| 质粒名称: | EGFP-Rubicon |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 28022 |
| 存储实验室: | Qing Zhong |
|
载体骨架基本信息 |
|
| 载体名称: | pcDNA6/TO |
| 载体出品公司: | Invitrogen |
| 空载体大小: | 5078 bp |
| 完整质粒大小: | 8730 bp |
| 载体类型: | 哺乳细胞表达 |
| 筛选标记: | Zeocin |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5a |
| 拷贝数: | 高拷贝 |
|
插入基因信息 |
|
| 插入基因名称: | Rubicon |
| 物种来源: | H. sapiens (human) |
| 插入大小: | 2783 bp |
| 标签/融合蛋白: |
EGFP (N terminal on insert)
3xFlag (C terminal on insert)
|
| 克隆方法: | 限制性内切酶 |
| 5’测序引物 | CMV-F |
| 3’测序引物 | BGH-rev |
|
文献引用方法 |
|
| 材料和方法部分: |
EGFP-Rubicon was a gift from Qing Zhong (Addgene plasmid # 28022 ; http://n2t.net/addgene:28022 ; RRID:Addgene_28022)
|
| 参考文献部分: | The RUN domain of rubicon is important for hvps34 binding, lipid kinase inhibition, and autophagy suppression. Sun Q, Zhang J, Fan W, Wong KN, Ding X, Chen S, Zhong Q. J Biol Chem. 2011 Jan 7. 286(1):185-91. 10.1074/jbc.M110.126425 PubMed 21062745 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS EGFP-Rubicon 8730 bp ds-DNA circular SYN 12-MAY-2021
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS EGFP-Rubicon
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 8730)
AUTHORS Sun Q, Zhang J, Fan W, Wong KN, Ding X, Chen S, Zhong Q
TITLE The RUN domain of rubicon is important for hvps34 binding, lipid
kinase inhibition, and autophagy suppression.
JOURNAL J Biol Chem. 2011 Jan 7. 286(1):185-91.
PUBMED 21062745
REFERENCE 2 (bases 1 to 8730)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58
http://www.biofeng.com
FEATURES Location/Qualifiers
source 1..8730
/organism="synthetic DNA construct"
/mol_type="other DNA"
enhancer 110..489
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 490..693
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind 644..664
/label=CMV-F
/note="Human CMV immediate early promoter, forward primer"
protein_bind 695..713
/gene="tetO"
/label=tet operator
/bound_moiety="tetracycline repressor TetR"
/note="bacterial operator O2 for the tetR and tetA genes"
protein_bind 716..734
/gene="tetO"
/label=tet operator
/bound_moiety="tetracycline repressor TetR"
/note="bacterial operator O2 for the tetR and tetA genes"
primer_bind 744..768
/label=LNCX
/note="Human CMV promoter, forward primer"
CDS 859..1575
/codon_start=1
/product="enhanced GFP"
/label=EGFP
/translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
EFVTAAGITLGMDELYK"
CDS 859..1575
/codon_start=1
/product="the original enhanced GFP (Yang et al., 1996)"
/label=EGFP
/note="mammalian codon-optimized"
/translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
EFVTAAGITLGMDELYK"
primer_bind complement(904..925)
/label=EGFP-N
/note="EGFP, reverse primer"
primer_bind complement(1165..1184)
/label=EXFP-R
/note="For distinguishing EGFP variants, reverse primer"
primer_bind 1512..1533
/label=EGFP-C
/note="EGFP, forward primer"
CDS 4507..4572
/codon_start=1
/product="three tandem FLAG(R) epitope tags, followed by an
enterokinase cleavage site"
/label=3xFLAG
/translation="DYKDHDGDYKDHDIDYKDDDDK"
primer_bind complement(4613..4630)
/label=BGH-rev
/note="Bovine growth hormone terminator, reverse primer.
Also called BGH reverse"
polyA_signal 4619..4843
/label=bGH poly(A) signal
/note="bovine growth hormone polyadenylation signal"
rep_origin 4889..5317
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(4976..4995)
/label=F1ori-R
/note="F1 origin, reverse primer"
primer_bind 5186..5207
/label=F1ori-F
/note="F1 origin, forward primer"
primer_bind complement(5326..5346)
/label=pBABE 3'
/note="SV40 enhancer, reverse primer for pBABE vectors"
promoter 5331..5660
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
rep_origin 5511..5646
/label=SV40 ori
/note="SV40 origin of replication"
primer_bind 5573..5592
/label=SV40pro-F
/note="SV40 promoter/origin, forward primer"
promoter 5708..5755
/label=EM7 promoter
/note="synthetic bacterial promoter "
CDS 5774..6148
/codon_start=1
/gene="Sh ble from Streptoalloteichus hindustanus"
/product="antibiotic-binding protein"
/label=BleoR
/note="confers resistance to bleomycin, phleomycin, and
Zeocin(TM)"
/translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
EFALRDPAGNCVHFVAEEQD"
polyA_signal 6278..6399
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(6315..6334)
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind 6369..6388
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
primer_bind complement(6448..6464)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(6448..6464)
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind complement(6461..6483)
/label=M13/pUC Reverse
/note="In lacZ gene"
protein_bind 6472..6488
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(6496..6526)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 6541..6562
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
primer_bind complement(6679..6696)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(6850..7438)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(6930..6949)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(7609..8469)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 8232..8251
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(8470..8574)
/gene="bla"
/label=AmpR promoter
primer_bind complement(8649..8668)
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
ORIGIN
1 aaaatttaag ctacaacaag gcaaggcttg accgacaatt gcatgaagaa tctgcttagg
61 gttaggcgtt ttgcgctgct tcgcgatgta cgggccagat atacgcgttg acattgatta
121 ttgactagtt attaatagta atcaattacg gggtcattag ttcatagccc atatatggag
181 ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc
241 ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac tttccattga
301 cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat
361 atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc
421 cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct
481 attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca
541 cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat
601 caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg
661 cgtgtacggt gggaggtcta tataagcaga gctctcccta tcagtgatag agatctccct
721 atcagtgata gagatcgtcg acgagctcgt ttagtgaacc gtcagatcgc ctggagacgc
781 catccacgct gttttgacct ccatagaaga caccgggacc gatccagcct ccggactcta
841 gcgtttaaac ttaagcttat ggtgagcaag ggcgaggagc tgttcaccgg ggtggtgccc
901 atcctggtcg agctggacgg cgacgtaaac ggccacaagt tcagcgtgtc cggcgagggc
961 gagggcgatg ccacctacgg caagctgacc ctgaagttca tctgcaccac cggcaagctg
1021 cccgtgccct ggcccaccct cgtgaccacc ctgacctacg gcgtgcagtg cttcagccgc
1081 taccccgacc acatgaagca gcacgacttc ttcaagtccg ccatgcccga aggctacgtc
1141 caggagcgca ccatcttctt caaggacgac ggcaactaca agacccgcgc cgaggtgaag
1201 ttcgagggcg acaccctggt gaaccgcatc gagctgaagg gcatcgactt caaggaggac
1261 ggcaacatcc tggggcacaa gctggagtac aactacaaca gccacaacgt ctatatcatg
1321 gccgacaagc agaagaacgg catcaaggtg aacttcaaga tccgccacaa catcgaggac
1381 ggcagcgtgc agctcgccga ccactaccag cagaacaccc ccatcggcga cggccccgtg
1441 ctgctgcccg acaaccacta cctgagcacc cagtccgccc tgagcaaaga ccccaacgag
1501 aagcgcgatc acatggtcct gctggagttc gtgaccgccg ccgggatcac tctcggcatg
1561 gacgagctgt acaagggatc catgcggccg gagggcgcgg gaatggagct cggaggcggc
1621 gaggagcgcc tgcctgagga gagcaggagg gagcactggc agttgctggg taatttgaag
1681 acgacggtgg agggtttggt atcaaccaac agccccaacg tctggtctaa gtatggtggc
1741 ttggagcggc tttgcaggga catgcagagc atcctctatc acgggcttat ccgtgaccag
1801 gcgtgccgcc gccagacgga ttactggcag ttcgtgaaag acatccggtg gctcagtccc
1861 cactcagccc ttcacgtgga gaagttcatc agcgtgcacg agaacgacca gagcagtgct
1921 gatggtgcca gtgaacgtgc tgttgccgag ctgtggctgc agcacagcct gcagtaccac
1981 tgcctctcag cccagctccg gcccctgctc ggggatagac agtatatcag aaaattctac
2041 acagatgctg ccttcctgct aagtgacgct catgtcacgg ccatgctgca gtgcctggaa
2101 gccgtcgagc aaaataatcc ccgcctcctg gctcagatcg atgcgtccat gtttgccaga
2161 aagcacgaga gcccgctcct ggtgacaaag agccagagcc tgacagccct gcccagttcc
2221 acatacaccc ctccaaacag ctatgctcag cattcctact ttgggtcctt ctctagcctc
2281 caccaatccg tgcccaacaa tggctcagag agaagatcta cttcctttcc actctctggc
2341 cctccccgga aacctcaaga aagcagaggg cacgtctcac cagcagagga tcaaaccatc
2401 caagcccccc cagtttcagt ctctgcacta gccagggatt cccctttgac cccaaatgaa
2461 atgagctcca gtactctgac cagccccata gaggcatcct gggtcagcag ccagaatgat
2521 tccccaggtg atgccagtga ggggcctgag tacctggcca ttggcaactt ggacccccga
2581 ggccggactg ccagctgtca gagtcacagc agcaatgccg agagcagcag ttccaatttg
2641 ttctcctcca gcagctccca gaagccagat tctgctgcct cttccttagg ggaccaggaa
2701 ggaggtgggg agagccagct gtccagtgtc ctccgcaggt ccagcttctc agaggggcag
2761 acactcactg tcaccagtgg ggcaaagaaa agccacattc gctcccattc ggataccagc
2821 attgcctcca ggggagctcc agaatcctgc aatgataagg cgaagttgag aggccctttg
2881 ccctactctg gtcaaagcag tgaagtcagc acacccagct ctctgtacat ggaatatgaa
2941 ggtggtcggt acctgtgctc aggggaaggc atgttccgaa gaccatcaga aggacagtcc
3001 ctcatcagct acctctctga gcaagacttc ggcagctgtg ccgacctgga aaaggagaat
3061 gcccacttca gcatctcaga gtccttaatt gctgccatcg agctaatgaa gtgcaacatg
3121 atgagccagt gcctagagga ggaggaagtg gaagaggaag acagtgatag agagatccag
3181 gagctgaagc agaagatccg ccttcggcgc cagcaaatcc gcaccaagaa cctgctcccc
3241 atgtaccagg aggctgagca cggaagcttt cgggtcacct ccagcagctc ccagttcagc
3301 tcacgtgatt cggcacagct ctctgactct ggctctgctg atgaggttga tgaatttgaa
3361 atccaagatg ctgacatcag aaggaacaca gcctcaagca gcaaatcctt cgtttcctcc
3421 cagtccttct cccactgctt cctgcactcc acgtctgctg aggcggtggc catggggctc
3481 ctgaagcagt ttgaggggat gcagcttcca gccgcctcgg agctggagtg gcttgtcccg
3541 gagcatgatg cccctcagaa gctcctgccc attcctgact cactgcccat ctcaccggat
3601 gacgggcagc acgctgacat ctacaagctg cggattcgtg ttcgtggcaa cttggagtgg
3661 gccccgcccc ggcctcagat aatttttaat gttcatccag ccccaacgag gaaaattgcc
3721 gtggccaagc agaattaccg ctgtgcagga tgtggcatcc ggactgaccc tgattacatc
3781 aagcgactgc ggtactgtga gtacctgggc aagtacttct gccagtgctg ccacgagaat
3841 gcccagatgg ccatccccag ccgggttctg cgcaagtggg acttcagcaa gtactacgtc
3901 agcaacttct ccaaggacct gctcattaag atctggaatg atcctctctt caacgtgcag
3961 gacataaaca gtgccctcta taggaaggtc aagctgctca atcaagtccg gctgctgcgg
4021 gtccagctgt gtcacatgaa gaacatgttc aagacttgcc gactggccaa ggagcttctg
4081 gattcctttg acacagtccc aggccacctg acagaggacc tccacctgta ctcactgaat
4141 gacctgactg cgaccaggaa gggggagctg gggccccggc ttgctgagct caccagggca
4201 ggggctaccc atgtggagag atgcatgctc tgccaagcca aaggcttcat ctgtgagttc
4261 tgtcagaatg aggatgacat catctttccc tttgagctcc ataagtgccg gacctgtgaa
4321 gagtgtaaag cgtgttacca taaagcctgc ttcaagtctg gaagctgtcc gcgctgcgag
4381 cggctgcagg cccggcggga ggcactggcc aggcagagcc tggagtctta cctgtcagac
4441 tacgaggagg agcccgcgga agcgctggcc ctggaagccg ccgtcctgga ggccaccggc
4501 ggccgcgact acaaagacca tgacggtgat tataaagatc atgacatcga ctacaaggat
4561 gacgatgaca agtgactcga gtctagaggg cccgtttaaa cccgctgatc agcctcgact
4621 gtgccttcta gttgccagcc atctgttgtt tgcccctccc ccgtgccttc cttgaccctg
4681 gaaggtgcca ctcccactgt cctttcctaa taaaatgagg aaattgcatc gcattgtctg
4741 agtaggtgtc attctattct ggggggtggg gtggggcagg acagcaaggg ggaggattgg
4801 gaagacaata gcaggcatgc tggggatgcg gtgggctcta tggcttctga ggcggaaaga
4861 accagctggg gctctagggg gtatccccac gcgccctgta gcggcgcatt aagcgcggcg
4921 ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct
4981 ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat
5041 cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt
5101 gattagggtg atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg
5161 acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac
5221 cctatctcgg tctattcttt tgatttataa gggattttgc cgatttcggc ctattggtta
5281 aaaaatgagc tgatttaaca aaaatttaac gcgaattaat tctgtggaat gtgtgtcagt
5341 tagggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca
5401 attagtcagc aaccaggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa
5461 gcatgcatct caattagtca gcaaccatag tcccgcccct aactccgccc atcccgcccc
5521 taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg
5581 cagaggccga ggccgcctct gcctctgagc tattccagaa gtagtgagga ggcttttttg
5641 gaggcctagg cttttgcaaa aagctcccgg gagcttgtat atccattttc ggatctgatc
5701 agcacgtgtt gacaattaat catcggcata gtatatcggc atagtataat acgacaaggt
5761 gaggaactaa accatggcca agttgaccag tgccgttccg gtgctcaccg cgcgcgacgt
5821 cgccggagcg gtcgagttct ggaccgaccg gctcgggttc tcccgggact tcgtggagga
5881 cgacttcgcc ggtgtggtcc gggacgacgt gaccctgttc atcagcgcgg tccaggacca
5941 ggtggtgccg gacaacaccc tggcctgggt gtgggtgcgc ggcctggacg agctgtacgc
6001 cgagtggtcg gaggtcgtgt ccacgaactt ccgggacgcc tccgggccgg ccatgaccga
6061 gatcggcgag cagccgtggg ggcgggagtt cgccctgcgc gacccggccg gcaactgcgt
6121 gcacttcgtg gccgaggagc aggactgaca cgtgctacga gatttcgatt ccaccgccgc
6181 cttctatgaa aggttgggct tcggaatcgt tttccgggac gccggctgga tgatcctcca
6241 gcgcggggat ctcatgctgg agttcttcgc ccaccccaac ttgtttattg cagcttataa
6301 tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca
6361 ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctgta taccgtcgac
6421 ctctagctag agcttggcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc
6481 gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta
6541 atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa
6601 cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat
6661 tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg
6721 agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc
6781 aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt
6841 gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag
6901 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc
6961 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc
7021 ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt
7081 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt
7141 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc
7201 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa
7261 gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa
7321 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg
7381 tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga
7441 agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg
7501 gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg
7561 aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt
7621 aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact
7681 ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat
7741 gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg
7801 aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg
7861 ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat
7921 tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc
7981 ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt
8041 cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc
8101 agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga
8161 gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc
8221 gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa
8281 acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta
8341 acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg
8401 agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg
8461 aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat
8521 gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt
8581 tccccgaaaa gtgccacctg acgtcgacgg atcgggagat ctcccgatcc cctatggtgc
8641 actctcagta caatctgctc tgatgccgca tagttaagcc agtatctgct ccctgcttgt
8701 gtgttggagg tcgctgagta gtgcgcgagc
//
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