pMaster3

LOCUS       pMaster3              24152 bp ds-DNA     circular SYN 12-MAY-2021
DEFINITION  Reprogram somatic cells to iPS cells.
ACCESSION   .
VERSION     .
KEYWORDS    pMaster3
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 24152)
  AUTHORS   Wu S, Wu Y, Zhang X, Capecchi MR
  TITLE     Efficient germ-line transmission obtained with transgene-free 
            induced pluripotent stem cells.
  JOURNAL   Proc Natl Acad Sci U S A. 2014 Jul 22;111(29):10678-83. doi: 
            10.1073/pnas.1409933111. Epub 2014 Jul 7.
  PUBMED    25002522
REFERENCE   2  (bases 1 to 24152)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 12, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..24152
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        359..636
                     /label=chicken beta-actin promoter
     intron          637..1653
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     CDS             2646..2711
                     /codon_start=1
                     /product="2A peptide from foot-and-mouth disease virus 
                     polyprotein"
                     /label=F2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="VKQTLNFDLLKLAGDVESNPGP"
     rep_origin      complement(3523..4111)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3603..3622)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(4282..5142)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4905..4924
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5143..5247)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     5315..5333
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     rep_origin      7371..9160
                     /label=oriP
                     /note="Epstein-Barr virus oriP replication origin (Yates et
                     al., 2000)"
     promoter        9636..10814
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          9867..10805
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     regulatory      10822..10831
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     CDS             11917..11982
                     /codon_start=1
                     /product="2A peptide from foot-and-mouth disease virus 
                     polyprotein"
                     /label=F2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="VKQTLNFDLLKLAGDVESNPGP"
     CDS             13402..13455
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             14416..14475
                     /codon_start=1
                     /product="2A peptide from equine rhinitis A virus 
                     polyprotein"
                     /label=E2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="QCTNYALLKLAGDVESNPGP"
     CDS             14476..15795
                     /codon_start=1
                     /product="human c-Myc proto-oncogene"
                     /label=c-Myc
                     /translation="MPLNVSFTNRNYDLDYDSVQPYFYCDEEENFYQQQQQSELQPPAP
                     SEDIWKKFELLPTPPLSPSRRSGLCSPSYVAVTPFSLRGDNDGGGGSFSTADQLEMVTE
                     LLGGDMVNQSFICDPDDETFIKNIIIQDCMWSGFSAAAKLVSEKLASYQAARKDSGSPN
                     PARGHSVCSTSSLYLQDLSAAASECIDPSVVFPYPLNDSSSPKSCASQDSSAFSPSSDS
                     LLSSTESSPQGSPEPLVLHEETPPTTSSDSEEEQEDEEEIDVVSVEKRQAPGKRSESGS
                     PSAGGHSKPPHSPLVLKRCHVSTHQHNYAAPPSTRKDYPAAKRVKLDSVRVLRQISNNR
                     KCTSPRSSDTEENVKRRTHNVLERQRRNELKRSFFALRDQIPELENNEKAPKVVILKKA
                     TAYILSVQAEEQKLISEEDLLRKRREQLKHKLEQLRNSCA"
     polyA_signal    15919..16040
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(15956..15975)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     16010..16029
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(16047..16502)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(16184..16205)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     16396..16415
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     enhancer        16628..16931
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        16933..17210
                     /label=chicken beta-actin promoter
     intron          17211..18227
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     CDS             18300..19430
                     /codon_start=1
                     /product="herpes simplex virus thymidine kinase"
                     /label=HSV TK
                     /note="confers sensitivity to nucleoside analogs such as 
                     acyclovir or ganciclovir"
                     /translation="MASYPGHQHASAFDQAARSRGHSNRRTALRPRRQQEATEVRPEQK
                     MPTLLRVYIDGPHGMGKTTTTQLLVALGSRDDIVYVPEPMTYWRVLGASETIANIYTTQ
                     HRLDQGEISAGDAAVVMTSAQITMGMPYAVTDAVLAPHIGGEAGSSHAPPPALTLIFDR
                     HPIAALLCYPAARYLMGSMTPQAVLAFVALIPPTLPGTNIVLGALPEDRHIDRLAKRQR
                     PGERLDLAMLAAIRRVYGLLANTVRYLQCGGSWREDWGQLSGTAVPPQGAEPQSNAGPR
                     PHIGDTLFTLFRAPELLAPNGDLYNVFAWALDVLAKRLRSMHVFILDYDQSPAGCRDAL
                     LQLTSGMVQTHVTTPGSIPTICDLARTFAREMGEAN"
     misc_feature    19446..20032
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(19613..19630)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     19840..19859
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             20033..20836
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MGSAIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQ
                     GRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQD
                     LLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                     EHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQ
                     DIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(20096..20115)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     20706..20725
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    21068..21115
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     promoter        21136..22314
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          21367..22305
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     polyA_signal    24071..24118
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     enhancer        24130..357
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 taatagtaat caattacggg gtcattagtt catagcccat atatggagtt ccgcgttaca
       61 taacttacgg taaatggccc gcctggctga ccgcccaacg acccccgccc attgacgtca
      121 ataatgacgt atgttcccat agtaacgcca atagggactt tccattgacg tcaatgggtg
      181 gagtatttac ggtaaactgc ccacttggca gtacatcaag tgtatcatat gccaagtacg
      241 ccccctattg acgtcaatga cggtaaatgg cccgcctggc attatgccca gtacatgacc
      301 ttatgggact ttcctacttg gcagtacatc tacgtattag tcatcgctat taccatggtc
      361 gaggtgagcc ccacgttctg cttcactctc cccatctccc ccccctcccc acccccaatt
      421 ttgtatttat ttatttttta attattttgt gcagcgatgg gggcgggggg gggggggggg
      481 cgcgcgccag gcggggcggg gcggggcgag gggcggggcg gggcgaggcg gagaggtgcg
      541 gcggcagcca atcagagcgg cgcgctccga aagtttcctt ttatggcgag gcggcggcgg
      601 cggcggccct ataaaaagcg aagcgcgcgg cgggcgggag tcgctgcgcg ctgccttcgc
      661 cccgtgcccc gctccgccgc cgcctcgcgc cgcccgcccc ggctctgact gaccgcgtta
      721 ctcccacagg tgagcgggcg ggacggccct tctcctccgg gctgtaatta gcgcttggtt
      781 taatgacggc ttgtttcttt tctgtggctg cgtgaaagcc ttgaggggct ccgggagggc
      841 cctttgtgcg gggggagcgg ctcggggggt gcgtgcgtgt gtgtgtgcgt ggggagcgcc
      901 gcgtgcggct ccgcgctgcc cggcggctgt gagcgctgcg ggcgcggcgc ggggctttgt
      961 gcgctccgca gtgtgcgcga ggggagcgcg gccgggggcg gtgccccgcg gtgcgggggg
     1021 ggctgcgagg ggaacaaagg ctgcgtgcgg ggtgtgtgcg tgggggggtg agcagggggt
     1081 gtgggcgcgt cggtcgggct gcaacccccc ctgcaccccc ctccccgagt tgctgagcac
     1141 ggcccggctt cgggtgcggg gctccgtacg gggcgtggcg cggggctcgc cgtgccgggc
     1201 ggggggtggc ggcaggtggg ggtgccgggc ggggcggggc cgcctcgggc cggggagggc
     1261 tcgggggagg ggcgcggcgg cccccggagc gccggcggct gtcgaggcgc ggcgagccgc
     1321 agccattgcc ttttatggta atcgtgcgag agggcgcagg gacttccttt gtcccaaatc
     1381 tgtgcggagc cgaaatctgg gaggcgccgc cgcaccccct ctagcgggcg cggggcgaag
     1441 cggtgcggcg ccggcaggaa ggaaatgggc ggggagggcc ttcgtgcgtc gccgcgccgc
     1501 cgtccccttc tccctctcca gcctcggggc tgtccgcggg gggacggctg ccttcggggg
     1561 ggacggggca gggcggggtt cggcttctgg cgtgtgaccg gcggctctag agcctctgct
     1621 aaccatgttc atgccttctt ctttttccta cagctcctgg gcaacgtgct ggttattgtg
     1681 ctgtctcatc attttggcaa agaattcgcg attaagccac catgagtgtg gatccagctt
     1741 gtccccaaag cttgccttgc tttgaagcat ccgactgtaa agaatcttca cctatgcctg
     1801 tgatttgtgg gcctgaagaa aactatccat ccttgcaaat gtcttctgct gagatgcctc
     1861 acacggagac tgtctctcct cttccttcct ccatggatct gcttattcag gacagccctg
     1921 attcttccac cagtcccaaa ggcaaacaac ccacttctgc agagaagagt gtcgcaaaaa
     1981 aggaagacaa ggtcccggtc aagaaacaga agaccagaac tgtgttctct tccacccagc
     2041 tgtgtgtact caatgataga tttcagagac agaaatacct cagcctccag cagatgcaag
     2101 aactctccaa catcctgaac ctcagctaca aacaggtgaa gacctggttc cagaaccaga
     2161 gaatgaaatc taagaggtgg cagaaaaaca actggccgaa gaatagcaat ggtgtgacgc
     2221 agaaggcctc agcacctacc taccccagcc tttactcttc ctaccaccag ggatgcctgg
     2281 tgaacccgac tgggaacctt ccaatgtgga gcaaccagac ctggaacaat tcaacctgga
     2341 gcaaccagac ccagaacatc cagtcctgga gcaaccactc ctggaacact cagacctggt
     2401 gcacccaatc ctggaacaat caggcctgga acagtccctt ctataactgt ggagaggaat
     2461 ctctgcagtc ctgcatgcag ttccagccaa attctcctgc cagtgacttg gaggctgcct
     2521 tggaagctgc tggggaaggc cttaatgtaa tacagcagac cactaggtat tttagtactc
     2581 cacaaaccat ggatttattc ctaaactact ccatgaacat gcaacctgaa gacgtgggaa
     2641 gtggcgtgaa acagactttg aattttgacc ttctcaagtt ggcgggagac gtggagtcca
     2701 acccagggcc catgggctcc gtgtccaacc agcagtttgc aggtggctgc gccaaggcgg
     2761 cagaagaggc gcccgaggag gcgccggagg acgcggcccg ggcggcggac gagcctcagc
     2821 tgctgcacgg tgcgggcatc tgtaagtggt tcaacgtgcg catggggttc ggcttcctgt
     2881 ccatgaccgc ccgcgccggg gtcgcgctcg accccccagt ggatgtcttt gtgcaccaga
     2941 gtaagctgca catggaaggg ttccggagct tgaaggaggg tgaggcagtg gagttcacct
     3001 ttaagaagtc agccaagggt ctggaatcca tccgtgtcac cggacctggt ggagtattct
     3061 gtattgggag tgagaggcgg ccaaaaggaa agagcatgca gaagcgcaga tcaaaaggag
     3121 acaggtgcta caactgtgga ggtctagatc atcatgccaa ggaatgcaag ctgccacccc
     3181 agcccaagaa gtgccacttc tgccagagca tcagccatat ggtagcctca tgtccgctga
     3241 aggcccagca gggccctagt gcacagggaa agccaaccta ctttcgagag gaagaagaag
     3301 aaatccacag ccctaccctg ctcccggagg cacagaattg ataagataaa cgtcgacgcg
     3361 aggctggatg gccttcccca ttatgattct tctcgcttcc ggcggcatcg ggatgcccgc
     3421 gttgcaggcc atgctgtcca ggcaggtaga tgacgaccat cagggacagc ttcaaggcca
     3481 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc
     3541 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact
     3601 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct
     3661 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag
     3721 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca
     3781 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa
     3841 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc
     3901 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag
     3961 aaggacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg
     4021 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca
     4081 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc
     4141 tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag
     4201 gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata
     4261 tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat
     4321 ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg
     4381 ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc
     4441 tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc
     4501 aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc
     4561 gccagttaat agtttgcgca acgttgttgc cattgctgca ggcatcgtgg tgtcacgctc
     4621 gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc
     4681 ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa
     4741 gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat
     4801 gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata
     4861 gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaaca cgggataata ccgcgccaca
     4921 tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag
     4981 gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc
     5041 agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc
     5101 aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata
     5161 ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta
     5221 gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtcta
     5281 agaaaccatt attatcatga cattaaccta taaaaatagg cgtatcacga ggccctttcg
     5341 tcttcaagaa ttctcatgtt tgacagctta tcatcgataa gctgatcctc acaggccgca
     5401 cccagctttt cttccgttgc cccagtagca tctctgtctg gtgaccttga agaggaagag
     5461 gaggggtccc gagaatcccc atccctaccg tccagcaaaa agggggacga ggaatttgag
     5521 gcctggcttg aggctcagga cgcaaatctt gaggatgttc agcgggagtt ttccgggctg
     5581 cgagtaattg gtgatgagga cgaggatggt tcggaggatg gggaattttc agacctggat
     5641 ctgtctgaca gcgaccatga aggggatgag ggtggggggg ctgttggagg gggcaggagt
     5701 ctgcactccc tgtattcact gagcgtcgtc taataaagat gtctattgat ctcttttagt
     5761 gtgaatcatg tctgacgagg ggccaggtac aggacctgga aatggcctag gagagaaggg
     5821 agacacatct ggaccagaag gctccggcgg cagtggacct caaagaagag ggggtgataa
     5881 ccatggacga ggacggggaa gaggacgagg acgaggaggc ggaagaccag gagccccggg
     5941 cggctcagga tcagggccaa gacatagaga tggtgtccgg agaccccaaa aacgtccaag
     6001 ttgcattggc tgcaaaggga cccacggtgg aacaggagca ggagcaggag cgggaggggc
     6061 aggagcagga ggggcaggag caggaggagg ggcaggagca ggaggggcag gagcaggagg
     6121 tggaggccgg ggtcgaggag gcagtggagg ccggggtcga ggaggtagtg gaggccgggg
     6181 tcgaggaggt agtggaggcc gccggggtag aggacgtgaa agagccaggg ggggaagtcg
     6241 tgaaagagcc agggggagag gtcgtggacg tggagaaaag aggcccagga gtcccagtag
     6301 tcagtcatca tcatccgggt ctccaccgcg caggccccct ccaggtagaa ggccattttt
     6361 ccaccctgta ggggaagccg attattttga ataccaccaa gaaggtggcc cagatggtga
     6421 gcctgacgtg cccccgggag cgatagagca gggccccgca gatgacccag gagaaggccc
     6481 aagcactgga ccccggggtc agggtgatgg aggcaggcgc aaaaaaggag ggtggtttgg
     6541 aaagcatcgt ggtcaaggag gttccaaccc gaaatttgag aacattgcag aaggtttaag
     6601 agctctcctg gctaggagtc acgtagaaag gactaccgac gaaggaactt gggtcgccgg
     6661 tgtgttcgta tatggaggta gtaagacctc cctttacaac ctaaggcgag gaactgccct
     6721 tgctattcca caatgtcgtc ttacaccatt gagtcgtctc ccctttggaa tggcccctgg
     6781 acccggccca caacctggcc cgctaaggga gtccattgtc tgttatttca tggtcttttt
     6841 acaaactcat atatttgctg aggttttgaa ggatgcgatt aaggaccttg ttatgacaaa
     6901 gcccgctcct acctgcaata tcagggtgac tgtgtgcagc tttgacgatg gagtagattt
     6961 gcctccctgg tttccaccta tggtggaagg ggctgccgcg gagggtgatg acggagatga
     7021 cggagatgaa ggaggtgatg gagatgaggg tgaggaaggg caggagtgat gtaacttgtt
     7081 aggagacgcc ctcaatcgta ttaaaagccg tgtattcccc cgcactaaag aataaatccc
     7141 cagtagacat catgcgtgct gttggtgtat ttctggccat ctgtcttgtc accattttcg
     7201 tcctcccaac atggggcaat tgggcatacc catgttgtca cgtcactcag ctccgcgctc
     7261 aacaccttct cgcgttggaa aacattagcg acatttacct ggtgagcaat cagacatgcg
     7321 acggctttag cctggcctcc ttaaattcac ctaagaatgg gagcaaccag caggaaaagg
     7381 acaagcagcg aaaattcacg cccccttggg aggtggcggc atatgcaaag gatagcactc
     7441 ccactctact actgggtatc atatgctgac tgtatatgca tgaggatagc atatgctacc
     7501 cggatacaga ttaggatagc atatactacc cagatataga ttaggatagc atatgctacc
     7561 cagatataga ttaggatagc ctatgctacc cagatataaa ttaggatagc atatactacc
     7621 cagatataga ttaggatagc atatgctacc cagatataga ttaggatagc ctatgctacc
     7681 cagatataga ttaggatagc atatgctacc cagatataga ttaggatagc atatgctatc
     7741 cagatatttg ggtagtatat gctacccaga tataaattag gatagcatat actaccctaa
     7801 tctctattag gatagcatat gctacccgga tacagattag gatagcatat actacccaga
     7861 tatagattag gatagcatat gctacccaga tatagattag gatagcctat gctacccaga
     7921 tataaattag gatagcatat actacccaga tatagattag gatagcatat gctacccaga
     7981 tatagattag gatagcctat gctacccaga tatagattag gatagcatat gctatccaga
     8041 tatttgggta gtatatgcta cccatggcaa cattagccca ccgtgctctc agcgacctcg
     8101 tgaatatgag gaccaacaac cctgtgcttg gcgctcaggc gcaagtgtgt gtaatttgtc
     8161 ctccagatcg cagcaatcgc gcccctatct tggcccgccc acctacttat gcaggtattc
     8221 cccggggtgc cattagtggt tttgtgggca agtggtttga ccgcagtggt tagcggggtt
     8281 acaatcagcc aagttattac acccttattt tacagtccaa aaccgcaggg cggcgtgtgg
     8341 gggctgacgc gtgcccccac tccacaattt caaaaaaaag agtggccact tgtctttgtt
     8401 tatgggcccc attggcgtgg agccccgttt aattttcggg ggtgttagag acaaccagtg
     8461 gagtccgctg ctgtcggcgt ccactctctt tccccttgtt acaaatagag tgtaacaaca
     8521 tggttcacct gtcttggtcc ctgcctggga cacatcttaa taaccccagt atcatattgc
     8581 actaggatta tgtgttgccc atagccataa attcgtgtga gatggacatc cagtctttac
     8641 ggcttgtccc caccccatgg atttctattg ttaaagatat tcagaatgtt tcattcctac
     8701 actagtattt attgcccaag gggtttgtga gggttatatt ggtgtcatag cacaatgcca
     8761 ccactgaacc ccccgtccaa attttattct gggggcgtca cctgaaacct tgttttcgag
     8821 cacctcacat acaccttact gttcacaact cagcagttat tctattagct aaacgaagga
     8881 gaatgaagaa gcaggcgaag attcaggaga gttcactgcc cgctccttga tcttcagcca
     8941 ctgcccttgt gactaaaatg gttcactacc ctcgtggaat cctgacccca tgtaaataaa
     9001 accgtgacag ctcatggggt gggagatatc gctgttcctt aggacccttt tactaaccct
     9061 aattcgatag catatgcttc ccgttgggta acatatgcta ttgaattagg gttagtctgg
     9121 atagtatata ctactacccg ggaagcatat gctacccgtt tagggttaac aagggggcct
     9181 tataaacact attgctaatg ccctcttgag ggtccgctta tcggtagcta cacaggcccc
     9241 tctgattgac gttggtgtag cctcccgtag tcttcctggg cccctgggag gtacatgtcc
     9301 cccagcattg gtgtaagagc ttcagccaag agttacacat aaaggcaatg ttgtgttgca
     9361 gtccacagac tgcaaagtct gctccaggat gaaagccact cagtgttggc aaatgtgcac
     9421 atccatttat aaggatgtca actacagtca gagaacccct ttgtgtttgg tccccccccg
     9481 tgtcacatgt ggaacagggc ccagttggca agttgtacca accaactgaa gggattacat
     9541 gcactgcccc gcgaagaagg ggcagagatg tcgtagtcag gtttagttcg tccggggcgg
     9601 ggatcgatcc tctagagtcg attaattaat cgtgaggctc cggtgcccgt cagtgggcag
     9661 agcgcacatc gcccacagtc cccgagaagt tggggggagg ggtcggcaat tgaaccggtg
     9721 cctagagaag gtggcgcggg gtaaactggg aaagtgatgt cgtgtactgg ctccgccttt
     9781 ttcccgaggg tgggggagaa ccgtatataa gtgcagtagt cgccgtgaac gttctttttc
     9841 gcaacgggtt tgccgccaga acacaggtaa gtgccgtgtg tggttcccgc gggcctggcc
     9901 tctttacggg ttatggccct tgcgtgcctt gaattacttc cacctggctg cagtacgtga
     9961 ttcttgatcc cgagcttcgg gttggaagtg ggtgggagag ttcgaggcct tgcgcttaag
    10021 gagccccttc gcctcgtgct tgagttgagg cctggcctgg gcgctggggc cgccgcgtgc
    10081 gaatctggtg gcaccttcgc gcctgtctcg ctgctttcga taagtctcta gccatttaaa
    10141 atttttgatg acctgctgcg acgctttttt tctggcaaga tagtcttgta aatgcgggcc
    10201 aagatctgca cactggtatt tcggtttttg gggccgcggg cggcgacggg gcccgtgcgt
    10261 cccagcgcac atgttcggcg aggcggggcc tgcgagcgcg gccaccgaga atcggacggg
    10321 ggtagtctca agctggccgg cctgctctgg tgcctggcct cgcgccgccg tgtatcgccc
    10381 cgccctgggc ggcaaggctg gcccggtcgg caccagttgc gtgagcggaa agatggccgc
    10441 ttcccggccc tgctgcaggg agctcaaaat ggaggacgcg gcgctcggga gagcgggcgg
    10501 gtgagtcacc cacacaaagg aaaagggcct ttccgtcctc agccgtcgct tcatgtgact
    10561 ccacggagta ccgggcgccg tccaggcacc tcgattagtt ctcgagcttt tggagtacgt
    10621 cgtctttagg ttggggggag gggttttatg cgatggagtt tccccacact gagtgggtgg
    10681 agactgaagt taggccagct tggcacttga tgtaattctc cttggaattt gccctttttg
    10741 agtttggatc ttggttcatt ctcaagcctc agacagtggt tcaaagtttt tttcttccat
    10801 ttcaggtgtc gtgaagctag cgccaccatg gcgggacacc tggcttcgga tttcgccttc
    10861 tcgccccctc caggtggtgg aggtgatggg ccaggggggc cggagccggg ctgggttgat
    10921 cctcggacct ggctaagctt ccaaggccct cctggagggc caggaatcgg gccgggggtt
    10981 gggccaggct ctgaggtgtg ggggattccc ccatgccccc cgccgtatga gttctgtggg
    11041 gggatggcgt actgtgggcc ccaggttgga gtggggctag tgccccaagg cggcttggag
    11101 acctctcagc ctgagggcga agcaggagtc ggggtggaga gcaactccga tggggcctcc
    11161 ccggagccct gcaccgtcac ccctggtgcc gtgaagctgg agaaggagaa gctggagcaa
    11221 aacccggagg agtcccagga catcaaagct ctgcagaaag aactcgagca atttgccaag
    11281 ctcctgaagc agaagaggat caccctggga tatacacagg ccgatgtggg gctcaccctg
    11341 ggggttctat ttgggaaggt attcagccaa acgaccatct gccgctttga ggctctgcag
    11401 cttagcttca agaacatgtg taagctgcgg cccttgctgc agaagtgggt ggaggaagct
    11461 gacaacaatg aaaatcttca ggagatatgc aaagcagaaa ccctcgtgca ggcccgaaag
    11521 agaaagcgaa ccagtatcga gaaccgagtg agaggcaacc tggagaattt gttcctgcag
    11581 tgcccgaaac ccacactgca gcagatcagc cacatcgccc agcagcttgg gctcgagaag
    11641 gatgtggtcc gagtgtggtt ctgtaaccgg cgccagaagg gcaagcgatc aagcagcgac
    11701 tatgcacaac gagaggattt tgaggctgct gggtctcctt tctcaggggg accagtgtcc
    11761 tttcctctgg ccccagggcc ccattttggt accccaggct atgggagccc tcacttcact
    11821 gcactgtact cctcggtccc tttccctgag ggggaagcct ttccccctgt ctccgtcacc
    11881 actctgggct ctcccatgca ttcaaacgga agtggcgtga aacagacttt gaattttgac
    11941 cttctcaagt tggcgggaga cgtggagtcc aacccagggc ccatggctgt cagcgacgcg
    12001 ctgctcccat ctttctccac gttcgcgtct ggcccggcgg gaagggagaa gacactgcgt
    12061 caagcaggtg ccccgaataa ccgctggcgg gaggagctct cccacatgaa gcgacttccc
    12121 ccagtgcttc ccggccgccc ctatgacctg gcggcggcga ccgtggccac agacctggag
    12181 agcggcggag ccggtgcggc ttgcggcggt agcaacctgg cgcccctacc tcggagagag
    12241 accgaggagt tcaacgatct cctggacctg gactttattc tctccaattc gctgacccat
    12301 cctccggagt cagtggccgc caccgtgtcc tcgtcagcgt cagcctcctc ttcgtcgtcg
    12361 ccgtcgagca gcggccctgc cagcgcgccc tccacctgca gcttcaccta tccgatccgg
    12421 gccgggaacg acccgggcgt ggcgccgggc ggcacgggcg gaggcctcct ctatggcagg
    12481 gagtccgctc cccctccgac ggctcccttc aacctggcgg acatcaacga cgtgagcccc
    12541 tcgggcggct tcgtggccga gctcctgcgg ccagaattgg acccggtgta cattccgccg
    12601 cagcagccgc agccgccagg tggcgggctg atgggcaagt tcgtgctgaa ggcgtcgctg
    12661 agcgcccctg gcagcgagta cggcagcccg tcggtcatca gcgtcagcaa aggcagccct
    12721 gacggcagcc acccggtggt ggtggcgccc tacaacggcg ggccgccgcg cacgtgcccc
    12781 aagatcaagc aggaggcggt ctcttcgtgc acccacttgg gcgctggacc ccctctcagc
    12841 aatggccacc ggccggctgc acacgacttc cccctggggc ggcagctccc cagcaggact
    12901 accccgaccc tgggtcttga ggaagtgctg agcagcaggg actgtcaccc tgccctgccg
    12961 cttcctcccg gcttccatcc ccacccgggg cccaattacc catccttcct gcccgatcag
    13021 atgcagccgc aagtcccgcc gctccattac caagagctca tgccacccgg ttcctgcatg
    13081 ccagaggagc ccaagccaaa gaggggaaga cgatcgtggc cccggaaaag gaccgccacc
    13141 cacacttgtg attacgcggg ctgcggcaaa acctacacaa agagttccca tctcaaggca
    13201 cacctgcgaa cccacacagg tgagaaacct taccactgtg actgggacgg ctgtggatgg
    13261 aaattcgccc gctcagatga actgaccagg cactaccgta aacacacggg gcaccgcccg
    13321 ttccagtgcc aaaaatgcga ccgagcattt tccaggtcgg accacctcgc cttacacatg
    13381 aagaggcatt ttggcagcgg cgagggcagg ggcagcctgc tgacctgcgg cgacgtggag
    13441 gagaaccccg gccccatgta caacatgatg gagacggagc tgaagccgcc gggcccgcag
    13501 caaacttcgg ggggcggcgg cggcaactcc accgcggcgg cggccggcgg caaccagaaa
    13561 aacagcccgg accgcgtcaa gcggcccatg aatgccttca tggtgtggtc ccgcgggcag
    13621 cggcgcaaga tggcccagga gaaccccaag atgcacaact cggagatcag caagcgcctg
    13681 ggcgccgagt ggaaactttt gtcggagacg gagaagcggc cgttcatcga cgaggctaag
    13741 cggctgcgag cgctgcacat gaaggagcac ccggattata aataccggcc ccggcggaaa
    13801 accaagacgc tcatgaagaa ggataagtac acgctgcccg gcgggctgct ggcccccggc
    13861 ggcaatagca tggcgagcgg ggtcggggtg ggcgccggcc tgggcgcggg cgtgaaccag
    13921 cgcatggaca gttacgcgca catgaacggc tggagcaacg gcagctacag catgatgcag
    13981 gaccagctgg gctacccgca gcacccgggc ctcaatgcgc acggcgcagc gcagatgcag
    14041 cccatgcacc gctacgacgt gagcgccctg cagtacaact ccatgaccag ctcgcagacc
    14101 tacatgaacg gctcgcccac ctacagcatg tcctactcgc agcagggcac ccctggcatg
    14161 gctcttggct ccatgggttc ggtggtcaag tccgaggcca gctccagccc ccctgtggtt
    14221 acctcttcct cccactccag ggcgccctgc caggccgggg acctccggga catgatcagc
    14281 atgtatctcc ccggcgccga ggtgccggaa cccgccgccc ccagcagact tcacatgtcc
    14341 cagcactacc agagcggccc ggtgcccggc acggccatta acggcacact gcccctctca
    14401 cacatgggta gtgggcaatg tactaactac gctttgttga aactcgctgg cgatgttgaa
    14461 agtaaccccg gtcctatgcc cctcaacgtt agcttcacca acaggaacta tgacctcgac
    14521 tacgactcgg tgcagccgta tttctactgc gacgaggagg agaacttcta ccagcagcag
    14581 cagcagagcg agctgcagcc cccggcgccc agcgaggata tctggaagaa attcgagctg
    14641 ctgcccaccc cgcccctgtc ccctagccgc cgctccgggc tctgctcgcc ctcctacgtt
    14701 gcggtcacac ccttctccct tcggggagac aacgacggcg gtggcgggag cttctccacg
    14761 gccgaccagc tggagatggt gaccgagctg ctgggaggag acatggtgaa ccagagtttc
    14821 atctgcgacc cggacgacga gaccttcatc aaaaacatca tcatccagga ctgtatgtgg
    14881 agcggcttct cggccgccgc caagctcgtc tcagagaagc tggcctccta ccaggctgcg
    14941 cgcaaagaca gcggcagccc gaaccccgcc cgcggccaca gcgtctgctc cacctccagc
    15001 ttgtacctgc aggatctgag cgccgccgcc tcagagtgca tcgacccctc ggtggtcttc
    15061 ccctaccctc tcaacgacag cagctcgccc aagtcctgcg cctcgcaaga ctccagcgcc
    15121 ttctctccgt cctcggattc tctgctctcc tcgacggagt cctccccgca gggcagcccc
    15181 gagcccctgg tgctccatga ggagacaccg cccaccacca gcagcgactc tgaggaggaa
    15241 caagaagatg aggaagaaat cgatgttgtt tctgtggaaa agaggcaggc tcctggcaaa
    15301 aggtcagagt ctggatcacc ttctgctgga ggccacagca aacctcctca cagcccactg
    15361 gtcctcaaga ggtgccacgt ctccacacat cagcacaact acgcagcgcc tccctccact
    15421 cggaaggact atcctgctgc caagagggtc aagttggaca gtgtcagagt cctgagacag
    15481 atcagcaaca accgaaaatg caccagcccc aggtcctcgg acaccgagga gaatgtcaag
    15541 aggcgaacac acaacgtctt ggagcgccag aggaggaacg agctaaaacg gagctttttt
    15601 gccctgcgtg accagatccc ggagttggaa aacaatgaaa aggcccccaa ggtagttatc
    15661 cttaaaaaag ccacagcata catcctgtcc gtccaagcag aggagcaaaa gctcatttct
    15721 gaagaggact tgttgcggaa acgacgagaa cagttgaaac acaaacttga acagctacgg
    15781 aactcttgtg cgtaatagcg gccgcgactc tagatcataa tcagccatac cacatttgta
    15841 gaggttttac ttgctttaaa aaacctccca cacctccccc tgaacctgaa acataaaatg
    15901 aatgcaattg ttgttgttaa cttgtttatt gcagcttata atggttacaa ataaagcaat
    15961 agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc
    16021 aaactcatca atgtatctta aggcgtaaat tgtaagcgtt aatattttgt taaaattcgc
    16081 gttaaatttt tgttaaatca gctcattttt taaccaatag gccgaaatcg gcaaaatccc
    16141 ttataaatca aaagaataga ccgagatagg gttgagtgtt gttccagttt ggaacaagag
    16201 tccactatta aagaacgtgg actccaacgt caaagggcga aaaaccgtct atcagggcga
    16261 tggcccacta cgtgaaccat caccctaatc aagttttttg gggtcgaggt gccgtaaagc
    16321 actaaatcgg aaccctaaag ggagcccccg atttagagct tgacggggaa agccggcgaa
    16381 cgtggcgaga aaggaaggga agaaagcgaa aggagcgggc gctagggcgc tggcaagtgt
    16441 agcggtcacg ctgcgcgtaa ccaccacacc cgccgcgctt aatgcgccgc tacagggcgc
    16501 gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat
    16561 acagcgctag ttattaatag taatcaatta cggggtcatt agttcatagc ccatatatgg
    16621 agttccgcgt tacataactt acggtaaatg gcccgcctgg ctgaccgccc aacgaccccc
    16681 gcccattgac gtcaataatg acgtatgttc ccatagtaac gccaataggg actttccatt
    16741 gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt ggcagtacat caagtgtatc
    16801 atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg
    16861 cccagtacat gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg
    16921 ctattaccat ggtcgaggtg agccccacgt tctgcttcac tctccccatc tcccccccct
    16981 ccccaccccc aattttgtat ttatttattt tttaattatt ttgtgcagcg atgggggcgg
    17041 gggggggggg ggggcgcgcg ccaggcgggg cggggcgggg cgaggggcgg ggcggggcga
    17101 ggcggagagg tgcggcggca gccaatcaga gcggcgcgct ccgaaagttt ccttttatgg
    17161 cgaggcggcg gcggcggcgg ccctataaaa agcgaagcgc gcggcgggcg ggagtcgctg
    17221 cgcgctgcct tcgccccgtg ccccgctccg ccgccgcctc gcgccgcccg ccccggctct
    17281 gactgaccgc gttactccca caggtgagcg ggcgggacgg cccttctcct ccgggctgta
    17341 attagcgctt ggtttaatga cggcttgttt cttttctgtg gctgcgtgaa agccttgagg
    17401 ggctccggga gggccctttg tgcgggggga gcggctcggg gggtgcgtgc gtgtgtgtgt
    17461 gcgtggggag cgccgcgtgc ggctccgcgc tgcccggcgg ctgtgagcgc tgcgggcgcg
    17521 gcgcggggct ttgtgcgctc cgcagtgtgc gcgaggggag cgcggccggg ggcggtgccc
    17581 cgcggtgcgg ggggggctgc gaggggaaca aaggctgcgt gcggggtgtg tgcgtggggg
    17641 ggtgagcagg gggtgtgggc gcgtcggtcg ggctgcaacc ccccctgcac ccccctcccc
    17701 gagttgctga gcacggcccg gcttcgggtg cggggctccg tacggggcgt ggcgcggggc
    17761 tcgccgtgcc gggcgggggg tggcggcagg tgggggtgcc gggcggggcg gggccgcctc
    17821 gggccgggga gggctcgggg gaggggcgcg gcggcccccg gagcgccggc ggctgtcgag
    17881 gcgcggcgag ccgcagccat tgccttttat ggtaatcgtg cgagagggcg cagggacttc
    17941 ctttgtccca aatctgtgcg gagccgaaat ctgggaggcg ccgccgcacc ccctctagcg
    18001 ggcgcggggc gaagcggtgc ggcgccggca ggaaggaaat gggcggggag ggccttcgtg
    18061 cgtcgccgcg ccgccgtccc cttctccctc tccagcctcg gggctgtccg cggggggacg
    18121 gctgccttcg ggggggacgg ggcagggcgg ggttcggctt ctggcgtgtg accggcggct
    18181 ctagagcctc tgctaaccat gttcatgcct tcttcttttt cctacagctc ctgggcaacg
    18241 tgctggttat tgtgctgtct catcattttg gcaaagaatt ctagggataa cagggtaata
    18301 tggcttcgta ccccggccat caacacgcgt ctgcgttcga ccaggctgcg cgttctcgcg
    18361 gccatagcaa ccgacgtacg gcgttgcgcc ctcgccggca gcaagaagcc acggaagtcc
    18421 gcccggagca gaaaatgccc acgctactgc gggtttatat agacggtccc cacgggatgg
    18481 ggaaaaccac caccacgcaa ctgctggtgg ccctgggttc gcgcgacgat atcgtctacg
    18541 tacccgagcc gatgacttac tggcgggtgc tgggggcttc cgagacaatc gcgaacatct
    18601 acaccacaca acaccgcctc gaccagggtg agatatcggc cggggacgcg gcggtggtaa
    18661 tgacaagcgc ccagataaca atgggcatgc cttatgccgt gaccgacgcc gttctggctc
    18721 ctcatatcgg gggggaggct gggagctcac atgccccgcc cccggccctc accctcatct
    18781 tcgaccgcca tcccatcgcc gccctcctgt gctacccggc cgcgcggtac cttatgggca
    18841 gcatgacccc ccaggccgtg ctggcgttcg tggccctcat cccgccgacc ttgcccggca
    18901 ccaacatcgt gcttggggcc cttccggagg acagacacat cgaccgcctg gccaaacgcc
    18961 agcgccccgg cgagcggctg gacctggcta tgctggctgc gattcgccgc gtttacgggc
    19021 tacttgccaa tacggtgcgg tatctgcagt gcggcgggtc gtggcgggag gactggggac
    19081 agctttcggg gacggccgtg ccgccccagg gtgccgagcc ccagagcaac gcgggcccac
    19141 gaccccatat cggggacacg ttatttaccc tgtttcgggc ccccgagttg ctggccccca
    19201 acggcgacct gtataacgtg tttgcctggg ccttggacgt cttggccaaa cgcctccgtt
    19261 ccatgcacgt ctttatcctg gattacgacc aatcgcccgc cggctgccgg gacgccctgc
    19321 tgcaacttac ctccgggatg gtccagaccc acgtcaccac ccccggctcc ataccgacga
    19381 tatgcgacct ggcgcgcacg tttgcccggg agatggggga ggctaactga gcggccgcgg
    19441 atccgcccct ctccctcccc cccccctaac gttactggcc gaagccgctt ggaataaggc
    19501 cggtgtgcgt ttgtctatat gttattttcc accatattgc cgtcttttgg caatgtgagg
    19561 gcccggaaac ctggccctgt cttcttgacg agcattccta ggggtctttc ccctctcgcc
    19621 aaaggaatgc aaggtctgtt gaatgtcgtg aaggaagcag ttcctctgga agcttcttga
    19681 agacaaacaa cgtctgtagc gaccctttgc aggcagcgga accccccacc tggcgacagg
    19741 tgcctctgcg gccaaaagcc acgtgtataa gatacacctg caaaggcggc acaaccccag
    19801 tgccacgttg tgagttggat agttgtggaa agagtcaaat ggctctcctc aagcgtattc
    19861 aacaaggggc tgaaggatgc ccagaaggta ccccattgta tgggatctga tctggggcct
    19921 cggtgcacat gctttacatg tgtttagtcg aggttaaaaa aacgtctagg ccccccgaac
    19981 cacggggacg tggttttcct ttgaaaaaca cgatgataat atggccacaa ccatgggatc
    20041 cgccattgaa caagatggat tgcacgcagg ttctccggcc gcttgggtgg agaggctatt
    20101 cggctatgac tgggcacaac agacaatcgg ctgctctgat gccgccgtgt tccggctgtc
    20161 agcgcagggg cgcccggttc tttttgtcaa gaccgacctg tccggtgccc tgaatgaact
    20221 gcaggacgag gcagcgcggc tatcgtggct ggccacgacg ggcgttcctt gcgcagctgt
    20281 gctcgacgtt gtcactgaag cgggaaggga ctggctgcta ttgggcgaag tgccggggca
    20341 ggatctcctg tcatctcacc ttgctcctgc cgagaaagta tccatcatgg ctgatgcaat
    20401 gcggcggctg catacgcttg atccggctac ctgcccattc gaccaccaag cgaaacatcg
    20461 catcgagcga gcacgtactc ggatggaagc cggtcttgtc gatcaggatg atctggacga
    20521 agagcatcag gggctcgcgc cagccgaact gttcgccagg ctcaaggcgc gcatgcccga
    20581 cggcgaggat ctcgtcgtga cccatggcga tgcctgcttg ccgaatatca tggtggaaaa
    20641 tggccgcttt tctggattca tcgactgtgg ccggctgggt gtggcggacc gctatcagga
    20701 catagcgttg gctacccgtg atattgctga agagcttggc ggcgaatggg ctgaccgctt
    20761 cctcgtgctt tacggtatcg ccgctcccga ttcgcagcgc atcgccttct atcgccttct
    20821 tgacgagttc ttctgagcgg gactctgggg ttcgaaatga ccgaccaagc gacgcccaac
    20881 ctgccatcac gagatttcga ttccaccgcc gccttctatg aaaggttggg cttcggaatc
    20941 gttttccggg acgccggctg gatgatcctc cagcgcgggg atctcatgct ggagttcttc
    21001 gcccacccta gggggaggct aactgaaaca cggaaggaga caataccgga aggaacccgc
    21061 gctatgacgg caataaaaag acagaataaa acgcacggtg ttgggtcgtt tgttcggcgc
    21121 ggcgattaat cgtgaggctc cggtgcccgt cagtgggcag agcgcacatc gcccacagtc
    21181 cccgagaagt tggggggagg ggtcggcaat tgaaccggtg cctagagaag gtggcgcggg
    21241 gtaaactggg aaagtgatgt cgtgtactgg ctccgccttt ttcccgaggg tgggggagaa
    21301 ccgtatataa gtgcagtagt cgccgtgaac gttctttttc gcaacgggtt tgccgccaga
    21361 acacaggtaa gtgccgtgtg tggttcccgc gggcctggcc tctttacggg ttatggccct
    21421 tgcgtgcctt gaattacttc cacctggctg cagtacgtga ttcttgatcc cgagcttcgg
    21481 gttggaagtg ggtgggagag ttcgaggcct tgcgcttaag gagccccttc gcctcgtgct
    21541 tgagttgagg cctggcctgg gcgctggggc cgccgcgtgc gaatctggtg gcaccttcgc
    21601 gcctgtctcg ctgctttcga taagtctcta gccatttaaa atttttgatg acctgctgcg
    21661 acgctttttt tctggcaaga tagtcttgta aatgcgggcc aagatctgca cactggtatt
    21721 tcggtttttg gggccgcggg cggcgacggg gcccgtgcgt cccagcgcac atgttcggcg
    21781 aggcggggcc tgcgagcgcg gccaccgaga atcggacggg ggtagtctca agctggccgg
    21841 cctgctctgg tgcctggcct cgcgccgccg tgtatcgccc cgccctgggc ggcaaggctg
    21901 gcccggtcgg caccagttgc gtgagcggaa agatggccgc ttcccggccc tgctgcaggg
    21961 agctcaaaat ggaggacgcg gcgctcggga gagcgggcgg gtgagtcacc cacacaaagg
    22021 aaaagggcct ttccgtcctc agccgtcgct tcatgtgact ccacggagta ccgggcgccg
    22081 tccaggcacc tcgattagtt ctcgagcttt tggagtacgt cgtctttagg ttggggggag
    22141 gggttttatg cgatggagtt tccccacact gagtgggtgg agactgaagt taggccagct
    22201 tggcacttga tgtaattctc cttggaattt gccctttttg agtttggatc ttggttcatt
    22261 ctcaagcctc agacagtggt tcaaagtttt tttcttccat ttcaggtgtc gtgaagctag
    22321 cgatcgccac catgtcttct aattcagata ctggggattt acaagagtct ttaaagcacg
    22381 gacttacacc tattgtgtct caatttaaaa tggtgaatta ctcctatgat gaagatctgg
    22441 aagagctttg tcccgtgtgt ggagataaag tgtctgggta ccattatggg ctcctcacct
    22501 gtgaaagctg caagggattt tttaagcgaa cagtccaaaa taataaaagg tacacatgta
    22561 tagaaaacca gaactgccaa attgacaaaa cacagagaaa gcgttgtcct tactgtcgtt
    22621 ttcaaaaatg tctaagtgtt ggaatgaagc tagaagctgt aagggccgac cgaatgcgtg
    22681 gaggaaggaa taagtttggg ccaatgtaca agagagacag ggccctgaag caacagaaaa
    22741 aagccctcat ccgagccaat ggacttaagc tagaagccat gtctcaggtg atccaagcta
    22801 tgccctctga cctgaccatt tcctctgcaa ttcaaaacat ccactctgcc tccaaaggcc
    22861 tacctctgaa ccatgctgcc ttgcctccta cagactatga cagaagtccc tttgtaacat
    22921 cccccattag catgacaatg ccccctcacg gcagcctgca aggttaccaa acatatggcc
    22981 actttcctag ccgggccatc aagtctgagt acccagaccc ctataccagc tcacccgagt
    23041 ccataatggg ctattcatat atggatagtt accagacgag ctctccagca agcatcccac
    23101 atctgatact ggaacttttg aagtgtgagc cagatgagcc tcaagtccag gctaaaatca
    23161 tggcctattt gcagcaagag caggctaacc gaagcaagca cgaaaagctg agcacctttg
    23221 ggcttatgtg caaaatggca gatcaaactc tcttctccat tgtcgagtgg gccaggagta
    23281 gtatcttctt cagagaactt aaggttgatg accaaatgaa gctgcttcag aactgctgga
    23341 gtgagctctt aatcctcgac cacatttacc gacaagtggt acatggaaag gaaggatcca
    23401 tcttcctggt tactgggcaa caagtggact attccataat agcatcacaa gccggagcca
    23461 ccctcaacaa cctcatgagt catgcacagg agttagtggc aaaacttcgt tctctccagt
    23521 ttgatcaacg agagttcgta tgtctgaaat tcttggtgct ctttagttta gatgtcaaaa
    23581 accttgaaaa cttccagctg gtagaaggtg tccaggaaca agtcaatgcc gccctgctgg
    23641 actacacaat gtgtaactac ccgcagcaga cagagaaatt tggacagcta cttcttcgac
    23701 tacccgaaat ccgggccatc agtatgcagg ctgaagaata cctctactac aagcacctga
    23761 acggggatgt gccctataat aaccttctca ttgaaatgtt gcatgccaaa agagcataaa
    23821 tttaaatgtc gacgcggccg cgggactctg gggttcgaaa tgaccgacca agcgacgccc
    23881 aacctgccat cacgagattt cgattccacc gccgccttct atgaaaggtt gggcttcgga
    23941 atcgttttcc gggacgccgg ctggatgatc ctccagcgcg gggatctcat gctggagttc
    24001 ttcgcccacc ctagggggag gctaactgaa acacggaagg agacaatacc ggaaggaacc
    24061 cgcgctatga cggcaataaa aagacagaat aaaacgcacg gtgttgggtc gtttgttcgg
    24121 cgcgccctcg acattgatta ttgactagtt at
//