PinPoint Xa-3

价格:1350元

联系方式:I47-825O-882O

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载体基本信息

出品公司: Promega
载体名称: PinPoint Xa-3
质粒类型: 大肠杆菌表达载体;蛋白纯化
表达水平:
启动子: --
克隆方法: 多克隆位点,限制性内切酶
载体大小: 3333 bp
5' 测序引物及序列: --
3' 测序引物及序列: --
载体标签: --
载体抗性: Ampicillin
筛选标记: --
备注: --
产品目录号: V2061
稳定性: 瞬表达
组成型: 组成型
病毒/非病毒: 非病毒

载体质粒图谱和多克隆位点信息

Pin point xa1载体图谱
Pin Point Xa3多克隆位点

Pin Point Xa3载体特征

载体简介

载体序列

LOCUS       PinPoint Xa-3	3333 bp 	DNA	SYN
DEFINITION  PinPoint Xa-3
ACCESSION   
KEYWORDS    
SOURCE      
  ORGANISM  other sequences; artificial sequences; vectors.
FEATURES             Location/Qualifiers
     source          1..3333
                     /organism="PinPoint Xa-3"
                     /mol_type="other DNA"
     misc_feature    1..386
                     /label="biotin_Pinpoint_Xa-1"
     CDS             1..468
                     /label="ORF frame 1"
                     /translation="MKLKVTVNGTAYDVDVDVDKSHENPMGTILFGGGTGGAPAPAAG
                     GAGAGKAGEGEIPAPLAGTVSKILVKEGDTVKAGQTVLVLEAMKMETEINAPTDGKVE
                     KVLVKERDAVQGGQGLIKIGDLELIEGREKLQLGSGTDIRSPGAAAIWFYSVT*"
     misc_feature    complement(453..470)
                     /label="Sp6_primer"
                     /translation="MKLKVTVNGTAYDVDVDVDKSHENPMGTILFGGGTGGAPAPAAG
                     GAGAGKAGEGEIPAPLAGTVSKILVKEGDTVKAGQTVLVLEAMKMETEINAPTDGKVE
                     KVLVKERDAVQGGQGLIKIGDLELIEGREKLQLGSGTDIRSPGAAAIWFYSVT*"
     misc_feature    670..692
                     /label="pGEX_3_primer"
                     /translation="MKLKVTVNGTAYDVDVDVDKSHENPMGTILFGGGTGGAPAPAAG
                     GAGAGKAGEGEIPAPLAGTVSKILVKEGDTVKAGQTVLVLEAMKMETEINAPTDGKVE
                     KVLVKERDAVQGGQGLIKIGDLELIEGREKLQLGSGTDIRSPGAAAIWFYSVT*"
     promoter        851..879
                     /label="AmpR_promoter"
                     /translation="MKLKVTVNGTAYDVDVDVDKSHENPMGTILFGGGTGGAPAPAAG
                     GAGAGKAGEGEIPAPLAGTVSKILVKEGDTVKAGQTVLVLEAMKMETEINAPTDGKVE
                     KVLVKERDAVQGGQGLIKIGDLELIEGREKLQLGSGTDIRSPGAAAIWFYSVT*"
     gene            921..1781
                     /label="Ampicillin"
                     /gene="Ampicillin"
                     /translation="MKLKVTVNGTAYDVDVDVDKSHENPMGTILFGGGTGGAPAPAAG
                     GAGAGKAGEGEIPAPLAGTVSKILVKEGDTVKAGQTVLVLEAMKMETEINAPTDGKVE
                     KVLVKERDAVQGGQGLIKIGDLELIEGREKLQLGSGTDIRSPGAAAIWFYSVT*"
     CDS             921..1781
                     /label="ORF frame 3"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
     rep_origin      1936..2555
                     /label="pBR322_origin"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
     promoter        2798..2816
                     /label="T7_promoter"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
     misc_feature    2940..2959
                     /label="pBRrevBam_primer"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
     promoter        3211..3239
                     /label="tac_promoter"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
     misc_feature    3251..3273
                     /label="M13_pUC_rev_primer"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
ORIGIN
    1 atgaaactga aggtaacagt caacggcact gcgtatgacg ttgacgttga cgtcgacaag
   61 tcacacgaaa acccgatggg caccatcctg ttcggcggcg gcaccggcgg cgcgccggca
  121 ccggcagcag gtggcgcagg cgccggtaag gccggagagg gcgagattcc cgctccgctg
  181 gccggcaccg tctccaagat cctcgtgaag gagggtgaca cggtcaaggc tggtcagacc
  241 gtgctcgttc tcgaggccat gaagatggag accgagatca acgctcccac cgacggcaag
  301 gtcgagaagg tcctggtcaa ggagcgtgac gcggtgcagg gcggtcaggg tctcatcaag
  361 atcggggatc tcgagctcat cgaaggtcgc gaaaagcttc agctgggatc cggtaccgat
  421 atcagatctc ccggggcggc cgcgatctgg ttctatagtg tcacctaaat cgtatgtgta
  481 tgatacataa ggttatgtat taattgtagc cgcgttctaa cgacaatatg tccatatggt
  541 gcactctcag tacaatctgc tctgatgccg catagttaag ccagccccga cacccgccaa
  601 cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg
  661 tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga
  721 gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt
  781 cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt
  841 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
  901 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt
  961 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg
 1021 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga
 1081 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc
 1141 tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac
 1201 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg
 1261 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca
 1321 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg
 1381 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg
 1441 acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg
 1501 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag
 1561 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg
 1621 gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct
 1681 cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac
 1741 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact
 1801 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga
 1861 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
 1921 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
 1981 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
 2041 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc
 2101 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
 2161 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
 2221 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
 2281 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
 2341 agcattgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
 2401 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
 2461 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
 2521 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
 2581 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
 2641 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt
 2701 cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc
 2761 cgattcatta atgcaggtta acctggctta tcgaaattaa tacgactcac tatagggaga
 2821 ccggcctcga gcagcaagga gatggcgccc aacagtcccc cggccacggg gcctgccacc
 2881 atacccacgc cgaaacaagc gctcatgagc ccgaagtggc gagcccgatc ttccccatcg
 2941 gtgatgtcgg cgatataggc gccagcaacc gcacctgtgg cgccggtgat gccggccacg
 3001 atgcgtccgg cgtagaggat cgatccgggc ttatcgactg cacggtgcac caatgcttct
 3061 ggcgtcaggc agccatcgga agctgtggta tggctgtgca ggtcgtaaat cactgcataa
 3121 ttcgtgtcgc tcaaggcgca ctcccgttct ggataatgtt ttttgcgccg acatcataac
 3181 ggttctggca aatattctga aatgagctgt tgacaattaa tcatcggctc gtataatgtg
 3241 tggaattgtg agcggataac aatttcacac aggaaacaga attcccagct tggctgcaga
 3301 accattccat tcgttgatcc gggagtaact cac
//

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