| 质粒名称: | pAKlux2 |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 14080 |
| 存储实验室: | Attila Karsi |
|
载体骨架基本信息 |
|
| 载体名称: | pBBR1MCS4 |
| 载体出品公司: | -- |
| 载体抗性: | 氨苄青霉素 |
| 空载体大小: | 4982 bp |
| 完整质粒大小: | -- |
| 载体修饰: | -- |
| 载体类型: | 细菌表达 |
| 筛选标记: | -- |
| 高拷贝/低拷贝: | 低拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | Bacterial luciferase,luxCDABE |
| 物种来源: | -- |
| 插入大小: | 5843 bp |
| 基因突变: | -- |
| GenBank编号 | -- |
| 启动子 | -- |
| 标签/融合蛋白: | -- |
| 克隆方法: | 限制性内切酶 |
| 5' 克隆位点: | EcoR1(未破坏) |
| 3' 克隆位点: | EcoR1(未破坏) |
| 5' 测序引物 | T3 |
| 3' 测序引物 | T7 |
|
文献引用方法 |
|
| 材料和方法部分: | pAKlux2 was a gift from Attila Karsi (Addgene plasmid # 14080 ; http://n2t.net/addgene:14080 ; RRID:Addgene_14080) |
| 参考文献部分: | Broad host range fluorescence and bioluminescence expression vectors for Gram-negative bacteria. Karsi A, Lawrence ML. Plasmid. 2007 Jan 4. ():. 10.1016/j.plasmid.2006.11.002 PubMed 17207855 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pAKlux2 10801 bp ds-DNA circular SYN 19-MAR-2019
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS pAKlux2
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 10801)
AUTHORS Karsi A, Lawrence ML
TITLE Broad host range fluorescence and bioluminescence expression vectors
for Gram-negative bacteria.
JOURNAL Plasmid. 2007 Jan 4. ():.
PUBMED 17207855
REFERENCE 2 (bases 1 to 10801)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Mar 19, 2019 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..10801
/organism="synthetic DNA construct"
/mol_type="other DNA"
protein_bind 229..250
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
promoter 265..295
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 303..319
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
primer_bind 308..330
/label=M13/pUC Reverse
/note="In lacZ gene"
primer_bind 327..343
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind 327..343
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind 362..382
/label=T3
/note="T3 promoter, forward primer"
promoter 364..382
/label=T3 promoter
/note="promoter for bacteriophage T3 RNA polymerase"
primer_bind 411..427
/label=pBluescriptKS
/note="For pBluescript vector"
primer_bind 412..428
/label=KS primer
/note="common sequencing primer, one of multiple similar
variants"
CDS 466..1908
/codon_start=1
/gene="luxC"
/product="LuxC fatty acid reductase"
/label=LuxC
/note="from the Photorhabdus luminescens bioluminescence
operon"
/translation="MTKKISFIINGQVEIFPEGDDLVQSINFGDNSVYLPILNDSHVKN
IIDCNGNNELRLHNIVNFLYTVGQRWKNEEYSRRRTYIRDLKKYMGYSEEMAKLEANWI
SMILCSKGGLYDVVENELGSRHIMDEWLPQDESYVRAFPKGKSVHLLAGNVPLSGIMSI
LRAILTKNQCIIKTSSTDPFTANALALSFIDVDPNHPITRSLSVIYWPHQGDTSLAKEI
MQHADVIVAWGGPDAINWAVEHAPSYADVIKFGSKKSLCIIDNPVDLTSAATGAAHDVC
FYDQRACFSAQNIYYMGNHYEEFKLALIEKLNLYAHILPNAKKDFDEKAAYSLVQKESL
FAGLKVEVDIHQRWMIIESNAGVEFNQPLGRCVYLHHVDNIEQILPYVQKNKTQTISIF
PWESSFKYRDALALKGAERIVEAGMNNIFRVGGSHDGMRPLQRLVTYISHERPSNYTAK
DVAVEIEQTRFLEEDKFLVFVP"
CDS 1921..2844
/codon_start=1
/gene="luxD"
/product="LuxD acyltransferase"
/label=LuxD
/note="from the Photorhabdus luminescens bioluminescence
operon"
/translation="MENESKYKTIDHVICVEGNKKIHVWETLPEENSPKRKNAIIIASG
FARRMDHFAGLAEYLSRNGFHVIRYDSLHHVGLSSGTIDEFTMSIGKQSLLAVVDWLTT
RKINNFGMLASSLSARIAYASLSEINASFLITAVGVVNLRYSLERALGFDYLSLPINEL
PNNLDFEGHKLGAEVFARDCLDFGWEDLASTINNMMYLDIPFIAFTANNDNWVKQDEVI
TLLSNIRSNRCKIYSLLGSSHDLSENLVVLRNFYQSVTKAAIAMDNDHLDIDVDITEPS
FEHLTIATVNERRMRIEIENQAISLS"
CDS 2893..3975
/codon_start=1
/gene="luxA"
/product="LuxA luciferase subunit"
/label=LuxA
/note="from the Photorhabdus luminescens bioluminescence
operon"
/translation="MKFGNFLLTYQPPQFSQTEVMKRLVKLGRISEECGFDTVWLLEHH
FTEFGLLGNPYVAAAYLLGATKKLNVGTAAIVLPTAHPVRQLEDVNLLDQMSKGRFRFG
ICRGLYNKDFRVFGTDMNNSRALAECWYGLIKNGMTEGYMEADNEHIKFHKVKVNPAAY
SRGGAPVYVVAESASTTEWAAQFGLPMILSWIINTNEKKAQLELYNEVAQEYGHDIHNI
DHCLSYITSVDHDSIKAKEICRKFLGHWYDSYVNATTIFDDSDQTRGYDFNKGQWRDFV
LKGHKDTNRRIDYSYEINPVGTPQECIDIIQKDIDATGISNICCGFEANGTVDEIIASM
KLFQSDVMPFLKEKQRSLLY"
CDS 3990..4973
/codon_start=1
/gene="luxB"
/product="LuxB luciferase subunit"
/label=LuxB
/note="from the Photorhabdus luminescens bioluminescence
operon"
/translation="MKFGLFFLNFINSTTVQEQSIVRMQEITEYVDKLNFEQILVYENH
FSDNGVVGAPLTVSGFLLGLTEKIKIGSLNHIITTHHPVRIAEEACLLDQLSEGRFILG
FSDCEKKDEMHFFNRPVEYQQQLFEECYEIINDALTTGYCNPDNDFYSFPKISVNPHAY
TPGGPRKYVTATSHHIVEWAAKKGIPLIFKWDDSNDVRYEYAERYKAVADKYDVDLSEI
DHQLMILVNYNEDSNKAKQETRAFISDYVLEMHPNENFENKLEEIIAENAVGNYTECIT
AAKLAIEKCGAKSVLLSFEPMNDLMSQKNVINIVDDNIKKYHMEYT"
CDS 5152..6264
/codon_start=1
/gene="luxE"
/product="LuxE"
/label=LuxE
/note="from the Photorhabdus luminescens bioluminescence
operon"
/translation="MTSYVDKQEITASSEIDDLIFSSDPLVWSYDEQEKIRKKLVLDAF
RNHYKHCREYRHYCQAHKVDDNITEIDDIPVFPTSVFKFTRLLTSQENEIESWFTSSGT
NGLKSQVARDRLSIERLLGSVSYGMKYVGSWFDHQIELVNLGPDRFNAHNIWFKYVMSL
VELLYPTTFTVTEERIDFVKTLNSLERIKNQGKDLCLIGSPYFIYLLCHYMKDKKISFS
GDKSLYIITGGGWKSYEKESLKRDDFNHLLFDTFNLSDISQIRDIFNQVELNTCFFEDE
MQRKHVPPWVYARALDPETLKPVPDGTPGLMSYMDASATSYPAFIVTDDVGIISREYGK
YPGVLVEILRRVNTRTQKGCALSLTEAFDS"
primer_bind complement(6309..6325)
/label=SK primer
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(6309..6325)
/label=pBluescriptSK
/note="For pBluescript vector"
primer_bind complement(6357..6376)
/label=T7
/note="T7 promoter, forward primer"
promoter complement(6358..6376)
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
primer_bind complement(6386..6403)
/label=M13 Forward
/note="In lacZ gene. Also called M13-F20 or M13 (-21)
Forward"
primer_bind complement(6386..6402)
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(6395..6417)
/label=M13/pUC Forward
/note="In lacZ gene"
CDS complement(7111..7773)
/codon_start=1
/product="replication protein for the broad-host-range
plasmid pBBR1 from Bordetella bronchiseptica"
/label=pBBR1 Rep
/translation="MATQSREIGIQAKNKPGHWVQTERKAHEAWAGLIARKPTAAMLLH
HLVAQMGHQNAVVVSQKTLSKLIGRSLRTVQYAVKDLVAERWISVVKLNGPGTVSAYVV
NDRVAWGQPRDQLRLSVFSAAVVVDHDDQDESLLGHGDLRRIPTLYPGEQQLPTGPGEE
PPSQPGIPGMEPDLPALTETEEWERRGQQRLPMPDEPCFLDDGEPLEPPTRVTLPRR"
rep_origin 7774..8545
/label=pBBR1 oriV
/note="replication origin of the broad-host-range plasmid
pBBR1 from Bordetella bronchiseptica; requires the pBBR1
Rep protein for replication"
CDS complement(9870..10730)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 10493..10512
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(10731..1)
/gene="bla"
/label=AmpR promoter
ORIGIN
1 gggcaaatat tatacgcaag gcgacaaggt gctgatgccg ctggcgattc aggttcatca
61 tgccgtttgt gatggcttcc atgtcggcag aatgcttaat gaattacaac agtttttatg
121 catgcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg
181 gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta
241 gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta tgttgtgtgg
301 aattgtgagc ggataacaat ttcacacagg aaacagctat gaccatgatt acgccaagcg
361 cgcaattaac cctcactaaa gggaacaaaa gctgggtacc gggccccccc tcgaggtcga
421 cggtatcgat aagcttgata tcgaattcag gcttggagga tacgtatgac taaaaaaatt
481 tcattcatta ttaacggcca ggttgaaatc tttcccgaag gtgatgattt agtgcaatcc
541 attaattttg gtgataatag tgtttacctg ccaatattga atgactctca tgtaaaaaac
601 attattgatt gtaatggaaa taacgaatta cggttgcata acattgtcaa ttttctctat
661 acggtagggc aaagatggaa aaatgaagaa tactcaagac gcaggacata cattcgtgac
721 ttaaaaaaat atatgggata ttcagaagaa atggctaagc tagaggccaa ttggatatct
781 atgattttat gttctaaagg cggcctttat gatgttgtag aaaatgaact tggttctcgc
841 catatcatgg atgaatggct acctcaggat gaaagttatg ttcgggcttt tccgaaaggt
901 aaatctgtac atctgttggc aggtaatgtt ccattatctg ggatcatgtc tatattacgc
961 gcaattttaa ctaagaatca gtgtattata aaaacatcgt caaccgatcc ttttaccgct
1021 aatgcattag cgttaagttt tattgatgta gaccctaatc atccgataac gcgctcttta
1081 tctgttatat attggcccca ccaaggtgat acatcactcg caaaagaaat tatgcaacat
1141 gcggatgtta ttgtcgcttg gggagggcca gatgcgatta attgggcggt agagcatgcg
1201 ccatcttatg ctgatgtgat taaatttggt tctaaaaaga gtctttgcat tatcgataat
1261 cctgttgatt tgacgtccgc agcgacaggt gcggctcatg atgtttgttt ttacgatcag
1321 cgagcttgtt tttctgccca aaacatatat tacatgggaa atcattatga ggaatttaag
1381 ttagcgttga tagaaaaact taatctatat gcgcatatat taccgaatgc caaaaaagat
1441 tttgatgaaa aggcggccta ttctttagtt caaaaagaaa gcttgtttgc tggattaaaa
1501 gtagaggtgg atattcatca acgttggatg attattgagt caaatgcagg tgtggaattt
1561 aatcaaccac ttggcagatg tgtgtacctt catcacgtcg ataatattga gcaaatattg
1621 ccttatgttc aaaaaaataa gacgcaaacc atatctattt ttccttggga gtcatcattt
1681 aaatatcgag atgcgttagc attaaaaggt gcggaaagga ttgtagaagc aggaatgaat
1741 aacatatttc gagttggtgg atctcatgac ggaatgagac cgttgcaacg attagtgaca
1801 tatatttctc atgaaaggcc atctaactat acggctaagg atgttgcggt tgaaatagaa
1861 cagactcgat tcctggaaga agataagttc cttgtatttg tcccataata ggtaaaaagt
1921 atggaaaatg aatcaaaata taaaaccatc gaccacgtta tttgtgttga aggaaataaa
1981 aaaattcatg tttgggaaac gctgccagaa gaaaacagcc caaagagaaa gaatgccatt
2041 attattgcgt ctggttttgc ccgcaggatg gatcattttg ctggtctggc ggaatattta
2101 tcgcggaatg gatttcatgt gatccgctat gattcgcttc accacgttgg attgagttca
2161 gggacaattg atgaatttac aatgtctata ggaaagcaga gcttgttagc agtggttgat
2221 tggttaacta cacgaaaaat aaataacttc ggtatgttgg cttcaagctt atctgcgcgg
2281 atagcttatg caagcctatc tgaaatcaat gcttcgtttt taatcaccgc agtcggtgtt
2341 gttaacttaa gatattctct tgaaagagct ttagggtttg attatctcag tctacccatt
2401 aatgaattgc cgaataatct agattttgaa ggccataaat tgggtgctga agtctttgcg
2461 agagattgtc ttgattttgg ttgggaagat ttagcttcta caattaataa catgatgtat
2521 cttgatatac cgtttattgc ttttactgca aataacgata attgggtcaa gcaagatgaa
2581 gttatcacat tgttatcaaa tattcgtagt aatcgatgca agatatattc tttgttagga
2641 agttcgcatg acttgagtga aaatttagtg gtcctgcgca atttttatca atcggttacg
2701 aaagccgcta tcgcgatgga taatgatcat ctggatattg atgttgatat tactgaaccg
2761 tcatttgaac atttaactat tgcgacagtc aatgaacgcc gaatgagaat tgagattgaa
2821 aatcaagcaa tttctctgtc ttaaaatcta ttgagatatt ctatcactca aatagcaata
2881 taaggactct ctatgaaatt tggaaacttt ttgcttacat accaacctcc ccaattttct
2941 caaacagagg taatgaaacg tttggttaaa ttaggtcgca tctctgagga gtgtggtttt
3001 gataccgtat ggttactgga gcatcatttc acggagtttg gtttgcttgg taacccttat
3061 gtcgctgctg catatttact tggcgcgact aaaaaattga atgtaggaac tgccgctatt
3121 gttcttccca cagcccatcc agtacgccaa cttgaagatg tgaatttatt ggatcaaatg
3181 tcaaaaggac gatttcggtt tggtatttgc cgagggcttt acaacaagga ctttcgcgta
3241 ttcggcacag atatgaataa cagtcgcgcc ttagcggaat gctggtacgg gctgataaag
3301 aatggcatga cagagggata tatggaagct gataatgaac atatcaagtt ccataaggta
3361 aaagtaaacc ccgcggcgta tagcagaggt ggcgcaccgg tttatgtggt ggctgaatca
3421 gcttcgacga ctgagtgggc tgctcaattt ggcctaccga tgatattaag ttggattata
3481 aatactaacg aaaagaaagc acaacttgag ctttataatg aagtggctca agaatatggg
3541 cacgatattc ataatatcga ccattgctta tcatatataa catctgtaga tcatgactca
3601 attaaagcga aagagatttg ccggaaattt ctggggcatt ggtatgattc ttatgtgaat
3661 gctacgacta tttttgatga ttcagaccaa acaagaggtt atgatttcaa taaagggcag
3721 tggcgtgact ttgtattaaa aggacataaa gatactaatc gccgtattga ttacagttac
3781 gaaatcaatc ccgtgggaac gccgcaggaa tgtattgaca taattcaaaa agacattgat
3841 gctacaggaa tatcaaatat ttgttgtgga tttgaagcta atggaacagt agacgaaatt
3901 attgcttcca tgaagctctt ccagtctgat gtcatgccat ttcttaaaga aaaacaacgt
3961 tcgctattat attagctaag gagaaagaaa tgaaatttgg attgttcttc cttaacttca
4021 tcaattcaac aactgttcaa gaacaaagta tagttcgcat gcaggaaata acggagtatg
4081 ttgataagtt gaattttgaa cagattttag tgtatgaaaa tcatttttca gataatggtg
4141 ttgtcggcgc tcctctgact gtttctggtt ttctgctcgg tttaacagag aaaattaaaa
4201 ttggttcatt aaatcacatc attacaactc atcatcctgt ccgcatagcg gaggaagctt
4261 gcttattgga tcagttaagt gaagggagat ttattttagg gtttagtgat tgcgaaaaaa
4321 aagatgaaat gcattttttt aatcgcccgg ttgaatatca acagcaacta tttgaagagt
4381 gttatgaaat cattaacgat gctttaacaa caggctattg taatccagat aacgattttt
4441 atagcttccc taaaatatct gtaaatcccc atgcttatac gccaggcgga cctcggaaat
4501 atgtaacagc aaccagtcat catattgttg agtgggcggc caaaaaaggt attcctctca
4561 tctttaagtg ggatgattct aatgatgtta gatatgaata tgctgaaaga tataaagccg
4621 ttgcggataa atatgacgtt gacctatcag agatagacca tcagttaatg atattagtta
4681 actataacga agatagtaat aaagctaaac aagagacgcg tgcatttatt agtgattatg
4741 ttcttgaaat gcaccctaat gaaaatttcg aaaataaact tgaagaaata attgcagaaa
4801 acgctgtcgg aaattatacg gagtgtataa ctgcggctaa gttggcaatt gaaaagtgtg
4861 gtgcgaaaag tgtattgctg tcctttgaac caatgaatga tttgatgagc caaaaaaatg
4921 taatcaatat tgttgatgat aatattaaga agtaccacat ggaatatacc taatagattt
4981 cgagttgcag cgaggcggca agtgaacgaa tccccaggag catagataac tatgtgactg
5041 gggtgagtga aagcagccaa caaagcagca gcttgaaaga tgaagggtat aaaagagtat
5101 gacagcagtg ctgccatact ttctaatatt atcttgagga gtaaaacagg tatgacttca
5161 tatgttgata aacaagaaat tacagcaagc tcagaaattg atgatttgat tttttcgagc
5221 gatccattag tgtggtctta cgacgagcag gaaaaaatca gaaagaaact tgtgcttgat
5281 gcatttcgta atcattataa acattgtcga gaatatcgtc actactgtca ggcacacaaa
5341 gtagatgaca atattacgga aattgatgac atacctgtat tcccaacatc ggtttttaag
5401 tttactcgct tattaacttc tcaggaaaac gagattgaaa gttggtttac cagtagcggc
5461 acgaatggtt taaaaagtca ggtggcgcgt gacagattaa gtattgagag actcttaggc
5521 tctgtgagtt atggcatgaa atatgttggt agttggtttg atcatcaaat agaattagtc
5581 aatttgggac cagatagatt taatgctcat aatatttggt ttaaatatgt tatgagtttg
5641 gtggaattgt tatatcctac gacatttacc gtaacagaag aacgaataga ttttgttaaa
5701 acattgaata gtcttgaacg aataaaaaat caagggaaag atctttgtct tattggttcg
5761 ccatacttta tttatttact ctgccattat atgaaagata aaaaaatctc attttctgga
5821 gataaaagcc tttatatcat aaccggaggc ggctggaaaa gttacgaaaa agaatctctg
5881 aaacgtgatg atttcaatca tcttttattt gatactttca atctcagtga tattagtcag
5941 atccgagata tatttaatca agttgaactc aacacttgtt tctttgagga tgaaatgcag
6001 cgtaaacatg ttccgccgtg ggtatatgcg cgagcgcttg atcctgaaac gttgaaacct
6061 gtacctgatg gaacgccggg gttgatgagt tatatggatg cgtcagcaac cagttatcca
6121 gcatttattg ttaccgatga tgtcgggata attagcagag aatatggtaa gtatcccggc
6181 gtgctcgttg aaattttacg tcgcgtcaat acgaggacgc agaaagggtg tgctttaagc
6241 ttaaccgaag cgtttgatag ttgatgacct gcaggtcgac ggatccgggg aattcctgca
6301 gcccggggga tccactagtt ctagagcggc cgccaccgcg gtggagctcc aattcgccct
6361 atagtgagtc gtattacgcg cgctcactgg ccgtcgtttt acaacgtcgt gactgggaaa
6421 accctggcgt tacccaactt aatcgccttg cagcacatcc ccctttcgcc agctggcgta
6481 atagcgaaga ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg aatggcgaat
6541 ggaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
6601 attttttaac caataggccg tactgcgatg agtggcaggg cggggcgtaa tttttttaag
6661 gcagttattg gtgcccttaa acgcctggtt gctacgcctg aataagtgat aataagcgga
6721 tgaatggcag aaattcgaaa gcaaattcga cccggtcgtc ggttcagggc agggtcgtta
6781 aatagccgct tatgtctatt gctggtttac cggtttattg actaccggaa gcagtgtgac
6841 cgtgtgcttc tcaaatgcct gaggccagtt tgctcaggct ctccccgtgg aggtaataat
6901 tgacgatatg atcatttatt ctgcctccca gagcctgata aaaacggtga atccgttagc
6961 gaggtgccgc cggcttccat tcaggtcgag gtggcccggc tccatgcacc gcgacgcaac
7021 gcggggaggc agacaaggta tagggcggcg aggcggctac agccgatagt ctggaacagc
7081 gcacttacgg gttgctgcgc aacccaagtg ctaccggcgc ggcagcgtga cccgtgtcgg
7141 cggctccaac ggctcgccat cgtccagaaa acacggctca tcgggcatcg gcaggcgctg
7201 ctgcccgcgc cgttcccatt cctccgtttc ggtcaaggct ggcaggtctg gttccatgcc
7261 cggaatgccg ggctggctgg gcggctcctc gccggggccg gtcggtagtt gctgctcgcc
7321 cggatacagg gtcgggatgc ggcgcaggtc gccatgcccc aacagcgatt cgtcctggtc
7381 gtcgtgatca accaccacgg cggcactgaa caccgacagg cgcaactggt cgcggggctg
7441 gccccacgcc acgcggtcat tgaccacgta ggccgacacg gtgccggggc cgttgagctt
7501 cacgacggag atccagcgct cggccaccaa gtccttgact gcgtattgga ccgtccgcaa
7561 agaacgtccg atgagcttgg aaagtgtctt ctggctgacc accacggcgt tctggtggcc
7621 catctgcgcc acgaggtgat gcagcagcat tgccgccgtg ggtttcctcg caataagccc
7681 ggcccacgcc tcatgcgctt tgcgttccgt ttgcacccag tgaccgggct tgttcttggc
7741 ttgaatgccg atttctctgg actgcgtggc catgcttatc tccatgcggt aggggtgccg
7801 cacggttgcg gcaccatgcg caatcagctg caacttttcg gcagcgcgac aacaattatg
7861 cgttgcgtaa aagtggcagt caattacaga ttttctttaa cctacgcaat gagctattgc
7921 ggggggtgcc gcaatgagct gttgcgtacc cccctttttt aagttgttga tttttaagtc
7981 tttcgcattt cgccctatat ctagttcttt ggtgcccaaa gaagggcacc cctgcggggt
8041 tcccccacgc cttcggcgcg gctccccctc cggcaaaaag tggcccctcc ggggcttgtt
8101 gatcgactgc gcggccttcg gccttgccca aggtggcgct gcccccttgg aacccccgca
8161 ctcgccgccg tgaggctcgg ggggcaggcg ggcgggcttc gcccttcgac tgcccccact
8221 cgcataggct tgggtcgttc caggcgcgtc aaggccaagc cgctgcgcgg tcgctgcgcg
8281 agccttgacc cgccttccac ttggtgtcca accggcaagc gaagcgcgca ggccgcaggc
8341 cggaggcttt tccccagaga aaattaaaaa aattgatggg gcaaggccgc aggccgcgca
8401 gttggagccg gtgggtatgt ggtcgaaggc tgggtagccg gtgggcaatc cctgtggtca
8461 agctcgtggg caggcgcagc ctgtccatca gcttgtccag cagggttgtc cacgggccga
8521 gcgaagcgag ccagccggtg gccgctcgcg gccatcgtcc acatatccac gggctggcaa
8581 gggagcgcag cgaccgcgca gggcgaagcc cggagagcaa gcccgtaggg cgccgcagcc
8641 gccgtaggcg gtcacgactt tgcgaagcaa agtctagtga gtatactcaa gcattgagtg
8701 gcccgccgga ggcaccgcct tgcgctgccc ccgtcgagcc ggttggacac caaaagggag
8761 gggcaggcat ggcggcatac gcgatcatgc gatgcaagaa gctggcgaaa atgggcaacg
8821 tggcggccag tctcaagcac gcctaccgcg agcgcgagac gcccaacgct gacgccagca
8881 ggacgccaga gaacgagcac tgggcggcca gcagcaccga tgaagcgatg ggccgactgc
8941 gcgagttgct gccagagaag cggcgcaagg acgctgtgtt ggcggtcgag tacgtcatga
9001 cggccagccc ggaatggtgg aagtcggcca gccaagaaca gcaggcggcg ttcttcgaga
9061 aggcgcacaa gtggctggcg gacaagtacg gggcggatcg catcgtgacg gccagcatcc
9121 accgtgacga aaccagcccg cacatgaccg cgttcgtggt gccgctgacg caggacggca
9181 ggctgtcggc caaggagttc atcggcaaca aagcgcagat gacccgcgac cagaccacgt
9241 ttgcggccgc tgtggccgat ctagggctgc aacggggcat cgagggcagc aaggcacgtc
9301 acacgcgcat tcaggcgttc tacgaggccc tggagcggcc accagtgggc cacgtcacca
9361 tcagcccgca agcggtcgag ccacgcgcct atgcaccgca gggattggcc gaaaagctgg
9421 gaatctcaaa gcgcgttgag acgccggaag ccgtggccga ccggctgaca aaagcggttc
9481 ggcaggggta tgagcctgcc ctacaggccg ccgcaggagc gcgtgagatg cgcaagaagg
9541 ccgatcaagc ccaagagacg gcccgagacc ttcgggagcg cctgaagccc gttctggacg
9601 ccctggggcc gttgaatcgg gatatgcagg ccaaggccgc cgcgatcatc aaggccgtgg
9661 gcgaaaagct gctgacggaa cagcgggaag tccagcgcca gaaacaggcc cagcgccagc
9721 aggaacgcgg gcgcgcacat ttccccgaaa agtgccacct ggattttggt catgagatta
9781 tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa
9841 agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc
9901 tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact
9961 acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc
10021 tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt
10081 ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta
10141 agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg
10201 tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt
10261 acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc
10321 agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt
10381 actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc
10441 tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc
10501 gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa
10561 ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac
10621 tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa
10681 aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt
10741 tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa
10801 t
//
