pC0050-CMV-dPspCas13b-longlinker-ADAR2DD(wt)

LOCUS       pC0050-CMV-dPspCas13b-longlinker-ADAR2DD(wt)                9864 bp ds-DNA     circular SYN 06-AUG-2018
DEFINITION  dPspCas13b-ADAR2DD(wt) fusion that can be used to selectively edit 
            adenosine to inosine in RNA molecules when used in conjuction with a
            guide RNA. Longer linker than pC0039..
ACCESSION   .
VERSION     .
KEYWORDS    pC0050-CMV-dPspCas13b-longlinker-ADAR2DD(wt)
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9864)
  AUTHORS   Cox DBT, Gootenberg JS, Abudayyeh OO, Franklin B, Kellner MJ, Joung 
            J, Zhang F
  TITLE     RNA editing with CRISPR-Cas13.
  JOURNAL   Science. 2017 Oct 25. pii: eaaq0180. doi: 10.1126/science.aaq0180.
  PUBMED    29070703
REFERENCE   2  (bases 1 to 9864)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Aug 6, 2018 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..9864
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     rep_origin      96..231
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     158..177
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             312..1106
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(366..385)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     976..995
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    1280..1401
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1317..1336)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1371..1390
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(1450..1466)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1450..1466)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1463..1485)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1474..1490
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(1498..1528)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1543..1564
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1681..1698)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1852..2440)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1932..1951)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(2611..3471)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3234..3253
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3472..3576)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(3651..3670)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        3842..4221
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        4222..4425
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     4376..4396
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     4470..4489
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        4470..4488
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             7812..7838
                     /codon_start=1
                     /product="nuclear export signal from the HIV Rev protein 
                     (Fischer et al., 1995)"
                     /label=NES
                     /translation="LPPLERLTL"
     primer_bind     complement(9062..9079)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    9068..9292
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      9338..9766
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9425..9444)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9635..9656
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(9775..9795)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        9780..245
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
ORIGIN
        1 ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt
       61 agtcagcaac catagtcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt
      121 ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg
      181 cctctgcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc ctaggctttt
      241 gcaaaaagct cccgggagct tgtatatcca ttttcggatc tgatcaagag acaggatgag
      301 gatcgtttcg catgattgaa caagatggat tgcacgcagg ttctccggcc gcttgggtgg
      361 agaggctatt cggctatgac tgggcacaac agacaatcgg ctgctctgat gccgccgtgt
      421 tccggctgtc agcgcagggg cgcccggttc tttttgtcaa gaccgacctg tccggtgccc
      481 tgaatgaact gcaggacgag gcagcgcggc tatcgtggct ggccacgacg ggcgttcctt
      541 gcgcagctgt gctcgacgtt gtcactgaag cgggaaggga ctggctgcta ttgggcgaag
      601 tgccggggca ggatctcctg tcatctcacc ttgctcctgc cgagaaagta tccatcatgg
      661 ctgatgcaat gcggcggctg catacgcttg atccggctac ctgcccattc gaccaccaag
      721 cgaaacatcg catcgagcga gcacgtactc ggatggaagc cggtcttgtc gatcaggatg
      781 atctggacga agagcatcag gggctcgcgc cagccgaact gttcgccagg ctcaaggcgc
      841 gcatgcccga cggcgaggat ctcgtcgtga cccatggcga tgcctgcttg ccgaatatca
      901 tggtggaaaa tggccgcttt tctggattca tcgactgtgg ccggctgggt gtggcggacc
      961 gctatcagga catagcgttg gctacccgtg atattgctga agagcttggc ggcgaatggg
     1021 ctgaccgctt cctcgtgctt tacggtatcg ccgctcccga ttcgcagcgc atcgccttct
     1081 atcgccttct tgacgagttc ttctgagcgg gactctgggg ttcgaaatga ccgaccaagc
     1141 gacgcccaac ctgccatcac gagatttcga ttccaccgcc gccttctatg aaaggttggg
     1201 cttcggaatc gttttccggg acgccggctg gatgatcctc cagcgcgggg atctcatgct
     1261 ggagttcttc gcccacccca acttgtttat tgcagcttat aatggttaca aataaagcaa
     1321 tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc
     1381 caaactcatc aatgtatctt atcatgtctg tataccgtcg acctctagct agagcttggc
     1441 gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa
     1501 catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac
     1561 attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca
     1621 ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc
     1681 ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc
     1741 aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc
     1801 aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag
     1861 gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc
     1921 gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt
     1981 tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct
     2041 ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg
     2101 ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct
     2161 tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat
     2221 tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg
     2281 ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa
     2341 aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt
     2401 ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc
     2461 tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt
     2521 atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta
     2581 aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat
     2641 ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac
     2701 tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg
     2761 ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag
     2821 tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt
     2881 aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt
     2941 gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt
     3001 tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt
     3061 cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct
     3121 tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt
     3181 ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac
     3241 cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa
     3301 actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa
     3361 ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca
     3421 aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct
     3481 ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga
     3541 atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc
     3601 tgacgtcgac ggatcgggag atctcccgat cccctatggt gcactctcag tacaatctgc
     3661 tctgatgccg catagttaag ccagtatctg ctccctgctt gtgtgttgga ggtcgctgag
     3721 tagtgcgcga gcaaaattta agctacaaca aggcaaggct tgaccgacaa ttgcatgaag
     3781 aatctgctta gggttaggcg ttttgcgctg cttcgcgatg tacgggccag atatacgcgt
     3841 tgacattgat tattgactag ttattaatag taatcaatta cggggtcatt agttcatagc
     3901 ccatatatgg agttccgcgt tacataactt acggtaaatg gcccgcctgg ctgaccgccc
     3961 aacgaccccc gcccattgac gtcaataatg acgtatgttc ccatagtaac gccaataggg
     4021 actttccatt gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt ggcagtacat
     4081 caagtgtatc atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc
     4141 tggcattatg cccagtacat gaccttatgg gactttccta cttggcagta catctacgta
     4201 ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag
     4261 cggtttgact cacggggatt tccaagtctc caccccattg acgtcaatgg gagtttgttt
     4321 tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa
     4381 atgggcggta ggcgtgtacg gtgggaggtc tatataagca gagctctctg gctaactaga
     4441 gaacccactg cttactggct tatcgaaatt aatacgactc actataggga gacccaagct
     4501 ggctagcgtt taaacttaag cttgccacca tgaacatccc cgctctggtg gaaaaccaga
     4561 agaagtactt tggcacctac agcgtgatgg ccatgctgaa cgctcagacc gtgctggacc
     4621 acatccagaa ggtggccgat attgagggcg agcagaacga gaacaacgag aatctgtggt
     4681 ttcaccccgt gatgagccac ctgtacaacg ccaagaacgg ctacgacaag cagcccgaga
     4741 aaaccatgtt catcatcgag cggctgcaga gctacttccc attcctgaag atcatggccg
     4801 agaaccagag agagtacagc aacggcaagt acaagcagaa ccgcgtggaa gtgaacagca
     4861 acgacatctt cgaggtgctg aagcgcgcct tcggcgtgct gaagatgtac agggacctga
     4921 ccaacgcata caagacctac gaggaaaagc tgaacgacgg ctgcgagttc ctgaccagca
     4981 cagagcaacc tctgagcggc atgatcaaca actactacac agtggccctg cggaacatga
     5041 acgagagata cggctacaag acagaggacc tggccttcat ccaggacaag cggttcaagt
     5101 tcgtgaagga cgcctacggc aagaaaaagt cccaagtgaa taccggattc ttcctgagcc
     5161 tgcaggacta caacggcgac acacagaaga agctgcacct gagcggagtg ggaatcgccc
     5221 tgctgatctg cctgttcctg gacaagcagt acatcaacat ctttctgagc aggctgccca
     5281 tcttctccag ctacaatgcc cagagcgagg aacggcggat catcatcaga tccttcggca
     5341 tcaacagcat caagctgccc aaggaccgga tccacagcga gaagtccaac aagagcgtgg
     5401 ccatggatat gctcaacgaa gtgaagcggt gccccgacga gctgttcaca acactgtctg
     5461 ccgagaagca gtcccggttc agaatcatca gcgacgacca caatgaagtg ctgatgaagc
     5521 ggagcagcga cagattcgtg cctctgctgc tgcagtatat cgattacggc aagctgttcg
     5581 accacatcag gttccacgtg aacatgggca agctgagata cctgctgaag gccgacaaga
     5641 cctgcatcga cggccagacc agagtcagag tgatcgagca gcccctgaac ggcttcggca
     5701 gactggaaga ggccgagaca atgcggaagc aagagaacgg caccttcggc aacagcggca
     5761 tccggatcag agacttcgag aacatgaagc gggacgacgc caatcctgcc aactatccct
     5821 acatcgtgga cacctacaca cactacatcc tggaaaacaa caaggtcgag atgtttatca
     5881 acgacaaaga ggacagcgcc ccactgctgc ccgtgatcga ggatgataga tacgtggtca
     5941 agacaatccc cagctgccgg atgagcaccc tggaaattcc agccatggcc ttccacatgt
     6001 ttctgttcgg cagcaagaaa accgagaagc tgatcgtgga cgtgcacaac cggtacaaga
     6061 gactgttcca ggccatgcag aaagaagaag tgaccgccga gaatatcgcc agcttcggaa
     6121 tcgccgagag cgacctgcct cagaagatcc tggatctgat cagcggcaat gcccacggca
     6181 aggatgtgga cgccttcatc agactgaccg tggacgacat gctgaccgac accgagcgga
     6241 gaatcaagag attcaaggac gaccggaagt ccattcggag cgccgacaac aagatgggaa
     6301 agagaggctt caagcagatc tccacaggca agctggccga cttcctggcc aaggacatcg
     6361 tgctgtttca gcccagcgtg aacgatggcg agaacaagat caccggcctg aactaccgga
     6421 tcatgcagag cgccattgcc gtgtacgata gcggcgacga ttacgaggcc aagcagcagt
     6481 tcaagctgat gttcgagaag gcccggctga tcggcaaggg cacaacagag cctcatccat
     6541 ttctgtacaa ggtgttcgcc cgcagcatcc ccgccaatgc cgtcgagttc tacgagcgct
     6601 acctgatcga gcggaagttc tacctgaccg gcctgtccaa cgagatcaag aaaggcaaca
     6661 gagtggatgt gcccttcatc cggcgggacc agaacaagtg gaaaacaccc gccatgaaga
     6721 ccctgggcag aatctacagc gaggatctgc ccgtggaact gcccagacag atgttcgaca
     6781 atgagatcaa gtcccacctg aagtccctgc cacagatgga aggcatcgac ttcaacaatg
     6841 ccaacgtgac ctatctgatc gccgagtaca tgaagagagt gctggacgac gacttccaga
     6901 ccttctacca gtggaaccgc aactaccggt acatggacat gcttaagggc gagtacgaca
     6961 gaaagggctc cctgcagcac tgcttcacca gcgtggaaga gagagaaggc ctctggaaag
     7021 agcgggcctc cagaacagag cggtacagaa agcaggccag caacaagatc cgcagcaacc
     7081 ggcagatgag aaacgccagc agcgaagaga tcgagacaat cctggataag cggctgagca
     7141 acagccggaa cgagtaccag aaaagcgaga aagtgatccg gcgctacaga gtgcaggatg
     7201 ccctgctgtt tctgctggcc aaaaagaccc tgaccgaact ggccgatttc gacggcgaga
     7261 ggttcaaact gaaagaaatc atgcccgacg ccgagaaggg aatcctgagc gagatcatgc
     7321 ccatgagctt caccttcgag aaaggcggca agaagtacac catcaccagc gagggcatga
     7381 agctgaagaa ctacggcgac ttctttgtgc tggctagcga caagaggatc ggcaacctgc
     7441 tggaactcgt gggcagcgac atcgtgtcca aagaggatat catggaagag ttcaacaaat
     7501 acgaccagtg caggcccgag atcagctcca tcgtgttcaa cctggaaaag tgggccttcg
     7561 acacataccc cgagctgtct gccagagtgg accgggaaga gaaggtggac ttcaagagca
     7621 tcctgaaaat cctgctgaac aacaagaaca tcaacaaaga gcagagcgac atcctgcgga
     7681 agatccggaa cgccttcgat gcaaacaatt accccgacaa aggcgtggtg gaaatcaagg
     7741 ccctgcctga gatcgccatg agcatcaaga aggcctttgg ggagtacgcc atcatgaagg
     7801 gaagcctgca gctgcctcca cttgaaagac tgacactggg atccggagga ggtggaagcc
     7861 agctgcattt accgcaggtt ttagctgacg ctgtctcacg cctggtcctg ggtaagtttg
     7921 gtgacctgac cgacaacttc tcctcccctc acgctcgcag aaaagtgctg gctggagtcg
     7981 tcatgacaac aggcacagat gttaaagatg ccaaggtgat aagtgtttct acaggaacaa
     8041 aatgtattaa tggtgaatac atgagtgatc gtggccttgc attaaatgac tgccatgcag
     8101 aaataatatc tcggagatcc ttgctcagat ttctttatac acaacttgag ctttacttaa
     8161 ataacaaaga tgatcaaaaa agatccatct ttcagaaatc agagcgaggg gggtttaggc
     8221 tgaaggagaa tgtccagttt catctgtaca tcagcacctc tccctgtgga gatgccagaa
     8281 tcttctcacc acatgagcca atcctggaag aaccagcaga tagacaccca aatcgtaaag
     8341 caagaggaca gctacggacc aaaatagagt ctggtgaggg gacgattcca gtgcgctcca
     8401 atgcgagcat ccaaacgtgg gacggggtgc tgcaagggga gcggctgctc accatgtcct
     8461 gcagtgacaa gattgcacgc tggaacgtgg tgggcatcca gggatcactg ctcagcattt
     8521 tcgtggagcc catttacttc tcgagcatca tcctgggcag cctttaccac ggggaccacc
     8581 tttccagggc catgtaccag cggatctcca acatagagga cctgccacct ctctacaccc
     8641 tcaacaagcc tttgctcagt ggcatcagca atgcagaagc acggcagcca gggaaggccc
     8701 ccaacttcag tgtcaactgg acggtaggcg actccgctat tgaggtcatc aacgccacga
     8761 ctgggaagga tgagctgggc cgcgcgtccc gcctgtgtaa gcacgcgttg tactgtcgct
     8821 ggatgcgtgt gcacggcaag gttccctccc acttactacg ctccaagatt accaagccca
     8881 acgtgtacca tgagtccaag ctggcggcaa aggagtacca ggccgccaag gcgcgtctgt
     8941 tcacagcctt catcaaggcg gggctggggg cctgggtgga gaagcccacc gagcaggacc
     9001 agttctcact cacgtaagcg gccgctcgag tctagagggc ccgtttaaac ccgctgatca
     9061 gcctcgactg tgccttctag ttgccagcca tctgttgttt gcccctcccc cgtgccttcc
     9121 ttgaccctgg aaggtgccac tcccactgtc ctttcctaat aaaatgagga aattgcatcg
     9181 cattgtctga gtaggtgtca ttctattctg gggggtgggg tggggcagga cagcaagggg
     9241 gaggattggg aagacaatag caggcatgct ggggatgcgg tgggctctat ggcttctgag
     9301 gcggaaagaa ccagctgggg ctctaggggg tatccccacg cgccctgtag cggcgcatta
     9361 agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg
     9421 cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa
     9481 gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc
     9541 aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt
     9601 cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca
     9661 acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc gatttcggcc
     9721 tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattaatt ctgtggaatg
     9781 tgtgtcagtt agggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca
     9841 tgcatctcaa ttagtcagca acca
//