| 质粒名称: | pMaster3 |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 58526 |
| 存储实验室: | Mario Capecchi |
|
载体骨架基本信息 |
|
| 载体名称: | pCEP4 |
| 载体出品公司: | -- |
| 载体抗性: | 氨苄青霉素 |
| 空载体大小: | -- |
| 完整质粒大小: | -- |
| 载体修饰: | -- |
| 载体类型: | -- |
| 筛选标记: | 新霉素（G418） |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | -- |
| 物种来源: | -- |
| 插入大小: | 816 bp |
| 基因突变: | -- |
| GenBank编号 | -- |
| 启动子 | -- |
| 标签/融合蛋白: | -- |
| 克隆方法: | -- |
| 5' 克隆位点: | -- |
| 3' 克隆位点: | -- |
| 5' 测序引物 | -- |
| 3' 测序引物 | -- |
|
文献引用方法 |
|
| 材料和方法部分: | pMaster3 was a gift from Mario Capecchi (Addgene plasmid # 58526 ; http://n2t.net/addgene:58526 ; RRID:Addgene_58526) |
| 参考文献部分: | Efficient germ-line transmission obtained with transgene-free induced pluripotent stem cells. Wu S, Wu Y, Zhang X, Capecchi MR. Proc Natl Acad Sci U S A. 2014 Jul 22;111(29):10678-83. doi: 10.1073/pnas.1409933111. Epub 2014 Jul 7. 10.1073/pnas.1409933111 PubMed 25002522 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pMaster3 24152 bp ds-DNA circular SYN 12-MAY-2021
DEFINITION Reprogram somatic cells to iPS cells.
ACCESSION .
VERSION .
KEYWORDS pMaster3
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 24152)
AUTHORS Wu S, Wu Y, Zhang X, Capecchi MR
TITLE Efficient germ-line transmission obtained with transgene-free
induced pluripotent stem cells.
JOURNAL Proc Natl Acad Sci U S A. 2014 Jul 22;111(29):10678-83. doi:
10.1073/pnas.1409933111. Epub 2014 Jul 7.
PUBMED 25002522
REFERENCE 2 (bases 1 to 24152)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..24152
/organism="synthetic DNA construct"
/mol_type="other DNA"
promoter 359..636
/label=chicken beta-actin promoter
intron 637..1653
/label=chimeric intron
/note="chimera between introns from chicken beta-actin and
rabbit beta-globin"
CDS 2646..2711
/codon_start=1
/product="2A peptide from foot-and-mouth disease virus
polyprotein"
/label=F2A
/note="Eukaryotic ribosomes fail to insert a peptide bond
between the Gly and Pro residues, yielding separate
polypeptides."
/translation="VKQTLNFDLLKLAGDVESNPGP"
rep_origin complement(3523..4111)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(3603..3622)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(4282..5142)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 4905..4924
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(5143..5247)
/gene="bla"
/label=AmpR promoter
primer_bind 5315..5333
/label=pBRforEco
/note="pBR322 vectors, upsteam of EcoRI site, forward
primer"
rep_origin 7371..9160
/label=oriP
/note="Epstein-Barr virus oriP replication origin (Yates et
al., 2000)"
promoter 9636..10814
/label=EF-1-alpha promoter
/note="strong constitutive promoter for human elongation
factor EF-1-alpha"
intron 9867..10805
/label=EF-1-alpha intron A
/note="intron upstream of the start codon of human
EF-1-alpha"
regulatory 10822..10831
/regulatory_class="other"
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
CDS 11917..11982
/codon_start=1
/product="2A peptide from foot-and-mouth disease virus
polyprotein"
/label=F2A
/note="Eukaryotic ribosomes fail to insert a peptide bond
between the Gly and Pro residues, yielding separate
polypeptides."
/translation="VKQTLNFDLLKLAGDVESNPGP"
CDS 13402..13455
/codon_start=1
/product="2A peptide from Thosea asigna virus capsid
protein"
/label=T2A
/note="Eukaryotic ribosomes fail to insert a peptide bond
between the Gly and Pro residues, yielding separate
polypeptides."
/translation="EGRGSLLTCGDVEENPGP"
CDS 14416..14475
/codon_start=1
/product="2A peptide from equine rhinitis A virus
polyprotein"
/label=E2A
/note="Eukaryotic ribosomes fail to insert a peptide bond
between the Gly and Pro residues, yielding separate
polypeptides."
/translation="QCTNYALLKLAGDVESNPGP"
CDS 14476..15795
/codon_start=1
/product="human c-Myc proto-oncogene"
/label=c-Myc
/translation="MPLNVSFTNRNYDLDYDSVQPYFYCDEEENFYQQQQQSELQPPAP
SEDIWKKFELLPTPPLSPSRRSGLCSPSYVAVTPFSLRGDNDGGGGSFSTADQLEMVTE
LLGGDMVNQSFICDPDDETFIKNIIIQDCMWSGFSAAAKLVSEKLASYQAARKDSGSPN
PARGHSVCSTSSLYLQDLSAAASECIDPSVVFPYPLNDSSSPKSCASQDSSAFSPSSDS
LLSSTESSPQGSPEPLVLHEETPPTTSSDSEEEQEDEEEIDVVSVEKRQAPGKRSESGS
PSAGGHSKPPHSPLVLKRCHVSTHQHNYAAPPSTRKDYPAAKRVKLDSVRVLRQISNNR
KCTSPRSSDTEENVKRRTHNVLERQRRNELKRSFFALRDQIPELENNEKAPKVVILKKA
TAYILSVQAEEQKLISEEDLLRKRREQLKHKLEQLRNSCA"
polyA_signal 15919..16040
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(15956..15975)
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind 16010..16029
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
rep_origin complement(16047..16502)
/direction=LEFT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(16184..16205)
/label=F1ori-F
/note="F1 origin, forward primer"
primer_bind 16396..16415
/label=F1ori-R
/note="F1 origin, reverse primer"
enhancer 16628..16931
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 16933..17210
/label=chicken beta-actin promoter
intron 17211..18227
/label=chimeric intron
/note="chimera between introns from chicken beta-actin and
rabbit beta-globin"
CDS 18300..19430
/codon_start=1
/product="herpes simplex virus thymidine kinase"
/label=HSV TK
/note="confers sensitivity to nucleoside analogs such as
acyclovir or ganciclovir"
/translation="MASYPGHQHASAFDQAARSRGHSNRRTALRPRRQQEATEVRPEQK
MPTLLRVYIDGPHGMGKTTTTQLLVALGSRDDIVYVPEPMTYWRVLGASETIANIYTTQ
HRLDQGEISAGDAAVVMTSAQITMGMPYAVTDAVLAPHIGGEAGSSHAPPPALTLIFDR
HPIAALLCYPAARYLMGSMTPQAVLAFVALIPPTLPGTNIVLGALPEDRHIDRLAKRQR
PGERLDLAMLAAIRRVYGLLANTVRYLQCGGSWREDWGQLSGTAVPPQGAEPQSNAGPR
PHIGDTLFTLFRAPELLAPNGDLYNVFAWALDVLAKRLRSMHVFILDYDQSPAGCRDAL
LQLTSGMVQTHVTTPGSIPTICDLARTFAREMGEAN"
misc_feature 19446..20032
/label=IRES2
/note="internal ribosome entry site (IRES) of the
encephalomyocarditis virus (EMCV)"
primer_bind complement(19613..19630)
/label=IRES reverse
/note="IRES internal ribosome entry site, reverse primer.
Also called pCDH-rev"
primer_bind 19840..19859
/label=IRES-F
/note="IRES internal ribosome entry site, forward primer"
CDS 20033..20836
/codon_start=1
/gene="aph(3')-II (or nptII)"
/product="aminoglycoside phosphotransferase from Tn5"
/label=NeoR/KanR
/note="confers resistance to neomycin, kanamycin, and G418
(Geneticin(R))"
/translation="MGSAIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQ
GRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQD
LLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
EHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQ
DIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
primer_bind complement(20096..20115)
/label=Neo-R
/note="Neomycin resistance gene, reverse primer"
primer_bind 20706..20725
/label=Neo-F
/note="Neomycin resistance gene, forward primer"
polyA_signal 21068..21115
/label=HSV TK poly(A) signal
/note="herpes simplex virus thymidine kinase
polyadenylation signal (Cole and Stacy, 1985)"
promoter 21136..22314
/label=EF-1-alpha promoter
/note="strong constitutive promoter for human elongation
factor EF-1-alpha"
intron 21367..22305
/label=EF-1-alpha intron A
/note="intron upstream of the start codon of human
EF-1-alpha"
polyA_signal 24071..24118
/label=HSV TK poly(A) signal
/note="herpes simplex virus thymidine kinase
polyadenylation signal (Cole and Stacy, 1985)"
enhancer 24130..357
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
ORIGIN
1 taatagtaat caattacggg gtcattagtt catagcccat atatggagtt ccgcgttaca
61 taacttacgg taaatggccc gcctggctga ccgcccaacg acccccgccc attgacgtca
121 ataatgacgt atgttcccat agtaacgcca atagggactt tccattgacg tcaatgggtg
181 gagtatttac ggtaaactgc ccacttggca gtacatcaag tgtatcatat gccaagtacg
241 ccccctattg acgtcaatga cggtaaatgg cccgcctggc attatgccca gtacatgacc
301 ttatgggact ttcctacttg gcagtacatc tacgtattag tcatcgctat taccatggtc
361 gaggtgagcc ccacgttctg cttcactctc cccatctccc ccccctcccc acccccaatt
421 ttgtatttat ttatttttta attattttgt gcagcgatgg gggcgggggg gggggggggg
481 cgcgcgccag gcggggcggg gcggggcgag gggcggggcg gggcgaggcg gagaggtgcg
541 gcggcagcca atcagagcgg cgcgctccga aagtttcctt ttatggcgag gcggcggcgg
601 cggcggccct ataaaaagcg aagcgcgcgg cgggcgggag tcgctgcgcg ctgccttcgc
661 cccgtgcccc gctccgccgc cgcctcgcgc cgcccgcccc ggctctgact gaccgcgtta
721 ctcccacagg tgagcgggcg ggacggccct tctcctccgg gctgtaatta gcgcttggtt
781 taatgacggc ttgtttcttt tctgtggctg cgtgaaagcc ttgaggggct ccgggagggc
841 cctttgtgcg gggggagcgg ctcggggggt gcgtgcgtgt gtgtgtgcgt ggggagcgcc
901 gcgtgcggct ccgcgctgcc cggcggctgt gagcgctgcg ggcgcggcgc ggggctttgt
961 gcgctccgca gtgtgcgcga ggggagcgcg gccgggggcg gtgccccgcg gtgcgggggg
1021 ggctgcgagg ggaacaaagg ctgcgtgcgg ggtgtgtgcg tgggggggtg agcagggggt
1081 gtgggcgcgt cggtcgggct gcaacccccc ctgcaccccc ctccccgagt tgctgagcac
1141 ggcccggctt cgggtgcggg gctccgtacg gggcgtggcg cggggctcgc cgtgccgggc
1201 ggggggtggc ggcaggtggg ggtgccgggc ggggcggggc cgcctcgggc cggggagggc
1261 tcgggggagg ggcgcggcgg cccccggagc gccggcggct gtcgaggcgc ggcgagccgc
1321 agccattgcc ttttatggta atcgtgcgag agggcgcagg gacttccttt gtcccaaatc
1381 tgtgcggagc cgaaatctgg gaggcgccgc cgcaccccct ctagcgggcg cggggcgaag
1441 cggtgcggcg ccggcaggaa ggaaatgggc ggggagggcc ttcgtgcgtc gccgcgccgc
1501 cgtccccttc tccctctcca gcctcggggc tgtccgcggg gggacggctg ccttcggggg
1561 ggacggggca gggcggggtt cggcttctgg cgtgtgaccg gcggctctag agcctctgct
1621 aaccatgttc atgccttctt ctttttccta cagctcctgg gcaacgtgct ggttattgtg
1681 ctgtctcatc attttggcaa agaattcgcg attaagccac catgagtgtg gatccagctt
1741 gtccccaaag cttgccttgc tttgaagcat ccgactgtaa agaatcttca cctatgcctg
1801 tgatttgtgg gcctgaagaa aactatccat ccttgcaaat gtcttctgct gagatgcctc
1861 acacggagac tgtctctcct cttccttcct ccatggatct gcttattcag gacagccctg
1921 attcttccac cagtcccaaa ggcaaacaac ccacttctgc agagaagagt gtcgcaaaaa
1981 aggaagacaa ggtcccggtc aagaaacaga agaccagaac tgtgttctct tccacccagc
2041 tgtgtgtact caatgataga tttcagagac agaaatacct cagcctccag cagatgcaag
2101 aactctccaa catcctgaac ctcagctaca aacaggtgaa gacctggttc cagaaccaga
2161 gaatgaaatc taagaggtgg cagaaaaaca actggccgaa gaatagcaat ggtgtgacgc
2221 agaaggcctc agcacctacc taccccagcc tttactcttc ctaccaccag ggatgcctgg
2281 tgaacccgac tgggaacctt ccaatgtgga gcaaccagac ctggaacaat tcaacctgga
2341 gcaaccagac ccagaacatc cagtcctgga gcaaccactc ctggaacact cagacctggt
2401 gcacccaatc ctggaacaat caggcctgga acagtccctt ctataactgt ggagaggaat
2461 ctctgcagtc ctgcatgcag ttccagccaa attctcctgc cagtgacttg gaggctgcct
2521 tggaagctgc tggggaaggc cttaatgtaa tacagcagac cactaggtat tttagtactc
2581 cacaaaccat ggatttattc ctaaactact ccatgaacat gcaacctgaa gacgtgggaa
2641 gtggcgtgaa acagactttg aattttgacc ttctcaagtt ggcgggagac gtggagtcca
2701 acccagggcc catgggctcc gtgtccaacc agcagtttgc aggtggctgc gccaaggcgg
2761 cagaagaggc gcccgaggag gcgccggagg acgcggcccg ggcggcggac gagcctcagc
2821 tgctgcacgg tgcgggcatc tgtaagtggt tcaacgtgcg catggggttc ggcttcctgt
2881 ccatgaccgc ccgcgccggg gtcgcgctcg accccccagt ggatgtcttt gtgcaccaga
2941 gtaagctgca catggaaggg ttccggagct tgaaggaggg tgaggcagtg gagttcacct
3001 ttaagaagtc agccaagggt ctggaatcca tccgtgtcac cggacctggt ggagtattct
3061 gtattgggag tgagaggcgg ccaaaaggaa agagcatgca gaagcgcaga tcaaaaggag
3121 acaggtgcta caactgtgga ggtctagatc atcatgccaa ggaatgcaag ctgccacccc
3181 agcccaagaa gtgccacttc tgccagagca tcagccatat ggtagcctca tgtccgctga
3241 aggcccagca gggccctagt gcacagggaa agccaaccta ctttcgagag gaagaagaag
3301 aaatccacag ccctaccctg ctcccggagg cacagaattg ataagataaa cgtcgacgcg
3361 aggctggatg gccttcccca ttatgattct tctcgcttcc ggcggcatcg ggatgcccgc
3421 gttgcaggcc atgctgtcca ggcaggtaga tgacgaccat cagggacagc ttcaaggcca
3481 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc
3541 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact
3601 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct
3661 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag
3721 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca
3781 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa
3841 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc
3901 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag
3961 aaggacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg
4021 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca
4081 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc
4141 tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag
4201 gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata
4261 tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat
4321 ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg
4381 ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc
4441 tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc
4501 aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc
4561 gccagttaat agtttgcgca acgttgttgc cattgctgca ggcatcgtgg tgtcacgctc
4621 gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc
4681 ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa
4741 gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat
4801 gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata
4861 gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaaca cgggataata ccgcgccaca
4921 tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag
4981 gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc
5041 agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc
5101 aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata
5161 ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta
5221 gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtcta
5281 agaaaccatt attatcatga cattaaccta taaaaatagg cgtatcacga ggccctttcg
5341 tcttcaagaa ttctcatgtt tgacagctta tcatcgataa gctgatcctc acaggccgca
5401 cccagctttt cttccgttgc cccagtagca tctctgtctg gtgaccttga agaggaagag
5461 gaggggtccc gagaatcccc atccctaccg tccagcaaaa agggggacga ggaatttgag
5521 gcctggcttg aggctcagga cgcaaatctt gaggatgttc agcgggagtt ttccgggctg
5581 cgagtaattg gtgatgagga cgaggatggt tcggaggatg gggaattttc agacctggat
5641 ctgtctgaca gcgaccatga aggggatgag ggtggggggg ctgttggagg gggcaggagt
5701 ctgcactccc tgtattcact gagcgtcgtc taataaagat gtctattgat ctcttttagt
5761 gtgaatcatg tctgacgagg ggccaggtac aggacctgga aatggcctag gagagaaggg
5821 agacacatct ggaccagaag gctccggcgg cagtggacct caaagaagag ggggtgataa
5881 ccatggacga ggacggggaa gaggacgagg acgaggaggc ggaagaccag gagccccggg
5941 cggctcagga tcagggccaa gacatagaga tggtgtccgg agaccccaaa aacgtccaag
6001 ttgcattggc tgcaaaggga cccacggtgg aacaggagca ggagcaggag cgggaggggc
6061 aggagcagga ggggcaggag caggaggagg ggcaggagca ggaggggcag gagcaggagg
6121 tggaggccgg ggtcgaggag gcagtggagg ccggggtcga ggaggtagtg gaggccgggg
6181 tcgaggaggt agtggaggcc gccggggtag aggacgtgaa agagccaggg ggggaagtcg
6241 tgaaagagcc agggggagag gtcgtggacg tggagaaaag aggcccagga gtcccagtag
6301 tcagtcatca tcatccgggt ctccaccgcg caggccccct ccaggtagaa ggccattttt
6361 ccaccctgta ggggaagccg attattttga ataccaccaa gaaggtggcc cagatggtga
6421 gcctgacgtg cccccgggag cgatagagca gggccccgca gatgacccag gagaaggccc
6481 aagcactgga ccccggggtc agggtgatgg aggcaggcgc aaaaaaggag ggtggtttgg
6541 aaagcatcgt ggtcaaggag gttccaaccc gaaatttgag aacattgcag aaggtttaag
6601 agctctcctg gctaggagtc acgtagaaag gactaccgac gaaggaactt gggtcgccgg
6661 tgtgttcgta tatggaggta gtaagacctc cctttacaac ctaaggcgag gaactgccct
6721 tgctattcca caatgtcgtc ttacaccatt gagtcgtctc ccctttggaa tggcccctgg
6781 acccggccca caacctggcc cgctaaggga gtccattgtc tgttatttca tggtcttttt
6841 acaaactcat atatttgctg aggttttgaa ggatgcgatt aaggaccttg ttatgacaaa
6901 gcccgctcct acctgcaata tcagggtgac tgtgtgcagc tttgacgatg gagtagattt
6961 gcctccctgg tttccaccta tggtggaagg ggctgccgcg gagggtgatg acggagatga
7021 cggagatgaa ggaggtgatg gagatgaggg tgaggaaggg caggagtgat gtaacttgtt
7081 aggagacgcc ctcaatcgta ttaaaagccg tgtattcccc cgcactaaag aataaatccc
7141 cagtagacat catgcgtgct gttggtgtat ttctggccat ctgtcttgtc accattttcg
7201 tcctcccaac atggggcaat tgggcatacc catgttgtca cgtcactcag ctccgcgctc
7261 aacaccttct cgcgttggaa aacattagcg acatttacct ggtgagcaat cagacatgcg
7321 acggctttag cctggcctcc ttaaattcac ctaagaatgg gagcaaccag caggaaaagg
7381 acaagcagcg aaaattcacg cccccttggg aggtggcggc atatgcaaag gatagcactc
7441 ccactctact actgggtatc atatgctgac tgtatatgca tgaggatagc atatgctacc
7501 cggatacaga ttaggatagc atatactacc cagatataga ttaggatagc atatgctacc
7561 cagatataga ttaggatagc ctatgctacc cagatataaa ttaggatagc atatactacc
7621 cagatataga ttaggatagc atatgctacc cagatataga ttaggatagc ctatgctacc
7681 cagatataga ttaggatagc atatgctacc cagatataga ttaggatagc atatgctatc
7741 cagatatttg ggtagtatat gctacccaga tataaattag gatagcatat actaccctaa
7801 tctctattag gatagcatat gctacccgga tacagattag gatagcatat actacccaga
7861 tatagattag gatagcatat gctacccaga tatagattag gatagcctat gctacccaga
7921 tataaattag gatagcatat actacccaga tatagattag gatagcatat gctacccaga
7981 tatagattag gatagcctat gctacccaga tatagattag gatagcatat gctatccaga
8041 tatttgggta gtatatgcta cccatggcaa cattagccca ccgtgctctc agcgacctcg
8101 tgaatatgag gaccaacaac cctgtgcttg gcgctcaggc gcaagtgtgt gtaatttgtc
8161 ctccagatcg cagcaatcgc gcccctatct tggcccgccc acctacttat gcaggtattc
8221 cccggggtgc cattagtggt tttgtgggca agtggtttga ccgcagtggt tagcggggtt
8281 acaatcagcc aagttattac acccttattt tacagtccaa aaccgcaggg cggcgtgtgg
8341 gggctgacgc gtgcccccac tccacaattt caaaaaaaag agtggccact tgtctttgtt
8401 tatgggcccc attggcgtgg agccccgttt aattttcggg ggtgttagag acaaccagtg
8461 gagtccgctg ctgtcggcgt ccactctctt tccccttgtt acaaatagag tgtaacaaca
8521 tggttcacct gtcttggtcc ctgcctggga cacatcttaa taaccccagt atcatattgc
8581 actaggatta tgtgttgccc atagccataa attcgtgtga gatggacatc cagtctttac
8641 ggcttgtccc caccccatgg atttctattg ttaaagatat tcagaatgtt tcattcctac
8701 actagtattt attgcccaag gggtttgtga gggttatatt ggtgtcatag cacaatgcca
8761 ccactgaacc ccccgtccaa attttattct gggggcgtca cctgaaacct tgttttcgag
8821 cacctcacat acaccttact gttcacaact cagcagttat tctattagct aaacgaagga
8881 gaatgaagaa gcaggcgaag attcaggaga gttcactgcc cgctccttga tcttcagcca
8941 ctgcccttgt gactaaaatg gttcactacc ctcgtggaat cctgacccca tgtaaataaa
9001 accgtgacag ctcatggggt gggagatatc gctgttcctt aggacccttt tactaaccct
9061 aattcgatag catatgcttc ccgttgggta acatatgcta ttgaattagg gttagtctgg
9121 atagtatata ctactacccg ggaagcatat gctacccgtt tagggttaac aagggggcct
9181 tataaacact attgctaatg ccctcttgag ggtccgctta tcggtagcta cacaggcccc
9241 tctgattgac gttggtgtag cctcccgtag tcttcctggg cccctgggag gtacatgtcc
9301 cccagcattg gtgtaagagc ttcagccaag agttacacat aaaggcaatg ttgtgttgca
9361 gtccacagac tgcaaagtct gctccaggat gaaagccact cagtgttggc aaatgtgcac
9421 atccatttat aaggatgtca actacagtca gagaacccct ttgtgtttgg tccccccccg
9481 tgtcacatgt ggaacagggc ccagttggca agttgtacca accaactgaa gggattacat
9541 gcactgcccc gcgaagaagg ggcagagatg tcgtagtcag gtttagttcg tccggggcgg
9601 ggatcgatcc tctagagtcg attaattaat cgtgaggctc cggtgcccgt cagtgggcag
9661 agcgcacatc gcccacagtc cccgagaagt tggggggagg ggtcggcaat tgaaccggtg
9721 cctagagaag gtggcgcggg gtaaactggg aaagtgatgt cgtgtactgg ctccgccttt
9781 ttcccgaggg tgggggagaa ccgtatataa gtgcagtagt cgccgtgaac gttctttttc
9841 gcaacgggtt tgccgccaga acacaggtaa gtgccgtgtg tggttcccgc gggcctggcc
9901 tctttacggg ttatggccct tgcgtgcctt gaattacttc cacctggctg cagtacgtga
9961 ttcttgatcc cgagcttcgg gttggaagtg ggtgggagag ttcgaggcct tgcgcttaag
10021 gagccccttc gcctcgtgct tgagttgagg cctggcctgg gcgctggggc cgccgcgtgc
10081 gaatctggtg gcaccttcgc gcctgtctcg ctgctttcga taagtctcta gccatttaaa
10141 atttttgatg acctgctgcg acgctttttt tctggcaaga tagtcttgta aatgcgggcc
10201 aagatctgca cactggtatt tcggtttttg gggccgcggg cggcgacggg gcccgtgcgt
10261 cccagcgcac atgttcggcg aggcggggcc tgcgagcgcg gccaccgaga atcggacggg
10321 ggtagtctca agctggccgg cctgctctgg tgcctggcct cgcgccgccg tgtatcgccc
10381 cgccctgggc ggcaaggctg gcccggtcgg caccagttgc gtgagcggaa agatggccgc
10441 ttcccggccc tgctgcaggg agctcaaaat ggaggacgcg gcgctcggga gagcgggcgg
10501 gtgagtcacc cacacaaagg aaaagggcct ttccgtcctc agccgtcgct tcatgtgact
10561 ccacggagta ccgggcgccg tccaggcacc tcgattagtt ctcgagcttt tggagtacgt
10621 cgtctttagg ttggggggag gggttttatg cgatggagtt tccccacact gagtgggtgg
10681 agactgaagt taggccagct tggcacttga tgtaattctc cttggaattt gccctttttg
10741 agtttggatc ttggttcatt ctcaagcctc agacagtggt tcaaagtttt tttcttccat
10801 ttcaggtgtc gtgaagctag cgccaccatg gcgggacacc tggcttcgga tttcgccttc
10861 tcgccccctc caggtggtgg aggtgatggg ccaggggggc cggagccggg ctgggttgat
10921 cctcggacct ggctaagctt ccaaggccct cctggagggc caggaatcgg gccgggggtt
10981 gggccaggct ctgaggtgtg ggggattccc ccatgccccc cgccgtatga gttctgtggg
11041 gggatggcgt actgtgggcc ccaggttgga gtggggctag tgccccaagg cggcttggag
11101 acctctcagc ctgagggcga agcaggagtc ggggtggaga gcaactccga tggggcctcc
11161 ccggagccct gcaccgtcac ccctggtgcc gtgaagctgg agaaggagaa gctggagcaa
11221 aacccggagg agtcccagga catcaaagct ctgcagaaag aactcgagca atttgccaag
11281 ctcctgaagc agaagaggat caccctggga tatacacagg ccgatgtggg gctcaccctg
11341 ggggttctat ttgggaaggt attcagccaa acgaccatct gccgctttga ggctctgcag
11401 cttagcttca agaacatgtg taagctgcgg cccttgctgc agaagtgggt ggaggaagct
11461 gacaacaatg aaaatcttca ggagatatgc aaagcagaaa ccctcgtgca ggcccgaaag
11521 agaaagcgaa ccagtatcga gaaccgagtg agaggcaacc tggagaattt gttcctgcag
11581 tgcccgaaac ccacactgca gcagatcagc cacatcgccc agcagcttgg gctcgagaag
11641 gatgtggtcc gagtgtggtt ctgtaaccgg cgccagaagg gcaagcgatc aagcagcgac
11701 tatgcacaac gagaggattt tgaggctgct gggtctcctt tctcaggggg accagtgtcc
11761 tttcctctgg ccccagggcc ccattttggt accccaggct atgggagccc tcacttcact
11821 gcactgtact cctcggtccc tttccctgag ggggaagcct ttccccctgt ctccgtcacc
11881 actctgggct ctcccatgca ttcaaacgga agtggcgtga aacagacttt gaattttgac
11941 cttctcaagt tggcgggaga cgtggagtcc aacccagggc ccatggctgt cagcgacgcg
12001 ctgctcccat ctttctccac gttcgcgtct ggcccggcgg gaagggagaa gacactgcgt
12061 caagcaggtg ccccgaataa ccgctggcgg gaggagctct cccacatgaa gcgacttccc
12121 ccagtgcttc ccggccgccc ctatgacctg gcggcggcga ccgtggccac agacctggag
12181 agcggcggag ccggtgcggc ttgcggcggt agcaacctgg cgcccctacc tcggagagag
12241 accgaggagt tcaacgatct cctggacctg gactttattc tctccaattc gctgacccat
12301 cctccggagt cagtggccgc caccgtgtcc tcgtcagcgt cagcctcctc ttcgtcgtcg
12361 ccgtcgagca gcggccctgc cagcgcgccc tccacctgca gcttcaccta tccgatccgg
12421 gccgggaacg acccgggcgt ggcgccgggc ggcacgggcg gaggcctcct ctatggcagg
12481 gagtccgctc cccctccgac ggctcccttc aacctggcgg acatcaacga cgtgagcccc
12541 tcgggcggct tcgtggccga gctcctgcgg ccagaattgg acccggtgta cattccgccg
12601 cagcagccgc agccgccagg tggcgggctg atgggcaagt tcgtgctgaa ggcgtcgctg
12661 agcgcccctg gcagcgagta cggcagcccg tcggtcatca gcgtcagcaa aggcagccct
12721 gacggcagcc acccggtggt ggtggcgccc tacaacggcg ggccgccgcg cacgtgcccc
12781 aagatcaagc aggaggcggt ctcttcgtgc acccacttgg gcgctggacc ccctctcagc
12841 aatggccacc ggccggctgc acacgacttc cccctggggc ggcagctccc cagcaggact
12901 accccgaccc tgggtcttga ggaagtgctg agcagcaggg actgtcaccc tgccctgccg
12961 cttcctcccg gcttccatcc ccacccgggg cccaattacc catccttcct gcccgatcag
13021 atgcagccgc aagtcccgcc gctccattac caagagctca tgccacccgg ttcctgcatg
13081 ccagaggagc ccaagccaaa gaggggaaga cgatcgtggc cccggaaaag gaccgccacc
13141 cacacttgtg attacgcggg ctgcggcaaa acctacacaa agagttccca tctcaaggca
13201 cacctgcgaa cccacacagg tgagaaacct taccactgtg actgggacgg ctgtggatgg
13261 aaattcgccc gctcagatga actgaccagg cactaccgta aacacacggg gcaccgcccg
13321 ttccagtgcc aaaaatgcga ccgagcattt tccaggtcgg accacctcgc cttacacatg
13381 aagaggcatt ttggcagcgg cgagggcagg ggcagcctgc tgacctgcgg cgacgtggag
13441 gagaaccccg gccccatgta caacatgatg gagacggagc tgaagccgcc gggcccgcag
13501 caaacttcgg ggggcggcgg cggcaactcc accgcggcgg cggccggcgg caaccagaaa
13561 aacagcccgg accgcgtcaa gcggcccatg aatgccttca tggtgtggtc ccgcgggcag
13621 cggcgcaaga tggcccagga gaaccccaag atgcacaact cggagatcag caagcgcctg
13681 ggcgccgagt ggaaactttt gtcggagacg gagaagcggc cgttcatcga cgaggctaag
13741 cggctgcgag cgctgcacat gaaggagcac ccggattata aataccggcc ccggcggaaa
13801 accaagacgc tcatgaagaa ggataagtac acgctgcccg gcgggctgct ggcccccggc
13861 ggcaatagca tggcgagcgg ggtcggggtg ggcgccggcc tgggcgcggg cgtgaaccag
13921 cgcatggaca gttacgcgca catgaacggc tggagcaacg gcagctacag catgatgcag
13981 gaccagctgg gctacccgca gcacccgggc ctcaatgcgc acggcgcagc gcagatgcag
14041 cccatgcacc gctacgacgt gagcgccctg cagtacaact ccatgaccag ctcgcagacc
14101 tacatgaacg gctcgcccac ctacagcatg tcctactcgc agcagggcac ccctggcatg
14161 gctcttggct ccatgggttc ggtggtcaag tccgaggcca gctccagccc ccctgtggtt
14221 acctcttcct cccactccag ggcgccctgc caggccgggg acctccggga catgatcagc
14281 atgtatctcc ccggcgccga ggtgccggaa cccgccgccc ccagcagact tcacatgtcc
14341 cagcactacc agagcggccc ggtgcccggc acggccatta acggcacact gcccctctca
14401 cacatgggta gtgggcaatg tactaactac gctttgttga aactcgctgg cgatgttgaa
14461 agtaaccccg gtcctatgcc cctcaacgtt agcttcacca acaggaacta tgacctcgac
14521 tacgactcgg tgcagccgta tttctactgc gacgaggagg agaacttcta ccagcagcag
14581 cagcagagcg agctgcagcc cccggcgccc agcgaggata tctggaagaa attcgagctg
14641 ctgcccaccc cgcccctgtc ccctagccgc cgctccgggc tctgctcgcc ctcctacgtt
14701 gcggtcacac ccttctccct tcggggagac aacgacggcg gtggcgggag cttctccacg
14761 gccgaccagc tggagatggt gaccgagctg ctgggaggag acatggtgaa ccagagtttc
14821 atctgcgacc cggacgacga gaccttcatc aaaaacatca tcatccagga ctgtatgtgg
14881 agcggcttct cggccgccgc caagctcgtc tcagagaagc tggcctccta ccaggctgcg
14941 cgcaaagaca gcggcagccc gaaccccgcc cgcggccaca gcgtctgctc cacctccagc
15001 ttgtacctgc aggatctgag cgccgccgcc tcagagtgca tcgacccctc ggtggtcttc
15061 ccctaccctc tcaacgacag cagctcgccc aagtcctgcg cctcgcaaga ctccagcgcc
15121 ttctctccgt cctcggattc tctgctctcc tcgacggagt cctccccgca gggcagcccc
15181 gagcccctgg tgctccatga ggagacaccg cccaccacca gcagcgactc tgaggaggaa
15241 caagaagatg aggaagaaat cgatgttgtt tctgtggaaa agaggcaggc tcctggcaaa
15301 aggtcagagt ctggatcacc ttctgctgga ggccacagca aacctcctca cagcccactg
15361 gtcctcaaga ggtgccacgt ctccacacat cagcacaact acgcagcgcc tccctccact
15421 cggaaggact atcctgctgc caagagggtc aagttggaca gtgtcagagt cctgagacag
15481 atcagcaaca accgaaaatg caccagcccc aggtcctcgg acaccgagga gaatgtcaag
15541 aggcgaacac acaacgtctt ggagcgccag aggaggaacg agctaaaacg gagctttttt
15601 gccctgcgtg accagatccc ggagttggaa aacaatgaaa aggcccccaa ggtagttatc
15661 cttaaaaaag ccacagcata catcctgtcc gtccaagcag aggagcaaaa gctcatttct
15721 gaagaggact tgttgcggaa acgacgagaa cagttgaaac acaaacttga acagctacgg
15781 aactcttgtg cgtaatagcg gccgcgactc tagatcataa tcagccatac cacatttgta
15841 gaggttttac ttgctttaaa aaacctccca cacctccccc tgaacctgaa acataaaatg
15901 aatgcaattg ttgttgttaa cttgtttatt gcagcttata atggttacaa ataaagcaat
15961 agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc
16021 aaactcatca atgtatctta aggcgtaaat tgtaagcgtt aatattttgt taaaattcgc
16081 gttaaatttt tgttaaatca gctcattttt taaccaatag gccgaaatcg gcaaaatccc
16141 ttataaatca aaagaataga ccgagatagg gttgagtgtt gttccagttt ggaacaagag
16201 tccactatta aagaacgtgg actccaacgt caaagggcga aaaaccgtct atcagggcga
16261 tggcccacta cgtgaaccat caccctaatc aagttttttg gggtcgaggt gccgtaaagc
16321 actaaatcgg aaccctaaag ggagcccccg atttagagct tgacggggaa agccggcgaa
16381 cgtggcgaga aaggaaggga agaaagcgaa aggagcgggc gctagggcgc tggcaagtgt
16441 agcggtcacg ctgcgcgtaa ccaccacacc cgccgcgctt aatgcgccgc tacagggcgc
16501 gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat
16561 acagcgctag ttattaatag taatcaatta cggggtcatt agttcatagc ccatatatgg
16621 agttccgcgt tacataactt acggtaaatg gcccgcctgg ctgaccgccc aacgaccccc
16681 gcccattgac gtcaataatg acgtatgttc ccatagtaac gccaataggg actttccatt
16741 gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt ggcagtacat caagtgtatc
16801 atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg
16861 cccagtacat gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg
16921 ctattaccat ggtcgaggtg agccccacgt tctgcttcac tctccccatc tcccccccct
16981 ccccaccccc aattttgtat ttatttattt tttaattatt ttgtgcagcg atgggggcgg
17041 gggggggggg ggggcgcgcg ccaggcgggg cggggcgggg cgaggggcgg ggcggggcga
17101 ggcggagagg tgcggcggca gccaatcaga gcggcgcgct ccgaaagttt ccttttatgg
17161 cgaggcggcg gcggcggcgg ccctataaaa agcgaagcgc gcggcgggcg ggagtcgctg
17221 cgcgctgcct tcgccccgtg ccccgctccg ccgccgcctc gcgccgcccg ccccggctct
17281 gactgaccgc gttactccca caggtgagcg ggcgggacgg cccttctcct ccgggctgta
17341 attagcgctt ggtttaatga cggcttgttt cttttctgtg gctgcgtgaa agccttgagg
17401 ggctccggga gggccctttg tgcgggggga gcggctcggg gggtgcgtgc gtgtgtgtgt
17461 gcgtggggag cgccgcgtgc ggctccgcgc tgcccggcgg ctgtgagcgc tgcgggcgcg
17521 gcgcggggct ttgtgcgctc cgcagtgtgc gcgaggggag cgcggccggg ggcggtgccc
17581 cgcggtgcgg ggggggctgc gaggggaaca aaggctgcgt gcggggtgtg tgcgtggggg
17641 ggtgagcagg gggtgtgggc gcgtcggtcg ggctgcaacc ccccctgcac ccccctcccc
17701 gagttgctga gcacggcccg gcttcgggtg cggggctccg tacggggcgt ggcgcggggc
17761 tcgccgtgcc gggcgggggg tggcggcagg tgggggtgcc gggcggggcg gggccgcctc
17821 gggccgggga gggctcgggg gaggggcgcg gcggcccccg gagcgccggc ggctgtcgag
17881 gcgcggcgag ccgcagccat tgccttttat ggtaatcgtg cgagagggcg cagggacttc
17941 ctttgtccca aatctgtgcg gagccgaaat ctgggaggcg ccgccgcacc ccctctagcg
18001 ggcgcggggc gaagcggtgc ggcgccggca ggaaggaaat gggcggggag ggccttcgtg
18061 cgtcgccgcg ccgccgtccc cttctccctc tccagcctcg gggctgtccg cggggggacg
18121 gctgccttcg ggggggacgg ggcagggcgg ggttcggctt ctggcgtgtg accggcggct
18181 ctagagcctc tgctaaccat gttcatgcct tcttcttttt cctacagctc ctgggcaacg
18241 tgctggttat tgtgctgtct catcattttg gcaaagaatt ctagggataa cagggtaata
18301 tggcttcgta ccccggccat caacacgcgt ctgcgttcga ccaggctgcg cgttctcgcg
18361 gccatagcaa ccgacgtacg gcgttgcgcc ctcgccggca gcaagaagcc acggaagtcc
18421 gcccggagca gaaaatgccc acgctactgc gggtttatat agacggtccc cacgggatgg
18481 ggaaaaccac caccacgcaa ctgctggtgg ccctgggttc gcgcgacgat atcgtctacg
18541 tacccgagcc gatgacttac tggcgggtgc tgggggcttc cgagacaatc gcgaacatct
18601 acaccacaca acaccgcctc gaccagggtg agatatcggc cggggacgcg gcggtggtaa
18661 tgacaagcgc ccagataaca atgggcatgc cttatgccgt gaccgacgcc gttctggctc
18721 ctcatatcgg gggggaggct gggagctcac atgccccgcc cccggccctc accctcatct
18781 tcgaccgcca tcccatcgcc gccctcctgt gctacccggc cgcgcggtac cttatgggca
18841 gcatgacccc ccaggccgtg ctggcgttcg tggccctcat cccgccgacc ttgcccggca
18901 ccaacatcgt gcttggggcc cttccggagg acagacacat cgaccgcctg gccaaacgcc
18961 agcgccccgg cgagcggctg gacctggcta tgctggctgc gattcgccgc gtttacgggc
19021 tacttgccaa tacggtgcgg tatctgcagt gcggcgggtc gtggcgggag gactggggac
19081 agctttcggg gacggccgtg ccgccccagg gtgccgagcc ccagagcaac gcgggcccac
19141 gaccccatat cggggacacg ttatttaccc tgtttcgggc ccccgagttg ctggccccca
19201 acggcgacct gtataacgtg tttgcctggg ccttggacgt cttggccaaa cgcctccgtt
19261 ccatgcacgt ctttatcctg gattacgacc aatcgcccgc cggctgccgg gacgccctgc
19321 tgcaacttac ctccgggatg gtccagaccc acgtcaccac ccccggctcc ataccgacga
19381 tatgcgacct ggcgcgcacg tttgcccggg agatggggga ggctaactga gcggccgcgg
19441 atccgcccct ctccctcccc cccccctaac gttactggcc gaagccgctt ggaataaggc
19501 cggtgtgcgt ttgtctatat gttattttcc accatattgc cgtcttttgg caatgtgagg
19561 gcccggaaac ctggccctgt cttcttgacg agcattccta ggggtctttc ccctctcgcc
19621 aaaggaatgc aaggtctgtt gaatgtcgtg aaggaagcag ttcctctgga agcttcttga
19681 agacaaacaa cgtctgtagc gaccctttgc aggcagcgga accccccacc tggcgacagg
19741 tgcctctgcg gccaaaagcc acgtgtataa gatacacctg caaaggcggc acaaccccag
19801 tgccacgttg tgagttggat agttgtggaa agagtcaaat ggctctcctc aagcgtattc
19861 aacaaggggc tgaaggatgc ccagaaggta ccccattgta tgggatctga tctggggcct
19921 cggtgcacat gctttacatg tgtttagtcg aggttaaaaa aacgtctagg ccccccgaac
19981 cacggggacg tggttttcct ttgaaaaaca cgatgataat atggccacaa ccatgggatc
20041 cgccattgaa caagatggat tgcacgcagg ttctccggcc gcttgggtgg agaggctatt
20101 cggctatgac tgggcacaac agacaatcgg ctgctctgat gccgccgtgt tccggctgtc
20161 agcgcagggg cgcccggttc tttttgtcaa gaccgacctg tccggtgccc tgaatgaact
20221 gcaggacgag gcagcgcggc tatcgtggct ggccacgacg ggcgttcctt gcgcagctgt
20281 gctcgacgtt gtcactgaag cgggaaggga ctggctgcta ttgggcgaag tgccggggca
20341 ggatctcctg tcatctcacc ttgctcctgc cgagaaagta tccatcatgg ctgatgcaat
20401 gcggcggctg catacgcttg atccggctac ctgcccattc gaccaccaag cgaaacatcg
20461 catcgagcga gcacgtactc ggatggaagc cggtcttgtc gatcaggatg atctggacga
20521 agagcatcag gggctcgcgc cagccgaact gttcgccagg ctcaaggcgc gcatgcccga
20581 cggcgaggat ctcgtcgtga cccatggcga tgcctgcttg ccgaatatca tggtggaaaa
20641 tggccgcttt tctggattca tcgactgtgg ccggctgggt gtggcggacc gctatcagga
20701 catagcgttg gctacccgtg atattgctga agagcttggc ggcgaatggg ctgaccgctt
20761 cctcgtgctt tacggtatcg ccgctcccga ttcgcagcgc atcgccttct atcgccttct
20821 tgacgagttc ttctgagcgg gactctgggg ttcgaaatga ccgaccaagc gacgcccaac
20881 ctgccatcac gagatttcga ttccaccgcc gccttctatg aaaggttggg cttcggaatc
20941 gttttccggg acgccggctg gatgatcctc cagcgcgggg atctcatgct ggagttcttc
21001 gcccacccta gggggaggct aactgaaaca cggaaggaga caataccgga aggaacccgc
21061 gctatgacgg caataaaaag acagaataaa acgcacggtg ttgggtcgtt tgttcggcgc
21121 ggcgattaat cgtgaggctc cggtgcccgt cagtgggcag agcgcacatc gcccacagtc
21181 cccgagaagt tggggggagg ggtcggcaat tgaaccggtg cctagagaag gtggcgcggg
21241 gtaaactggg aaagtgatgt cgtgtactgg ctccgccttt ttcccgaggg tgggggagaa
21301 ccgtatataa gtgcagtagt cgccgtgaac gttctttttc gcaacgggtt tgccgccaga
21361 acacaggtaa gtgccgtgtg tggttcccgc gggcctggcc tctttacggg ttatggccct
21421 tgcgtgcctt gaattacttc cacctggctg cagtacgtga ttcttgatcc cgagcttcgg
21481 gttggaagtg ggtgggagag ttcgaggcct tgcgcttaag gagccccttc gcctcgtgct
21541 tgagttgagg cctggcctgg gcgctggggc cgccgcgtgc gaatctggtg gcaccttcgc
21601 gcctgtctcg ctgctttcga taagtctcta gccatttaaa atttttgatg acctgctgcg
21661 acgctttttt tctggcaaga tagtcttgta aatgcgggcc aagatctgca cactggtatt
21721 tcggtttttg gggccgcggg cggcgacggg gcccgtgcgt cccagcgcac atgttcggcg
21781 aggcggggcc tgcgagcgcg gccaccgaga atcggacggg ggtagtctca agctggccgg
21841 cctgctctgg tgcctggcct cgcgccgccg tgtatcgccc cgccctgggc ggcaaggctg
21901 gcccggtcgg caccagttgc gtgagcggaa agatggccgc ttcccggccc tgctgcaggg
21961 agctcaaaat ggaggacgcg gcgctcggga gagcgggcgg gtgagtcacc cacacaaagg
22021 aaaagggcct ttccgtcctc agccgtcgct tcatgtgact ccacggagta ccgggcgccg
22081 tccaggcacc tcgattagtt ctcgagcttt tggagtacgt cgtctttagg ttggggggag
22141 gggttttatg cgatggagtt tccccacact gagtgggtgg agactgaagt taggccagct
22201 tggcacttga tgtaattctc cttggaattt gccctttttg agtttggatc ttggttcatt
22261 ctcaagcctc agacagtggt tcaaagtttt tttcttccat ttcaggtgtc gtgaagctag
22321 cgatcgccac catgtcttct aattcagata ctggggattt acaagagtct ttaaagcacg
22381 gacttacacc tattgtgtct caatttaaaa tggtgaatta ctcctatgat gaagatctgg
22441 aagagctttg tcccgtgtgt ggagataaag tgtctgggta ccattatggg ctcctcacct
22501 gtgaaagctg caagggattt tttaagcgaa cagtccaaaa taataaaagg tacacatgta
22561 tagaaaacca gaactgccaa attgacaaaa cacagagaaa gcgttgtcct tactgtcgtt
22621 ttcaaaaatg tctaagtgtt ggaatgaagc tagaagctgt aagggccgac cgaatgcgtg
22681 gaggaaggaa taagtttggg ccaatgtaca agagagacag ggccctgaag caacagaaaa
22741 aagccctcat ccgagccaat ggacttaagc tagaagccat gtctcaggtg atccaagcta
22801 tgccctctga cctgaccatt tcctctgcaa ttcaaaacat ccactctgcc tccaaaggcc
22861 tacctctgaa ccatgctgcc ttgcctccta cagactatga cagaagtccc tttgtaacat
22921 cccccattag catgacaatg ccccctcacg gcagcctgca aggttaccaa acatatggcc
22981 actttcctag ccgggccatc aagtctgagt acccagaccc ctataccagc tcacccgagt
23041 ccataatggg ctattcatat atggatagtt accagacgag ctctccagca agcatcccac
23101 atctgatact ggaacttttg aagtgtgagc cagatgagcc tcaagtccag gctaaaatca
23161 tggcctattt gcagcaagag caggctaacc gaagcaagca cgaaaagctg agcacctttg
23221 ggcttatgtg caaaatggca gatcaaactc tcttctccat tgtcgagtgg gccaggagta
23281 gtatcttctt cagagaactt aaggttgatg accaaatgaa gctgcttcag aactgctgga
23341 gtgagctctt aatcctcgac cacatttacc gacaagtggt acatggaaag gaaggatcca
23401 tcttcctggt tactgggcaa caagtggact attccataat agcatcacaa gccggagcca
23461 ccctcaacaa cctcatgagt catgcacagg agttagtggc aaaacttcgt tctctccagt
23521 ttgatcaacg agagttcgta tgtctgaaat tcttggtgct ctttagttta gatgtcaaaa
23581 accttgaaaa cttccagctg gtagaaggtg tccaggaaca agtcaatgcc gccctgctgg
23641 actacacaat gtgtaactac ccgcagcaga cagagaaatt tggacagcta cttcttcgac
23701 tacccgaaat ccgggccatc agtatgcagg ctgaagaata cctctactac aagcacctga
23761 acggggatgt gccctataat aaccttctca ttgaaatgtt gcatgccaaa agagcataaa
23821 tttaaatgtc gacgcggccg cgggactctg gggttcgaaa tgaccgacca agcgacgccc
23881 aacctgccat cacgagattt cgattccacc gccgccttct atgaaaggtt gggcttcgga
23941 atcgttttcc gggacgccgg ctggatgatc ctccagcgcg gggatctcat gctggagttc
24001 ttcgcccacc ctagggggag gctaactgaa acacggaagg agacaatacc ggaaggaacc
24061 cgcgctatga cggcaataaa aagacagaat aaaacgcacg gtgttgggtc gtttgttcgg
24121 cgcgccctcg acattgatta ttgactagtt at
//
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