| 出品公司: | Takara |
|---|---|
| 载体名称: | pPTRII |
| 质粒类型: | 曲霉表达质粒，大肠杆菌-曲霉穿梭质粒，曲霉表达载体 |
| 高拷贝/低拷贝: | 高拷贝 |
| 克隆方法: | 限制性内切酶，多克隆位点 |
| 启动子: | Lac |
| 载体大小: | 10031 bp |
| 5’测序引物: | M13-R: CAGGAAACAGCTATGAC |
| 3’测序引物: | M13-F: TGTAAAACGACGGCCAGT |
| 载体抗性: | 氨苄青霉素 |
| 克隆菌株: | DH5 alpha |
| 宿主细胞（系）: | 曲霉菌 |
| 产品目录号: | 3622 |
| 稳定性: | 瞬表达 |
| 组成型/诱导型: | 组成型 |
| 病毒/非病毒: | 非病毒 |
| 相关载体: | pPTRI |
| 载体说明书: | pPTRII载体说明书 |
| 备注: | pPTRII载体是曲霉表达载体，能够在曲霉菌中进行自我复制。pPTRII载体可以用于多种曲霉菌（Aspergillus species），例如A. oryzae, A . niger, A. terreus and A . fumigatus。pPTRI载体在曲霉菌中的筛选抗性是Pyrithiamine，中文名是抗硫胺素, 也叫吡啶硫铵。 本载体在构建质粒时，可以利用蓝白斑筛选进行阳性插入克隆验证。 |
买家导航
pPTRII载体 pPTR II质粒基本信息
pPTRII质粒图谱 pPTR II载体图谱和pPTRII载体序列 pPTR II质粒序列多克隆位点信息
pPTRII质粒 pPTR II载体简介
pPTR II是一可以在曲霉菌中可以自主复制的表达载体质粒。该载体具有氨苄西林抗性基因 Ampr作为大肠杆菌筛选抗性。
在曲霉中，筛选基因是抗硫胺素（Pyrithiamine） ，抗性基因为ptr A基因。本质粒具有多克隆位点，包含 Hind III，Pst I，Sma I和Kpn I。
在载体lac Z区域，任何DNA插入到这个区域 使用含有IPTG和X-Gal的平板可以很容易地进行蓝白斑筛选阳性克隆载体。
pPTRII质粒序列 pPTR II载体序列
LOCUS pTR II Vector 10031 bp DNA circular SYN 23-JUL-2021
COMMENT Created by Takara Bio Inc. http://www.biofeng.com
COMMENT Cat. No. 3622
FEATURES Location/Qualifiers
gene complement(2943..4950)
/label="ptrA"
/note="pyrithiamine resistance gene useful as a dominant selectable marker in
Aspergillus"
CDS 4968..5828
/label="AmpR"
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
rep_origin 5999..6587
/direction=RIGHT
/label="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
promoter 6911..6941
/label="lac promoter"
/note="promoter for the E. coli lac operon"
protein_bind 6949..6965
/label="lac operator"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
primer_bind 6973..6989
/label="M13 rev"
/note="common sequencing primer, one of multiple similar
variants"
CDS 6985..7311
/label="lacZ-alpha"
misc_feature 7002..7058
/label="MCS"
/note="pUC18/19 multiple cloning site"
primer_bind complement(7059..7075)
/label="M13 fwd"
/note="common sequencing primer, one of multiple similar
variants"
rep_origin join(7416..10031,1..2633)
/label="AMA1"
/note="replication origin from Aspergillus nidulans
(Aleksenko and Clutterbuck, 1996)"
ORIGIN
1 ctgcagattt tgacctagtt aacaaggcac atcatgctat cacttccaac actcttgctg
61 agcgcgttct gccttgcctt tctggttcta cgaagtatcc agtcccgcct gcaacgccgc
121 agaaacgcca aaaggtgggg ttgtcaaccg ccagctagag ctccatcggg attctttggc
181 attccgccgt tcctcggtgg tcgcagataa ctccctaaat tccctgagct aactctaagt
241 cgaccatgcc gtttatggtt agcgcctccc aaaaaggaat ggccgactta gagttacctc
301 ttgaccgact ttttctttcc tcccccttac atttcgttac cacaacacat tcctatatcc
361 aaactccagg tacataacta gtcgaaatct cttttaaatc tagtcaagaa ctagatactt
421 aaccttcata ctgcttagag atagcctttg aagctcttat ttcgctcttt gtctcactct
481 caccttcctc ctcctcctcc aatccttttt gctcgggact agtccaagac tagtcaacga
541 ttagtatgcc gagcattcgc gataacgatc tccgaaagtc cccagagtac tgtcactatc
601 tcgaggcagt taaggacggg gagcttacgc tgccggattt caagatagta agccgacccg
661 actagcttta agcctagtta ctgactagtc cacaggacga caatggcgtg cctgatatcc
721 atccatatga agtctactgc cgagtgaagg gatgcctcaa gcgtacagtg agtctactgc
781 tatactagtt tctgactagt tattaactag ttgacaggtt ccctctgcca acagaaacat
841 attggtcaag cacttgaagg acaagaactc ccacggcatg gagtttacat tgcacaatgg
901 acctcccact atgaaggaac tgatggaggc caaaggcaag tcctatctag attacttggt
961 gactagtccc tgactagtca agtagcatgg tatgaaggct tgtttgaagg cactgttctc
1021 ccaaccccga ctcctaccaa gaagcgcaag cgagctgcgt aagtttctgt gagtctaact
1081 agtgtattag ctaatatata gcagcaccaa gtccaaggac cacaatacta agggtgtcga
1141 gaattcgtga gtttcttctc ccatttcaac tagtccttga ctagtcacta actactttgc
1201 agcaacgagg gtgaagctgg aaatgatcag gacaatggcg agggcccgta agtacagcca
1261 ttcaatgcag actagttgct aactaatctg tgactagttc aagtggtccg tacgccgtgc
1321 atacccctgt gactggtagg aatttgagca agcctgtctt gccgcgcgat gagaaaggaa
1381 aggcaagtta cattcagccc cgtacctagg atcagtgcta atttataacc tctagccact
1441 ctttatgcag atccgccgtg agggtagcaa ggcagctaaa tcagctggtg agaaaggaac
1501 cataccctgc aagacctgtc gcaacgcaaa gggcaaaggt aagctatcca agctagtttg
1561 ggactagatt ctaactagtc tcagcaccgt gtggttcaaa gccatattgc gagttttggc
1621 gctttttctc atcgattgac gaggcaaagg gagcgagtat gcaacctcgt aagtcagact
1681 cagacaaaga ccaactagtt attgaccagt cttctagaag gctctgttgt ggatcttgag
1741 gccctggaga gttcctccaa caatccggag acaagcaagt cctcgtcgga ctagtcacta
1801 actagactct aactagttgc agacatggat aatgcaaaag agacaagcaa tgaagaaagt
1861 ggtaagacat ttctcctttg tggttctgga ctagtctttg actagtcaca gtcttaaaca
1921 aggaaaatga gcatgaaaat gaggaggaaa aggctgctga gcccgaggaa gtgcagggtg
1981 atggcagaca tggtaggtta ataccttgtt agttattgct agtcactgac tagtcaataa
2041 ctagtctctg aacaccttgc aatcactccg tttgcgcagc tgaacagtgg tgaggataat
2101 agtagtaagt tattctagct tcagagttat aggagactag atactaacta gtattagttg
2161 caactaacct ggatctcaga gactttggcc tcaatctaga atctatctag ttgtcaacta
2221 gactgtggta tcattgtctt ttattttcct agtcctggaa ctagcttcta actagtctcc
2281 ctaatatgtg gctgtcttgt tttttttttt tgtttcccta cccggatatc tagtcccctt
2341 ctaggttctg ttaacctctc gggctctgat ttagtttaac gcaaacctga gattagtttc
2401 taactagtct ctaggttttc tatccacctt taattgtaat aataaataca agcaacgttt
2461 atacgtcaaa agcatttata aacttttacc ctaaagtagc ttgcttgtgt gtttagttta
2521 taattagtct cttattaatt tgatgtaggt aagcccgcca caaatatata tttttaacaa
2581 gataccgtgg aaaaacttcg tgctatcaca aaacagtata caaaaaataa gctggctttc
2641 cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct ttacggcacc
2701 tcgaccccaa aaaacttgat ttgggtgatg gttcacgtag tgggccatcg ccctgataga
2761 cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc ttgttccaaa
2821 ctggaacaac actcaactct atctcgggct attcttttga tttataaggg attttgccga
2881 tttcggtcta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattttaaca
2941 aaatggggtg acgatgagcc gctcttgcat ctttgtttgt attatactgt ctttcttgtt
3001 acacataatt attctagaat gccccaccgt tacatacggg acacagccat ttacatatgc
3061 atgtggatta cgagctaacg agttcattca aatctcagaa ctatcacata atcatcattc
3121 ccctatcgtc aaagaccgta agacaaatcc ggttcatgca ctgaacccat tcgggtagtg
3181 agtcatttac tcagcacact cgcgctgacg ctcgtcgaac accttcaatg cctcctcggc
3241 agccttgaca ccactgagaa ccatggcacc gaaggtaggg cccatgcggt taaagccatc
3301 aatttcagac agctccatac cgccgattat caagccctta gtaacctcgc gggtgttctt
3361 gacgatggca tcctcggccg agctcatgtc gagaccacgc atgccaccta gcttgtcgac
3421 gctgcccatg gacaccaagc gcttcgcaca gaaggcgccg aatggcccat cgtgaccagt
3481 ggtactgatg atgacaggag cgttgatagt gttggggtcc atgcaggagt gatcatcgtg
3541 gtgaagggtg accagcgtcc agttgacgac aacaccagca atctgggggt tgccgttctc
3601 ggtcggacgg gtgatcaagt cctcaacagc ggtagcattg aagagcttga cattggggaa
3661 ggagagaacc ttcgacatga gtgtcgaggt aaacagggag gcgtgcttga cgacaacgta
3721 gttggggttt gcgtcctctt cgtaaggaac acccagctcg ttcaggaaga cttccgcggg
3781 acggcgcatg accatagcag aaaagagttg gccacccaac caggcaccgc cacctattgc
3841 acgttagttc cggaaagctg agtgcaaggc aatccatcat ggactactga ccaggagaga
3901 cgctggcctc gacgatagca atcttcaggt ccggacgagc cttggccaag acgtacgcag
3961 tgctcagacc gcaggaacca gcaccaacaa tgacaacgtc actttcagcg tacttgtcca
4021 ggtcctcaaa gtaacgtctg gtcatggcac gagagacctg gctttcgcgg ataggggcga
4081 acttgaactc gtcccacttg ccaccgaaat ggtccaacag cttggtctga gaagctccct
4141 caacggggac ggtctcagaa accacgacct tacccttgag gccggtagcg gccacagtgg
4201 gttcgtagat ggcagctgga ggagacatgt ttcaagttgc aatgactatc atctgttagc
4261 cattccatca acaggaagaa cgagagaagg catgaccctt ttcgctggta ttatccagat
4321 caagttttag ccgtataatc tcagaacgaa cccagtccat cgatgccatg tccttctaga
4381 ctaggatcct agagtctagg gcccagctta gggagggcat gtgaatgcat cgatgactgg
4441 gaacgaacac cggcccacgc caaagacgtt acctaagata ccttgatcat tgtgagagtc
4501 cagccaaaag tattccatga cttccatcgt atgccctcta gagggctaat cgaggagtgt
4561 atttacattg tcggttggtt tgggaactat agaagatggt cagttattcc aatcaccaaa
4621 ggtttatcga agggaggaag acttgttcag tttcgtccga ggacttttgg aattcaaatc
4681 tgagatagag aattgtgtgg gatgagagga aaaggaagaa gatggtgggg ttcagaagga
4741 ggggttgagt taaatagcat gggttgagtc aacgtgataa gggcactata ccgtatagat
4801 cagcggcacc cgattctatc cgttcctttt gctcctcttt agctttgacc ggtgagccgg
4861 acaagaaaca agtgaaatca tcctgacatc ggcggacgat ctcctagctt ttacatttcg
4921 ttaccaatgg gatcccgtaa tcaattgccc attgaaaaag gaagagtatg agtattcaac
4981 atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc
5041 cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca
5101 tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc
5161 caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg
5221 ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac
5281 cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca
5341 taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg
5401 agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac
5461 cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg
5521 caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat
5581 taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg
5641 ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg
5701 cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc
5761 aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc
5821 attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt
5881 tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt
5941 aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt
6001 gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag
6061 cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca
6121 gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca
6181 agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg
6241 ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg
6301 cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct
6361 acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga
6421 gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc
6481 ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg
6541 agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg
6601 cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt
6661 tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc
6721 gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcccaatac
6781 gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagctggcac gacaggtttc
6841 ccgactggaa agcgggcagt gagcgcaacg caattaatgt gagttagctc actcattagg
6901 caccccaggc tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat
6961 aacaatttca cacaggaaac agctatgacc atgattacgc caagcttgca tgcctgcagg
7021 tcgactctag aggatccccg ggtaccgagc tcgaattcac tggccgtcgt tttacaacgt
7081 cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca tccccctttc
7141 gccagctggc gtaatagcga agaggcccgc accgatcgcc cttcccaaca gttgcgcagc
7201 ctgaatggcg aatggcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca
7261 caccgcatac gtcaaagcaa ccatagtacg cgccctgtag cggcgcatta agcgcggcgg
7321 gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccttagcg cccgctcctt
7381 tcgctttctt cccttccttt ctcgccacgt tcgccagctt attttttgta tactgttttg
7441 tgatagcacg aagtttttcc acggtatctt gttaaaaata tatatttgtg gcgggcttac
7501 ctacatcaaa ttaataagag actaattata aactaaacac acaagcaagc tactttaggg
7561 taaaagttta taaatgcttt tgacgtataa acgttgcttg tatttattat tacaattaaa
7621 ggtggataga aaacctagag actagttaga aactaatctc aggtttgcgt taaactaaat
7681 cagagcccga gaggttaaca gaacctagaa ggggactaga tatccgggta gggaaacaaa
7741 aaaaaaaaac aagacagcca catattaggg agactagtta gaagctagtt ccaggactag
7801 gaaaataaaa gacaatgata ccacagtcta gttgacaact agatagattc tagattgagg
7861 ccaaagtctc tgagatccag gttagttgca actaatacta gttagtatct agtctcctat
7921 aactctgaag ctagaataac ttactactat tatcctcacc actgttcagc tgcgcaaacg
7981 gagtgattgc aaggtgttca gagactagtt attgactagt cagtgactag caataactaa
8041 caaggtatta acctaccatg tctgccatca ccctgcactt cctcgggctc agcagccttt
8101 tcctcctcat tttcatgctc attttccttg tttaagactg tgactagtca aagactagtc
8161 cagaaccaca aaggagaaat gtcttaccac tttcttcatt gcttgtctct tttgcattat
8221 ccatgtctgc aactagttag agtctagtta gtgactagtc cgacgaggac ttgcttgtct
8281 ccggattgtt ggaggaactc tccagggcct caagatccac aacagagcct tctagaagac
8341 tggtcaataa ctagttggtc tttgtctgag tctgacttac gaggttgcat actcgctccc
8401 tttgcctcgt caatcgatga gaaaaagcgc caaaactcgc aatatggctt tgaaccacac
8461 ggtgctgaga ctagttagaa tctagtccca aactagcttg gatagcttac ctttgccctt
8521 tgcgttgcga caggtcttgc agggtatggt tcctttctca ccagctgatt tagctgcctt
8581 gctaccctca cggcggatct gcataaagag tggctagagg ttataaatta gcactgatcc
8641 taggtacggg gctgaatgta acttgccttt cctttctcat cgcgcggcaa gacaggcttg
8701 ctcaaattcc taccagtcac aggggtatgc acggcgtacg gaccacttga actagtcaca
8761 gattagttag caactagtct gcattgaatg gctgtactta cgggccctcg ccattgtcct
8821 gatcatttcc agcttcaccc tcgttgctgc aaagtagtta gtgactagtc aaggactagt
8881 tgaaatggga gaagaaactc acgaattctc gacaccctta gtattgtggt ccttggactt
8941 ggtgctgcta tatattagct aatacactag ttagactcac agaaacttac gcagctcgct
9001 tgcgcttctt ggtaggagtc ggggttggga gaacagtgcc ttcaaacaag ccttcatacc
9061 atgctacttg actagtcagg gactagtcac caagtaatct agataggact tgcctttggc
9121 ctccatcagt tccttcatag tgggaggtcc attgtgcaat gtaaactcca tgccgtggga
9181 gttcttgtcc ttcaagtgct tgaccaatat gtttctgttg gcagagggaa cctgtcaact
9241 agttaataac tagtcagaaa ctagtatagc agtagactca ctgtacgctt gaggcatccc
9301 ttcactcggc agtagacttc atatggatgg atatcaggca cgccattgtc gtcctgtgga
9361 ctagtcagta actaggctta aagctagtcg ggtcggctta ctatcttgaa atccggcagc
9421 gtaagctccc cgtccttaac tgcctcgaga tagtgacagt actctgggga ctttcggaga
9481 tcgttatcgc gaatgctcgg catactaatc gttgactagt cttggactag tcccgagcaa
9541 aaaggattgg aggaggagga ggaaggtgag agtgagacaa agagcgaaat aagagcttca
9601 aaggctatct ctaagcagta tgaaggttaa gtatctagtt cttgactaga tttaaaagag
9661 atttcgacta gttatgtacc tggagtttgg atataggaat gtgttgtggt aacgaaatgt
9721 aagggggagg aaagaaaaag tcggtcaaga ggtaactcta agtcggccat tcctttttgg
9781 gaggcgctaa ccataaacgg catggtcgac ttagagttag ctcagggaat ttagggagtt
9841 atctgcgacc actggactct caggctttcc attggggtac taacatagcc atcaaatgcc
9901 ggcccgtgtt taatctcctc gagatggctg ggctcacatg cgttctactc aaaaactctt
9961 atacctaggc cctctcccaa tcgtgtatgt ttggaccttc ctctctcaca ctttggataa
10021 catcaagcat a
//
