| 质粒名称: | AbVec2.0-IGHG4 |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 99578 |
| 存储实验室: | Hedda Wardemann |
|
载体骨架基本信息 |
|
| 载体名称: | pUC19 |
| 空载体大小: | 5732 bp |
| 完整质粒大小: | 4989 bp |
| 载体类型: | 哺乳细胞表达 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5a |
| 高拷贝/低拷贝: | 高拷贝 |
|
插入基因信息 |
|
| 启动子: | hCMV IE1 gene promoter |
| 克隆方法: | 限制性内切酶 |
| 5’测序引物 | GCTTCGTTAGAACGCGGCTAC |
|
文献引用方法 |
|
| 材料和方法部分: | AbVec2.0-IGHG4 was a gift from Hedda Wardemann (Addgene plasmid # 99578 ; http://n2t.net/addgene:99578 ; RRID:Addgene_99578) |
| 参考文献部分: | 未发表 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS AbVec2.0-IGHG4 5732 bp ds-DNA circular SYN 12-MAY-2021
DEFINITION Expression of secretory immunoglobulin heavy chains in mammalian
cells, human IgG4 isotype.
ACCESSION .
VERSION .
KEYWORDS AbVec2.0-IGHG4
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 5732)
TITLE Immunoglobulin expression vectors
JOURNAL Unpublished
REFERENCE 2 (bases 1 to 5732)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58
http://www.biofeng.com
FEATURES Location/Qualifiers
source 1..5732
/organism="synthetic DNA construct"
/mol_type="other DNA"
protein_bind 103..124
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
promoter 139..169
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 177..193
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
primer_bind 182..204
/label=M13/pUC Reverse
/note="In lacZ gene"
primer_bind 201..217
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind 201..217
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
enhancer 246..625
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 626..829
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind 780..800
/label=CMV-F
/note="Human CMV immediate early promoter, forward primer"
primer_bind 826..850
/label=LNCX
/note="Human CMV promoter, forward primer"
promoter 1060..1078
/label=SP6 promoter
/note="promoter for bacteriophage SP6 RNA polymerase"
primer_bind 1060..1077
/label=SP6
/note="SP6 promoter, forward primer"
primer_bind 1091..1110
/label=pMT2-F
/note="Synthetic intron, forward primer"
regulatory 2264..2273
/regulatory_class="other"
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
polyA_signal 2277..2411
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(2314..2333)
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind 2368..2387
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
promoter 2480..2837
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
primer_bind complement(2503..2523)
/label=pBABE 3'
/note="SV40 enhancer, reverse primer for pBABE vectors"
rep_origin 2688..2823
/label=SV40 ori
/note="SV40 origin of replication"
primer_bind 2750..2769
/label=SV40pro-F
/note="SV40 promoter/origin, forward primer"
primer_bind complement(2857..2874)
/label=M13 Forward
/note="In lacZ gene. Also called M13-F20 or M13 (-21)
Forward"
primer_bind complement(2857..2873)
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(2866..2888)
/label=M13/pUC Forward
/note="In lacZ gene"
rep_origin 3086..3541
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(3173..3192)
/label=F1ori-R
/note="F1 origin, reverse primer"
primer_bind 3383..3404
/label=F1ori-F
/note="F1 origin, forward primer"
primer_bind complement(3558..3577)
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
primer_bind 3677..3699
/label=pGEX 3'
/note="pGEX vectors, reverse primer"
primer_bind complement(3737..3755)
/label=pBRforEco
/note="pBR322 vectors, upsteam of EcoRI site, forward
primer"
promoter 3823..3927
/gene="bla"
/label=AmpR promoter
CDS 3928..4788
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind complement(4146..4165)
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
rep_origin 4959..5547
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 5448..5467
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
primer_bind 5701..5718
/label=L4440
/note="L4440 vector, forward primer"
ORIGIN
1 cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatcca actggcacga
61 caggtttccc gactggaaag cgggcagtga gcgcaacgca attaatgtga gttagctcac
121 tcattaggca ccccaggctt tacactttat gcttccggct cgtatgttgt gtggaattgt
181 gagcggataa caatttcaca caggaaacag ctatgacatg attacgaatt aattcgagct
241 cgcccgacat tgattattga ctagttatta atagtaatca attacggggt cattagttca
301 tagcccatat atggagttcc gcgttacata acttacggta aatggcccgc ctggctgacc
361 gcccaacgac ccccgcccat tgacgtcaat aatgacgtat gttcccatag taacgccaat
421 agggactttc cattgacgtc aatgggtgga gtatttacgg taaactgccc acttggcagt
481 acatcaagtg tatcatatgc caagtacgcc ccctattgac gtcaatgacg gtaaatggcc
541 cgcctggcat tatgcccagt acatgacctt atgggacttt cctacttggc agtacatcta
601 cgtattagtc atcgctatta ccatggtgat gcggttttgg cagtacatca atgggcgtgg
661 atagcggttt gactcacggg gatttccaag tctccacccc attgacgtca atgggagttt
721 gttttggcac caaaatcaac gggactttcc aaaatgtcgt aacaactccg ccccattgac
781 gcaaatgggc ggtaggcgtg tacggtggga ggtctatata agcagagctc gtttagtgaa
841 ccgtcagatc gcctggagac gccatccacg ctgttttgac ctccatagaa gacaccggga
901 ccgatccagc ctccgcggcc gggaacggtg cattggaacg cggattcccc gtgccaagag
961 tgacgtaagt accgcctata gagtctatag gcccaccccc ttggcttcgt tagaacgcgg
1021 ctacaattaa tacataacct tatgtatcat acacatacga tttaggtgac actatagaat
1081 aacatccact ttgcctttct ctccacaggt gtccactccc aggtccaact gcacctcggt
1141 tctatcgatt gaattccacc atgggatggt catgtatcat cctttttcta gtagcaactg
1201 caaccggttg actaactagc tggtcgacca agggcccaag cgtcttcccc ctggcgccct
1261 gctccaggag cacctccgag agcacagccg ccctgggctg cctggtcaag gactacttcc
1321 ccgagccggt gacggtgtcg tggaactcag gcgccctgac cagcggcgtg cacaccttcc
1381 cggctgtcct acagtcctca ggactctact ccctcagcag cgtggtgacc gtgccctcca
1441 gcagcttggg cacgaagacc tacacctgca acgtagatca caagcccagc aacaccaagg
1501 tggacaagag agttgagtcc aaatatggtc ccccatgccc atcatgccca gcacctgagt
1561 tcctgggggg accatcagtc ttcctgttcc ccccaaaacc caaggacact ctcatgatct
1621 cccggacccc tgaggtcacg tgcgtggtgg tggacgtgag ccaggaagac cccgaggtcc
1681 agttcaactg gtacgtggat ggcgtggagg tgcataatgc caagacaaag ccgcgggagg
1741 agcagttcaa cagcacgtac cgtgtggtca gcgtcctcac cgtcctgcac caggactggc
1801 tgaacggcaa ggagtacaag tgcaaggtct ccaacaaagg cctcccgtcc tccatcgaga
1861 aaaccatctc caaagccaaa gggcagcccc gagagccaca ggtgtacacc ctgcccccat
1921 cccaggagga gatgaccaag aaccaggtca gcctgacctg cctggtcaaa ggcttctacc
1981 ccagcgacat cgccgtggag tgggagagca atgggcagcc ggagaacaac tacaagacca
2041 cgcctcccgt gctggactcc gacggctcct tcttcctcta cagcaggcta accgtggaca
2101 agagcaggtg gcaggagggg aatgtcttct catgctccgt gatgcatgag gctctgcaca
2161 accactacac acagaagagc ctctccctgt ctctgggtaa atgagtgcca gggccggcaa
2221 gcccccgctc cccgggctct cggggtcgcg cgaggaaagc ttggccgcca tggcccaact
2281 tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata
2341 aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc
2401 atgtctggat cgggaattaa ttcggcgcag caccatggcc tgaaataacc tctgaaagag
2461 gaacttggtt aggtaccttc tgaggcggaa agaaccatct gtggaatgtg tgtcagttag
2521 ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt
2581 agtcagcaac caggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca
2641 tgcatctcaa ttagtcagca accatagtcc cgcccctaac tccgcccatc ccgcccctaa
2701 ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag
2761 aggccgaggc cgcctcggcc tctgagctat tccagaagta gtgaggaggc ttttttggag
2821 gcctaggctt ttgcaaaaag ctgttaacag cttggcactg gccgtcgttt tacaacgtcg
2881 tgactgggaa aaccctggcg ttacccaact taatcgcctt gcagcacatc cccctttcgc
2941 cagttggcgt aatagcgaag aggcccgcac cgatcgccct tcccaacagt tgcgcagcct
3001 gaatggcgaa tggcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca
3061 ccgcatacgt caaagcaacc atagtacgcg ccctgtagcg gcgcattaag cgcggcgggt
3121 gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc
3181 gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg
3241 gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat
3301 ttgggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg
3361 ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaaccct
3421 atctcgggct attcttttga tttataaggg attttgccga tttcggccta ttggttaaaa
3481 aatgagctga tttaacaaaa atttaacgcg aattttaaca aaatattaac gtttacaatt
3541 ttatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gccccgacac
3601 ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga
3661 caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa
3721 cgcgcgagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata
3781 atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt
3841 ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg
3901 cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt
3961 cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta
4021 aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc
4081 ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa
4141 gttctgctat gtggcgcggt attatcccgt attgacgccg ggcaagagca actcggtcgc
4201 cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt
4261 acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact
4321 gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac
4381 aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata
4441 ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta
4501 ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg
4561 gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat
4621 aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt
4681 aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga
4741 aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa
4801 gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag
4861 gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac
4921 tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc
4981 gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat
5041 caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat
5101 actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct
5161 acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt
5221 cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg
5281 gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta
5341 cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg
5401 gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg
5461 tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc
5521 tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg
5581 gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat
5641 aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc
5701 agcgagtcag tgagcgagga agcggaagag cg
//
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