plenti-UBC-gate-3xHA-pGK-PUR

LOCUS       plenti-UBC-gate-3xHA-pGK-PUR               11227 bp ds-DNA     circular SYN 12-MAY-2021
DEFINITION  Lentiviral gateway destination vector .
ACCESSION   .
VERSION     .
KEYWORDS    plenti-UBC-gate-3xHA-pGK-PUR
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11227)
  AUTHORS   Lu G, Middleton RE, Sun H, Naniong M, Ott CJ, Mitsiades CS, Wong KK,
            Bradner JE, Kaelin WG Jr
  TITLE     The myeloma drug lenalidomide promotes the cereblon-dependent 
            destruction of Ikaros proteins.
  JOURNAL   Science. 2014 Jan 17;343(6168):305-9. doi: 10.1126/science.1244917. 
            Epub 2013 Nov 29.
  PUBMED    24292623
REFERENCE   2  (bases 1 to 11227)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 12, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..11227
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        76..1287
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     1124..1143
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     protein_bind    1335..1459
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        1484..1514
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             1568..2227
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(1634..1653)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             2569..2874
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     2753..2772
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(2915..3039)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             3086..3175
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAGYPYDVPDYAGSYPYDVPDYA"
     promoter        3217..3716
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(3252..3275)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     3649..3668
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             3737..4336
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(3737..3756)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     4233..4253
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     protein_bind    4367..4400
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
     misc_feature    4456..5044
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4509..4529)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4927..4938)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(5047..5063)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5048..5064)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             5253..5486
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    5558..5679
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5595..5614)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5649..5668
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      5719..5854
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(5874..5893)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5875..5893)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      complement(5916..6274)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5956..5977)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     6168..6187
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        6301..6405
                     /gene="bla"
                     /label=AmpR promoter
     promoter        6407..6764
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     primer_bind     complement(6430..6450)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      6615..6750
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             6799..7593
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6853..6872)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     7463..7482
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(7781..7800)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    7825..7872
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      8201..8789
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8690..8709
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     enhancer        8964..9267
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        9269..9467
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     9422..9442
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             9485..9665
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    9712..9837
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    10334..10567
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             10752..10796
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    11094..11211
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 acagcagaga tccagtttgg ttagtaccgg gcccgctcta gccctagatt taacccaaca
       61 gaaggctcga cgcgtggcct ccgcgccggg ttttggcgcc tcccgcgggc gcccccctcc
      121 tcacggcgag cgctgccacg tcagacgaag ggcgcagcga gcgtcctgat ccttccgccc
      181 ggacgctcag gacagcggcc cgctgctcat aagactcggc cttagaaccc cagtatcagc
      241 agaaggacat tttaggacgg gacttgggtg actctagggc actggttttc tttccagaga
      301 gcggaacagg cgaggaaaag tagtcccttc tcggcgattc tgcggaggga tctccgtggg
      361 gcggtgaacg ccgatgatta tataaggacg cgccgggtgt ggcacagcta gttccgtcgc
      421 agccgggatt tgggtcgcgg ttcttgtttg tggatcgctg tgatcgtcac ttggtgagta
      481 gcgggctgct gggctggccg gggctttcgt ggccgccggg ccgctcggtg ggacggaagc
      541 gtgtggagag accgccaagg gctgtagtct gggtccgcga gcaaggttgc cctgaactgg
      601 gggttggggg gagcgcagca aaatggcggc tgttcccgag tcttgaatgg aagacgcttg
      661 tgaggcgggc tgtgaggtcg ttgaaacaag gtggggggca tggtgggcgg caagaaccca
      721 aggtcttgag gccttcgcta atgcgggaaa gctcttattc gggtgagatg ggctggggca
      781 ccatctgggg accctgacgt gaagtttgtc actgactgga gaactcggtt tgtcgtctgt
      841 tgcgggggcg gcagttatgg cggtgccgtt gggcagtgca cccgtacctt tgggagcgcg
      901 cgccctcgtc gtgtcgtgac gtcacccgtt ctgttggctt ataatgcagg gtggggccac
      961 ctgccggtag gtgtgcggta ggcttttctc cgtcgcagga cgcagggttc gggcctaggg
     1021 taggctctcc tgaatcgaca ggcgccggac ctctggtgag gggagggata agtgaggcgt
     1081 cagtttcttt ggtcggtttt atgtacctat cttcttaagt agctgaagct ccggttttga
     1141 actatgcgct cggggttggc gagtgtgttt tgtgaagttt tttaggcacc ttttgaaatg
     1201 taatcatttg ggtcaatatg taattttcag tgttagacta gtaaattgtc cgctaaattc
     1261 tggccgtttt tggctttttt gttagacgct agactgccgg atcgaattgt cgacgaattc
     1321 ggatctggcc aatcacaagt ttgtacaaaa aagctgaacg agaaacgtaa aatgatataa
     1381 atatcaatat attaaattag attttgcata aaaaacagac tacataatac tgtaaaacac
     1441 aacatatcca gtcactatgg cggccgcatt aggcacccca ggctttacac tttatgcttc
     1501 cggctcgtat aatgtgtgga ttttgagtta ggatccgtcg agattttcag gagctaagga
     1561 agctaaaatg gagaaaaaaa tcactggata taccaccgtt gatatatccc aatggcatcg
     1621 taaagaacat tttgaggcat ttcagtcagt tgctcaatgt acctataacc agaccgttca
     1681 gctggatatt acggcctttt taaagaccgt aaagaaaaat aagcacaagt tttatccggc
     1741 ctttattcac attcttgccc gcctgatgaa tgctcatccg gaattccgta tggcaatgaa
     1801 agacggtgag ctggtgatat gggatagtgt tcacccttgt tacaccgttt tccatgagca
     1861 aactgaaacg ttttcatcgc tctggagtga ataccacgac gatttccggc agtttctaca
     1921 catatattcg caagatgtgg cgtgttacgg tgaaaacctg gcctatttcc ctaaagggtt
     1981 tattgagaat atgtttttcg tctcagccaa tccctgggtg agtttcacca gttttgattt
     2041 aaacgtggcc aatatggaca acttcttcgc ccccgttttc accatgggca aatattatac
     2101 gcaaggcgac aaggtgctga tgccgctggc gattcaggtt catcatgccg tttgtgatgg
     2161 cttccatgtc ggcagaatgc ttaatgaatt acaacagtac tgcgatgagt ggcagggcgg
     2221 ggcgtaaacg cgtggatccg gcttactaaa agccagataa cagtatgcgt atttgcgcgc
     2281 tgatttttgc ggtataagaa tatatactga tatgtatacc cgaagtatgt caaaaagagg
     2341 tatgctatga agcagcgtat tacagtgaca gttgacagcg acagctatca gttgctcaag
     2401 gcatatatga tgtcaatatc tccggtctgg taagcacaac catgcagaat gaagcccgtc
     2461 gtctgcgtgc cgaacgctgg aaagcggaaa atcaggaagg gatggctgag gtcgcccggt
     2521 ttattgaaat gaacggctct tttgctgacg agaacagggg ctggtgaaat gcagtttaag
     2581 gtttacacct ataaaagaga gagccgttat cgtctgtttg tggatgtaca gagtgatatt
     2641 attgacacgc ccgggcgacg gatggtgatc cccctggcca gtgcacgtct gctgtcagat
     2701 aaagtctccc gtgaacttta cccggtggtg catatcgggg atgaaagctg gcgcatgatg
     2761 accaccgata tggccagtgt gccggtctcc gttatcgggg aagaagtggc tgatctcagc
     2821 caccgcgaaa atgacatcaa aaacgccatt aacctgatgt tctggggaat ataaatgtca
     2881 ggctccctta tacacagcca gtctgcaggt cgaccatagt gactggatat gttgtgtttt
     2941 acagtattat gtagtctgtt ttttatgcaa aatctaattt aatatattga tatttatatc
     3001 attttacgtt tctcgttcag ctttcttgta caaagtggtg ggtggaggcg gttcaggtgg
     3061 cggaggaagc ggaggcggtg gaagttaccc ctacgacgtg cccgactacg ccggctatcc
     3121 gtatgatgtc ccggactatg caggatccta tccatacgac gttccagatt acgcttagtt
     3181 aattaaggcc ggccctcgag gttaacgaat tctaccgggt aggggaggcg cttttcccaa
     3241 ggcagtctgg agcatgcgct ttagcagccc cgctgggcac ttggcgctac acaagtggcc
     3301 tctggcctcg cacacattcc acatccaccg gtaggcgcca accggctccg ttctttggtg
     3361 gccccttcgc gccaccttct actcctcccc tagtcaggaa gttccccccc gccccgcagc
     3421 tcgcgtcgtg caggacgtga caaatggaag tagcacgtct cactagtctc gtgcagatgg
     3481 acagcaccgc tgagcaatgg aagcgggtag gcctttgggg cagcggccaa tagcagcttt
     3541 gctccttcgc tttctgggct cagaggctgg gaaggggtgg gtccgggggc gggctcaggg
     3601 gcgggctcag gggcggggcg ggcgcccgaa ggtcctccgg aggcccggca ttctgcacgc
     3661 ttcaaaagcg cacgtctgcc gcgctgttct cctcttcctc atctccgggc ctttcgacct
     3721 gcagcccaag cttaccatga ccgagtacaa gcccacggtg cgcctcgcca cccgcgacga
     3781 cgtccccagg gccgtacgca ccctcgccgc cgcgttcgcc gactaccccg ccacgcgcca
     3841 caccgtcgat ccggaccgcc acatcgagcg ggtcaccgag ctgcaagaac tcttcctcac
     3901 gcgcgtcggg ctcgacatcg gcaaggtgtg ggtcgcggac gacggcgccg cggtggcggt
     3961 ctggaccacg ccggagagcg tcgaagcggg ggcggtgttc gccgagatcg gcccgcgcat
     4021 ggccgagttg agcggttccc ggctggccgc gcagcaacag atggaaggcc tcctggcgcc
     4081 gcaccggccc aaggagcccg cgtggttcct ggccaccgtc ggcgtctcgc ccgaccacca
     4141 gggcaagggt ctgggcagcg ccgtcgtgct ccccggagtg gaggcggccg agcgcgccgg
     4201 ggtgcccgcc ttcctggaga cctccgcgcc ccgcaacctc cccttctacg agcggctcgg
     4261 cttcaccgtc accgccgacg tcgaggtgcc cgaaggaccg cgcacctggt gcatgacccg
     4321 caagcccggt gcctgatgta caagtaggaa ttcgtcgagg gacctaataa cttcgtatag
     4381 catacattat acgaagttat acatgtttaa gggttccggt tccactaggt acaattcgat
     4441 atcaagctta tcgataatca acctctggat tacaaaattt gtgaaagatt gactggtatt
     4501 cttaactatg ttgctccttt tacgctatgt ggatacgctg ctttaatgcc tttgtatcat
     4561 gctattgctt cccgtatggc tttcattttc tcctccttgt ataaatcctg gttgctgtct
     4621 ctttatgagg agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct
     4681 gacgcaaccc ccactggttg gggcattgcc accacctgtc agctcctttc cgggactttc
     4741 gctttccccc tccctattgc cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg
     4801 acaggggctc ggctgttggg cactgacaat tccgtggtgt tgtcggggaa atcatcgtcc
     4861 tttccttggc tgctcgcctg tgttgccacc tggattctgc gcgggacgtc cttctgctac
     4921 gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg
     4981 cctcttccgc gtcttcgcct tcgccctcag acgagtcgga tctccctttg ggccgcctcc
     5041 ccgcatcgat accgtcgacc tcgatcgaga cctagaaaaa catggagcaa tcacaagtag
     5101 caatacagca gctaccaatg ctgattgtgc ctggctagaa gcacaagagg aggaggaggt
     5161 gggttttcca gtcacacctc aggtaccttt aagaccaatg acttacaagg cagctgtaga
     5221 tcttagccac tttttaaaag aaaagggggg actggaaggg ctaattcact cccaacgaag
     5281 acaagatctg ctttttgctt gtactgggtc tctctggtta gaccagatct gagcctggga
     5341 gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct
     5401 tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt
     5461 ttagtcagtg tggaaaatct ctagcagtag tagttcatgt catcttatta ttcagtattt
     5521 ataacttgca aagaaatgaa tatcagagag tgagaggaac ttgtttattg cagcttataa
     5581 tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca
     5641 ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctggc tctagctatc
     5701 ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc
     5761 catggctgac taattttttt tatttatgca gaggccgagg ccgcctcggc ctctgagcta
     5821 ttccagaagt agtgaggagg cttttttgga ggcctaggga cgtacccaat tcgccctata
     5881 gtgagtcgta ttagcatgtg agcaaaaggc cagcataaat caaaagaata gaccgagata
     5941 gggttgagtg ttgttccagt ttggaacaag agtccactat taaagaacgt ggactccaac
     6001 gtcaaagggc gaaaaaccgt ctatcagggc gatggcccac tacgtgaacc atcaccctaa
     6061 tcaagttttt tggggtcgag gtgccgtaaa gcactaaatc ggaaccctaa agggagcccc
     6121 cgatttagag cttgacgggg aaagccggcg aacgtggcga gaaaggaagg gaagaaagcg
     6181 aaaggagcgg gcgctagggc gctggcaagt gtagcggtca cgctgcgcgt aaccaccaca
     6241 cccgccgcgc ttaatgcgcc gctacagggc gcgtcaggtg gcacttttcg gggaaatgtg
     6301 cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga
     6361 caataaccct gataaatgct tcaataatat tgaaaaagga agagtcctga ggcggaaaga
     6421 accagctgtg gaatgtgtgt cagttagggt gtggaaagtc cccaggctcc ccagcaggca
     6481 gaagtatgca aagcatgcat ctcaattagt cagcaaccag gtgtggaaag tccccaggct
     6541 ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc atagtcccgc
     6601 ccctaactcc gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg
     6661 gctgactaat tttttttatt tatgcagagg ccgaggccgc ctcggcctct gagctattcc
     6721 agaagtagtg aggaggcttt tttggaggcc taggcttttg caaagatcga tcaagagaca
     6781 ggatgaggat cgtttcgcat gattgaacaa gatggattgc acgcaggttc tccggccgct
     6841 tgggtggaga ggctattcgg ctatgactgg gcacaacaga caatcggctg ctctgatgcc
     6901 gccgtgttcc ggctgtcagc gcaggggcgc ccggttcttt ttgtcaagac cgacctgtcc
     6961 ggtgccctga atgaactgca agacgaggca gcgcggctat cgtggctggc cacgacgggc
     7021 gttccttgcg cagctgtgct cgacgttgtc actgaagcgg gaagggactg gctgctattg
     7081 ggcgaagtgc cggggcagga tctcctgtca tctcaccttg ctcctgccga gaaagtatcc
     7141 atcatggctg atgcaatgcg gcggctgcat acgcttgatc cggctacctg cccattcgac
     7201 caccaagcga aacatcgcat cgagcgagca cgtactcgga tggaagccgg tcttgtcgat
     7261 caggatgatc tggacgaaga gcatcagggg ctcgcgccag ccgaactgtt cgccaggctc
     7321 aaggcgagca tgcccgacgg cgaggatctc gtcgtgaccc atggcgatgc ctgcttgccg
     7381 aatatcatgg tggaaaatgg ccgcttttct ggattcatcg actgtggccg gctgggtgtg
     7441 gcggaccgct atcaggacat agcgttggct acccgtgata ttgctgaaga gcttggcggc
     7501 gaatgggctg accgcttcct cgtgctttac ggtatcgccg ctcccgattc gcagcgcatc
     7561 gccttctatc gccttcttga cgagttcttc tgagcgggac tctggggttc gaaatgaccg
     7621 accaagcgac gcccaacctg ccatcacgag atttcgattc caccgccgcc ttctatgaaa
     7681 ggttgggctt cggaatcgtt ttccgggacg ccggctggat gatcctccag cgcggggatc
     7741 tcatgctgga gttcttcgcc caccctaggg ggaggctaac tgaaacacgg aaggagacaa
     7801 taccggaagg aacccgcgct atgacggcaa taaaaagaca gaataaaacg cacggtgttg
     7861 ggtcgtttgt tcataaacgc ggggttcggt cccagggctg gcactctgtc gataccccac
     7921 cgagacccca ttggggccaa tacgcccgcg tttcttcctt ttccccaccc caccccccaa
     7981 gttcgggtga aggcccaggg ctcgcagcca acgtcggggc ggcaggccct gccatagcct
     8041 caggttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct
     8101 aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc
     8161 actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc
     8221 gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg
     8281 atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa
     8341 atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc
     8401 ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt
     8461 gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa
     8521 cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc
     8581 tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc
     8641 cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct
     8701 ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat
     8761 gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc
     8821 tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg
     8881 ataaccgtat taccgccatg cattagttat taatagtaat caattacggg gtcattagtt
     8941 catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga
     9001 ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca
     9061 atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca
     9121 gtacatcaag tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg
     9181 cccgcctggc attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc
     9241 tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt
     9301 ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt
     9361 ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg
     9421 acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagcgc gttttgcctg
     9481 tactgggtct ctctggttag accagatctg agcctgggag ctctctggct aactagggaa
     9541 cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct
     9601 gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc
     9661 tagcagtggc gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga
     9721 cgcaggactc ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt
     9781 acgccaaaaa ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt
     9841 attaagcggg ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag
     9901 aaaaaatata aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt
     9961 aatcctggcc tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca
    10021 tcccttcaga caggatcaga agaacttaga tcattatata atacagtagc aaccctctat
    10081 tgtgtgcatc aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa
    10141 gagcaaaaca aaagtaagac caccgcacag caagcggccg gccgctgatc ttcagacctg
    10201 gaggaggaga tatgagggac aattggagaa gtgaattata taaatataaa gtagtaaaaa
    10261 ttgaaccatt aggagtagca cccaccaagg caaagagaag agtggtgcag agagaaaaaa
    10321 gagcagtggg aataggagct ttgttccttg ggttcttggg agcagcagga agcactatgg
    10381 gcgcagcgtc aatgacgctg acggtacagg ccagacaatt attgtctggt atagtgcagc
    10441 agcagaacaa tttgctgagg gctattgagg cgcaacagca tctgttgcaa ctcacagtct
    10501 ggggcatcaa gcagctccag gcaagaatcc tggctgtgga aagataccta aaggatcaac
    10561 agctcctggg gatttggggt tgctctggaa aactcatttg caccactgct gtgccttgga
    10621 atgctagttg gagtaataaa tctctggaac agatttggaa tcacacgacc tggatggagt
    10681 gggacagaga aattaacaat tacacaagct taatacactc cttaattgaa gaatcgcaaa
    10741 accagcaaga aaagaatgaa caagaattat tggaattaga taaatgggca agtttgtgga
    10801 attggtttaa cataacaaat tggctgtggt atataaaatt attcataatg atagtaggag
    10861 gcttggtagg tttaagaata gtttttgctg tactttctat agtgaataga gttaggcagg
    10921 gatattcacc attatcgttt cagacccacc tcccaacccc gaggggaccc gacaggcccg
    10981 aaggaataga agaagaaggt ggagagagag acagagacag atccattcga ttagtgaacg
    11041 gatcggcact gcgtgcgcca attctgcaga caaatggcag tattcatcca caattttaaa
    11101 agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat aatagcaaca
    11161 gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt tcgggtttat
    11221 tacaggg
//