YEplac195

价格:1000元

联系方式:I47-825O-882O

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载体基本信息

出品公司: ATCC
载体名称: YEplac195
质粒类型: 酿酒酵母表达载体;2μ质粒型载体;酵母附加体型载体
高拷贝/低拷贝: 高拷贝
克隆方法: 限制性内切酶,多克隆位点
启动子: lacZ
载体大小: 5241 bp
5' 测序引物及序列: M13 Reverse:CAGGAAACAGCTATGAC
3' 测序引物及序列: M13/pUC Forward:CCCAGTCACGACGTTGTAAAACG
载体标签: --
载体抗性: 氨苄青霉素
筛选标记: URA3
表达菌株: 酿酒酵母、白色念珠菌
备注: --
产品目录号: #87589
稳定性: 稳表达
组成型/诱导型: 诱导型
病毒/非病毒: 非病毒

载体质粒图谱和多克隆位点信息

YEplac195载体图谱



YEplac195 多克隆位点

载体简介

载体序列

LOCUS       YEplac195	5241 bp 	DNA	SYN
DEFINITION  YEplac195
ACCESSION   
KEYWORDS    
SOURCE      
  ORGANISM  other sequences; artificial sequences; vectors.
FEATURES             Location/Qualifiers
     source          1..5241
                     /organism="YEplac195"
                     /mol_type="other DNA"
     promoter        142..171
                     /label="lac_promoter"
     misc_feature    185..207
                     /label="M13_pUC_rev_primer"
     promoter        206..224
                     /label="M13_reverse_primer"
     misc_feature    287..442
                     /label="lacZ_a"
     promoter        complement(290..306)
                     /label="M13_forward20_primer"
     misc_feature    complement(299..321)
                     /label="M13_pUC_fwd_primer"
     rep_origin      complement(743..1907)
                     /label="2micron2_origin"
     CDS             complement(2200..3003)
                     /label="ORF frame 1"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKEL
                     LELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVK
                     LQYSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLS
                     TGEYTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYR
                     TVDDVVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN*"
     gene            complement(2203..3003)
                     /label="URA3"
                     /gene="URA3"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKEL
                     LELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVK
                     LQYSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLS
                     TGEYTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYR
                     TVDDVVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN*"
     CDS             2492..2968
                     /label="ORF frame 2"
                     /translation="MSTVPLVYSPVDREPLHDNSANIKRPLGSFVTSSAACFKPLTIP
                     GPTTPCAFVMSAHSAILYTPAEYCNLTVLPMSANFLSSKSKKLYLADNAFSGLTVPSM
                     EKSVKISTCVFSKQILGPNASTNSSNSLVVRTSNEAHKFVCFSCMILNSLAATGLG*"
     promoter        3370..3398
                     /label="AmpR_promoter"
                     /translation="MSTVPLVYSPVDREPLHDNSANIKRPLGSFVTSSAACFKPLTIP
                     GPTTPCAFVMSAHSAILYTPAEYCNLTVLPMSANFLSSKSKKLYLADNAFSGLTVPSM
                     EKSVKISTCVFSKQILGPNASTNSSNSLVVRTSNEAHKFVCFSCMILNSLAATGLG*"
     gene            3440..4300
                     /label="Ampicillin"
                     /gene="Ampicillin"
                     /translation="MSTVPLVYSPVDREPLHDNSANIKRPLGSFVTSSAACFKPLTIP
                     GPTTPCAFVMSAHSAILYTPAEYCNLTVLPMSANFLSSKSKKLYLADNAFSGLTVPSM
                     EKSVKISTCVFSKQILGPNASTNSSNSLVVRTSNEAHKFVCFSCMILNSLAATGLG*"
     CDS             3440..4300
                     /label="ORF frame 2"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
     rep_origin      4455..5074
                     /label="pBR322_origin"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
ORIGIN
    1 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
   61 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct
  121 cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat
  181 tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg ccaagcttgc
  241 atgcctgcag gtcgactcta gaggatcccc gggtaccgag ctcgaattca ctggccgtcg
  301 ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc cttgcagcac
  361 atcccccttt cgccagctgg cgtaatagcg aagaggcccg caccgatcgc ccttcccaac
  421 agttgcgcag cctgaatggc gaatggcgcc tgatgcggta ttttctcctt acgcatctgt
  481 gcggtatttc acaccgcata tatcggatcg tacttgttac ccatcattga attttgaaca
  541 tccgaacctg ggagttttcc ctgaaacaga tagtatattt gaacctgtat aataatatat
  601 agtctagcgc tttacggaag acaatgtatg tatttcggtt cctggagaaa ctattgcatc
  661 tattgcatag gtaatcttgc acgtcgcatc cccggttcat tttctgcgtt tccatcttgc
  721 acttcaatag catatctttg ttaacgaagc atctgtgctt cattttgtag aacaaaaatg
  781 caacgcgaga gcgctaattt ttcaaacaaa gaatctgagc tgcattttta cagaacagaa
  841 atgcaacgcg aaagcgctat tttaccaacg aagaatctgt gcttcatttt tgtaaaacaa
  901 aaatgcaacg cgagagcgct aatttttcaa acaaagaatc tgagctgcat ttttacagaa
  961 cagaaatgca acgcgagagc gctattttac caacaaagaa tctatacttc ttttttgttc
 1021 tacaaaaatg catcccgaga gcgctatttt tctaacaaag catcttagat tacttttttt
 1081 ctcctttgtg cgctctataa tgcagtctct tgataacttt ttgcactgta ggtccgttaa
 1141 ggttagaaga aggctacttt ggtgtctatt ttctcttcca taaaaaaagc ctgactccac
 1201 ttcccgcgtt tactgattac tagcgaagct gcgggtgcat tttttcaaga taaaggcatc
 1261 cccgattata ttctataccg atgtggattg cgcatacttt gtgaacagaa agtgatagcg
 1321 ttgatgattc ttcattggtc agaaaattat gaacggtttc ttctattttg tctctatata
 1381 ctacgtatag gaaatgttta cattttcgta ttgttttcga ttcactctat gaatagttct
 1441 tactacaatt tttttgtcta aagagtaata ctagagataa acataaaaaa tgtagaggtc
 1501 gagtttagat gcaagttcaa ggagcgaaag gtggatgggt aggttatata gggatatagc
 1561 acagagatat atagcaaaga gatacttttg agcaatgttt gtggaagcgg tattcgcaat
 1621 attttagtag ctcgttacag tccggtgcgt ttttggtttt ttgaaagtgc gtcttcagag
 1681 cgcttttggt tttcaaaagc gctctgaagt tcctatactt tctagctaga gaataggaac
 1741 ttcggaatag gaacttcaaa gcgtttccga aaacgagcgc ttccgaaaat gcaacgcgag
 1801 ctgcgcacat acagctcact gttcacgtcg cacctatatc tgcgtgttgc ctgtatatat
 1861 atatacatga gaagaacggc atagtgcgtg tttatgctta aatgcgtact tatatgcgtc
 1921 tatttatgta ggatgaaagg tagtctagta cctcctgtga tattatccca ttccatgcgg
 1981 ggtatcgtat gcttccttca gcactaccct ttagctgttc tatatgctgc cactcctcaa
 2041 ttggattagt ctcatccttc aatgctatca tttcctttga tattggatcg atccgatgat
 2101 aagctgtcaa acatgagaat tgggtaataa ctgatataat taaattgaag ctctaatttg
 2161 tgagtttagt atacatgcat ttacttataa tacagttttt tagttttgct ggccgcatct
 2221 tctcaaatat gcttcccagc ctgcttttct gtaacgttca ccctctacct tagcatccct
 2281 tccctttgca aatagtcctc ttccaacaat aataatgtca gatcctgtag agaccacatc
 2341 atccacggtt ctatactgtt gacccaatgc gtctcccttg tcatctaaac ccacaccggg
 2401 tgtcataatc aaccaatcgt aaccttcatc tcttccaccc atgtctcttt gagcaataaa
 2461 gccgataaca aaatctttgt cgctcttcgc aatgtcaaca gtacccttag tatattctcc
 2521 agtagatagg gagcccttgc atgacaattc tgctaacatc aaaaggcctc taggttcctt
 2581 tgttacttct tctgccgcct gcttcaaacc gctaacaata cctgggccca ccacaccgtg
 2641 tgcattcgta atgtctgccc attctgctat tctgtataca cccgcagagt actgcaattt
 2701 gactgtatta ccaatgtcag caaattttct gtcttcgaag agtaaaaaat tgtacttggc
 2761 ggataatgcc tttagcggct taactgtgcc ctccatggaa aaatcagtca agatatccac
 2821 atgtgttttt agtaaacaaa ttttgggacc taatgcttca actaactcca gtaattcctt
 2881 ggtggtacga acatccaatg aagcacacaa gtttgtttgc ttttcgtgca tgatattaaa
 2941 tagcttggca gcaacaggac taggatgagt agcagcacgt tccttatatg tagctttcga
 3001 catgatttat cttcgtttcc tgcatgtttt tgttctgtgc agttgggtta agaatactgg
 3061 gcaatttcat gtttcttcaa cactacatat gcgtatatat accaatctaa gtctgtgctc
 3121 cttccttcgt tcttccttct gttcggagat taccgaatca aaaaaatttc aaagaaaccg
 3181 aaatcaaaaa aaagaataaa aaaaaaatga tgaattgaat tgaaaagcta attcttgaag
 3241 acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc
 3301 ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt
 3361 ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata
 3421 atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt
 3481 tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc
 3541 tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat
 3601 ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct
 3661 atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc gccgcataca
 3721 ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg
 3781 catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa
 3841 cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg
 3901 ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga
 3961 cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg
 4021 cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt
 4081 tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg
 4141 agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc
 4201 ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca
 4261 gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc
 4321 atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat
 4381 cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc
 4441 agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg
 4501 ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct
 4561 accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct
 4621 tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct
 4681 cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg
 4741 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc
 4801 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga
 4861 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg
 4921 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta
 4981 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg
 5041 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg
 5101 ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat
 5161 taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc
 5221 agtgagcgag gaagcggaag a
//

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