| 出品公司: | -- |
|---|---|
| 载体名称: | pcDNA6-Flag-N/Myc-His |
| 质粒类型: | 哺乳动物表达载体 |
| 高拷贝/低拷贝: | -- |
| 克隆方法: | -- |
| 启动子: | CMV |
| 载体大小: | 5151 bp |
| 5' 测序引物及序列: | -- |
| 3' 测序引物及序列: | -- |
| 载体标签: | N-Flag, C-Myc, C-His |
| 载体抗性: | 博莱霉素 |
| 筛选标记: | 博莱霉素 |
| 克隆菌株: | -- |
| 宿主细胞（系）: | - |
| 备注: | -- |
| 稳定性: | -- |
| 组成型/诱导型: | -- |
| 病毒/非病毒: | -- |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pcDNA6-Flag-N/Myc-His 5151 bp DNA circular SYN 12-JUL-2023
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 5151)
AUTHORS Biofeng
TITLE Direct Submission
JOURNAL Exported Jul 12, 2023 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..5151
/mol_type="other DNA"
/organism="synthetic DNA construct"
enhancer 235..614
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 615..818
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
promoter 863..881
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
regulatory 908..917
/label=Kozak sequence
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
/regulatory_class="other"
CDS 917..940
/codon_start=1
/product="FLAG(R) epitope tag, followed by an enterokinase
cleavage site"
/label=FLAG
/translation="DYKDDDDK"
CDS 1022..1051
/codon_start=1
/product="Myc (human c-Myc proto-oncogene) epitope tag"
/label=Myc
/translation="EQKLISEEDL"
CDS 1067..1084
/codon_start=1
/product="6xHis affinity tag"
/label=6xHis
/translation="HHHHHH"
polyA_signal 1113..1337
/label=bGH poly(A) signal
/note="bovine growth hormone polyadenylation signal"
rep_origin 1383..1811
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 1825..2154
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
rep_origin 2005..2140
/label=SV40 ori
/note="SV40 origin of replication"
promoter 2202..2249
/label=EM7 promoter
/note="synthetic bacterial promoter "
CDS 2268..2666
/codon_start=1
/gene="Aspergillus terreus BSD"
/product="blasticidin S deaminase"
/label=BSD
/note="confers resistance to blasticidin"
/translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
KAIVKDSDGQPTAVGIRELLPSGYVWEG"
polyA_signal 2824..2945
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(2994..3010)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
protein_bind 3018..3034
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(3042..3072)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 3087..3108
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
rep_origin complement(3396..3984)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(4155..5015)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
promoter complement(5016..5120)
/gene="bla"
/label=AmpR promoter
ORIGIN
1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagt
901 taagcttgcc accatggatt acaaggatga cgacgataag ggatccacta gtccagtgtg
961 gtggaattct gcagatatcc agcacagtgg cggccgctcg agtctagagg gcccgcggtt
1021 cgaacaaaaa ctcatctcag aagaggatct gaatatgcat accggtcatc atcaccatca
1081 ccattgagtt taaacccgct gatcagcctc gactgtgcct tctagttgcc agccatctgt
1141 tgtttgcccc tcccccgtgc cttccttgac cctggaaggt gccactccca ctgtcctttc
1201 ctaataaaat gaggaaattg catcgcattg tctgagtagg tgtcattcta ttctgggggg
1261 tggggtgggg caggacagca agggggagga ttgggaagac aatagcaggc atgctgggga
1321 tgcggtgggc tctatggctt ctgaggcgga aagaaccagc tggggctcta gggggtatcc
1381 ccacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac
1441 cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc
1501 cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt
1561 tagtgcttta cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg
1621 gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag
1681 tggactcttg ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt
1741 ataagggatt ttgccgattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt
1801 taacgcgaat taattctgtg gaatgtgtgt cagttagggt gtggaaagtc cccaggctcc
1861 ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccag gtgtggaaag
1921 tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc
1981 atagtcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc cgcccattct
2041 ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc ctctgcctct
2101 gagctattcc agaagtagtg aggaggcttt tttggaggcc taggcttttg caaaaagctc
2161 ccgggagctt gtatatccat tttcggatct gatcagcacg tgttgacaat taatcatcgg
2221 catagtatat cggcatagta taatacgaca aggtgaggaa ctaaaccatg gccaagcctt
2281 tgtctcaaga agaatccacc ctcattgaaa gagcaacggc tacaatcaac agcatcccca
2341 tctctgaaga ctacagcgtc gccagcgcag ctctctctag cgacggccgc atcttcactg
2401 gtgtcaatgt atatcatttt actgggggac cttgtgcaga actcgtggtg ctgggcactg
2461 ctgctgctgc ggcagctggc aacctgactt gtatcgtcgc gatcggaaat gagaacaggg
2521 gcatcttgag cccctgcgga cggtgccgac aggtgcttct cgatctgcat cctgggatca
2581 aagccatagt gaaggacagt gatggacagc cgacggcagt tgggattcgt gaattgctgc
2641 cctctggtta tgtgtgggag ggctaagcac ttcgtggccg aggagcagga ctgacacgtg
2701 ctacgagatt tcgattccac cgccgccttc tatgaaaggt tgggcttcgg aatcgttttc
2761 cgggacgccg gctggatgat cctccagcgc ggggatctca tgctggagtt cttcgcccac
2821 cccaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc
2881 acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta
2941 tcttatcatg tctgtatacc gtcgacctct agctagagct tggcgtaatc atggtcatag
3001 ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc
3061 ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc
3121 tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa
3181 cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg
3241 ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg
3301 ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag
3361 gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac
3421 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga
3481 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt
3541 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc
3601 tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc
3661 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta
3721 agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat
3781 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca
3841 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct
3901 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt
3961 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct
4021 cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc
4081 acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa
4141 acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta
4201 tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc
4261 ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat
4321 ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta
4381 tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt
4441 aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt
4501 ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg
4561 ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc
4621 gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc
4681 gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg
4741 cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga
4801 actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta
4861 ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct
4921 tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag
4981 ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca atattattga
5041 agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat
5101 aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt c
//
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