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LOCUS pAAV-IRES-ZsGreen1 5904 bp DNA circular SYN 13-JUL-2023
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 5904)
AUTHORS Biofeng
TITLE Direct Submission
JOURNAL Exported Jul 13, 2023 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..5904
/mol_type="other DNA"
/organism="synthetic DNA construct"
repeat_region 1..141
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
enhancer 221..524
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 525..727
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
intron 876..1254
/label=chimeric intron
/note="fusion between human cytomegalovirus intron A and
human beta-globin intron 2"
misc_feature 1345..1917
/label=IRES
/note="internal ribosome entry site (IRES) of the
encephalomyocarditis virus (EMCV)"
misc_feature 1368..1919
/label=IRES
/note="internal ribosome entry site (IRES) of the
encephalomyocarditis virus (EMCV)"
CDS 1919..2614
/codon_start=1
/product="Zoanthus green fluorescent protein"
/label=ZsGreen1
/note="mammalian codon-optimized"
/translation="MAQSKHGLTKEMTMKYRMEGCVDGHKFVITGEGIGYPFKGKQAIN
LCVVEGGPLPFAEDILSAAFMYGNRVFTEYPQDIVDYFKNSCPAGYTWDRSFLFEDGAV
CICNADITVSVEENCMYHESKFYGVNFPADGPVMKKMTDNWEPSCEKIIPVPKQGILKG
DVSMYLLLKDGGRLRCQFDTVYKAKSVPRKMPDWHFIQHKLTREDRSDAKNQKWHLTEH
AIASGSALP"
polyA_signal 2651..3127
/label=hGH poly(A) signal
/note="human growth hormone polyadenylation signal"
repeat_region 3167..3307
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
rep_origin 3382..3837
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 4119..4223
/gene="bla"
/label=AmpR promoter
CDS 4224..5084
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 5255..5843
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc
61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca
121 actccatcac taggggttcc tgcggccgca cgcgtggagc tagttattaa tagtaatcaa
181 ttacggggtc attagttcat agcccatata tggagttccg cgttacataa cttacggtaa
241 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg
301 ttcccatagt aacgtcaata gggactttcc attgacgtca atgggtggag tatttacggt
361 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg
421 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc
481 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc
541 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca
601 ttgacgtcaa tgggagtttg ttttgcacca aaatcaacgg gactttccaa aatgtcgtaa
661 caactccgcc ccattgacgc aaatgggcgg taggcgtgta cggtgggagg tctatataag
721 cagagctcgt ttagtgaacc gtcagatcgc ctggagacgc catccacgct gttttgacct
781 ccatagaaga caccgggacc gatccagcct ccgcggattc gaatcccggc cgggaacggt
841 gcattggaac gcggattccc cgtgccaaga gtgacgtaag taccgcctat agagtctata
901 ggcccacaaa aaatgctttc ttcttttaat atactttttt gtttatctta tttctaatac
961 tttccctaat ctctttcttt cagggcaata atgatacaat gtatcatgcc tctttgcacc
1021 attctaaaga ataacagtga taatttctgg gttaaggcaa tagcaatatt tctgcatata
1081 aatatttctg catataaatt gtaactgatg taagaggttt catattgcta atagcagcta
1141 caatccagct accattctgc ttttatttta tggttgggat aaggctggat tattctgagt
1201 ccaagctagg cccttttgct aatcatgttc atacctctta tcttcctccc acagctcctg
1261 ggcaacgtgc tggtctgtgt gctggcccat cactttggca aagaattggg attcgaacat
1321 cgattgaatt ccccggggat cccgcccctc tccctccccc ccccctaacg ttactggccg
1381 aagccgcttg gaataaggcc ggtgtgcgtt tgtctatatg ttattttcca ccatattgcc
1441 gtcttttggc aatgtgaggg cccggaaacc tggccctgtc ttcttgacga gcattcctag
1501 gggtctttcc cctctcgcca aaggaatgca aggtctgttg aatgtcgtga aggaagcagt
1561 tcctctggaa gcttcttgaa gacaaacaac gtctgtagcg accctttgca ggcagcggaa
1621 ccccccacct ggcgacaggt gcctctgcgg ccaaaagcca cgtgtataag atacacctgc
1681 aaaggcggca caaccccagt gccacgttgt gagttggata gttgtggaaa gagtcaaatg
1741 gctcacctca agcgtattca acaaggggct gaaggatgcc cagaaggtac cccattgtat
1801 gggatctgat ctggggcctc ggtgcacatg ctttacatgt gtttagtcga ggttaaaaaa
1861 cgtctaggcc ccccgaacca cggggacgtg gttttccttt gaaaaacacg atgataatat
1921 ggcccagtcc aagcacggcc tgaccaagga gatgaccatg aagtaccgca tggagggctg
1981 cgtggacggc cacaagttcg tgatcaccgg cgagggcatc ggctacccct tcaagggcaa
2041 gcaggccatc aacctgtgcg tggtggaggg cggccccttg cccttcgccg aggacatctt
2101 gtccgccgcc ttcatgtacg gcaaccgcgt gttcaccgag tacccccagg acatcgtcga
2161 ctacttcaag aactcctgcc ccgccggcta cacctgggac cgctccttcc tgttcgagga
2221 cggcgccgtg tgcatctgca acgccgacat caccgtgagc gtggaggaga actgcatgta
2281 ccacgagtcc aagttctacg gcgtgaactt ccccgccgac ggccccgtga tgaagaagat
2341 gaccgacaac tgggagccct cctgcgagaa gatcatcccc gtgcccaagc agggcatctt
2401 gaagggcgac gtgagcatgt acctgctgct gaaggacggt ggccgcttgc gctgccagtt
2461 cgacaccgtg tacaaggcca agtccgtgcc ccgcaagatg cccgactggc acttcatcca
2521 gcacaagctg acccgcgagg accgcagcga cgccaagaac cagaagtggc acctgaccga
2581 gcacgccatc gcctccggct ccgccttgcc ctgaaagctt gcctcgagca gcgctgctcg
2641 agagatctac gggtggcatc cctgtgaccc ctccccagtg cctctcctgg ccctggaagt
2701 tgccactcca gtgcccacca gccttgtcct aataaaatta agttgcatca ttttgtctga
2761 ctaggtgtcc ttctataata ttatggggtg gaggggggtg gtatggagca aggggcaagt
2821 tgggaagaca acctgtaggg cctgcggggt ctattgggaa ccaagctgga gtgcagtggc
2881 acaatcttgg ctcactgcaa tctccgcctc ctgggttcaa gcgattctcc tgcctcagcc
2941 tcccgagttg ttgggattcc aggcatgcat gaccaggctc agctaatttt tgtttttttg
3001 gtagagacgg ggtttcacca tattggccag gctggtctcc aactcctaat ctcaggtgat
3061 ctacccacct tggcctccca aattgctggg attacaggcg tgaaccactg ctcccttccc
3121 tgtccttctg attttgtagg taaccacgtg cggaccgagc ggccgcagga acccctagtg
3181 atggagttgg ccactccctc tctgcgcgct cgctcgctca ctgaggccgg gcgaccaaag
3241 gtcgcccgac gcccgggctt tgcccgggcg gcctcagtga gcgagcgagc gcgcagctgc
3301 ctgcaggggc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc
3361 atacgtcaaa gcaaccatag tacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg
3421 tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt
3481 tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc
3541 tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgatttgg
3601 gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg
3661 agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct
3721 cgggctattc ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg
3781 agctgattta acaaaaattt aacgcgaatt ttaacaaaat attaacgttt acaattttat
3841 ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagccc cgacacccgc
3901 caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag
3961 ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg
4021 cgagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg
4081 tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat
4141 ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc
4201 aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct
4261 tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag
4321 atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta
4381 agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc
4441 tgctatgtgg cgcggtatta tcccgtattg acgccgggca agagcaactc ggtcgccgca
4501 tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg
4561 atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg
4621 ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca
4681 tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa
4741 acgacgagcg tgacaccacg atgcctgtag caatggcaac aacgttgcgc aaactattaa
4801 ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata
4861 aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat
4921 ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc
4981 cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata
5041 gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt
5101 actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga
5161 agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag
5221 cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa
5281 tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag
5341 agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg
5401 tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat
5461 acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta
5521 ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg
5581 gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc
5641 gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa
5701 gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc
5761 tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt
5821 caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct
5881 tttgctggcc ttttgctcac atgt
//
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