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pcDNA3.1+P2A载体质粒基本信息
pcDNA3.1+P2A质粒图谱载体图谱和pcDNA3.1+P2A载体序列质粒序列多克隆位点信息
pcDNA3.1+P2A质粒载体简介
pcDNA3.1+P2A质粒序列载体序列
LOCUS pcDNA3.1-P2A 5477 bp DNA circular SYN 30-MAR-2021
SOURCE
ORGANISM
COMMENT This file is created by Vector NTI
http://www.biofeng.com/
COMMENT ORIGDB|GenBank
COMMENT VNTDATE|-13802487|
COMMENT VNTDBDATE|-13802487|
COMMENT LSOWNER|
COMMENT VNTNAME|pcDNA3.1-P2A|
COMMENT VNTAUTHORNAME|Biofeng.com|
FEATURES Location/Qualifiers
source 1..5477
/invitrogen="124"
/vntifkey="98"
CDS complement(4481..5341)
/invitrogen="1020000"
/vntifkey="4"
/label=Amp(R)
polyA_site 1077..1301
/invitrogen="1870000"
/vntifkey="26"
/label=BGH\pA
primer complement(1071..1088)
/invitrogen="1910000"
/vntifkey="27"
/label=BGH\reverse\primer
promoter complement(5342..5440)
/invitrogen="2330000"
/vntifkey="30"
/label=bla\promoter
primer 769..789
/invitrogen="1930000"
/vntifkey="27"
/label=CMV\forward\primer
promoter 232..819
/invitrogen="2180000"
/vntifkey="29"
/label=CMV\promoter
rep_origin 1347..1775
/invitrogen="2500000"
/vntifkey="33"
/label=f1\origin
misc_feature 895..1059
/invitrogen="1630000"
/vntifkey="21"
/label=MCS
CDS 2185..2979
/invitrogen="1260000"
/vntifkey="4"
/label=Neo(R)
rep_origin complement(3666..4336)
/invitrogen="2540000"
/vntifkey="33"
/label=pUC\origin
promoter 1780..2149
/invitrogen="2290000"
/vntifkey="29"
/label=SV40\early\promoter
polyA_signal 3153..3283
/invitrogen="1840000"
/vntifkey="25"
/label=SV40\pA
primer 863..882
/invitrogen="2100000"
/vntifkey="27"
/label=T7\primer
promoter 863..879
/invitrogen="2370000"
/vntifkey="30"
/label=T7\promoter
misc_feature 935..1000
/vntifkey="21"
/label=P2A
BASE COUNT 1265 a 1424 c 1403 g 1385 t
ORIGIN
1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc
901 gtttaaactt aagcttggta ccgagctcgg atccggaagc ggagctacta acttcagcct
961 gctgaagcag gctggagacg tggaggagaa ccctggacct gaattctgca gatatccagc
1021 acagtggcgg ccgctcgagt ctagagggcc cgtttaaacc cgctgatcag cctcgactgt
1081 gccttctagt tgccagccat ctgttgtttg cccctccccc gtgccttcct tgaccctgga
1141 aggtgccact cccactgtcc tttcctaata aaatgaggaa attgcatcgc attgtctgag
1201 taggtgtcat tctattctgg ggggtggggt ggggcaggac agcaaggggg aggattggga
1261 agacaatagc aggcatgctg gggatgcggt gggctctatg gcttctgagg cggaaagaac
1321 cagctggggc tctagggggt atccccacgc gccctgtagc ggcgcattaa gcgcggcggg
1381 tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt
1441 cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg
1501 ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga
1561 ttagggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac
1621 gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc
1681 tatctcggtc tattcttttg atttataagg gattttgccg atttcggcct attggttaaa
1741 aaatgagctg atttaacaaa aatttaacgc gaattaattc tgtggaatgt gtgtcagtta
1801 gggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat
1861 tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc
1921 atgcatctca attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta
1981 actccgccca gttccgccca ttctccgccc catggctgac taattttttt tatttatgca
2041 gaggccgagg ccgcctctgc ctctgagcta ttccagaagt agtgaggagg cttttttgga
2101 ggcctaggct tttgcaaaaa gctcccggga gcttgtatat ccattttcgg atctgatcaa
2161 gagacaggat gaggatcgtt tcgcatgatt gaacaagatg gattgcacgc aggttctccg
2221 gccgcttggg tggagaggct attcggctat gactgggcac aacagacaat cggctgctct
2281 gatgccgccg tgttccggct gtcagcgcag gggcgcccgg ttctttttgt caagaccgac
2341 ctgtccggtg ccctgaatga actgcaggac gaggcagcgc ggctatcgtg gctggccacg
2401 acgggcgttc cttgcgcagc tgtgctcgac gttgtcactg aagcgggaag ggactggctg
2461 ctattgggcg aagtgccggg gcaggatctc ctgtcatctc accttgctcc tgccgagaaa
2521 gtatccatca tggctgatgc aatgcggcgg ctgcatacgc ttgatccggc tacctgccca
2581 ttcgaccacc aagcgaaaca tcgcatcgag cgagcacgta ctcggatgga agccggtctt
2641 gtcgatcagg atgatctgga cgaagagcat caggggctcg cgccagccga actgttcgcc
2701 aggctcaagg cgcgcatgcc cgacggcgag gatctcgtcg tgacccatgg cgatgcctgc
2761 ttgccgaata tcatggtgga aaatggccgc ttttctggat tcatcgactg tggccggctg
2821 ggtgtggcgg accgctatca ggacatagcg ttggctaccc gtgatattgc tgaagagctt
2881 ggcggcgaat gggctgaccg cttcctcgtg ctttacggta tcgccgctcc cgattcgcag
2941 cgcatcgcct tctatcgcct tcttgacgag ttcttctgag cgggactctg gggttcgaaa
3001 tgaccgacca agcgacgccc aacctgccat cacgagattt cgattccacc gccgccttct
3061 atgaaaggtt gggcttcgga atcgttttcc gggacgccgg ctggatgatc ctccagcgcg
3121 gggatctcat gctggagttc ttcgcccacc ccaacttgtt tattgcagct tataatggtt
3181 acaaataaag caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta
3241 gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctgtataccg tcgacctcta
3301 gctagagctt ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca
3361 caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag
3421 tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt
3481 cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc
3541 gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg
3601 tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa
3661 agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg
3721 cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga
3781 ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg
3841 tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg
3901 gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc
3961 gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg
4021 gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca
4081 ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt
4141 ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag
4201 ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg
4261 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt
4321 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg
4381 tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta
4441 aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg
4501 aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg
4561 tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc
4621 gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg
4681 agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg
4741 aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag
4801 gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat
4861 caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc
4921 cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc
4981 ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa
5041 ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac
5101 gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt
5161 cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc
5221 gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa
5281 caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca
5341 tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat
5401 acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa
5461 aagtgccacc tgacgtc
//
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