pMETαB

价格:1500元

联系方式:I47-825O-882O

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pMETalpha B载体基本信息

出品公司: Invitrogen
载体名称: pMETalpha B, pMETα B, pMETalphaB, pMETαB
质粒类型: 毕赤酵母蛋白表达载体(methanoloica酵母)
表达水平: 高拷贝
诱导方法: 甲醇诱导
启动子: AUG1
克隆方法: 多克隆位点,限制性内切酶
载体大小: 8048 bp
5' 测序引物及序列: AUG1-F: 5´-CAATTTACATCTTTATTTATTAACG-3´;  alpha-Factor-F: 5´-TACTATTGCCAGCATTGCTGC-3´
3' 测序引物及序列: AUG1-R: 5´-GAAGAGAAAAACATTAGTTGGC-3´
载体标签: C-V5, C-His,N-alpha分泌因子
载体抗性: 氨苄
筛选标记: ADE2
备注: 含有alpha factor酵母分泌信号肽,利用甲醇诱导蛋白在methanoloica酵母中分泌表达蛋白。
产品目录号: K1780-01
稳定性: 稳定 Stable
组成型/诱导型: 诱导型
病毒/非病毒: 非病毒

pMETalpha B载体质粒图谱和多克隆位点信息

pMETalpha B载体图谱

pMETalpha B多克隆位点

pMETalpha B载体简介

pMETalpha B载体序列

LOCUS       pMET\alpha\B            8048 bp    DNA     circular      1-AUG-2013
DEFINITION  pMET alpha B.
SOURCE      
  ORGANISM  other sequences; artificial sequences; vectors.
COMMENT     http://www.biofeng.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTNAME|pMET alpha B|
COMMENT     VNTOAUTHORNAME|biofeng.com|
FEATURES             Location/Qualifiers
     misc_feature    1106..1130
                     /vntifkey="21"
                     /label=AUG1_fwd_primer
     misc_feature    complement(1667..1688)
                     /vntifkey="21"
                     /label=AUG1_rev_primer
     CDS             3090..3665
                     /vntifkey="4"
                     /label=ORF\frame\1
     CDS             3671..4804
                     /vntifkey="4"
                     /label=ORF\frame\3
     CDS             4968..5966
                     /vntifkey="4"
                     /label=ORF\frame\1
     gene            complement(6779..7639)
                     /gene="Ampicillin"
                     /vntifkey="60"
     CDS             complement(6779..7639)
                     /vntifkey="4"
                     /label=ORF\frame\2
     promoter        complement(7681..7709)
                     /vntifkey="30"
                     /label=AmpR_promoter
     sig_peptide     1197..1463
                     /vntifkey="94"
                     /label=Alpha\factor\secretion\signal
BASE COUNT     2362 a      1741 c      1569 g      2376 t 
ORIGIN
        1 ggcgcgcctt aattaaggcc ggcccctgca gggggccccg agatggtaca tacttaaaag 
       61 ctgccatatt gaggaacttc aaagttttat ctgtttttag aattaaaaga cgattgttgt 
      121 aacaaaacgt tgtgcctaca taaactcaaa ttaatggaaa tagcctgttt tgaaaaatac 
      181 accttcttaa gtactgacaa agttttgtta aatgactatc gaacaagcca tgaaatagca 
      241 catttctgcc agtcactttt aacactttcc tgcttgctgg ttgactctcc tcatacaaac 
      301 acccaaaagg gaaactttca gtgtggggac acttgacatc tcacatgcac cccagattaa 
      361 tttccccaga cgatgcggag acaagacaaa acaacccttt gtcctgctct tttctttctc 
      421 acaccgcgtg ggtgtgtgcg caggcaggca ggcaggcagc gggctgcctg ccatctctaa 
      481 tcgctgctcc tcccccctgg cttcaaataa cagcctgctg ctatctgtga ccagattggg 
      541 acacccccct cccctccgaa tgatccatca ccttttgtcg tactccgaca atgatccttc 
      601 cctgtcatct tctggcaatc agctccttca ataattaaat caaataagca taaatagtaa 
      661 aatcgcatac aaacgtcatg aaaagtttta tctctatggc caacggatag tctatctgct 
      721 taattccatc cactttggga accgttctct ctttacccca gattctcaaa gctaatatct 
      781 gccccttgtc tattgtcctt tctccgtgta caagcggagc ttttgcctcc catcctcttg 
      841 ctttgtttcg gttatttttt ttttcttttg aaactcttgg tcaaatcaaa tcaaacaaaa 
      901 ccaaaccttc tattccatca gatcaacctt gttcaacatt ctataaatcg atataaatat 
      961 aaccttatcc ctcccttgtt ttttaccaat taatcaatct tcaaatttca aatattttct 
     1021 acttgcttta ttactcagta ttaacatttg tttaaaccaa ctataacttt taactggctt 
     1081 tagaagtttt atttaacatc agtttcaatt tacatcttta tttattaacg aaatctttac 
     1141 gaattaactc aatcaaaact tttacgaaaa aaaaatctta ctattaagag ctcaaaatga 
     1201 gatttccttc aatttttact gcagttttat tcgcagcatc ctccgcatta gctgctccag 
     1261 tcaacactac aacagaagat gaaacggcac aaattccggc tgaagctgtc atcggttact 
     1321 cagatttaga aggggatttc gatgttgctg ttttgccatt ttccaacagc acaaataacg 
     1381 ggttattgtt tataaatact actattgcca gcattgctgc taaagaagaa ggggtatctc 
     1441 tcgagaaaag agaggctgaa gctgcaggaa ttcacgtggc ccagccggcc gtggatccac 
     1501 gcgtcgtcga cccgcggcgg ccgccagctt actagtaggt aagcctatcc ctaaccctct 
     1561 cctcggtctc gattctacgc gtaccggtca tcatcaccat caccattgat ctagtataca 
     1621 attctagggc tgcctgtttg gatattttta taatttttga gagtttgcca actaatgttt 
     1681 ttctcttcta tgatatttat catgtagttg gatatctttt tttctttgaa aagtttttat 
     1741 ttagttcttt ctctcttttt tataatgttt tcagtttctt tcctttatat tgaaaatacg 
     1801 atgttctttg tctttctttt ttaaatcctc aattataatg tgtaccgttt gatcaacttg 
     1861 taaaattgtt tgtagtcctg taatcttaat tgacgattgg ttgatagttt accccagtat 
     1921 attgtgcggc tgagatatta cccgattcgg aagattgatt ttttcaacag acataaaccc 
     1981 ataggagatt tagtacagcg acatatgcta attgcctgat aatgctcact aacattttct 
     2041 ccttttaatc tggtatatgc atacaggtat gttgatatgg ccttgttgat caaagctacg 
     2101 aaagtgtaga ctatttccca aacagccccc aaccaccacc tcacacgcgc acactgcgaa 
     2161 gtgaaagcgc tcccttattt tcttctccgc tggttcaaaa caaaaaagaa tctaatctgt 
     2221 agacacacgc cccgtaaaac ccaatcttca tcatcgtaat tttcagtgac ggacgatcga 
     2281 tcaaccatat agtttgttag tgactgagct tcagtagttg acctcttgaa gaaataacgt 
     2341 cttgaacaac atactttata cctaccaaca ttcgccatca acaacagtgt gacactgaca 
     2401 taactttaca tccattcatc gacaaaaaaa tgtctctagt tcggattcat gcttatgggt 
     2461 tagctatttc gcccaatgtg tccatctgac attactattt tgcattttaa tttaattaga 
     2521 acttgactag cgcactacca gcatatcatc tcatttccgt aaataccaaa tgtattatat 
     2581 attgaaagct tttgaccagg ttattataaa agaaacttca tgctcgaaaa agatcatttc 
     2641 gaaaagttgc ctagtttcat gaaattttaa agcagtttat ataaatttta ccttttgatg 
     2701 cggaattgac tttttcttga ataatacata acttttctta aaagaatcaa agacagataa 
     2761 aatttaagag atattaaata ttagtgagaa gccgagaatt ttgtaacacc aacataacac 
     2821 tgacatcttt aacaactttt aattatgata catttcttac gtcatgattg attattacag 
     2881 ctatgctgac aaatgactct tgttgcatgg ctacgaaccg ggtaatacta agtgattgac 
     2941 tcttgctgac cttttattaa gaactaaatg gacaatatta tggagcattt catgtataaa 
     3001 ttggtgcgta aaatcgttgg atctctcttc taagtacatc ctactataac aatcaagaaa 
     3061 aacaagaaaa tcggacaaaa caatcaagta tggattctag aacagttggt atattaggag 
     3121 ggggacaatt gggacgtatg attgttgagg cagcaaacag gctcaacatt aagacggtaa 
     3181 tactagatgc tgaaaattct cctgccaaac aaataagcaa ctccaatgac cacgttaatg 
     3241 gctccttttc caatcctctt gatatcgaaa aactagctga aaaatgtgat gtgctaacga 
     3301 ttgagattga gcatgttgat gttcctacac taaagaatct tcaagtaaaa catcccaaat 
     3361 taaaaattta cccttctcca gaaacaatca gattgataca agacaaatat attcaaaaag 
     3421 agcatttaat caaaaatggt atagcagtta cccaaagtgt tcctgtggaa caagccagtg 
     3481 agacgtccct attgaatgtt ggaagagatt tgggttttcc attcgtcttg aagtcgagga 
     3541 ctttggcata cgatggaaga ggtaacttcg ttgtaaagaa taaggaaatg attccggaag 
     3601 ctttggaagt actgaaggat cgtcctttgt cgccgaaaaa tgggcaccat ttactaaaga 
     3661 attagcagtc atgattgtga gatctgttaa cggtttagtg ttttcttacc caattgtaga 
     3721 gactatccac aaggacaata tttgtgactt atgttatgcg cctgctagag ttccggactc 
     3781 cgttcaactt aaggcgaagt tgttggcaga aaatgcaatc aaatcttttc ccggttgtgg 
     3841 tatatttggt gtggaaatgt tctatttaga aacaggggaa ttgcttatta acgaaattgc 
     3901 cccaaggcct cacaactctg gacattatac cattgatgct tgcgtcactt ctcaatttga 
     3961 agctcatttg agatcaatat tggatttgcc aatgccaaag aatttcacat ctttctccac 
     4021 cattacaacg aacgccatta tgctaaatgt tcttggagac aaacatacaa aagataaaga 
     4081 gctagaaact tgcgaaagag cattggcgac tccaggttcc tcagtgtact tatatggaaa 
     4141 agagtctaga cctaacagaa aagtaggtca cataaatatt attgcctcca gtatggcgga 
     4201 atgtgaacaa aggctgaact acattacagg tagaactgat attccaatca aaatctctgt 
     4261 cgctcaaaag ttggacttgg aagcaatggt caaaccattg gttggaatca tcatgggatc 
     4321 agactctgac ttgccggtaa tgtctgccgc atgtgcggtt ttaaaagatt ttggcgttcc 
     4381 atttgaagtg acaatagtct ctgctcatag aactccacat aggatgtcag catatgctat 
     4441 ttccgcaagc aagcgtggaa ttaaaacaat tatcgctgga gctggtgggg ctgctcactt 
     4501 gccaggtatg gtggctgcaa tgacaccact tcctgtcatc ggtgtgcccg taaaaggttc 
     4561 ttgtctagat ggagtagatt ctttacattc aattgtgcaa atgcctagag gtgttccagt 
     4621 agctaccgtc gctattaata atagtacgaa cgctgcgctg ttggctgtca gactgcttgg 
     4681 cgcttatgat tcaagttata caacgaaaat ggaacagttt ttattaaagc aagaagaaga 
     4741 agttcttgtc aaagcacaaa agttagaaac tgtcggttac gaagcttatc tagaaaacaa 
     4801 gtaatatata agtttattga tatacttgta cagcaaataa ttataaaatg atatacctat 
     4861 tttttaggct ttgttatgat tacatcaaat gtggacttca tacatagaaa tcaacgctta 
     4921 caggtgtcct tttttaagaa tttcatacat aagatctact agaatcaatg ttaaacctgt 
     4981 tatcattaga acagccatct ccagaaggtt ctatccaagt gaaaactttg gtcttcaagc 
     5041 caaagactgc taaaacacaa accccagtcc caattattgt tgttgctgaa acttctactt 
     5101 ctactccggg tggtgttata gccaaagctt cgggcactaa agagccaaga ttagcaaacg 
     5161 atgaattgac caaccaattc tttggttctc ctgctaaaga gttttctatt gccagcttat 
     5221 caaacgaaca caaagacggt aaagtcaaga tcattattga tgctgggatc aaagatgatg 
     5281 actcgactaa ttacgttatt tccaacactg ttgctaagtt agttttaacc ggtaagcaat 
     5341 tattaggttt cttatccaac tacgaaccaa ccatcgttga ttttactgct gaagctgcaa 
     5401 ccgctgcccc aactccagtt gttaagaaag aacctgctgc atctggtaag cttgatgatg 
     5461 ccaaattaat tggtgttact gctgacaagg aaaaagattt ctccaactgg tactcccaaa 
     5521 ttctaatgaa gggtgaaatg ttagactact acgatgtctc tggttgttac attatgagac 
     5581 cagcttctta cacaatttgg gaaaagattc aatcttggtt cgattcgaag attaaagaag 
     5641 atggtgttac taatgcttat ttcccaatgt ttgtctcatc caaggtttta gaaaaggaaa 
     5701 aggaccacat cgatggtttc gctcctgaag ttgcttgggt taccagagct ggtaaatctg 
     5761 aattagaaga accaattgct attagaccaa cttctgaaac tgttatgggt accctgcagg 
     5821 ggccggcctt aattaaggcg cgcccttcct cgctcactga ctcgctgcgc tcggtcgttc 
     5881 ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag 
     5941 gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa 
     6001 aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc 
     6061 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc 
     6121 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg 
     6181 cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt 
     6241 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc 
     6301 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc 
     6361 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag 
     6421 agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg 
     6481 ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa 
     6541 ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag 
     6601 gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact 
     6661 cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa 
     6721 attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt 
     6781 accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag 
     6841 ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca 
     6901 gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc 
     6961 agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt 
     7021 ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg 
     7081 ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca 
     7141 gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg 
     7201 ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca 
     7261 tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg 
     7321 tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct 
     7381 cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca 
     7441 tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca 
     7501 gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg 
     7561 tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac 
     7621 ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt 
     7681 attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc 
     7741 cgcgcacatt tccccgaaaa gtgccacctg tatgcggtgt gaaataccgc acagatgcgt 
     7801 aaggagaaaa taccgcatca ggaaattgta agcgttaata ttttgttaaa attcgcgtta 
     7861 aatttttgtt aaatcagctc attttttaac caataggccg aaatcggcaa aatcccttat 
     7921 aaatcaaaag aatagaccga gatagggttg agtgttgttc cagtttggaa caagagtcca 
     7981 ctattaaaga acgtggactc caacgtcaaa gggcgaaaaa ccgtctatca agggcgatgg 
     8041 cccactac 
//

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