买家导航
pAAV-IRES-ZsGreen1载体质粒基本信息
pAAV-IRES-ZsGreen1质粒图谱载体图谱和pAAV-IRES-ZsGreen1载体序列质粒序列多克隆位点信息
pAAV-IRES-ZsGreen1质粒载体简介
pAAV-IRES-ZsGreen1质粒序列载体序列
LOCUS pAAV-IRES-ZsGreen1 5904 bp DNA circular SYN 13-JUL-2023 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 5904) AUTHORS Biofeng TITLE Direct Submission JOURNAL Exported Jul 13, 2023 from SnapGene 6.0.2 https://www.biofeng.com FEATURES Location/Qualifiers source 1..5904 /mol_type="other DNA" /organism="synthetic DNA construct" repeat_region 1..141 /label=AAV2 ITR /note="inverted terminal repeat of adeno-associated virus serotype 2" enhancer 221..524 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 525..727 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" intron 876..1254 /label=chimeric intron /note="fusion between human cytomegalovirus intron A and human beta-globin intron 2" misc_feature 1345..1917 /label=IRES /note="internal ribosome entry site (IRES) of the encephalomyocarditis virus (EMCV)" misc_feature 1368..1919 /label=IRES /note="internal ribosome entry site (IRES) of the encephalomyocarditis virus (EMCV)" CDS 1919..2614 /codon_start=1 /product="Zoanthus green fluorescent protein" /label=ZsGreen1 /note="mammalian codon-optimized" /translation="MAQSKHGLTKEMTMKYRMEGCVDGHKFVITGEGIGYPFKGKQAIN LCVVEGGPLPFAEDILSAAFMYGNRVFTEYPQDIVDYFKNSCPAGYTWDRSFLFEDGAV CICNADITVSVEENCMYHESKFYGVNFPADGPVMKKMTDNWEPSCEKIIPVPKQGILKG DVSMYLLLKDGGRLRCQFDTVYKAKSVPRKMPDWHFIQHKLTREDRSDAKNQKWHLTEH AIASGSALP" polyA_signal 2651..3127 /label=hGH poly(A) signal /note="human growth hormone polyadenylation signal" repeat_region 3167..3307 /label=AAV2 ITR /note="inverted terminal repeat of adeno-associated virus serotype 2" rep_origin 3382..3837 /direction=RIGHT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" promoter 4119..4223 /gene="bla" /label=AmpR promoter CDS 4224..5084 /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" rep_origin 5255..5843 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" ORIGIN 1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc 61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca 121 actccatcac taggggttcc tgcggccgca cgcgtggagc tagttattaa tagtaatcaa 181 ttacggggtc attagttcat agcccatata tggagttccg cgttacataa cttacggtaa 241 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg 301 ttcccatagt aacgtcaata gggactttcc attgacgtca atgggtggag tatttacggt 361 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg 421 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc 481 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc 541 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca 601 ttgacgtcaa tgggagtttg ttttgcacca aaatcaacgg gactttccaa aatgtcgtaa 661 caactccgcc ccattgacgc aaatgggcgg taggcgtgta cggtgggagg tctatataag 721 cagagctcgt ttagtgaacc gtcagatcgc ctggagacgc catccacgct gttttgacct 781 ccatagaaga caccgggacc gatccagcct ccgcggattc gaatcccggc cgggaacggt 841 gcattggaac gcggattccc cgtgccaaga gtgacgtaag taccgcctat agagtctata 901 ggcccacaaa aaatgctttc ttcttttaat atactttttt gtttatctta tttctaatac 961 tttccctaat ctctttcttt cagggcaata atgatacaat gtatcatgcc tctttgcacc 1021 attctaaaga ataacagtga taatttctgg gttaaggcaa tagcaatatt tctgcatata 1081 aatatttctg catataaatt gtaactgatg taagaggttt catattgcta atagcagcta 1141 caatccagct accattctgc ttttatttta tggttgggat aaggctggat tattctgagt 1201 ccaagctagg cccttttgct aatcatgttc atacctctta tcttcctccc acagctcctg 1261 ggcaacgtgc tggtctgtgt gctggcccat cactttggca aagaattggg attcgaacat 1321 cgattgaatt ccccggggat cccgcccctc tccctccccc ccccctaacg ttactggccg 1381 aagccgcttg gaataaggcc ggtgtgcgtt tgtctatatg ttattttcca ccatattgcc 1441 gtcttttggc aatgtgaggg cccggaaacc tggccctgtc ttcttgacga gcattcctag 1501 gggtctttcc cctctcgcca aaggaatgca aggtctgttg aatgtcgtga aggaagcagt 1561 tcctctggaa gcttcttgaa gacaaacaac gtctgtagcg accctttgca ggcagcggaa 1621 ccccccacct ggcgacaggt gcctctgcgg ccaaaagcca cgtgtataag atacacctgc 1681 aaaggcggca caaccccagt gccacgttgt gagttggata gttgtggaaa gagtcaaatg 1741 gctcacctca agcgtattca acaaggggct gaaggatgcc cagaaggtac cccattgtat 1801 gggatctgat ctggggcctc ggtgcacatg ctttacatgt gtttagtcga ggttaaaaaa 1861 cgtctaggcc ccccgaacca cggggacgtg gttttccttt gaaaaacacg atgataatat 1921 ggcccagtcc aagcacggcc tgaccaagga gatgaccatg aagtaccgca tggagggctg 1981 cgtggacggc cacaagttcg tgatcaccgg cgagggcatc ggctacccct tcaagggcaa 2041 gcaggccatc aacctgtgcg tggtggaggg cggccccttg cccttcgccg aggacatctt 2101 gtccgccgcc ttcatgtacg gcaaccgcgt gttcaccgag tacccccagg acatcgtcga 2161 ctacttcaag aactcctgcc ccgccggcta cacctgggac cgctccttcc tgttcgagga 2221 cggcgccgtg tgcatctgca acgccgacat caccgtgagc gtggaggaga actgcatgta 2281 ccacgagtcc aagttctacg gcgtgaactt ccccgccgac ggccccgtga tgaagaagat 2341 gaccgacaac tgggagccct cctgcgagaa gatcatcccc gtgcccaagc agggcatctt 2401 gaagggcgac gtgagcatgt acctgctgct gaaggacggt ggccgcttgc gctgccagtt 2461 cgacaccgtg tacaaggcca agtccgtgcc ccgcaagatg cccgactggc acttcatcca 2521 gcacaagctg acccgcgagg accgcagcga cgccaagaac cagaagtggc acctgaccga 2581 gcacgccatc gcctccggct ccgccttgcc ctgaaagctt gcctcgagca gcgctgctcg 2641 agagatctac gggtggcatc cctgtgaccc ctccccagtg cctctcctgg ccctggaagt 2701 tgccactcca gtgcccacca gccttgtcct aataaaatta agttgcatca ttttgtctga 2761 ctaggtgtcc ttctataata ttatggggtg gaggggggtg gtatggagca aggggcaagt 2821 tgggaagaca acctgtaggg cctgcggggt ctattgggaa ccaagctgga gtgcagtggc 2881 acaatcttgg ctcactgcaa tctccgcctc ctgggttcaa gcgattctcc tgcctcagcc 2941 tcccgagttg ttgggattcc aggcatgcat gaccaggctc agctaatttt tgtttttttg 3001 gtagagacgg ggtttcacca tattggccag gctggtctcc aactcctaat ctcaggtgat 3061 ctacccacct tggcctccca aattgctggg attacaggcg tgaaccactg ctcccttccc 3121 tgtccttctg attttgtagg taaccacgtg cggaccgagc ggccgcagga acccctagtg 3181 atggagttgg ccactccctc tctgcgcgct cgctcgctca ctgaggccgg gcgaccaaag 3241 gtcgcccgac gcccgggctt tgcccgggcg gcctcagtga gcgagcgagc gcgcagctgc 3301 ctgcaggggc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc 3361 atacgtcaaa gcaaccatag tacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg 3421 tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt 3481 tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc 3541 tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgatttgg 3601 gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg 3661 agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct 3721 cgggctattc ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg 3781 agctgattta acaaaaattt aacgcgaatt ttaacaaaat attaacgttt acaattttat 3841 ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagccc cgacacccgc 3901 caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag 3961 ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg 4021 cgagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg 4081 tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat 4141 ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc 4201 aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct 4261 tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag 4321 atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta 4381 agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc 4441 tgctatgtgg cgcggtatta tcccgtattg acgccgggca agagcaactc ggtcgccgca 4501 tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg 4561 atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg 4621 ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca 4681 tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa 4741 acgacgagcg tgacaccacg atgcctgtag caatggcaac aacgttgcgc aaactattaa 4801 ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata 4861 aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat 4921 ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc 4981 cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata 5041 gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt 5101 actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga 5161 agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag 5221 cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa 5281 tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag 5341 agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg 5401 tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat 5461 acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta 5521 ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg 5581 gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc 5641 gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa 5701 gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc 5761 tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt 5821 caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct 5881 tttgctggcc ttttgctcac atgt //
pAAV-IRES-ZsGreen1载体图谱质粒图谱pdf版和pAAV-IRES-ZsGreen1载体序列质粒序列等相关资料下载
pAAV-IRES-ZsGreen1质粒载体应用举例
- 上一篇:pAAV-IRES-tdTomato
- 下一篇:ISceI-GR-RFP