| 质粒名称: | pcDNA3-cmyc |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 16011 |
| 存储实验室: | Wafik El-Deiry |
|
载体骨架基本信息 |
|
| 载体名称: | pcDNA3 |
| 载体出品公司: | Invitrogen |
| 载体抗性: | 氨苄青霉素 |
| 空载体大小: | 5400 bp |
| 完整质粒大小: | -- |
| 载体修饰: | -- |
| 载体类型: | 哺乳细胞表达 |
| 筛选标记: | 新霉素（G418） |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | cmyc |
| 物种来源: | H. sapiens (human) |
| 插入大小: | -- |
| 基因突变: | -- |
| GenBank编号 | NM_002467.2 NP_002458.1 |
| 启动子 | -- |
| 标签/融合蛋白: | -- |
| 克隆方法: | 限制性内切酶 |
| 5' 克隆位点: | -- |
| 3' 克隆位点: | -- |
| 5' 测序引物 | CMV-F |
| 3' 测序引物 | -- |
|
文献引用方法 |
|
| 材料和方法部分: | pcDNA3-cmyc was a gift from Wafik El-Deiry (Addgene plasmid # 16011 ; http://n2t.net/addgene:16011 ; RRID:Addgene_16011) |
| 参考文献部分: | Direct repression of FLIP expression by c-myc is a major determinant of TRAIL sensitivity. Ricci MS, Jin Z, Dews M, Yu D, Thomas-Tikhonenko A, Dicker DT, El-Deiry WS. Mol Cell Biol. 2004 Oct . 24(19):8541-55. 10.1128/MCB.24.19.8541-8555.2004 PubMed 15367674 |
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pcDNA3-cmyc载体 pcDNA3cmyc质粒基本信息
pcDNA3-cmyc质粒图谱 pcDNA3cmyc载体图谱和pcDNA3-cmyc载体序列 pcDNA3cmyc质粒序列多克隆位点信息
pcDNA3-cmyc质粒 pcDNA3cmyc载体简介
pcDNA3-cmyc质粒序列 pcDNA3cmyc载体序列
LOCUS pcDNA3-cmyc 6808 bp ds-DNA circular SYN 06-AUG-2018
DEFINITION mammalian expression of human cmyc.
ACCESSION .
VERSION .
KEYWORDS pcDNA3-cmyc
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6808)
AUTHORS Ricci MS, Jin Z, Dews M, Yu D, Thomas-Tikhonenko A, Dicker DT,
El-Deiry WS
TITLE Direct repression of FLIP expression by c-myc is a major determinant
of TRAIL sensitivity.
JOURNAL Mol Cell Biol. 2004 Oct . 24(19):8541-55.
PUBMED 15367674
REFERENCE 2 (bases 1 to 6808)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Aug 6, 2018 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..6808
/organism="synthetic DNA construct"
/mol_type="other DNA"
rep_origin 132..267
/label=SV40 ori
/note="SV40 origin of replication"
primer_bind 194..213
/label=SV40pro-F
/note="SV40 promoter/origin, forward primer"
CDS 348..1142
/codon_start=1
/gene="aph(3')-II (or nptII)"
/product="aminoglycoside phosphotransferase from Tn5"
/label=NeoR/KanR
/note="confers resistance to neomycin, kanamycin, and G418
(Geneticin(R))"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
primer_bind complement(402..421)
/label=Neo-R
/note="Neomycin resistance gene, reverse primer"
primer_bind 1012..1031
/label=Neo-F
/note="Neomycin resistance gene, forward primer"
polyA_signal 1316..1437
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(1353..1372)
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind 1407..1426
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
primer_bind complement(1486..1502)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(1486..1502)
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind complement(1499..1521)
/label=M13/pUC Reverse
/note="In lacZ gene"
protein_bind 1510..1526
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(1534..1564)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 1579..1600
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
primer_bind complement(1717..1734)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(1888..2476)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(1968..1987)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(2647..3507)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 3270..3289
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(3508..3612)
/gene="bla"
/label=AmpR promoter
primer_bind complement(3687..3706)
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
enhancer 3878..4257
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 4258..4461
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind 4412..4432
/label=CMV-F
/note="Human CMV immediate early promoter, forward primer"
primer_bind 4506..4525
/label=T7
/note="T7 promoter, forward primer"
promoter 4506..4524
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
CDS 4607..5926
/codon_start=1
/product="human c-Myc proto-oncogene"
/label=c-Myc
/translation="MPLNVSFTNRNYDLDYDSVQPYFYCDEEENFYQQQQQSELQPPAP
SEDIWKKFELLPTPPLSPSRRSGLCSPSYVAVTPFSLRGDNDGGGGSFSTADQLEMVTE
LLGGDMVNQSFICDPDDETFIKNIIIQDCMWSGFSAAAKLVSEKLASYQAARKDSGSPN
PARGHSVCSTSSLYLQDLSAAASECIDPSVVFPYPLNDSSSPKSCASQDSSAFSPSSDS
LLSSTESSPQGSPEPLVLHEETPPTTSSDSEEEQEDEEEIDVVSVEKRQAPGKRSESGS
PSAGGHSKPPHSPLVLKRCHVSTHQHNYAAPPSTRKDYPAAKRVKLDSVRVLRQISNNR
KCTSPRSSDTEENVKRRTHNVLERQRRNELKRSFFALRDQIPELENNEKAPKVVILKKA
TAYILSVQAEEQKLISEEDLLRKRREQLKHKLEQLRNSCA"
promoter complement(6004..6022)
/label=SP6 promoter
/note="promoter for bacteriophage SP6 RNA polymerase"
primer_bind complement(6005..6022)
/label=SP6
/note="SP6 promoter, forward primer"
primer_bind complement(6042..6059)
/label=BGH-rev
/note="Bovine growth hormone terminator, reverse primer.
Also called BGH reverse"
polyA_signal 6048..6272
/label=bGH poly(A) signal
/note="bovine growth hormone polyadenylation signal"
rep_origin 6318..6746
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(6405..6424)
/label=F1ori-R
/note="F1 origin, reverse primer"
primer_bind 6615..6636
/label=F1ori-F
/note="F1 origin, forward primer"
primer_bind complement(6755..6775)
/label=pBABE 3'
/note="SV40 enhancer, reverse primer for pBABE vectors"
promoter 6760..281
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
ORIGIN
1 gtatgcaaag catgcatctc aattagtcag caaccaggtg tggaaagtcc ccaggctccc
61 cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccata gtcccgcccc
121 taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct
181 gactaatttt ttttatttat gcagaggccg aggccgcctc tgcctctgag ctattccaga
241 agtagtgagg aggctttttt ggaggcctag gcttttgcaa aaagctcccg ggagcttgta
301 tatccatttt cggatctgat caagagacag gatgaggatc gtttcgcatg attgaacaag
361 atggattgca cgcaggttct ccggccgctt gggtggagag gctattcggc tatgactggg
421 cacaacagac aatcggctgc tctgatgccg ccgtgttccg gctgtcagcg caggggcgcc
481 cggttctttt tgtcaagacc gacctgtccg gtgccctgaa tgaactgcag gacgaggcag
541 cgcggctatc gtggctggcc acgacgggcg ttccttgcgc agctgtgctc gacgttgtca
601 ctgaagcggg aagggactgg ctgctattgg gcgaagtgcc ggggcaggat ctcctgtcat
661 ctcaccttgc tcctgccgag aaagtatcca tcatggctga tgcaatgcgg cggctgcata
721 cgcttgatcc ggctacctgc ccattcgacc accaagcgaa acatcgcatc gagcgagcac
781 gtactcggat ggaagccggt cttgtcgatc aggatgatct ggacgaagag catcaggggc
841 tcgcgccagc cgaactgttc gccaggctca aggcgcgcat gcccgacggc gaggatctcg
901 tcgtgaccca tggcgatgcc tgcttgccga atatcatggt ggaaaatggc cgcttttctg
961 gattcatcga ctgtggccgg ctgggtgtgg cggaccgcta tcaggacata gcgttggcta
1021 cccgtgatat tgctgaagag cttggcggcg aatgggctga ccgcttcctc gtgctttacg
1081 gtatcgccgc tcccgattcg cagcgcatcg ccttctatcg ccttcttgac gagttcttct
1141 gagcgggact ctggggttcg aaatgaccga ccaagcgacg cccaacctgc catcacgaga
1201 tttcgattcc accgccgcct tctatgaaag gttgggcttc ggaatcgttt tccgggacgc
1261 cggctggatg atcctccagc gcggggatct catgctggag ttcttcgccc accccaactt
1321 gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa
1381 agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca
1441 tgtctgtata ccgtcgacct ctagctagag cttggcgtaa tcatggtcat agctgtttcc
1501 tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg
1561 taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc
1621 cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg
1681 gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc
1741 ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac
1801 agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa
1861 ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca
1921 caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc
1981 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata
2041 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta
2101 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca
2161 gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga
2221 cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg
2281 tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg
2341 tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg
2401 caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag
2461 aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa
2521 cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat
2581 ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc
2641 tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc
2701 atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc
2761 tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc
2821 aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc
2881 catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt
2941 gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc
3001 ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa
3061 aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt
3121 atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg
3181 cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc
3241 gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa
3301 agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt
3361 gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt
3421 caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag
3481 ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta
3541 tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat
3601 aggggttccg cgcacatttc cccgaaaagt gccacctgac gtcgacggat cgggagatct
3661 cccgatcccc tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag
3721 tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt gcgcgagcaa aatttaagct
3781 acaacaaggc aaggcttgac cgacaattgc atgaagaatc tgcttagggt taggcgtttt
3841 gcgctgcttc gcgatgtacg ggccagatat acgcgttgac attgattatt gactagttat
3901 taatagtaat caattacggg gtcattagtt catagcccat atatggagtt ccgcgttaca
3961 taacttacgg taaatggccc gcctggctga ccgcccaacg acccccgccc attgacgtca
4021 ataatgacgt atgttcccat agtaacgcca atagggactt tccattgacg tcaatgggtg
4081 gagtatttac ggtaaactgc ccacttggca gtacatcaag tgtatcatat gccaagtacg
4141 ccccctattg acgtcaatga cggtaaatgg cccgcctggc attatgccca gtacatgacc
4201 ttatgggact ttcctacttg gcagtacatc tacgtattag tcatcgctat taccatggtg
4261 atgcggtttt ggcagtacat caatgggcgt ggatagcggt ttgactcacg gggatttcca
4321 agtctccacc ccattgacgt caatgggagt ttgttttggc accaaaatca acgggacttt
4381 ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg gcggtaggcg tgtacggtgg
4441 gaggtctata taagcagagc tctctggcta actagagaac ccactgctta ctggcttatc
4501 gaaattaata cgactcacta tagggagacc caagcttggt accgagctcg gatccactag
4561 taacggccgc cagtgtgctg gaattcggct tatacgcgga tccaccatgc ccctcaacgt
4621 tagcttcacc aacaggaact atgacctcga ctacgactcg gtgcagccgt atttctactg
4681 cgacgaggag gagaacttct accagcagca gcagcagagc gagctgcagc ccccggcgcc
4741 cagcgaggat atctggaaga aattcgagct gctgcccacc ccgcccctgt cccctagccg
4801 ccgctccggg ctctgctcgc cctcctacgt tgcggtcaca cccttctccc ttcggggaga
4861 caacgacggc ggtggcggga gcttctccac ggccgaccag ctggagatgg tgaccgagct
4921 gctgggagga gacatggtga accagagttt catctgcgac ccggacgacg agaccttcat
4981 caaaaacatc atcatccagg actgtatgtg gagcggcttc tcggccgccg ccaagctcgt
5041 ctcagagaag ctggcctcct accaggctgc gcgcaaagac agcggcagcc cgaaccccgc
5101 ccgcggccac agcgtctgct ccacctccag cttgtacctg caggatctga gcgccgccgc
5161 ctcagagtgc atcgacccct cggtggtctt cccctaccct ctcaacgaca gcagctcgcc
5221 caagtcctgc gcctcgcaag actccagcgc cttctctccg tcctcggatt ctctgctctc
5281 ctcgacggag tcctccccgc agggcagccc cgagcccctg gtgctccatg aggagacacc
5341 gcccaccacc agcagcgact ctgaggagga acaagaagat gaggaagaaa tcgatgttgt
5401 ttctgtggaa aagaggcagg ctcctggcaa aaggtcagag tctggatcac cttctgctgg
5461 aggccacagc aaacctcctc acagcccact ggtcctcaag aggtgccacg tctccacaca
5521 tcagcacaac tacgcagcgc ctccctccac tcggaaggac tatcctgctg ccaagagggt
5581 caagttggac agtgtcagag tcctgagaca gatcagcaac aaccgaaaat gcaccagccc
5641 caggtcctcg gacaccgagg agaatgtcaa gaggcgaaca cacaacgtct tggagcgcca
5701 gaggaggaac gagctaaaac ggagcttttt tgccctgcgt gaccagatcc cggagttgga
5761 aaacaatgaa aaggccccca aggtagttat ccttaaaaaa gccacagcat acatcctgtc
5821 cgtccaagca gaggagcaaa agctcatttc tgaagaggac ttgttgcgga aacgacgaga
5881 acagttgaaa cacaaacttg aacagctacg gaactcttgt gcgtaaccta ggatacgcaa
5941 gccgaattct gcagatatcc atcacactgg cggccgctcg agcatgcatc tagagggccc
6001 tattctatag tgtcacctaa atgctagagc tcgctgatca gcctcgactg tgccttctag
6061 ttgccagcca tctgttgttt gcccctcccc cgtgccttcc ttgaccctgg aaggtgccac
6121 tcccactgtc ctttcctaat aaaatgagga aattgcatcg cattgtctga gtaggtgtca
6181 ttctattctg gggggtgggg tggggcagga cagcaagggg gaggattggg aagacaatag
6241 caggcatgct ggggatgcgg tgggctctat ggcttctgag gcggaaagaa ccagctgggg
6301 ctctaggggg tatccccacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt
6361 tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt tcgctttctt
6421 cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggctccc
6481 tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg attagggtga
6541 tggttcacgt agtgggccat cgccctgata gacggttttt cgccctttga cgttggagtc
6601 cacgttcttt aatagtggac tcttgttcca aactggaaca acactcaacc ctatctcggt
6661 ctattctttt gatttataag ggattttgcc gatttcggcc tattggttaa aaaatgagct
6721 gatttaacaa aaatttaacg cgaattaatt ctgtggaatg tgtgtcagtt agggtgtgga
6781 aagtccccag gctccccagc aggcagaa
//
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