| 质粒名称: | pcDNA3-eGFP-NLS-RNF168 |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 133977 |
| 存储实验室: | Daniel Durocher |
|
载体骨架基本信息 |
|
| 载体名称: | pcDNA3 |
| 载体出品公司: | Invitrogen |
| 载体抗性: | 氨苄青霉素 |
| 空载体大小: | 6126 bp |
| 完整质粒大小: | 7839 bp |
| 载体修饰: | -- |
| 载体类型: | 哺乳细胞表达 |
| 筛选标记: | -- |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | RNF168 |
| 物种来源: | H. sapiens (human) |
| 插入大小: | 1713 bp |
| 基因突变: | siRNA resistant sequence: GAGGAGTCGTGTTTATTGA |
| GenBank编号 | -- |
| 启动子 | CMV |
| 标签/融合蛋白: | eGFP（插入件上的N端子） |
| 克隆方法: | 限制性内切酶 |
| 5' 克隆位点: | AscI(未破坏) |
| 3' 克隆位点: | XbaI (未破坏) |
| 5' 测序引物 | CMV-F |
| 3' 测序引物 | BGH-rev |
|
文献引用方法 |
|
| 材料和方法部分: | pcDNA3-eGFP-NLS-RNF168 was a gift from Daniel Durocher (Addgene plasmid # 133977 ; http://n2t.net/addgene:133977 ; RRID:Addgene_133977) |
| 参考文献部分: | The RIDDLE syndrome protein mediates a ubiquitin-dependent signaling cascade at sites of DNA damage. Stewart GS, Panier S, Townsend K, Al-Hakim AK, Kolas NK, Miller ES, Nakada S, Ylanko J, Olivarius S, Mendez M, Oldreive C, Wildenhain J, Tagliaferro A, Pelletier L, Taubenheim N, Durandy A, Byrd PJ, Stankovic T, Taylor AM, Durocher D. Cell. 2009 Feb 6;136(3):420-34. doi: 10.1016/j.cell.2008.12.042. 10.1016/j.cell.2008.12.042 PubMed 19203578 |
买家导航
pcDNA3-eGFP-NLS-RNF168载体质粒基本信息
pcDNA3-eGFP-NLS-RNF168质粒图谱载体图谱和pcDNA3-eGFP-NLS-RNF168载体序列质粒序列多克隆位点信息
pcDNA3-eGFP-NLS-RNF168质粒载体简介
pcDNA3-eGFP-NLS-RNF168质粒序列载体序列
LOCUS pcDNA3-eGFP-NLS-RNF168 7838 bp ds-DNA circular SYN 12-MAY-2021
DEFINITION mammalian expression vector of eGFP tagged wild type RNF168 .
ACCESSION .
VERSION .
KEYWORDS pcDNA3-eGFP-NLS-RNF168
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 7838)
AUTHORS Stewart GS, Panier S, Townsend K, Al-Hakim AK, Kolas NK, Miller ES,
Nakada S, Ylanko J, Olivarius S, Mendez M, Oldreive C, Wildenhain J,
Tagliaferro A, Pelletier L, Taubenheim N, Durandy A, Byrd PJ,
Stankovic T, Taylor AM, Durocher D
TITLE The RIDDLE syndrome protein mediates a ubiquitin-dependent signaling
cascade at sites of DNA damage.
JOURNAL Cell. 2009 Feb 6;136(3):420-34. doi: 10.1016/j.cell.2008.12.042.
PUBMED 19203578
REFERENCE 2 (bases 1 to 7838)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..7838
/organism="synthetic DNA construct"
/mol_type="other DNA"
enhancer 96..475
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 476..679
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind 630..650
/label=CMV-F
/note="Human CMV immediate early promoter, forward primer"
primer_bind 724..743
/label=T7
/note="T7 promoter, forward primer"
promoter 724..742
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
CDS 762..1478
/codon_start=1
/product="enhanced GFP"
/label=EGFP
/translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
EFVTAAGITLGMDELYK"
CDS 762..1478
/codon_start=1
/product="the original enhanced GFP (Yang et al., 1996)"
/label=EGFP
/note="mammalian codon-optimized"
/translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
EFVTAAGITLGMDELYK"
primer_bind complement(807..828)
/label=EGFP-N
/note="EGFP, reverse primer"
primer_bind complement(1068..1087)
/label=EXFP-R
/note="For distinguishing EGFP variants, reverse primer"
primer_bind 1415..1436
/label=EGFP-C
/note="EGFP, forward primer"
CDS 1488..1508
/codon_start=1
/product="nuclear localization signal of SV40 (simian virus
40) large T antigen"
/label=SV40 NLS
/translation="PKKKRKV"
CDS 3008..3019
/codon_start=1
/product="WELQut protease recognition and cleavage site"
/label=WELQut site
/translation="WELQ"
promoter complement(3252..3270)
/label=SP6 promoter
/note="promoter for bacteriophage SP6 RNA polymerase"
primer_bind complement(3253..3270)
/label=SP6
/note="SP6 promoter, forward primer"
primer_bind complement(3290..3307)
/label=BGH-rev
/note="Bovine growth hormone terminator, reverse primer.
Also called BGH reverse"
polyA_signal 3296..3520
/label=bGH poly(A) signal
/note="bovine growth hormone polyadenylation signal"
rep_origin 3566..3994
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(3653..3672)
/label=F1ori-R
/note="F1 origin, reverse primer"
primer_bind 3863..3884
/label=F1ori-F
/note="F1 origin, forward primer"
primer_bind complement(4003..4023)
/label=pBABE 3'
/note="SV40 enhancer, reverse primer for pBABE vectors"
promoter 4008..4337
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
rep_origin 4188..4323
/label=SV40 ori
/note="SV40 origin of replication"
primer_bind 4250..4269
/label=SV40pro-F
/note="SV40 promoter/origin, forward primer"
CDS 4404..5198
/codon_start=1
/gene="aph(3')-II (or nptII)"
/product="aminoglycoside phosphotransferase from Tn5"
/label=NeoR/KanR
/note="confers resistance to neomycin, kanamycin, and G418
(Geneticin(R))"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
primer_bind complement(4458..4477)
/label=Neo-R
/note="Neomycin resistance gene, reverse primer"
primer_bind 5068..5087
/label=Neo-F
/note="Neomycin resistance gene, forward primer"
polyA_signal 5372..5493
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(5409..5428)
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind 5463..5482
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
primer_bind complement(5542..5558)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(5542..5558)
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind complement(5555..5577)
/label=M13/pUC Reverse
/note="In lacZ gene"
protein_bind 5566..5582
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(5590..5620)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 5635..5656
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
primer_bind complement(5773..5790)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(5944..6532)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(6024..6043)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(6703..7563)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 7326..7345
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(7564..7668)
/gene="bla"
/label=AmpR promoter
primer_bind complement(7743..7762)
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
ORIGIN
1 aacaaggcaa ggcttgaccg acaattgcat gaagaatctg cttagggtta ggcgttttgc
61 gctgcttcgc gatgtacggg ccagatatac gcgttgacat tgattattga ctagttatta
121 atagtaatca attacggggt cattagttca tagcccatat atggagttcc gcgttacata
181 acttacggta aatggcccgc ctggctgacc gcccaacgac ccccgcccat tgacgtcaat
241 aatgacgtat gttcccatag taacgccaat agggactttc cattgacgtc aatgggtgga
301 gtatttacgg taaactgccc acttggcagt acatcaagtg tatcatatgc caagtacgcc
361 ccctattgac gtcaatgacg gtaaatggcc cgcctggcat tatgcccagt acatgacctt
421 atgggacttt cctacttggc agtacatcta cgtattagtc atcgctatta ccatggtgat
481 gcggttttgg cagtacatca atgggcgtgg atagcggttt gactcacggg gatttccaag
541 tctccacccc attgacgtca atgggagttt gttttggcac caaaatcaac gggactttcc
601 aaaatgtcgt aacaactccg ccccattgac gcaaatgggc ggtaggcgtg tacggtggga
661 ggtctatata agcagagctc tctggctaac tagagaaccc actgcttact ggcttatcga
721 aattaatacg actcactata gggagaccca agcttggtac catggtgagc aagggcgagg
781 agctgttcac cggggtggtg cccatcctgg tcgagctgga cggcgacgta aacggccaca
841 agttcagcgt gtccggcgag ggcgagggcg atgccaccta cggcaagctg accctgaagt
901 tcatctgcac caccggcaag ctgcccgtgc cctggcccac cctcgtgacc accctgacct
961 acggcgtgca gtgcttcagc cgctaccccg accacatgaa gcagcacgac ttcttcaagt
1021 ccgccatgcc cgaaggctac gtccaggagc gcaccatctt cttcaaggac gacggcaact
1081 acaagacccg cgccgaggtg aagttcgagg gcgacaccct ggtgaaccgc atcgagctga
1141 agggcatcga cttcaaggag gacggcaaca tcctggggca caagctggag tacaactaca
1201 acagccacaa cgtctatatc atggccgaca agcagaagaa cggcatcaag gtgaacttca
1261 agatccgcca caacatcgag gacggcagcg tgcagctcgc cgaccactac cagcagaaca
1321 cccccatcgg cgacggcccc gtgctgctgc ccgacaacca ctacctgagc acccagtccg
1381 ccctgagcaa agaccccaac gagaagcgcg atcacatggt cctgctggag ttcgtgaccg
1441 ccgccgggat cactctcggc atggacgagc tgtacaaggg cgcgggaccc aagaagaagc
1501 ggaaggtggg agggcgcgcc atggctctac ccaaagacgc catcccctcg ctgtccgagt
1561 gccagtgcgg gatctgcatg gaaatccttg tggagcccgt caccctcccg tgtaaccaca
1621 cgctgtgtaa accgtgcttc cagtcgaccg tcgaaaaggc gagtttatgc tgtcccttct
1681 gtcgccgccg ggtatcgtcg tggactcggt accatacccg aagaaattct ctcgtcaacg
1741 tggaactgtg gacgataatt caaaaacact atcccaggga gtgcaagctt agagcgtctg
1801 gccaagaatc agaggaagtg gctgatgact atcagccagt tcgtctgctc agtaaacctg
1861 gggaactgag aagagaatat gaagaggaaa taagcaaggt ggcggcagag cgacgggcca
1921 gcgaggaaga agaaaacaaa gccagtgaag aatacataca gaggttgttg gcagaggagg
1981 aagaagagga aaaaagacag gcagaaaaaa ggcgaagagc gatggaagaa caactgaaaa
2041 gtgatgagga actggcaaga aagctaagca ttgatattaa caatttctgt gagggaagta
2101 tctcggcttc tcccttgaat tccagaaaat ctgatccagt tacacccaag tctgagaaga
2161 aaagtaagaa caaacaaaga aacactggag atattcagaa gtatttgaca ccgaaatctc
2221 agtttgggtc agcctcacac tctgaagctg tacaagaagt caggaaagac tccgtatcta
2281 aggacattga cagtagtgat aggaaaagcc caacagggca agacacagaa atagaagata
2341 tgccgacact ttctccacag atatcccttg gagttggaga acaaggtgca gattcttcaa
2401 tagagtcccc tatgccatgg ttatgtgcct gtggtgccga atggtaccat gaaggaaacg
2461 tcaaaacaag accaagcaat catgggaaag agttatgtgt cttaagtcac gagcgaccta
2521 aaaccagagt tccctactcg aaagaaactg cagttatgcc ttgtggcaga acagaaagtg
2581 ggtgcgcccc cacatcaggg gtgacacaga caaatggaaa caacacaggt gagacagaaa
2641 atgaggagtc gtgtttattg atcagtaagg agatttccaa aagaaaaaac caagaatctt
2701 cctttgaagc agtcaaggat ccatgctttt ctgcaaaaag aagaaaagtg tcccccgaat
2761 cttccccaga tcaagaggaa acagaaataa actttaccca aaaactgata gatttggagc
2821 atctactgtt tgagagacat aaacaagaag aacaggacag gttattggca ttacaacttc
2881 agaaggaggt ggataaagag caaatggtgc caaaccggca aaaaggatcc ccagatgagt
2941 atcacttacg cgctacatcc tcccctccag acaaagtgct aaatggacag aggaagaatc
3001 ccaaagatgg gaacttcaaa aggcaaactc acacaaagca tccaacacca gagagaggct
3061 caagggacaa aaataggcaa gtgtctttaa agatgcagtt gaagcagtca gttaatagaa
3121 gaaagatgcc aaattctact agagatcact gtaaggtatc aaaaagtgct cactccctac
3181 agcctagcat ttcacagaaa agtgtttttc agatgtttca gagatgcaca aagtaatcta
3241 gagggcccta ttctatagtg tcacctaaat gctagagctc gctgatcagc ctcgactgtg
3301 ccttctagtt gccagccatc tgttgtttgc ccctcccccg tgccttcctt gaccctggaa
3361 ggtgccactc ccactgtcct ttcctaataa aatgaggaaa ttgcatcgca ttgtctgagt
3421 aggtgtcatt ctattctggg gggtggggtg gggcaggaca gcaaggggga ggattgggaa
3481 gacaatagca ggcatgctgg ggatgcggtg ggctctatgg cttctgaggc ggaaagaacc
3541 agctggggct ctagggggta tccccacgcg ccctgtagcg gcgcattaag cgcggcgggt
3601 gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc
3661 gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg
3721 gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat
3781 tagggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg
3841 ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaaccct
3901 atctcggtct attcttttga tttataaggg attttgccga tttcggccta ttggttaaaa
3961 aatgagctga tttaacaaaa atttaacgcg aattaattct gtggaatgtg tgtcagttag
4021 ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt
4081 agtcagcaac caggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca
4141 tgcatctcaa ttagtcagca accatagtcc cgcccctaac tccgcccatc ccgcccctaa
4201 ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag
4261 aggccgaggc cgcctctgcc tctgagctat tccagaagta gtgaggaggc ttttttggag
4321 gcctaggctt ttgcaaaaag ctcccgggag cttgtatatc cattttcgga tctgatcaag
4381 agacaggatg aggatcgttt cgcatgattg aacaagatgg attgcacgca ggttctccgg
4441 ccgcttgggt ggagaggcta ttcggctatg actgggcaca acagacaatc ggctgctctg
4501 atgccgccgt gttccggctg tcagcgcagg ggcgcccggt tctttttgtc aagaccgacc
4561 tgtccggtgc cctgaatgaa ctgcaggacg aggcagcgcg gctatcgtgg ctggccacga
4621 cgggcgttcc ttgcgcagct gtgctcgacg ttgtcactga agcgggaagg gactggctgc
4681 tattgggcga agtgccgggg caggatctcc tgtcatctca ccttgctcct gccgagaaag
4741 tatccatcat ggctgatgca atgcggcggc tgcatacgct tgatccggct acctgcccat
4801 tcgaccacca agcgaaacat cgcatcgagc gagcacgtac tcggatggaa gccggtcttg
4861 tcgatcagga tgatctggac gaagagcatc aggggctcgc gccagccgaa ctgttcgcca
4921 ggctcaaggc gcgcatgccc gacggcgagg atctcgtcgt gacccatggc gatgcctgct
4981 tgccgaatat catggtggaa aatggccgct tttctggatt catcgactgt ggccggctgg
5041 gtgtggcgga ccgctatcag gacatagcgt tggctacccg tgatattgct gaagagcttg
5101 gcggcgaatg ggctgaccgc ttcctcgtgc tttacggtat cgccgctccc gattcgcagc
5161 gcatcgcctt ctatcgcctt cttgacgagt tcttctgagc gggactctgg ggttcgaaat
5221 gaccgaccaa gcgacgccca acctgccatc acgagatttc gattccaccg ccgccttcta
5281 tgaaaggttg ggcttcggaa tcgttttccg ggacgccggc tggatgatcc tccagcgcgg
5341 ggatctcatg ctggagttct tcgcccaccc caacttgttt attgcagctt ataatggtta
5401 caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag
5461 ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tgtataccgt cgacctctag
5521 ctagagcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac
5581 aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt
5641 gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc
5701 gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg
5761 ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt
5821 atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa
5881 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc
5941 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag
6001 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt
6061 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg
6121 aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg
6181 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg
6241 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac
6301 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg
6361 gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt
6421 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg
6481 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc
6541 tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt
6601 ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt
6661 taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag
6721 tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt
6781 cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg caatgatacc
6841 gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc
6901 cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg
6961 ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac
7021 aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg
7081 atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc
7141 tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact
7201 gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc
7261 aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat
7321 acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc
7381 ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac
7441 tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa
7501 aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact
7561 catactcttc ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg
7621 atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg
7681 aaaagtgcca cctgacgtcg acggatcggg agatctcccg atcccctatg gtgcactctc
7741 agtacaatct gctctgatgc cgcatagtta agccagtatc tgctccctgc ttgtgtgttg
7801 gaggtcgctg agtagtgcgc gagcaaaatt taagctac
//
