| 质粒名称: | pX601-mCherry |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 84039 |
| 存储实验室: | Yuet Wai Kan |
|
载体骨架基本信息 |
|
| 载体名称: | pX601 |
| 载体出品公司: | Feng Zhang's laboratory |
| 载体抗性: | 氨苄青霉素 |
| 空载体大小: | 7447 bp |
| 完整质粒大小: | 8122 bp |
| 载体修饰: | 在SaCas9下游插入樱桃 |
| 载体类型: | 哺乳细胞表达 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | mCherry |
| 启动子 | T2A |
| 标签/融合蛋白: | mCherry（插入件上的C端子） |
| 克隆方法: | 限制性内切酶 |
| 5' 克隆位点: | HindIII(未破坏) |
| 3' 克隆位点: | EcoR I (未破坏) |
| 5' 测序引物 | Cas9-HidIII-S: gccccgtcgtgaagagaagcttcatcc |
| 3' 测序引物 | mCherry –EcoR1-AS:cgtagaattcttacttgtacagctcgtccatgccgccggt |
|
文献引用方法 |
|
| 材料和方法部分: | pX601-mCherry was a gift from Yuet Wai Kan (Addgene plasmid # 84039 ; http://n2t.net/addgene:84039 ; RRID:Addgene_84039) |
| 参考文献部分: | Genome editing using CRISPR-Cas9 to create the HPFH genotype in HSPCs: An approach for treating sickle cell disease and beta-thalassemia. Ye L, Wang J, Tan Y, Beyer AI, Xie F, Muench MO, Kan YW. Proc Natl Acad Sci U S A. 2016 Sep 20;113(38):10661-5. doi: 10.1073/pnas.1612075113. Epub 2016 Sep 6. 10.1073/pnas.1612075113 PubMed 27601644 |
买家导航
pX601-mCherry载体 pX601mCherry质粒基本信息
pX601-mCherry质粒图谱 pX601mCherry载体图谱和pX601-mCherry载体序列 pX601mCherry质粒序列多克隆位点信息
pX601-mCherry质粒 pX601mCherry载体简介
pX601-mCherry质粒序列 pX601mCherry载体序列
LOCUS pX601-mCherry 8122 bp ds-DNA circular SYN 06-AUG-2018
DEFINITION Staphylococcus aureus (SaCas9) conjugated with mCherry.
ACCESSION .
VERSION .
KEYWORDS pX601-mCherry
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 8122)
AUTHORS Ye L, Wang J, Tan Y, Beyer AI, Xie F, Muench MO, Kan YW
TITLE Genome editing using CRISPR-Cas9 to create the HPFH genotype in
HSPCs: An approach for treating sickle cell disease and
beta-thalassemia.
JOURNAL Proc Natl Acad Sci U S A. 2016 Sep 20;113(38):10661-5. doi:
10.1073/pnas.1612075113. Epub 2016 Sep 6.
PUBMED 27601644
REFERENCE 2 (bases 1 to 8122)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Aug 6, 2018 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..8122
/organism="synthetic DNA construct"
/mol_type="other DNA"
repeat_region 1..130
/label=AAV2 ITR (alternate)
/note="Functional equivalent of wild-type AAV2 ITR"
enhancer 154..533
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 534..737
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind 688..708
/label=CMV-F
/note="Human CMV immediate early promoter, forward primer"
regulatory 756..765
/regulatory_class="other"
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
CDS 768..788
/codon_start=1
/product="nuclear localization signal of SV40 (simian virus
40) large T antigen"
/label=SV40 NLS
/translation="PKKKRKV"
CDS 813..3968
/codon_start=1
/product="Cas9 endonuclease from the Staphylococcus aureus
Type II CRISPR/Cas system"
/label=SaCas9
/note="generates RNA-guided double strand breaks in DNA"
/translation="KRNYILGLDIGITSVGYGIIDYETRDVIDAGVRLFKEANVENNEG
RRSKRGARRLKRRRRHRIQRVKKLLFDYNLLTDHSELSGINPYEARVKGLSQKLSEEEF
SAALLHLAKRRGVHNVNEVEEDTGNELSTKEQISRNSKALEEKYVAELQLERLKKDGEV
RGSINRFKTSDYVKEAKQLLKVQKAYHQLDQSFIDTYIDLLETRRTYYEGPGEGSPFGW
KDIKEWYEMLMGHCTYFPEELRSVKYAYNADLYNALNDLNNLVITRDENEKLEYYEKFQ
IIENVFKQKKKPTLKQIAKEILVNEEDIKGYRVTSTGKPEFTNLKVYHDIKDITARKEI
IENAELLDQIAKILTIYQSSEDIQEELTNLNSELTQEEIEQISNLKGYTGTHNLSLKAI
NLILDELWHTNDNQIAIFNRLKLVPKKVDLSQQKEIPTTLVDDFILSPVVKRSFIQSIK
VINAIIKKYGLPNDIIIELAREKNSKDAQKMINEMQKRNRQTNERIEEIIRTTGKENAK
YLIEKIKLHDMQEGKCLYSLEAIPLEDLLNNPFNYEVDHIIPRSVSFDNSFNNKVLVKQ
EENSKKGNRTPFQYLSSSDSKISYETFKKHILNLAKGKGRISKTKKEYLLEERDINRFS
VQKDFINRNLVDTRYATRGLMNLLRSYFRVNNLDVKVKSINGGFTSFLRRKWKFKKERN
KGYKHHAEDALIIANADFIFKEWKKLDKAKKVMENQMFEEKQAESMPEIETEQEYKEIF
ITPHQIKHIKDFKDYKYSHRVDKKPNRELINDTLYSTRKDDKGNTLIVNNLNGLYDKDN
DKLKKLINKSPEKLLMYHHDPQTYQKLKLIMEQYGDEKNPLYKYYEETGNYLTKYSKKD
NGPVIKKIKYYGNKLNAHLDITDDYPNSRNKVVKLSLKPYRFDVYLDNGVYKFVTVKNL
DVIKKENYYEVNSKCYEEAKKLKKISNQAEFIASFYNNDLIKINGELYRVIGVNNDLLN
RIEVNMIDITYREYLENMNDKRPPRIIKTIASKTQSIKKYSTDILGNLYEVKSKKHPQI
IKKG"
CDS 3969..4016
/codon_start=1
/product="bipartite nuclear localization signal from
nucleoplasmin"
/label=nucleoplasmin NLS
/translation="KRPAATKKAGQAKKKK"
CDS 4017..4070
/codon_start=1
/product="2A peptide from Thosea asigna virus capsid
protein"
/label=T2A
/note="Eukaryotic ribosomes fail to insert a peptide bond
between the Gly and Pro residues, yielding separate
polypeptides."
/translation="EGRGSLLTCGDVEENPGP"
CDS 4071..4781
/codon_start=1
/product="monomeric derivative of DsRed fluorescent protein
(Shaner et al., 2004)"
/label=mCherry
/note="mammalian codon-optimized"
/translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
EGRHSTGGMDELYK"
primer_bind complement(4216..4234)
/label=mCherry-R
/note="mCherry, reverse primer"
primer_bind 4484..4503
/label=mCherry-F
/note="mCherry, forward primer"
primer_bind complement(4806..4823)
/label=BGH-rev
/note="Bovine growth hormone terminator, reverse primer.
Also called BGH reverse"
polyA_signal 4812..5019
/label=bGH poly(A) signal
/note="bovine growth hormone polyadenylation signal"
promoter 5027..5267
/label=U6 promoter
/note="RNA polymerase III promoter for human U6 snRNA"
primer_bind 5027..5047
/label=hU6-F
/note="Human U6 promoter, forward primer"
primer_bind 5198..5217
/label=LKO.1 5'
/note="Human U6 promoter, forward primer"
misc_RNA 5296..5371
/label=Sa gRNA scaffold
/note="guide RNA scaffold for the Staphylococcus aureus
CRISPR/Cas9 system"
repeat_region 5385..5525
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
repeat_region 5385..5514
/label=AAV2 ITR
rep_origin 5600..6055
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(5687..5706)
/label=F1ori-R
/note="F1 origin, reverse primer"
primer_bind 5897..5918
/label=F1ori-F
/note="F1 origin, forward primer"
primer_bind complement(6072..6091)
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
primer_bind 6191..6213
/label=pGEX 3'
/note="pGEX vectors, reverse primer"
primer_bind complement(6251..6269)
/label=pBRforEco
/note="pBR322 vectors, upsteam of EcoRI site, forward
primer"
promoter 6337..6441
/gene="bla"
/label=AmpR promoter
CDS 6442..7302
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind complement(6660..6679)
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
rep_origin 7473..8061
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 7962..7981
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
ORIGIN
1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
121 aggggttcct gcggcctcta gactcgaggc gttgacattg attattgact agttattaat
181 agtaatcaat tacggggtca ttagttcata gcccatatat ggagttccgc gttacataac
241 ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa
301 tgacgtatgt tcccatagta acgccaatag ggactttcca ttgacgtcaa tgggtggagt
361 atttacggta aactgcccac ttggcagtac atcaagtgta tcatatgcca agtacgcccc
421 ctattgacgt caatgacggt aaatggcccg cctggcatta tgcccagtac atgaccttat
481 gggactttcc tacttggcag tacatctacg tattagtcat cgctattacc atggtgatgc
541 ggttttggca gtacatcaat gggcgtggat agcggtttga ctcacgggga tttccaagtc
601 tccaccccat tgacgtcaat gggagtttgt tttggcacca aaatcaacgg gactttccaa
661 aatgtcgtaa caactccgcc ccattgacgc aaatgggcgg taggcgtgta cggtgggagg
721 tctatataag cagagctctc tggctaacta ccggtgccac catggcccca aagaagaagc
781 ggaaggtcgg tatccacgga gtcccagcag ccaagcggaa ctacatcctg ggcctggaca
841 tcggcatcac cagcgtgggc tacggcatca tcgactacga gacacgggac gtgatcgatg
901 ccggcgtgcg gctgttcaaa gaggccaacg tggaaaacaa cgagggcagg cggagcaaga
961 gaggcgccag aaggctgaag cggcggaggc ggcatagaat ccagagagtg aagaagctgc
1021 tgttcgacta caacctgctg accgaccaca gcgagctgag cggcatcaac ccctacgagg
1081 ccagagtgaa gggcctgagc cagaagctga gcgaggaaga gttctctgcc gccctgctgc
1141 acctggccaa gagaagaggc gtgcacaacg tgaacgaggt ggaagaggac accggcaacg
1201 agctgtccac caaagagcag atcagccgga acagcaaggc cctggaagag aaatacgtgg
1261 ccgaactgca gctggaacgg ctgaagaaag acggcgaagt gcggggcagc atcaacagat
1321 tcaagaccag cgactacgtg aaagaagcca aacagctgct gaaggtgcag aaggcctacc
1381 accagctgga ccagagcttc atcgacacct acatcgacct gctggaaacc cggcggacct
1441 actatgaggg acctggcgag ggcagcccct tcggctggaa ggacatcaaa gaatggtacg
1501 agatgctgat gggccactgc acctacttcc ccgaggaact gcggagcgtg aagtacgcct
1561 acaacgccga cctgtacaac gccctgaacg acctgaacaa tctcgtgatc accagggacg
1621 agaacgagaa gctggaatat tacgagaagt tccagatcat cgagaacgtg ttcaagcaga
1681 agaagaagcc caccctgaag cagatcgcca aagaaatcct cgtgaacgaa gaggatatta
1741 agggctacag agtgaccagc accggcaagc ccgagttcac caacctgaag gtgtaccacg
1801 acatcaagga cattaccgcc cggaaagaga ttattgagaa cgccgagctg ctggatcaga
1861 ttgccaagat cctgaccatc taccagagca gcgaggacat ccaggaagaa ctgaccaatc
1921 tgaactccga gctgacccag gaagagatcg agcagatctc taatctgaag ggctataccg
1981 gcacccacaa cctgagcctg aaggccatca acctgatcct ggacgagctg tggcacacca
2041 acgacaacca gatcgctatc ttcaaccggc tgaagctggt gcccaagaag gtggacctgt
2101 cccagcagaa agagatcccc accaccctgg tggacgactt catcctgagc cccgtcgtga
2161 agagaagctt catccagagc atcaaagtga tcaacgccat catcaagaag tacggcctgc
2221 ccaacgacat cattatcgag ctggcccgcg agaagaactc caaggacgcc cagaaaatga
2281 tcaacgagat gcagaagcgg aaccggcaga ccaacgagcg gatcgaggaa atcatccgga
2341 ccaccggcaa agagaacgcc aagtacctga tcgagaagat caagctgcac gacatgcagg
2401 aaggcaagtg cctgtacagc ctggaagcca tccctctgga agatctgctg aacaacccct
2461 tcaactatga ggtggaccac atcatcccca gaagcgtgtc cttcgacaac agcttcaaca
2521 acaaggtgct cgtgaagcag gaagaaaaca gcaagaaggg caaccggacc ccattccagt
2581 acctgagcag cagcgacagc aagatcagct acgaaacctt caagaagcac atcctgaatc
2641 tggccaaggg caagggcaga atcagcaaga ccaagaaaga gtatctgctg gaagaacggg
2701 acatcaacag gttctccgtg cagaaagact tcatcaaccg gaacctggtg gataccagat
2761 acgccaccag aggcctgatg aacctgctgc ggagctactt cagagtgaac aacctggacg
2821 tgaaagtgaa gtccatcaat ggcggcttca ccagctttct gcggcggaag tggaagttta
2881 agaaagagcg gaacaagggg tacaagcacc acgccgagga cgccctgatc attgccaacg
2941 ccgatttcat cttcaaagag tggaagaaac tggacaaggc caaaaaagtg atggaaaacc
3001 agatgttcga ggaaaagcag gccgagagca tgcccgagat cgaaaccgag caggagtaca
3061 aagagatctt catcaccccc caccagatca agcacattaa ggacttcaag gactacaagt
3121 acagccaccg ggtggacaag aagcctaata gagagctgat taacgacacc ctgtactcca
3181 cccggaagga cgacaagggc aacaccctga tcgtgaacaa tctgaacggc ctgtacgaca
3241 aggacaatga caagctgaaa aagctgatca acaagagccc cgaaaagctg ctgatgtacc
3301 accacgaccc ccagacctac cagaaactga agctgattat ggaacagtac ggcgacgaga
3361 agaatcccct gtacaagtac tacgaggaaa ccgggaacta cctgaccaag tactccaaaa
3421 aggacaacgg ccccgtgatc aagaagatta agtattacgg caacaaactg aacgcccatc
3481 tggacatcac cgacgactac cccaacagca gaaacaaggt cgtgaagctg tccctgaagc
3541 cctacagatt cgacgtgtac ctggacaatg gcgtgtacaa gttcgtgacc gtgaagaatc
3601 tggatgtgat caaaaaagaa aactactacg aagtgaatag caagtgctat gaggaagcta
3661 agaagctgaa gaagatcagc aaccaggccg agtttatcgc ctccttctac aacaacgatc
3721 tgatcaagat caacggcgag ctgtatagag tgatcggcgt gaacaacgac ctgctgaacc
3781 ggatcgaagt gaacatgatc gacatcacct accgcgagta cctggaaaac atgaacgaca
3841 agaggccccc caggatcatt aagacaatcg cctccaagac ccagagcatt aagaagtaca
3901 gcacagacat tctgggcaac ctgtatgaag tgaaatctaa gaagcaccct cagatcatca
3961 aaaagggcaa aaggccggcg gccacgaaaa aggccggcca ggcaaaaaag aaaaaggagg
4021 gcagaggatc cctgctaaca tgtggtgacg tcgaggagaa tcctggccca atggtgagca
4081 agggcgagga ggataacatg gccatcatca aggagttcat gcgcttcaag gtgcacatgg
4141 agggctccgt gaacggccac gagttcgaga tcgagggcga gggcgagggc cgcccctacg
4201 agggcaccca gaccgccaag ctgaaggtga ccaagggtgg ccccctgccc ttcgcctggg
4261 acatcctgtc ccctcagttc atgtacggct ccaaggccta cgtgaagcac cccgccgaca
4321 tccccgacta cttgaagctg tccttccccg agggcttcaa gtgggagcgc gtgatgaact
4381 tcgaggacgg cggcgtggtg accgtgaccc aggactcctc cctgcaggac ggcgagttca
4441 tctacaaggt gaagctgcgc ggcaccaact tcccctccga cggccccgta atgcagaaga
4501 agaccatggg ctgggaggcc tcctccgagc ggatgtaccc cgaggacggc gccctgaagg
4561 gcgagatcaa gcagaggctg aagctgaagg acggcggcca ctacgacgct gaggtcaaga
4621 ccacctacaa ggccaagaag cccgtgcagc tgcccggcgc ctacaacgtc aacatcaagt
4681 tggacatcac ctcccacaac gaggactaca ccatcgtgga acagtacgaa cgcgccgagg
4741 gccgccactc caccggcggc atggacgagc tgtacaagta agaattccta gagctcgctg
4801 atcagcctcg actgtgcctt ctagttgcca gccatctgtt gtttgcccct cccccgtgcc
4861 ttccttgacc ctggaaggtg ccactcccac tgtcctttcc taataaaatg aggaaattgc
4921 atcgcattgt ctgagtaggt gtcattctat tctggggggt ggggtggggc aggacagcaa
4981 gggggaggat tgggaagaga atagcaggca tgctggggag gtacctgagg gcctatttcc
5041 catgattcct tcatatttgc atatacgata caaggctgtt agagagataa ttggaattaa
5101 tttgactgta aacacaaaga tattagtaca aaatacgtga cgtagaaagt aataatttct
5161 tgggtagttt gcagttttaa aattatgttt taaaatggac tatcatatgc ttaccgtaac
5221 ttgaaagtat ttcgatttct tggctttata tatcttgtgg aaaggacgaa acaccggaga
5281 ccacggcagg tctcagtttt agtactctgg aaacagaatc tactaaaaca aggcaaaatg
5341 ccgtgtttat ctcgtcaact tgttggcgag atttttgcgg ccgcaggaac ccctagtgat
5401 ggagttggcc actccctctc tgcgcgctcg ctcgctcact gaggccgggc gaccaaaggt
5461 cgcccgacgc ccgggctttg cccgggcggc ctcagtgagc gagcgagcgc gcagctgcct
5521 gcaggggcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat
5581 acgtcaaagc aaccatagta cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg
5641 gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc
5701 ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc
5761 cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgatttgggt
5821 gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag
5881 tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa ccctatctcg
5941 ggctattctt ttgatttata agggattttg ccgatttcgg cctattggtt aaaaaatgag
6001 ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat taacgtttac aattttatgg
6061 tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagccccg acacccgcca
6121 acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct
6181 gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg
6241 agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt
6301 tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt
6361 ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa
6421 taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt
6481 tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat
6541 gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag
6601 atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg
6661 ctatgtggcg cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata
6721 cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat
6781 ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc
6841 aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg
6901 ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac
6961 gacgagcgtg acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact
7021 ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa
7081 gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct
7141 ggagccggtg agcgtggaag ccgcggtatc attgcagcac tggggccaga tggtaagccc
7201 tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga
7261 cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac
7321 tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag
7381 atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg
7441 tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc
7501 tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag
7561 ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc
7621 cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac
7681 ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc
7741 gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt
7801 tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt
7861 gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc
7921 ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt
7981 tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca
8041 ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt
8101 tgctggcctt ttgctcacat gt
//
