| 质粒名称: | pMO719 |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 117479 |
| 存储实验室: | Judy Wall |
|
载体骨架基本信息 |
|
| 载体名称: | pCR8/GW/TOPO |
| 载体出品公司: | Invitrogen |
| 载体抗性: | 大观霉素 |
| 空载体大小: | 2817 bp |
| 完整质粒大小: | -- |
| 载体修饰: | 捕获的pBG1片段 |
| 载体类型: | 细菌表达 |
| 筛选标记: | -- |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | -- |
| 物种来源: | -- |
| 插入大小: | -- |
| 基因突变: | -- |
| GenBank编号 | -- |
| 启动子 | -- |
| 标签/融合蛋白: | -- |
| 克隆方法: | TOPO克隆 |
| 5' 克隆位点: | -- |
| 3' 克隆位点: | -- |
| 5' 测序引物 | -- |
| 3' 测序引物 | -- |
|
文献引用方法 |
|
| 材料和方法部分: | pMO719 was a gift from Judy Wall (Addgene plasmid # 117479 ; http://n2t.net/addgene:117479 ; RRID:Addgene_117479) |
| 参考文献部分: | Development of a markerless genetic exchange system for Desulfovibrio vulgaris Hildenborough and its use in generating a strain with increased transformation efficiency. Keller KL, Bender KS, Wall JD. Appl Environ Microbiol. 2009 Dec;75(24):7682-91. doi: 10.1128/AEM.01839-09. Epub 2009 Oct 16. 10.1128/AEM.01839-09 PubMed 19837844 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pMO719 5111 bp ds-DNA circular SYN 12-MAY-2021
DEFINITION Desulfovibrio shuttle vector, contains pBG1 and pMB1 replicons,
specR.
ACCESSION .
VERSION .
KEYWORDS pMO719
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 5111)
AUTHORS Keller KL, Bender KS, Wall JD
TITLE Development of a markerless genetic exchange system for
Desulfovibrio vulgaris Hildenborough and its use in generating a
strain with increased transformation efficiency.
JOURNAL Appl Environ Microbiol. 2009 Dec;75(24):7682-91. doi:
10.1128/AEM.01839-09. Epub 2009 Oct 16.
PUBMED 19837844
REFERENCE 2 (bases 1 to 5111)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..5111
/organism="synthetic DNA construct"
/mol_type="other DNA"
primer_bind 92..109
/label=L4440
/note="L4440 vector, forward primer"
terminator 268..295
/label=rrnB T2 terminator
/note="transcription terminator T2 from the E. coli rrnB
gene"
terminator 387..473
/gene="Escherichia coli rrnB"
/label=rrnB T1 terminator
/note="transcription terminator T1 from the E. coli rrnB
gene"
primer_bind 522..544
/label=M13/pUC Forward
/note="In lacZ gene"
primer_bind 536..553
/label=M13 Forward
/note="In lacZ gene. Also called M13-F20 or M13 (-21)
Forward"
primer_bind 537..553
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
protein_bind 569..668
/gene="mutant version of attL"
/label=attL1
/bound_moiety="LR Clonase(TM)"
/note="recombination site for the Gateway(R) LR reaction"
protein_bind complement(2990..3089)
/gene="mutant version of attL"
/label=attL2
/bound_moiety="LR Clonase(TM)"
/note="recombination site for the Gateway(R) LR reaction"
primer_bind complement(3106..3125)
/label=T7
/note="T7 promoter, forward primer"
promoter complement(3107..3125)
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
primer_bind complement(3130..3146)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(3130..3146)
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
CDS 3577..4368
/codon_start=1
/gene="aadA"
/product="aminoglycoside adenylyltransferase (Murphy,
1985)"
/label=SmR
/note="confers resistance to spectinomycin and
streptomycin"
/translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
rep_origin 4461..5049
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 4950..4969
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
ORIGIN
1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac
601 ctgttcgttg caacaaattg atgagcaatg cttttttata atgccaactt tgtacaaaaa
661 agcaggctcc gaattcccct cggagccagc atcgtaatga ccgaaggccg aagcaagcga
721 agaggtcaag ggcagaagcg aagcgtccct tgaccggatg agcggcgtag gcagaaggga
781 atggagagga tgactacgaa ggggaattaa ttccccttgg tggagttgag tggttttaaa
841 aactggagag gagtccatga aacgaaagca gctgaggatt gaaacgaagt aacagcgcat
901 actaattgta ttttttgggt gggggaggag ccttgcgacg ggcggcgagc gtagcgaggg
961 gcctctatag cgcgcgcata cgcgcgcgcg cgcgcatacg cgcgcgcttc ttcttttctt
1021 tcttttctct tttccccctc gtttttcgtc agttatttcg gcaacctaca gacctaaaag
1081 tgaccaaaag tacgtctaaa ggtgaccaaa agtacgtcta aaggtgacca aaagtacgtc
1141 taaaggtgac caaaagtacg tggtgtagcc tttgggtgac caatttgaca agtggtcacc
1201 tttagccata aaggcaaaag aacagcccga aaaggtgcgg taacacctca acgggcctaa
1261 cctctcacaa gccctaaaat ggagggtctg caatggttga caggcaatta accttacccg
1321 gcgagatagt caaaaagtca aacgcactgg ctagggccaa gtggtcccct aagtccgtgt
1381 gggaaccgcg catggtcgcg ctggtagctt ccatggtccg tcaggacgat gcggactttc
1441 aggaataccg ctttcctgtc tccacccttt gcgatgagca agagctatcc ggcaagaggt
1501 acaaggacat taaggccgct gtagaaaatg tgatgaaagc ctatgtgatg attgaggaag
1561 gcaaaaatta cgtggcgtat agcatcttct caaagtgtgg ctatgaaaac ggtgtcatgg
1621 tcgccgcctt ccatcccgat ctgaaacccc attttctcgg ccttcaaaag cagttcacaa
1681 ggtatagcct catggaattt ctggtgctgc ctagcaccta cagccaaagg gttttcgagg
1741 ttttgaggtc gtgggacgac aagccggaag tgaccatttc acttgtcgat cttcatgaaa
1801 tggtgggtac gcctccttct ctgcggaaga agtaccccga ctttcgccgt aaggttcttg
1861 agaaggccca caaggacata aaccgccata ccgcgcttgc ttacgagtgg gaagccgtga
1921 agaacgggcg ctcggtcgcg ggtatccgct tcctgttcgc gcagaagcgg gcgggcaggg
1981 tcgtagccaa gaaacaggcc aaggtcgtgc aggacagcag cgacgccaag aacaaggcct
2041 tcatcgccgc gaccaagtgc cacgcggcca gcaagggccg ctgcacgccg aaagacaacc
2101 tccagtgcca agtgtgcgcc cagctcgtca gcctgggcaa gagagccgga acagcctaaa
2161 cctcttttgt agcacccccc ctttgaggct gtagcacccc ccttttgagg ctgtagcacc
2221 ttgtgacgtg cctgtagcac cttttcagac ttcacatttt taaccataac gtgctgtttt
2281 aacagaaatt ttctttacgt ttcagcaccg gctgatctcg cttttttgtg ccacaaaaag
2341 gctatcctgc caagcggcat taaaacaagc cacctcatca acaaaatgaa ggggggcgcg
2401 gcccgctcac atggttgcag gtgaagaaga ttggaaatcg aggcgaaggt tattccattc
2461 cccgcagtag aacctgaaca gggaagcctg ttctaatagc cctaaaatcg attttaagcg
2521 caggaacaag caagaagaga ctcaggctca ccgagggaaa tttcctccct tacgccccct
2581 tctacgcttc gttatcgcta cgccgaaaaa aagagcaagg gacgcttcgc ttctgccctt
2641 gctctttttt tgcgccttcg ctgcactccg ctcacgaagg cggctccggg ggaaatttca
2701 gcctggttcc aacgacaagc gaagcgggat ctgcgggggg cccccgtgag ggggggcggc
2761 tagccccatc ccctgcttgc aggggcagga ccggcgtagc cggagcgagt ggtgatggtc
2821 ttgtgatcgt ccagagtgat ttccgttgtg ctggactctc ccgattctcg acgagctgaa
2881 agcgagaaga gcgcacttat acatcgctgc gcgacgtgtg cgccctgcgg ggctaagtca
2941 tcggaacaaa agagtttccc cgtagtgggg aaaagggggc ttgaattcga cccagctttc
3001 ttgtacaaag ttggcattat aaaaaataat tgctcatcaa tttgttgcaa cgaacaggtc
3061 actatcagtc aaaataaaat cattatttgc catccagctg atatccccta tagtgagtcg
3121 tattacatgg tcatagctgt ttcctggcag ctctggcccg tgtctcaaaa tctctgatgt
3181 tacattgcac aagataaaaa tatatcatca tgcctcctct agaccagcca ggacagaaat
3241 gcctcgactt cgctgctacc caaggttgcc gggtgacgca caccgtggaa acggatgaag
3301 gcacgaaccc agtggacata agcctgttcg gttcgtaagc tgtaatgcaa gtagcgtatg
3361 cgctcacgca actggtccag aaccttgacc gaacgcagcg gtggtaacgg cgcagtggcg
3421 gttttcatgg cttgttatga ctgttttttt ggggtacagt ctatgcctcg ggcatccaag
3481 cagcaagcgc gttacgccgt gggtcgatgt ttgatgttat ggagcagcaa cgatgttacg
3541 cagcagggca gtcgccctaa aacaaagtta aacattatga gggaagcggt gatcgccgaa
3601 gtatcgactc aactatcaga ggtagttggc gtcatcgagc gccatctcga accgacgttg
3661 ctggccgtac atttgtacgg ctccgcagtg gatggcggcc tgaagccaca cagtgatatt
3721 gatttgctgg ttacggtgac cgtaaggctt gatgaaacaa cgcggcgagc tttgatcaac
3781 gaccttttgg aaacttcggc ttcccctgga gagagcgaga ttctccgcgc tgtagaagtc
3841 accattgttg tgcacgacga catcattccg tggcgttatc cagctaagcg cgaactgcaa
3901 tttggagaat ggcagcgcaa tgacattctt gcaggtatct tcgagccagc cacgatcgac
3961 attgatctgg ctatcttgct gacaaaagca agagaacata gcgttgcctt ggtaggtcca
4021 gcggcggagg aactctttga tccggttcct gaacaggatc tatttgaggc gctaaatgaa
4081 accttaacgc tatggaactc gccgcccgac tgggctggcg atgagcgaaa tgtagtgctt
4141 acgttgtccc gcatttggta cagcgcagta accggcaaaa tcgcgccgaa ggatgtcgct
4201 gccgactggg caatggagcg cctgccggcc cagtatcagc ccgtcatact tgaagctaga
4261 caggcttatc ttggacaaga agaagatcgc ttggcctcgc gcgcagatca gttggaagaa
4321 tttgtccact acgtgaaagg cgagatcacc aaggtagtcg gcaaataacc ctcgagccac
4381 ccatgaccaa aatcccttaa cgtgagttac gcgtcgttcc actgagcgtc agaccccgta
4441 gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa
4501 acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt
4561 tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag
4621 ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta
4681 atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca
4741 agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag
4801 cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa
4861 agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga
4921 acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc
4981 gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc
5041 ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt
5101 gctcacatgt t
//
