pCMV-3Tag-3a

LOCUS       pCMV-3Tag-3A                4214 bp ds-DNA     circular SYN 28-NOV-2013
DEFINITION  Mammalian expression vector for tagging proteins with a C-terminal 
            3xFLAG epitope. For other reading frames, use pCMV-3Tag-3B or 
            pCMV-3Tag-3C.
ACCESSION   .
VERSION     .
KEYWORDS    pCMV-3Tag-3A
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4214)
  AUTHORS   Agilent Technologies
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, February 4, 2020 from SnapGene Viewer 3.1.4
            http://www.snapgene.com
COMMENT     The cloned gene must include a Kozak sequence and start codon.
FEATURES             Location/Qualifiers
     source          1..4214
                     /organism="synthetic DNA construct"
                     /lab_host="Mammalian Cells"
                     /mol_type="other DNA"
     enhancer        66..370
                     /note="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        371..574
                     /note="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     promoter        620..638
                     /note="T3 promoter"
                     /note="promoter for bacteriophage T3 RNA polymerase"
     misc_feature    651..743
                     /note="MCS"
                     /note="multiple cloning site"
     CDS             744..815
                     /codon_start=1
                     /product="three tandem FLAG epitope tags"
                     /note="3xFLAG"
                     /translation="DYKDDDDKDYKDDDDKDYKDDDDK"
     misc_feature    831..841
                     /note="stop codons"
                     /note="stop codons in all three reading frames"
     promoter        complement(869..887)
                     /note="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     polyA_signal    922..1129
                     /note="bGH poly(A) signal"
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      complement(1136..1591)
                     /direction=LEFT
                     /note="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        1618..1720
                     /gene="bla"
                     /note="AmpR promoter"
     promoter        1724..2081
                     /note="SV40 promoter"
                     /note="SV40 enhancer and early promoter"
     CDS             2116..2910
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /note="NeoR/KanR"
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin)"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     misc_feature    3142..3189
                     /note="HSV TK poly(A) signal"
                     /note="herpesvirus thymidine kinase polyadenylation signal"
     rep_origin      3518..4106
                     /direction=RIGHT
                     /note="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 atgcattagt tattaatagt aatcaattac ggggtcatta gttcatagcc catatatgga
       61 gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca acgacccccg
      121 cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga ctttccattg
      181 acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc aagtgtatca
      241 tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc
      301 ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat tagtcatcgc
      361 tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc
      421 acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa
      481 tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag
      541 gcgtgtacgg tgggaggtct atataagcag agctggttta gtgaaccgtc agatccgcta
      601 gcgattacgc caagctcgaa attaaccctc actaaaggga acaaaagctg gagctccacc
      661 gcggtggcgg ccgctctagc ccgggcggat cccccgggct gcaggaattc gatatcaagc
      721 ttatcgatac cgtcgacctc gaggattaca aggatgacga cgataaggac tataaggacg
      781 atgatgacaa ggactacaaa gatgatgacg ataaataggg cccggtacct taattaatta
      841 aggtaccagg taagtgtacc caattcgccc tatagtgagt cgtattacaa ttcactcgat
      901 cggctcgctg atcagcctcg actgtgcctt ctagttgcca gccatctgtt gtttgcccct
      961 cccccgtgcc ttccttgacc ctggaaggtg ccactcccac tgtcctttcc taataaaatg
     1021 aggaaattgc atcgcattgt ctgagtaggt gtcattctat tctggggggt ggggtggggc
     1081 aggacagcaa gggggaggat tgggaagaca atagcaggca tgctggggaa cgcgtaaatt
     1141 gtaagcgtta atattttgtt aaaattcgcg ttaaattttt gttaaatcag ctcatttttt
     1201 aaccaatagg ccgaaatcgg caaaatccct tataaatcaa aagaatagac cgagataggg
     1261 ttgagtgttg ttccagtttg gaacaagagt ccactattaa agaacgtgga ctccaacgtc
     1321 aaagggcgaa aaaccgtcta tcagggcgat ggcccactac gtgaaccatc accctaatca
     1381 agttttttgg ggtcgaggtg ccgtaaagca ctaaatcgga accctaaagg gagcccccga
     1441 tttagagctt gacggggaaa gccggcgaac gtggcgagaa aggaagggaa gaaagcgaaa
     1501 ggagcgggcg ctagggcgct ggcaagtgta gcggtcacgc tgcgcgtaac caccacaccc
     1561 gccgcgctta atgcgccgct acagggcgcg tcaggtggca cttttcgggg aaatgtgcgc
     1621 ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa
     1681 taaccctgat aaatgcttca ataatattga aaaaggaaga atcctgaggc ggaaagaacc
     1741 agctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca gcaggcagaa
     1801 gtatgcaaag catgcatctc aattagtcag caaccaggtg tggaaagtcc ccaggctccc
     1861 cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccata gtcccgcccc
     1921 taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct
     1981 gactaatttt ttttatttat gcagaggccg aggccgcctc ggcctctgag ctattccaga
     2041 agtagtgagg aggctttttt ggaggcctag gcttttgcaa agatcgatca agagacagga
     2101 tgaggatcgt ttcgcatgat tgaacaagat ggattgcacg caggttctcc ggccgcttgg
     2161 gtggagaggc tattcggcta tgactgggca caacagacaa tcggctgctc tgatgccgcc
     2221 gtgttccggc tgtcagcgca ggggcgcccg gttctttttg tcaagaccga cctgtccggt
     2281 gccctgaatg aactgcaaga cgaggcagcg cggctatcgt ggctggccac gacgggcgtt
     2341 ccttgcgcag ctgtgctcga cgttgtcact gaagcgggaa gggactggct gctattgggc
     2401 gaagtgccgg ggcaggatct cctgtcatct caccttgctc ctgccgagaa agtatccatc
     2461 atggctgatg caatgcggcg gctgcatacg cttgatccgg ctacctgccc attcgaccac
     2521 caagcgaaac atcgcatcga gcgagcacgt actcggatgg aagccggtct tgtcgatcag
     2581 gatgatctgg acgaagaaca tcaggggctc gcgccagccg aactgttcgc caggctcaag
     2641 gcgagcatgc ccgacggcga ggatctcgtc gtgacccatg gcgatgcctg cttgccgaat
     2701 atcatggtgg aaaatggccg cttttctgga ttcatcgact gtggccggct gggtgtggcg
     2761 gaccgctatc aggacatagc gttggctacc cgtgatattg ctgaagaact tggcggcgaa
     2821 tgggctgacc gcttcctcgt gctttacggt atcgccgctc ccgattcgca gcgcatcgcc
     2881 ttctatcgcc ttcttgacga gttcttctga gcgggactct ggggttcgaa atgaccgacc
     2941 aagcgacgcc caacctgcca tcacgagatt tcgattccac cgccgccttc tatgaaaggt
     3001 tgggcttcgg aatcgttttc cgggacgccg gctggatgat cctccagcgc ggggatctca
     3061 tgctggagtt cttcgcccac cctaggggga ggctaactga aacacggaag gagacaatac
     3121 cggaaggaac ccgcgctatg acggcaataa aaagacagaa taaaacgcac ggtgttgggt
     3181 cgtttgttca taaacgcggg gttcggtccc agggctggca ctctgtcgat accccaccga
     3241 gaccccattg gggccaatac gcccgcgttt cttccttttc cccaccccac cccccaagtt
     3301 cgggtgaagg cccagggctc gcagccaacg tcggggcggc aggccctgcc atagcctcag
     3361 gttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg
     3421 tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact
     3481 gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg
     3541 taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc
     3601 aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata
     3661 ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta
     3721 catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc
     3781 ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg
     3841 ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac
     3901 agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg
     3961 taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt
     4021 atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct
     4081 cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg
     4141 ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata
     4201 accgtattac cgcc
//