttcg tgacttccca tttgccaccc
     1381 ggcttcaacg agtacgactt cgtgcccgag agcttcgacc gggacaaaac catcgccctg
     1441 atcatgaaca gtagtggcag taccggattg cccaagggcg tagccctacc gcaccgcacc
     1501 gcttgtgtcc gattcagtca tgcccgcgac cccatcttcg gcaaccagat catccccgac
     1561 accgctatcc tcagcgtggt gccatttcac cacggcttcg gcatgttcac cacgctgggc
     1621 tacttgatct gcggctttcg ggtcgtgctc atgtaccgct tcgaggagga gctattcttg
     1681 cgcagcttgc aagactataa gattcaatct gccctgctgg tgcccacact atttagcttc
     1741 ttcgctaaga gcactctcat cgacaagtac gacctaagca acttgcacga gatcgccagc
     1801 ggcggggcgc cgctcagcaa ggaggtaggt gaggccgtgg ccaaacgctt ccacctacca
     1861 ggcatccgcc agggctacgg cctgacagaa acaaccagcg ccattctgat cacccccgaa
     1921 ggggacgaca agcctggcgc agtaggcaag gtggtgccct tcttcgaggc taaggtggtg
     1981 gacttggaca ccggtaagac actgggtgtg aaccagcgcg gcgagctgtg cgtccgtggc
     2041 cccatgatca tgagcggcta cgttaacaac cccgaggcta caaacgctct catcgacaag
     2101 gacggctggc tgcacagcgg cgacatcgcc tactgggacg aggacgagca cttcttcatc
     2161 gtggaccggc tgaagagcct gatcaaatac aagggctacc aggtagcccc agccgaactg
     2221 gagagcatcc tgctgcaaca ccccaacatc ttcgacgccg gggtcgccgg cctgcccgac
     2281 gacgatgccg gcgagctgcc cgccgcagtc gtcgtgctgg aacacggtaa aaccatgacc
     2341 gagaaggaga tcgtggacta tgtggccagc caggttacaa ccgccaagaa gctgcgcggt
     2401 ggtgttgtgt tcgtggacga ggtgcctaaa ggactgaccg gcaagttgga cgcccgcaag
     2461 atccgcgaga ttctcattaa ggccaagaag ggcggcaaga tcgccgtgta ataattctag
     2521 agtcggggcg gccggccgct tcgagcagac atgataagat acattgatga gtttggacaa
     2581 accacaacta gaatgcagtg aaaaaaatgc tttatttgtg aaatttgtga tgctattgct
     2641 ttatttgtaa ccattataag ctgcaataaa caagttaaca acaacaattg cattcatttt
     2701 atgtttcagg ttcaggggga ggtgtgggag gttttttaaa gcaagtaaaa cctctacaaa
     2761 tgtggtaaaa tcgataagga tccgtttgcg tattgggcgc tcttccgctg atctgcgcag
     2821 caccatggcc tgaaataacc tctgaaagag gaacttggtt agctaccttc tgaggcggaa
     2881 agaaccagct gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc tccccagcag
     2941 gcagaagtat gcaaagcatg catctcaatt agtcagcaac caggtgtgga aagtccccag
     3001 gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accatagtcc
     3061 cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc
     3121 atggctgact aatttttttt atttatgcag aggccgaggc cgcctctgcc tctgagctat
     3181 tccagaagta gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag ctcgattctt
     3241 ctgacactag cgccaccatg atcgaacaag acggcctcca tgctggcagt cccgcagctt
     3301 gggtcgaacg cttgttcggg tacgactggg cccagcagac catcggatgt agcgatgcgg
     3361 ccgtgttccg tctaagcgct caaggccggc ccgtgctgtt cgtgaagacc gacctgagcg
     3421 gcgccctgaa cgagcttcaa gacgaggctg cccgcctgag ctggctggcc accaccggcg
     3481 taccctgcgc cgctgtgttg gatgttgtga ccgaagccgg ccgggactgg ctgctgctgg
     3541 gcgaggtccc tggccaggat ctgctgagca gccaccttgc ccccgctgag aaggtttcta
     3601 tcatggccga tgcaatgcgg cgcctgcaca ccctggaccc cgctacctgc cccttcgacc
     3661 accaggctaa gcatcggatc gagcgtgctc ggacccgcat ggaggccggc ctggtggacc
     3721 aggacgacct ggacgaggag catcagggcc tggcccccgc tgaactgttc gcccgactga
     3781 aagcccgcat gccggacggt gaggacctgg ttgtcacaca cggagatgcc tgcctcccta
     3841 acatcatggt cgagaatggc cgcttctccg gcttcatcga ctgcggtcgc ctaggagttg
     3901 ccgaccgcta ccaggacatc gccctggcca cccgcgacat cgctgaggag cttggcggcg
     3961 agtgggccga ccgcttctta gtcttgtacg gcatcgcagc tcccgacagc cagcgcatcg
     4021 ccttctaccg cttgctcgac gagttctttt aatgatctag aaccggtcat ggccgcaata
     4081 aaatatcttt attttcatta catctgtgtg ttggtttttt gtgtgttcga actagatgct
     4141 gtcgaccgat gcccttgaga gccttcaacc cagtcagctc cttccggtgg gcgcggggca
     4201 tgactatcgt cgccgcactt atgactgtct tctttatcat gcaactcgta ggacaggtgc
     4261 cggcagcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg
     4321 cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac
     4381 gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg
     4441 ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca
     4501 agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc
     4561 tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc
     4621 ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag
     4681 gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc
     4741 ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca
     4801 gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg
     4861 aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg
     4921 aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct
     4981 ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa
     5041 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa
     5101 gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa
     5161 tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag cggccgcaaa
     5221 tgctaaacca ctgcagtggt taccagtgct tgatcagtga ggcaccgatc tcagcgatct
     5281 gcctatttcg ttcgtccata gtggcctgac tccccgtcgt gtagatcact acgattcgtg
     5341 agggcttacc atcaggcccc agcgcagcaa tgatgccgcg agagccgcgt tcaccggccc
     5401 ccgatttgtc agcaatgaac cagccagcag ggagggccga gcgaagaagt ggtcctgcta
     5461 ctttgtccgc ctccatccag tctatgagct gctgtcgtga tgctagagta agaagttcgc
     5521 cagtgagtag tttccgaaga gttgtggcca ttgctactgg catcgtggta tcacgctcgt
     5581 cgttcggtat ggcttcgttc aactctggtt cccagcggtc aagccgggtc acatgatcac
     5641 ccatattatg aagaaatgca gtcagctcct tagggcctcc gatcgttgtc agaagtaagt
     5701 tggccgcggt gttgtcgctc atggtaatgg cagcactaca caattctctt accgtcatgc
     5761 catccgtaag atgcttttcc gtgaccggcg agtactcaac caagtcgttt tgtgagtagt
     5821 gtatacggcg accaagctgc tcttgcccgg cgtctatacg ggacaacacc gcgccacata
     5881 gcagtacttt gaaagtgctc atcatcggga atcgttcttc ggggcggaaa gactcaagga
     5941 tcttgccgct attgagatcc agttcgatat agcccactct tgcacccagt tgatcttcag
     6001 catcttttac tttcaccagc gtttcggggt gtgcaaaaac aggcaagcaa aatgccgcaa
     6061 agaagggaat gagtgcgaca cgaaaatgtt ggatgctcat actcgtcctt tttcaatatt
     6121 attgaagcat ttatcagggt tactagtacg tctctcaagg ataagtaagt aatattaagg
     6181 tacgggaggt attggacagg ccgcaataaa atatctttat tttcattaca tctgtgtgtt
     6241 ggttttttgt gtgaatcgat agtactaaca tacgctctcc atcaaaacaa aacgaaacaa
     6301 aacaaactag caaaataggc tgtccccagt gcaagtgcag gtgccagaac atttctct
//

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