pLVX-CherryPicker1

价格:3500元

联系方式:I47-825O-882O

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载体基本信息

出品公司: Clontech
载体名称: pLVX-CherryPicker1
质粒类型: 慢病毒载体;荧光报告载体
高拷贝/低拷贝: 高拷贝
克隆方法: 限制性内切酶,多克隆位点
启动子: CMV
载体大小: 8349 bp
5' 测序引物及序列: CMV Forward: CGCAAATGGGCGGTAGGCGTG
3' 测序引物及序列: --
载体标签: CherryPicker1(C-端)
载体抗性: 氨苄青霉素
筛选标记: 嘌呤霉素(Puromycin)
克隆菌株: Stbl3
宿主细胞(系): 常规细胞系(293、CV-1、CHO等)
备注: 慢病毒载体pLVX-CherryPicker1组成型表达CherryPicker融合蛋白;
CherryPicker是mCherry与转铁蛋白受体膜锚定结构域融合蛋白。
产品目录号: 632580
稳定性: 稳表达
组成型/诱导型: 组成型
病毒/非病毒: 慢病毒

载体质粒图谱和多克隆位点信息

pLVX-CherryPicker1载体图谱



pLVX-CherryPicker1 多克隆位点

pLVX-CherryPicker1 载体特征

载体简介

载体序列

LOCUS       pLVX-CherryPicker1   8349 bp    DNA   circular             8-OCT-2010
COMMENT     Created by Clontech Laboratories Inc. http://www.clontech.com
COMMENT     Cat. No. 632580,632574
FEATURES             Location/Qualifiers
     LTR             1..635
                     /label=5'\LTR
     misc_feature    685..822
                     /label=packaging\signal
     misc_feature    2028..2151
                     /label=cPPT/CTS\(DNA\flap)
     misc_feature    4318..4909
                     /label=WPRE
     rep_origin      complement(6217..6890)
                     /label=pUC\origin
     CDS             complement(7035..8031)
                     /label=Amp(R)
     misc_binding    1303..1536
                     /label=RRE
     misc_binding    636..653
                     /label=PBS
     LTR             5112..5748
                     /label=3'\LTR
     misc_feature    3176..3684
                     /label=PGK\promoter
     misc_feature    3705..4304
                     /label=Puro(R)
     CDS             2236..3162
                     /label=CherryPicker
     misc_feature    3163..3165
                     /label=Stop\codon
     misc_feature    2206..2235
                     /label=MCS
BASE COUNT     2105 a      2140 c      2248 g      1856 t 
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca 
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac 
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca 
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg 
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag 
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg 
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat 
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga 
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct 
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc 
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag 
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg 
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga 
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg 
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg 
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct 
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat 
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca 
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc 
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag 
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc 
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg 
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc 
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc 
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct 
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa 
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca 
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt 
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt 
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta 
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt 
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct 
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga 
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag 
     2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac 
     2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg 
     2161 acagcagaga tccagtttat cgaattctgc agtcgacggt accgcgggcc cgggatccgc 
     2221 ggccgcgttt aaacgatgat ggtggacggc gacaacagcc acgtggagat gaagctggcc 
     2281 gtggacgagg aagagaacgc cgacaacaac accaaggcca acgtgaccaa gcccaagcgg 
     2341 tgcagcggca gcatctgcta cggcacaatc gccgtgatcg tgttcttcct gatcggcttc 
     2401 atgatcggct acctgggcta ctgcaagtct agcgacggcc ctggcgagac aggcatggtg 
     2461 tccaagggcg aagaggacaa catggccatc atcaaagaat tcatgcggtt caaggtgcac 
     2521 atggaaggca gcgtgaacgg ccacgagttc gagatcgagg gcgagggcga aggcagacct 
     2581 tacgagggca cccagaccgc caagctgaaa gtgaccaagg gcggacccct gccctttgcc 
     2641 tgggacatcc tgagccccca gttcatgtac ggcagcaagg cctacgtgaa gcaccccgcc 
     2701 gacatccccg actacctgaa gctgagcttc cccgagggct tcaagtggga gagagtgatg 
     2761 aacttcgagg acggcggcgt ggtcaccgtg acccaggaca gcagcctgca ggacggcgag 
     2821 ttcatctaca aagtgaagct gcggggcacc aacttcccta gcgacggccc cgtgatgcag 
     2881 aaaaagacca tgggctggga ggccagcagc gagagaatgt accccgagga tggcgccctg 
     2941 aagggcgaga tcaagcagcg gctgaagctg aaggatggcg gccactacga cgccgaagtg 
     3001 aaaaccacct acaaggccaa gaaacccgtg cagctgcctg gcgcctacaa cgtgaacatc 
     3061 aagctggaca tcaccagcca caacgaggac tacaccatcg tggagcagta cgagagagcc 
     3121 gagggcagac acagcaccgg cggcatggac gagctgtaca aatgacaatc tagataattc 
     3181 taccgggtag gggaggcgct tttcccaagg cagtctggag catgcgcttt agcagccccg 
     3241 ctgggcactt ggcgctacac aagtggcctc tggcctcgca cacattccac atccaccggt 
     3301 aggcgccaac cggctccgtt ctttggtggc cccttcgcgc caccttctac tcctccccta 
     3361 gtcaggaagt tcccccccgc cccgcagctc gcgtcgtgca ggacgtgaca aatggaagta 
     3421 gcacgtctca ctagtctcgt gcagatggac agcaccgctg agcaatggaa gcgggtaggc 
     3481 ctttggggca gcggccaata gcagctttgc tccttcgctt tctgggctca gaggctggga 
     3541 aggggtgggt ccgggggcgg gctcaggggc gggctcaggg gcggggcggg cgcccgaagg 
     3601 tcctccggag gcccggcatt ctgcacgctt caaaagcgca cgtctgccgc gctgttctcc 
     3661 tcttcctcat ctccgggcct ttcgacctgc agcccaagct taccatgacc gagtacaagc 
     3721 ccacggtgcg cctcgccacc cgcgacgacg tccccagggc cgtacgcacc ctcgccgccg 
     3781 cgttcgccga ctaccccgcc acgcgccaca ccgtcgatcc ggaccgccac atcgagcggg 
     3841 tcaccgagct gcaagaactc ttcctcacgc gcgtcgggct cgacatcggc aaggtgtggg 
     3901 tcgcggacga cggcgccgcg gtggcggtct ggaccacgcc ggagagcgtc gaagcggggg 
     3961 cggtgttcgc cgagatcggc ccgcgcatgg ccgagttgag cggttcccgg ctggccgcgc 
     4021 agcaacagat ggaaggcctc ctggcgccgc accggcccaa ggagcccgcg tggttcctgg 
     4081 ccaccgtcgg cgtctcgccc gaccaccagg gcaagggtct gggcagcgcc gtcgtgctcc 
     4141 ccggagtgga ggcggccgag cgcgccgggg tgcccgcctt cctggagacc tccgcgcccc 
     4201 gcaacctccc cttctacgag cggctcggct tcaccgtcac cgccgacgtc gaggtgcccg 
     4261 aaggaccgcg cacctggtgc atgacccgca agcccggtgc ctgaccgcgt ctggaacaat 
     4321 caacctctgg attacaaaat ttgtgaaaga ttgactggta ttcttaacta tgttgctcct 
     4381 tttacgctat gtggatacgc tgctttaatg cctttgtatc atgctattgc ttcccgtatg 
     4441 gctttcattt tctcctcctt gtataaatcc tggttgctgt ctctttatga ggagttgtgg 
     4501 cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt 
     4561 tggggcattg ccaccacctg tcagctcctt tccgggactt tcgctttccc cctccctatt 
     4621 gccacggcgg aactcatcgc cgcctgcctt gcccgctgct ggacaggggc tcggctgttg 
     4681 ggcactgaca attccgtggt gttgtcgggg aagctgacgt cctttccatg gctgctcgcc 
     4741 tgtgttgcca cctggattct gcgcgggacg tccttctgct acgtcccttc ggccctcaat 
     4801 ccagcggacc ttccttcccg cggcctgctg ccggctctgc ggcctcttcc gcgtcttcgc 
     4861 cttcgccctc agacgagtcg gatctccctt tgggccgcct ccccgcctgg aattaattct 
     4921 gcagtcgaga cctagaaaaa catggagcaa tcacaagtag caatacagca gctaccaatg 
     4981 ctgattgtgc ctggctagaa gcacaagagg aggaggaggt gggttttcca gtcacacctc 
     5041 aggtaccttt aagaccaatg acttacaagg cagctgtaga tcttagccac tttttaaaag 
     5101 aaaagagggg actggaaggg ctaattcact cccaacgaag acaagatatc cttgatctgt 
     5161 ggatctacca cacacaaggc tacttccctg attagcagaa ctacacacca gggccagggg 
     5221 tcagatatcc actgaccttt ggatggtgct acaagctagt accagttgag ccagataagg 
     5281 tagaagaggc caataaagga gagaacacca gcttgttaca ccctgtgagc ctgcatggga 
     5341 tggatgaccc ggagagagaa gtgttagagt ggaggtttga cagccgccta gcatttcatc 
     5401 acgtggcccg agagctgcat ccggagtact tcaagaactg ctgatatcga gcttgctaca 
     5461 agggactttc cgctggggac tttccaggga ggcgtggcct gggcgggact ggggagtggc 
     5521 gagccctcag atcctgcata taagcagctg ctttttgcct gtactgggtc tctctggtta 
     5581 gaccagatct gagcctggga gctctctggc taactaggga acccactgct taagcctcaa 
     5641 taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac 
     5701 tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtag tagttcatgt 
     5761 catcttatta ttcagtattt ataacttgca aagaaatgaa tatcagagag tgagaggcct 
     5821 tgacattgct agcgtttacc gtcgacctct agctagagct tggcgtaatc atggtcatag 
     5881 ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc 
     5941 ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc 
     6001 tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa 
     6061 cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg 
     6121 ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg 
     6181 ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag 
     6241 gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac 
     6301 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga 
     6361 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt 
     6421 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc 
     6481 tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc 
     6541 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta 
     6601 agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat 
     6661 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca 
     6721 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct 
     6781 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt 
     6841 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct 
     6901 cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc 
     6961 acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa 
     7021 acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta 
     7081 tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc 
     7141 ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat 
     7201 ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta 
     7261 tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt 
     7321 aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt 
     7381 ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg 
     7441 ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc 
     7501 gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc 
     7561 gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg 
     7621 cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga 
     7681 actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta 
     7741 ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct 
     7801 tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag 
     7861 ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca atattattga 
     7921 agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat 
     7981 aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt cgacggatcg 
     8041 ggagatcaac ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa 
     8101 tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa 
     8161 tgtatcttat catgtctgga tcaactggat aactcaagct aaccaaaatc atcccaaact 
     8221 tcccacccca taccctatta ccactgccaa ttacctgtgg tttcatttac tctaaacctg 
     8281 tgattcctct gaattatttt cattttaaag aaattgtatt tgttaaatat gtactacaaa 
     8341 cttagtagt 
//


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