| 质粒名称: | Lenti-LucOS |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 22777 |
| 存储实验室: | DEPOSITING LAB |
|
载体骨架基本信息 |
|
| 载体名称: | Luc.Cre (aka: UbC.Luciferase.PGK.Cre) |
| 载体出品公司: | Available at Addgene (#20905) |
| 载体类型: | 哺乳细胞表达，慢病毒，Cre/Lox |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | Stbl3 |
| 拷贝数: | 高拷贝 |
|
插入基因信息 |
|
| 插入基因名称: | OVA-SIY |
| 标签/融合蛋白: | luciferase (N terminal on backbone) |
| 克隆方法: | 限制性内切酶 |
| 3’测序引物: | MSCV-REV |
|
文献引用方法 |
|
| 材料和方法部分: | Lenti-LucOS was a gift from Tyler Jacks (Addgene plasmid # 22777 ; http://n2t.net/addgene:22777 ; RRID:Addgene_22777) |
| 参考文献部分: |
Lymphatic-preserving treatment sequencing with immune checkpoint inhibition unleashes cDC1-dependent antitumor immunity in HNSCC. Saddawi-Konefka R, O'Farrell A, Faraji F, Clubb L, Allevato MM, Jensen SM, Yung BS, Wang Z, Wu VH, Anang NA, Msari RA, Schokrpur S, Pietryga IF, Molinolo AA, Mesirov JP, Simon AB, Fox BA, Bui JD, Sharabi A, Cohen EEW, Califano JA, Gutkind JS. Nat Commun. 2022 Jul 25;13(1):4298. doi: 10.1038/s41467-022-31941-w. PubMed
NAFLD indirectly impairs antigen-specific CD8(+) T cell immunity against liver cancer in mice. McVey JC, Green BL, Ruf B, McCallen JD, Wabitsch S, Subramanyam V, Diggs LP, Heinrich B, Greten TF, Ma C. iScience. 2022 Feb 1;25(2):103847. doi: 10.1016/j.isci.2022.103847. eCollection 2022 Feb 18. PubMed
Targeting the Atf7ip-Setdb1 Complex Augments Antitumor Immunity by Boosting Tumor Immunogenicity. Hu H, Khodadadi-Jamayran A, Dolgalev I, Cho H, Badri S, Chiriboga LA, Zeck B, Lopez De Rodas Gregorio M, Dowling CM, Labbe K, Deng J, Chen T, Zhang H, Zappile P, Chen Z, Ueberheide B, Karatza A, Han H, Ranieri M, Tang S, Jour G, Osman I, Sucker A, Schadendorf D, Tsirigos A, Schalper KA, Velcheti V, Huang HY, Jin Y, Ji H, Poirier JT, Li F, Wong KK. Cancer Immunol Res. 2021 Nov;9(11):1298-1315. doi: 10.1158/2326-6066.CIR-21-0543. Epub 2021 Aug 30. PubMed
Protocol to establish a lung adenocarcinoma immunotherapy allograft mouse model with FACS and immunofluorescence-based analysis of tumor response. Peng Z, Yu M, Lin J, Dong T, Zhang X, Shi M, Qin M, Li S, Guo W, Zhang H, Sun S. STAR Protoc. 2021 Jun 11;2(2):100595. doi: 10.1016/j.xpro.2021.100595. eCollection 2021 Jun 18. PubMed
IL-33 Signaling Alters Regulatory T Cell Diversity in Support of Tumor Development. Li A, Herbst RH, Canner D, Schenkel JM, Smith OC, Kim JY, Hillman M, Bhutkar A, Cuoco MS, Rappazzo CG, Rogers P, Dang C, Jerby-Arnon L, Rozenblatt-Rosen O, Cong L, Birnbaum M, Regev A, Jacks T. Cell Rep. 2019 Dec 3;29(10):2998-3008.e8. doi: 10.1016/j.celrep.2019.10.120. PubMed
beta-catenin activation promotes immune escape and resistance to anti-PD-1 therapy in hepatocellular carcinoma. Ruiz de Galarreta M, Bresnahan E, Molina-Sanchez P, Lindblad KE, Maier B, Sia D, Puigvehi M, Miguela V, Casanova-Acebes M, Dhainaut M, Villacorta-Martin C, Singhi AD, Moghe A, von Felden J, Tal Grinspan L, Wang S, Kamphorst AO, Monga SP, Brown BD, Villanueva A, Llovet JM, Merad M, Lujambio A. Cancer Discov. 2019 Jun 11. pii: 2159-8290.CD-19-0074. doi: 10.1158/2159-8290.CD-19-0074. PubMed
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买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS Lenti-LucOS 10739 bp ds-DNA circular SYN 12-MAY-2021
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS Lenti-LucOS
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 10739)
TITLE Jacks Lab Unpublished Plasmids
REFERENCE 2 (bases 1 to 10739)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..10739
/organism="synthetic DNA construct"
/mol_type="other DNA"
primer_bind complement(47..66)
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
enhancer 238..617
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 619..817
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind 772..792
/label=CMV-F
/note="Human CMV immediate early promoter, forward primer"
LTR 835..1015
/label=5' LTR (truncated)
/note="truncated 5' long terminal repeat (LTR) from HIV-1"
misc_feature 1062..1187
/label=HIV-1 Psi
/note="packaging signal of human immunodeficiency virus
type 1"
misc_feature 1686..1919
/label=RRE
/note="The Rev response element (RRE) of HIV-1 allows for
Rev-dependent mRNA export from the nucleus to the
cytoplasm."
CDS 2104..2148
/codon_start=1
/product="antigenic peptide corresponding to amino acids
655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
et al., 2013)"
/label=gp41 peptide
/note="recognized by the 2H10 single-chain llama nanobody"
/translation="KNEQELLELDKWASL"
misc_feature 2446..2563
/label=cPPT/CTS
/note="central polypurine tract and central termination
sequence of HIV-1"
promoter 2632..3841
/label=UbC promoter
/note="human ubiquitin C promoter"
primer_bind 3678..3697
/label=hUBCpro-F
/note="Human Ubiquitin C (UbC) promoter, forward primer"
CDS 3868..5517
/codon_start=1
/gene="luc+"
/product="firefly luciferase"
/label=luciferase
/note="enhanced luc+ version of the luciferase gene"
/translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
primer_bind complement(3934..3952)
/label=LucNrev
/note="Firefly luciferase, reverse primer"
CDS 5530..5553
/codon_start=1
/product="antigenic CD8+ T cell epitope from chicken
ovalbumin"
/label=OVA peptide
/translation="SIINFEKL"
CDS 5971..6000
/codon_start=1
/product="Myc (human c-Myc proto-oncogene) epitope tag"
/label=Myc
/translation="EQKLISEEDL"
primer_bind complement(6055..6078)
/label=MSCV-rev
/note="Murine stem cell virus, reverse primer"
primer_bind 6452..6471
/label=mPGK-F
/note="Mouse PGK promoter, forward primer"
CDS 6523..6543
/codon_start=1
/product="nuclear localization signal of SV40 (simian virus
40) large T antigen"
/label=SV40 NLS
/translation="PKKKRKV"
CDS 6544..7572
/codon_start=1
/product="site-specific recombinase"
/label=Cre
/note="Cre is a site-specific recombinase from
bacteriophage P1. Recombination occurs at loxP sequences."
/translation="SNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKMLL
SVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGLP
RPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAFL
GIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVER
WISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQR
YLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLED
GD"
misc_feature 7628..8216
/label=WPRE
/note="woodchuck hepatitis virus posttranscriptional
regulatory element"
primer_bind complement(7681..7701)
/label=WPRE-R
/note="WPRE, reverse primer"
CDS complement(8099..8110)
/codon_start=1
/product="Factor Xa recognition and cleavage site"
/label=Factor Xa site
/translation="IEGR"
primer_bind complement(8219..8235)
/label=KS primer
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(8220..8236)
/label=pBluescriptKS
/note="For pBluescript vector"
LTR 8745..8925
/label=5' LTR (truncated)
/note="truncated 5' long terminal repeat (LTR) from HIV-1"
rep_origin complement(8987..9575)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(9067..9086)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(9746..10606)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 10369..10388
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(10607..10711)
/gene="bla"
/label=AmpR promoter
ORIGIN
1 gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg
61 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt
121 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc
181 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac
241 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat
301 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
361 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
421 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
481 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
541 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
601 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt
661 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
721 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg
781 gcggtaggcg tgtacggtgg gaggtctata taagcagcgc gttttgcctg tactgggtct
841 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
901 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
961 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
1021 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
1081 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
1141 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
1201 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
1261 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
1321 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
1381 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
1441 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
1501 aaagtaagac caccgcacag caagcggccg gccgcgctga tcttcagacc tggaggagga
1561 gatatgaggg acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca
1621 ttaggagtag cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg
1681 ggaataggag ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcg
1741 tcaatgacgc tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac
1801 aatttgctga gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc
1861 aagcagctcc aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg
1921 gggatttggg gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt
1981 tggagtaata aatctctgga acagatttgg aatcacacga cctggatgga gtgggacaga
2041 gaaattaaca attacacaag cttaatacac tccttaattg aagaatcgca aaaccagcaa
2101 gaaaagaatg aacaagaatt attggaatta gataaatggg caagtttgtg gaattggttt
2161 aacataacaa attggctgtg gtatataaaa ttattcataa tgatagtagg aggcttggta
2221 ggtttaagaa tagtttttgc tgtactttct atagtgaata gagttaggca gggatattca
2281 ccattatcgt ttcagaccca cctcccaacc ccgaggggac ccgacaggcc cgaaggaata
2341 gaagaagaag gtggagagag agacagagac agatccattc gattagtgaa cggatcggca
2401 ctgcgtgcgc caattctgca gacaaatggc agtattcatc cacaatttta aaagaaaagg
2461 ggggattggg gggtacagtg caggggaaag aatagtagac ataatagcaa cagacataca
2521 aactaaagaa ttacaaaaac aaattacaaa aattcaaaat tttcgggttt attacaggga
2581 cagcagagat ccagtttggt tagtaccggg cccgctctag acggttgatc tggcctccgc
2641 gccgggtttt ggcgcctccc gcgggcgccc ccctcctcac ggcgagcgct gccacgtcag
2701 acgaagggcg caggagcgtc ctgatccttc cgcccggacg ctcaggacag cggcccgctg
2761 ctcataagac tcggccttag aaccccagta tcagcagaag gacattttag gacgggactt
2821 gggtgactct agggcactgg ttttctttcc agagagcgga acaggcgagg aaaagtagtc
2881 ccttctcggc gattctgcgg agggatctcc gtggggcggt gaacgccgat gattatataa
2941 ggacgcgccg ggtgtggcac agctagttcc gtcgcagccg ggatttgggt cgcggttctt
3001 gtttgtggat cgctgtgatc gtcacttggt gagtagcggg ctgctgggct ggccggggct
3061 ttcgtggccg ccgggccgct cggtgggacg gaagcgtgtg gagagaccgc caagggctgt
3121 agtctgggtc cgcgagcaag gttgccctga actgggggtt ggggggagcg cagcaaaatg
3181 gcggctgttc ccgagtcttg aatggaagac gcttgtgagg cgggctgtga ggtcgttgaa
3241 acaaggtggg gggcatggtg ggcggcaaga acccaaggtc ttgaggcctt cgctaatgcg
3301 ggaaagctct tattcgggtg agatgggctg gggcaccatc tggggaccct gacgtgaagt
3361 ttgtcactga ctggagaact cggtttgtcg tctgttgcgg gggcggcagt tatgcggtgc
3421 cgttgggcag tgcacccgta cctttgggag cgcgcgccct cgtcgtgtcg tgacgtcacc
3481 cgttctgttg gcttataatg cagggtgggg ccacctgccg gtaggtgtgc ggtaggcttt
3541 tctccgtcgc aggacgcagg gttcgggcct agggtaggct ctcctgaatc gacaggcgcc
3601 ggacctctgg tgaggggagg gataagtgag gcgtcagttt ctttggtcgg ttttatgtac
3661 ctatcttctt aagtagctga agctccggtt ttgaactatg cgctcggggt tggcgagtgt
3721 gttttgtgaa gttttttagg caccttttga aatgtaatca tttgggtcaa tatgtaattt
3781 tcagtgttag actagtaaat tgtccgctaa attctggccg tttttggctt ttttgttaga
3841 cgaagctaac gcgctagcaa agaattcatg gaagacgcca aaaacataaa gaaaggcccg
3901 gcgccattct atccgctgga agatggaacc gctggagagc aactgcataa ggctatgaag
3961 agatacgccc tggttcctgg aacaattgct tttacagatg cacatatcga ggtggacatc
4021 acttacgctg agtacttcga aatgtccgtt cggttggcag aagctatgaa acgatatggg
4081 ctgaatacaa atcacagaat cgtcgtatgc agtgaaaact ctcttcaatt ctttatgccg
4141 gtgttgggcg cgttatttat cggagttgca gttgcgcccg cgaacgacat ttataatgaa
4201 cgtgaattgc tcaacagtat gggcatttcg cagcctaccg tggtgttcgt ttccaaaaag
4261 gggttgcaaa aaattttgaa cgtgcaaaaa aagctcccaa tcatccaaaa aattattatc
4321 atggattcta aaacggatta ccagggattt cagtcgatgt acacgttcgt cacatctcat
4381 ctacctcccg gttttaatga atacgatttt gtgccagagt ccttcgatag ggacaagaca
4441 attgcactga tcatgaactc ctctggatct actggtctgc ctaaaggtgt cgctctgcct
4501 catagaactg cctgcgtgag attctcgcat gccagagatc ctatttttgg caatcaaatc
4561 attccggata ctgcgatttt aagtgttgtt ccattccatc acggttttgg aatgtttact
4621 acactcggat atttgatatg tggatttcga gtcgtcttaa tgtatagatt tgaagaagag
4681 ctgtttctga ggagccttca ggattacaag attcaaagtg cgctgctggt gccaacccta
4741 ttctccttct tcgccaaaag cactctgatt gacaaatacg atttatctaa tttacacgaa
4801 attgcttctg gtggcgctcc cctctctaag gaagtcgggg aagcggttgc caagaggttc
4861 catctgccag gtatcaggca aggatatggg ctcactgaga ctacatcagc tattctgatt
4921 acacccgagg gggatgataa accgggcgcg gtcggtaaag ttgttccatt ttttgaagcg
4981 aaggttgtgg atctggatac cgggaaaacg ctgggcgtta atcaaagagg cgaactgtgt
5041 gtgagaggtc ctatgattat gtccggttat gtaaacaatc cggaagcgac caacgccttg
5101 attgacaagg atggatggct acattctgga gacatagctt actgggacga agacgaacac
5161 ttcttcatcg ttgaccgcct gaagtctctg attaagtaca aaggctatca ggtggctccc
5221 gctgaattgg aatccatctt gctccaacac cccaacatct tcgacgcagg tgtcgcaggt
5281 cttcccgacg atgacgccgg tgaacttccc gccgccgttg ttgttttgga gcacggaaag
5341 acgatgacgg aaaaagagat cgtggattac gtcgccagtc aagtaacaac cgcgaaaaag
5401 ttgcgcggag gagttgtgtt tgtggacgaa gtaccgaaag gtcttaccgg aaaactcgac
5461 gcaagaaaaa tcagagagat cctcataaag gccaagaagg gcggaaagat cgccgtggag
5521 cagctcgaga gtataatcaa ctttgaaaaa ctgactgaat ggaccagttc taatgttatg
5581 gaagagagga agatcaaagt gtacttacct cgcatgaaga tggaggaaaa atacaacctc
5641 acatctgtct taatggctat gggcattact gacgtgttta gctcttcagc caatctgtct
5701 ggcatctcct cagcagagag cctgaagata tctcaagctg tccatgcagc acatgcagaa
5761 atcaatgaag caggcagaga ggtggtaggg tcagcagagg ctggagtgga tgctgcaagc
5821 gtctctgaag aatttagggc tgaccatcca ttcctcttct gtatcaagca catcgcaacc
5881 aacgccgttc tcttctttgg cagatgtgtt ggatccgagc agctggagtc catctacagg
5941 tactacggcc tgctgctgaa ggaaagatcc gaacaaaagc ttatttctga agaggacttg
6001 taagcggccg cattctaccg ggtaggggag gcgcttttcc caaggcagtc tggagcatgc
6061 gctttagcag ccccgctggg cacttggcgc tacacaagtg gcctctggcc tcgcacacat
6121 tccacatcca ccggtaggcg ccaaccggct ccgttctttg gtggcccctt cgcgccacct
6181 tctactcctc ccctagtcag gaagttcccc cccgccccgc agctcgcgtc gtgcaggacg
6241 tgacaaatgg aagtagcacg tctcactagt ctcgtgcaga tggacagcac cgctgagcaa
6301 tggaagcggg taggcctttg gggcagcggc caatagcagc tttgctcctt cgctttctgg
6361 gctcagaggc tgggaagggg tgggtccggg ggcgggctca ggggcgggct caggggcggg
6421 gcgggcgccc gaaggtcctc cggaggcccg gcattctgca cgcttcaaaa gcgcacgtct
6481 gccgcgctgt tctcctcttc ctcatctccg ggacccggga tgcccaagaa gaagaggaag
6541 gtgtccaatt tactgaccgt acaccaaaat ttgcctgcat taccggtcga tgcaacgagt
6601 gatgaggttc gcaagaacct gatggacatg ttcagggatc gccaggcgtt ttctgagcat
6661 acctggaaaa tgcttctgtc cgtttgccgg tcgtgggcgg catggtgcaa gttgaataac
6721 cggaaatggt ttcccgcaga acctgaagat gttcgcgatt atcttctata tcttcaggcg
6781 cgcggtctgg cagtaaaaac tatccagcaa catttgggcc agctaaacat gcttcatcgt
6841 cggtccgggc tgccacgacc aagtgacagc aatgctgttt cactggttat gcggcggatc
6901 cgaaaagaaa acgttgatgc cggtgaacgt gcaaaacagg ctctagcgtt cgaacgcact
6961 gatttcgacc aggttcgttc actcatggaa aatagcgatc gctgccagga tatacgtaat
7021 ctggcatttc tggggattgc ttataacacc ctgttacgta tagccgaaat tgccaggatc
7081 agggttaaag atatctcacg tactgacggt gggagaatgt taatccatat tggcagaacg
7141 aaaacgctgg ttagcaccgc aggtgtagag aaggcactta gcctgggggt aactaaactg
7201 gtcgagcgat ggatttccgt ctctggtgta gctgatgatc cgaataacta cctgttttgc
7261 cgggtcagaa aaaatggtgt tgccgcgcca tctgccacca gccagctatc aactcgcgcc
7321 ctggaaggga tttttgaagc aactcatcga ttgatttacg gcgctaagga tgactctggt
7381 cagagatacc tggcctggtc tggacacagt gcccgtgtcg gagccgcgcg agatatggcc
7441 cgcgctggag tttcaatacc ggagatcatg caagctggtg gctggaccaa tgtaaatatt
7501 gtcatgaact atatccgtaa cctggatagt gaaacagggg caatggtgcg cctgctggaa
7561 gatggcgatt agccatgttt aagggttccg gttccactag gtacaattcg atatcaagct
7621 tatcgataat caacctctgg attacaaaat ttgtgaaaga ttgactggta ttcttaacta
7681 tgttgctcct tttacgctat gtggatacgc tgctttaatg cctttgtatc atgctattgc
7741 ttcccgtatg gctttcattt tctcctcctt gtataaatcc tggttgctgt ctctttatga
7801 ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac
7861 ccccactggt tggggcattg ccaccacctg tcagctcctt tccgggactt tcgctttccc
7921 cctccctatt gccacggcgg aactcatcgc cgcctgcctt gcccgctgct ggacaggggc
7981 tcggctgttg ggcactgaca attccgtggt gttgtcgggg aaatcatcgt cctttccttg
8041 gctgctcgcc tgtgttgcca cctggattct gcgcgggacg tccttctgct acgtcccttc
8101 ggccctcaat ccagcggacc ttccttcccg cggcctgctg ccggctctgc ggcctcttcc
8161 gcgtcttcgc cttcgccctc agacgagtcg gatctccctt tgggccgcct ccccgcatcg
8221 ataccgtcga cctcgatcga gacctagaaa aacatggagc aatcacaagt agcaatacag
8281 cagctaccaa tgctgattgt gcctggctag aagcacaaga ggaggaggag gtgggttttc
8341 cagtcacacc tcaggtacct ttaagaccaa tgacttacaa ggcagctgta gatcttagcc
8401 actttttaaa agaaaagggg ggactggaag ggctaattca ctcccaacga agacaagata
8461 tccttgatct gtggatctac cacacacaag gctacttccc tgattggcag aactacacac
8521 cagggccagg gatcagatat ccactgacct ttggatggtg ctacaagcta gtaccagttg
8581 agcaagagaa ggtagaagaa gccaatgaag gagagaacac ccgcttgtta caccctgtga
8641 gcctgcatgg gatggatgac ccggagagag aagtattaga gtggaggttt gacagccgcc
8701 tagcatttca tcacatggcc cgagagctgc atccggactg tactgggtct ctctggttag
8761 accagatctg agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat
8821 aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact
8881 agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagcatg tgagcaaaag
8941 gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc
9001 gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag
9061 gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga
9121 ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc
9181 atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg
9241 tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt
9301 ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca
9361 gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca
9421 ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag
9481 ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca
9541 agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg
9601 ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa
9661 aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta
9721 tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag
9781 cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga
9841 tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac
9901 cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc
9961 ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta
10021 gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac
10081 gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat
10141 gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa
10201 gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg
10261 tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag
10321 aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc
10381 cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct
10441 caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat
10501 cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg
10561 ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc
10621 aatattattg aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta
10681 tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgac
//
