pAAV-CaMKIIa-EGFP

价格:1100元

联系方式:I47-825O-882O

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载体基本信息

出品公司: Addgene
载体名称: pAAV-CaMKIIa-EGFP
质粒类型: 腺病毒载体;荧光报告载体
高拷贝/低拷贝: 低拷贝
克隆方法: 限制性内切酶,多克隆位点
启动子: CaMKIIa
载体大小: 6092 bp
5' 测序引物及序列: ATGCTGACGAAGGCTCGCGA
3' 测序引物及序列: GCATTAAAGCAGCGTATCCACATAGC
载体标签: --
载体抗性: 氨苄青霉素
筛选标记: EGFP
克隆菌株: Stbl3 或 NEB Stble
宿主细胞(系): 神经细胞
备注: --
产品目录号: # 50469
稳定性: 稳表达
组成型/诱导型: 组成型
病毒/非病毒: 腺病毒

载体质粒图谱和多克隆位点信息

pAAV-CaMKIIa-EGFP载体图谱



载体简介

载体序列

LOCUS       pAAV-CaMKIIa-EGFP	6092 bp 	DNA	SYN
DEFINITION  pAAV-CaMKIIa-EGFP
COMMENT     This file is created by Vector NTI
            http://www.biofeng.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTAUTHORNAME|biofeng.com|
COMMENT     VNTNAME|pAAV-CaMKIIa-EGFP|     
  ORGANISM  other sequences; artificial sequences; vectors.
FEATURES             Location/Qualifiers
     source          1..6092
                     /organism="pAAV-CaMKIIa-EGFP"
                     /mol_type="other DNA"
     CDS             1475..2194
                     /label="ORF frame 2"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLT
                     LKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK
                     DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKN
                     GIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDH
                     MVLLEFVTAAGITLGMDELYK*"
     gene            1478..2191
                     /label="EGFP"
                     /gene="EGFP"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLT
                     LKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK
                     DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKN
                     GIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDH
                     MVLLEFVTAAGITLGMDELYK*"
     misc_feature    complement(1520..1541)
                     /label="EGFP_N_primer"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLT
                     LKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK
                     DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKN
                     GIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDH
                     MVLLEFVTAAGITLGMDELYK*"
     misc_feature    1658..1687
                     /label="Y66 (EGFP)"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLT
                     LKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK
                     DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKN
                     GIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDH
                     MVLLEFVTAAGITLGMDELYK*"
     misc_feature    2128..2149
                     /label="EGFP_C_primer"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLT
                     LKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK
                     DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKN
                     GIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDH
                     MVLLEFVTAAGITLGMDELYK*"
     misc_feature    2221..2808
                     /label="WPRE"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLT
                     LKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK
                     DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKN
                     GIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDH
                     MVLLEFVTAAGITLGMDELYK*"
     CDS             2309..2920
                     /label="ORF frame 2"
                     /translation="MPLYHAIASRMAFIFSSLYKSWLLSLYEELWPVVRQRGVVCTVF
                     ADATPTGWGIATTCQLLSGTFAFPLPIATAELIAACLARCWTGARLLGTDNSVVLSGK
                     SSSFPWLLACVATWILRGTSFCYVPSALNPADLPSRGLLPALRPLPRLRLRPQTSRIS
                     LWAASPHRYRALLERSTGGIPVTPPQCLSWPWKLPLQCPPALS*"
     CDS             2322..2855
                     /label="ORF frame 3"
                     /translation="MLLLPVWLSFSPPCINPGCCLFMRSCGPLSGNVAWCALCLLTQP
                     PLVGALPPPVSSFPGLSLSPSLLPRRNSSPPALPAAGQGLGCWALTIPWCCRGNHRPF
                     LGCSPVLPPGFCAGRPSATSLRPSIQRTFLPAACCRLCGLFRVFAFALRRVGSPFGPP
                     PRIDTERCSRDLRVASL*"
     terminator      2837..3315
                     /label="hGH_PA_terminator"
                     /translation="MLLLPVWLSFSPPCINPGCCLFMRSCGPLSGNVAWCALCLLTQP
                     PLVGALPPPVSSFPGLSLSPSLLPRRNSSPPALPAAGQGLGCWALTIPWCCRGNHRPF
                     LGCSPVLPPGFCAGRPSATSLRPSIQRTFLPAACCRLCGLFRVFAFALRRVGSPFGPP
                     PRIDTERCSRDLRVASL*"
     rep_origin      3587..3893
                     /label="f1_origin"
                     /translation="MLLLPVWLSFSPPCINPGCCLFMRSCGPLSGNVAWCALCLLTQP
                     PLVGALPPPVSSFPGLSLSPSLLPRRNSSPPALPAAGQGLGCWALTIPWCCRGNHRPF
                     LGCSPVLPPGFCAGRPSATSLRPSIQRTFLPAACCRLCGLFRVFAFALRRVGSPFGPP
                     PRIDTERCSRDLRVASL*"
     misc_feature    4161..4183
                     /label="pGEX_3_primer"
                     /translation="MLLLPVWLSFSPPCINPGCCLFMRSCGPLSGNVAWCALCLLTQP
                     PLVGALPPPVSSFPGLSLSPSLLPRRNSSPPALPAAGQGLGCWALTIPWCCRGNHRPF
                     LGCSPVLPPGFCAGRPSATSLRPSIQRTFLPAACCRLCGLFRVFAFALRRVGSPFGPP
                     PRIDTERCSRDLRVASL*"
     promoter        4342..4370
                     /label="AmpR_promoter"
                     /translation="MLLLPVWLSFSPPCINPGCCLFMRSCGPLSGNVAWCALCLLTQP
                     PLVGALPPPVSSFPGLSLSPSLLPRRNSSPPALPAAGQGLGCWALTIPWCCRGNHRPF
                     LGCSPVLPPGFCAGRPSATSLRPSIQRTFLPAACCRLCGLFRVFAFALRRVGSPFGPP
                     PRIDTERCSRDLRVASL*"
     gene            4412..5272
                     /label="Ampicillin"
                     /gene="Ampicillin"
                     /translation="MLLLPVWLSFSPPCINPGCCLFMRSCGPLSGNVAWCALCLLTQP
                     PLVGALPPPVSSFPGLSLSPSLLPRRNSSPPALPAAGQGLGCWALTIPWCCRGNHRPF
                     LGCSPVLPPGFCAGRPSATSLRPSIQRTFLPAACCRLCGLFRVFAFALRRVGSPFGPP
                     PRIDTERCSRDLRVASL*"
     CDS             4412..5272
                     /label="ORF frame 2"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
     rep_origin      5427..6046
                     /label="pBR322_origin"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
ORIGIN
    1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc
   61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca
  121 actccatcac taggggttcc tgcggccgca cgcgtttaac attatggcct taggtcactt
  181 catctccatg gggttcttct tctgattttc tagaaaatga gatgggggtg cagagagctt
  241 cctcagtgac ctgcccaggg tcacatcaga aatgtcagag ctagaacttg aactcagatt
  301 actaatctta aattccatgc cttgggggca tgcaagtacg atatacagaa ggagtgaact
  361 cattagggca gatgaccaat gagtttagga aagaagagtc cagggcaggg tacatctaca
  421 ccacccgccc agccctgggt gagtccagcc acgttcacct cattatagtt gcctctctcc
  481 agtcctacct tgacgggaag cacaagcaga aactgggaca ggagccccag gagaccaaat
  541 cttcatggtc cctctgggag gatgggtggg gagagctgtg gcagaggcct caggaggggc
  601 cctgctgctc agtggtgaca gataggggtg agaaagcaga cagagtcatt ccgtcagcat
  661 tctgggtctg tttggtactt cttctcacgc taaggtggcg gtgtgatatg cacaatggct
  721 aaaaagcagg gagagctgga aagaaacaag gacagagaca gaggccaagt caaccagacc
  781 aattcccaga ggaagcaaag aaaccattac agagactaca agggggaagg gaaggagaga
  841 tgaattagct tcccctgtaa accttagaac ccagctgttg ccagggcaac ggggcaatac
  901 ctgtctcttc agaggagatg aagttgccag ggtaactaca tcctgtcttt ctcaaggacc
  961 atcccagaat gtggcaccca ctagccgtta ccatagcaac tgcctctttg ccccacttaa
 1021 tcccatcccg tctgttaaaa gggccctata gttggaggtg ggggaggtag gaagagcgat
 1081 gatcacttgt ggactaagtt tgttcgcatc cccttctcca accccctcag tacatcaccc
 1141 tgggggaaca gggtccactt gctcctgggc ccacacagtc ctgcagtatt gtgtatataa
 1201 ggccagggca aagaggagca ggttttaaag tgaaaggcag gcaggtgttg gggaggcagt
 1261 taccggggca acgggaacag ggcgtttcgg aggtggttgc catggggacc tggatgctga
 1321 cgaaggctcg cgaggctgtg agcagccaca gtgccctgct cagaagcccc aagctcgtca
 1381 gtcaagccgg ttctccgttt gcactcagga gcacgggcag gcgagtggcc cctagttctg
 1441 ggggcagctc tagagcggta ccggatccgc caccatggtg agcaagggcg aggagctgtt
 1501 caccggggtg gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc acaagttcag
 1561 cgtgtccggc gagggcgagg gcgatgccac ctacggcaag ctgaccctga agttcatctg
 1621 caccaccggc aagctgcccg tgccctggcc caccctcgtg accaccctga cctacggcgt
 1681 gcagtgcttc agccgctacc ccgaccacat gaagcagcac gacttcttca agtccgccat
 1741 gcccgaaggc tacgtccagg agcgcaccat cttcttcaag gacgacggca actacaagac
 1801 ccgcgccgag gtgaagttcg agggcgacac cctggtgaac cgcatcgagc tgaagggcat
 1861 cgacttcaag gaggacggca acatcctggg gcacaagctg gagtacaact acaacagcca
 1921 caacgtctat atcatggccg acaagcagaa gaacggcatc aaggtgaact tcaagatccg
 1981 ccacaacatc gaggacggca gcgtgcagct cgccgaccac taccagcaga acacccccat
 2041 cggcgacggc cccgtgctgc tgcccgacaa ccactacctg agcacccagt ccgccctgag
 2101 caaagacccc aacgagaagc gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg
 2161 gatcactctc ggcatggacg agctgtacaa gtaggaattc gatatcaagc ttatcgataa
 2221 tcaacctctg gattacaaaa tttgtgaaag attgactggt attcttaact atgttgctcc
 2281 ttttacgcta tgtggatacg ctgctttaat gcctttgtat catgctattg cttcccgtat
 2341 ggctttcatt ttctcctcct tgtataaatc ctggttgctg tctctttatg aggagttgtg
 2401 gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg
 2461 ttggggcatt gccaccacct gtcagctcct ttccgggact ttcgctttcc ccctccctat
 2521 tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt
 2581 gggcactgac aattccgtgg tgttgtcggg gaaatcatcg tcctttcctt ggctgctcgc
 2641 ctgtgttgcc acctggattc tgcgcgggac gtccttctgc tacgtccctt cggccctcaa
 2701 tccagcggac cttccttccc gcggcctgct gccggctctg cggcctcttc cgcgtcttcg
 2761 ccttcgccct cagacgagtc ggatctccct ttgggccgcc tccccgcatc gataccgagc
 2821 gctgctcgag agatctacgg gtggcatccc tgtgacccct ccccagtgcc tctcctggcc
 2881 ctggaagttg ccactccagt gcccaccagc cttgtcctaa taaaattaag ttgcatcatt
 2941 ttgtctgact aggtgtcctt ctataatatt atggggtgga ggggggtggt atggagcaag
 3001 gggcaagttg ggaagacaac ctgtagggcc tgcggggtct attgggaacc aagctggagt
 3061 gcagtggcac aatcttggct cactgcaatc tccgcctcct gggttcaagc gattctcctg
 3121 cctcagcctc ccgagttgtt gggattccag gcatgcatga ccaggctcag ctaatttttg
 3181 tttttttggt agagacgggg tttcaccata ttggccaggc tggtctccaa ctcctaatct
 3241 caggtgatct acccaccttg gcctcccaaa ttgctgggat tacaggcgtg aaccactgct
 3301 cccttccctg tccttctgat tttgtaggta accacgtgcg gaccgagcgg ccgcaggaac
 3361 ccctagtgat ggagttggcc actccctctc tgcgcgctcg ctcgctcact gaggccgggc
 3421 gaccaaaggt cgcccgacgc ccgggctttg cccgggcggc ctcagtgagc gagcgagcgc
 3481 gcagctgcct gcaggggcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt
 3541 cacaccgcat acgtcaaagc aaccatagta cgcgccctgt agcggcgcat taagcgcggc
 3601 gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc
 3661 tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa
 3721 tcgggggctc cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact
 3781 tgatttgggt gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt
 3841 gacgttggag tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa
 3901 ccctatctcg ggctattctt ttgatttata agggattttg ccgatttcgg cctattggtt
 3961 aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat taacgtttac
 4021 aattttatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagccccg
 4081 acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta
 4141 cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc
 4201 gaaacgcgcg agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat
 4261 aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat
 4321 ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata
 4381 aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct
 4441 tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa
 4501 agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa
 4561 cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt
 4621 taaagttctg ctatgtggcg cggtattatc ccgtattgac gccgggcaag agcaactcgg
 4681 tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca
 4741 tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa
 4801 cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt
 4861 gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc
 4921 cataccaaac gacgagcgtg acaccacgat gcctgtagca atggcaacaa cgttgcgcaa
 4981 actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga
 5041 ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc
 5101 tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga
 5161 tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga
 5221 acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga
 5281 ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat
 5341 ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt
 5401 ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct
 5461 gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc
 5521 ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc
 5581 aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc
 5641 gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc
 5701 gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg
 5761 aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata
 5821 cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta
 5881 tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc
 5941 ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg
 6001 atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt
 6061 cctggccttt tgctggcctt ttgctcacat gt
//

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