pJL-TRBO

LOCUS       pJL-TRBO               10608 bp ds-DNA     circular SYN 10-JAN-2018
DEFINITION  An improved agroinfection-compatible Tobacco mosaic virus 
            (TMV)-based transient expression vector that lacks the TMV CP gene 
            coding sequence..
ACCESSION   .
VERSION     .
KEYWORDS    pJL-TRBO
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10608)
  AUTHORS   Lindbo JA
  TITLE     TRBO: a high-efficiency tobacco mosaic virus RNA-based 
            overexpression vector.
  JOURNAL   Plant Physiol. 2007 Dec;145(4):1232-40. Epub 2007 Aug 24.
  PUBMED    17720752
COMMENT     This file is created by Vector NTI
            http://www.biofeng.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTAUTHORNAME|biofeng.com|
COMMENT     VNTNAME|pJL-TRBO|
FEATURES             Location/Qualifiers
     source          1..10608
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    12..67
                     /label=TMV Omega
                     /note="translational enhancer from the tobacco mosaic virus
                     5'-leader sequence (Gallie et al., 1988)"
     misc_feature    6374..6398
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             complement(6550..7698)
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MAQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     CDS             complement(7997..8791)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     rep_origin      9003..9534
                     /label=oriV
                     /note="incP origin of replication"
     rep_origin      9011..9625
                     /label=oriV
                     /note="origin of replication for the bacterial F plasmid"
     misc_feature    9715..9739
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     primer_bind     9819..9835
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     9820..9836
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 gtatttttac aacaattacc aacaacaaca aacaacagac aacattacaa ttactattta
       61 caattacaat ggcatacaca cagacagcta ccacatcagc tttgctggac actgtccgag
      121 gaaacaactc cttggtcaat gatctagcaa agcgtcgtct ttacgacaca gcggttgaag
      181 agtttaacgc tcgtgaccgc aggcccaagg tgaacttttc aaaagtaata agcgaggagc
      241 agacgcttat tgctacccgg gcgtatccag aattccaaat tacattttat aacacgcaaa
      301 atgccgtgca ttcgcttgca ggtggattgc gatctttaga actggaatat ctgatgatgc
      361 aaattcccta cggatcattg acttatgaca taggcgggaa ttttgcatcg catctgttca
      421 agggacgagc atatgtacac tgctgcatgc ccaacctgga cgttcgagac atcatgcggc
      481 acgaaggcca gaaagacagt attgaactat acctttctag gctagagaga ggggggaaaa
      541 cagtccccaa cttccaaaag gaagcatttg acagatacgc agaaattcct gaagacgctg
      601 tctgtcacaa tactttccag acatgcgaac atcagccgat gcagcaatca ggcagagtgt
      661 atgccattgc gctacacagc atatatgaca taccagccga tgagttcggg gcggcactct
      721 tgaggaaaaa tgtccatacg tgctatgccg ctttccactt ctccgagaac ctgcttcttg
      781 aagattcatg cgtcaatttg gacgaaatca acgcgtgttt ttcgcgcgat ggagacaagt
      841 tgaccttttc ttttgcatca gagagtactc ttaattactg tcatagttat tctaatattc
      901 ttaagtatgt gtgcaaaact tacttcccgg cctctaatag agaggtttac atgaaggagt
      961 ttttagtcac cagagttaat acctggtttt gtaagttttc tagaatagat acttttcttt
     1021 tgtacaaagg tgtggcccat aaaagtgtag atagtgagca gttttatact gcaatggaag
     1081 acgcatggca ttacaaaaag actcttgcaa tgtgcaacag cgagagaatc ctccttgagg
     1141 attcatcatc agtcaattac tggtttccca aaatgaggga tatggtcatc gtaccattat
     1201 tcgacatttc tttggagact agtaagagga cgcgcaagga agtcttagtg tccaaggatt
     1261 tcgtgtttac agtgcttaac cacattcgaa cataccaggc gaaagctctt acatacgcaa
     1321 atgttttgtc cttcgtcgaa tcgattcgat cgagggtaat cattaacggt gtgacagcga
     1381 ggtccgaatg ggatgtggac aaatctttgt tacaatcctt gtccatgacg ttttacctgc
     1441 atactaagct tgccgttcta aaggatgact tactgattag caagtttagt ctcggttcga
     1501 aaacggtgtg ccagcatgtg tgggatgaga tttcgctggc gtttgggaac gcatttccct
     1561 ccgtgaaaga gaggctcttg aacaggaaac ttatcagagt ggcaggcgac gcattagaga
     1621 tcagggtgcc tgatctatat gtgaccttcc acgacagatt agtgactgag tacaaggcct
     1681 ctgtggacat gcctgcgctt gacattagga agaagatgga agaaacggaa gtgatgtaca
     1741 atgcactttc agaattatcg gtgttaaggg agtctgacaa attcgatgtt gatgtttttt
     1801 cccagatgtg ccaatctttg gaagttgacc caatgacggc agcgaaggtt atagtcgcgg
     1861 tcatgagcaa tgagagcggt ctgactctca catttgaacg acctactgag gcgaatgttg
     1921 cgctagcttt acaggatcaa gagaaggctt cagaaggtgc attggtagtt acctcaagag
     1981 aagttgaaga accgtccatg aagggttcga tggccagagg agagttacaa ttagctggtc
     2041 ttgctggaga tcatccggaa tcgtcctatt ctaagaacga ggagatagag tctttagagc
     2101 agtttcatat ggcgacggca gattcgttaa ttcgtaagca gatgagctcg attgtgtaca
     2161 cgggtccgat taaagttcag caaatgaaaa actttatcga tagcctggta gcatcactat
     2221 ctgctgcggt gtcgaatctc gtcaagatcc tcaaagatac agctgctatt gaccttgaaa
     2281 cccgtcaaaa gtttggagtc ttggatgttg catctaggaa gtggttaatc aaaccaacgg
     2341 ccaagagtca tgcatggggt gttgttgaaa cccacgcgag gaagtatcat gtggcgcttt
     2401 tggaatatga tgagcagggt gtggtgacat gcgatgattg gagaagagta gctgttagct
     2461 ctgagtctgt tgtttattcc gacatggcga aactcagaac tctgcgcaga ctgcttcgaa
     2521 acggagaacc gcatgtcagt agcgcaaagg ttgttcttgt ggacggagtt ccgggctgtg
     2581 gaaaaaccaa agaaattctt tccagggtta attttgatga agatctaatt ttagtacctg
     2641 ggaagcaagc cgcggaaatg atcagaagac gtgcgaattc ctcagggatt attgtggcca
     2701 cgaaggacaa cgttaaaacc gttgattctt tcatgatgaa ttttgggaaa agcacacgct
     2761 gtcagttcaa gaggttattc attgatgaag ggttgatgtt gcatactggt tgtgttaatt
     2821 ttcttgtggc gatgtcattg tgcgaaattg catatgttta cggagacaca cagcagattc
     2881 catacatcaa tagagtttca ggattcccgt accccgccca ttttgccaaa ttggaagttg
     2941 acgaggtgga gacacgcaga actactctcc gttgtccagc cgatgtcaca cattatctga
     3001 acaggagata tgagggcttt gtcatgagca cttcttcggt taaaaagtct gtttcgcagg
     3061 agatggtcgg cggagccgcc gtgatcaatc cgatctcaaa acccttgcat ggcaagatct
     3121 tgacttttac ccaatcggat aaagaagctc tgctttcaag agggtattca gatgttcaca
     3181 ctgtgcatga agtgcaaggc gagacatact ctgatgtttc actagttagg ttaaccccta
     3241 caccggtctc catcattgca ggagacagcc cacatgtttt ggtcgcattg tcaaggcaca
     3301 cctgttcgct caagtactac actgttgtta tggatccttt agttagtatc attagagatc
     3361 tagagaaact tagctcgtac ttgttagata tgtataaggt cgatgcagga acacaatagc
     3421 aattacagat tgactcggtg ttcaaaggtt ccaatctttt tgttgcagcg ccaaagactg
     3481 gtgatatttc tgatatgcag ttttactatg ataagtgtct cccaggcaac agcaccatga
     3541 tgaataattt tgatgctgtt accatgaggt tgactgacat ttcattgaat gtcaaagatt
     3601 gcatattgga tatgtctaag tctgttgctg cgcctaagga tcaaatcaaa ccactaatac
     3661 ctatggtacg aacggcggca gaaatgccac gccagactgg actattggaa aatttagtgg
     3721 cgatgattaa aagaaacttt aacgcacccg agttgtctgg catcattgat attgaaaata
     3781 ctgcatcttt ggttgtagat aagttttttg atagttattt gcttaaagaa aaaagaaaac
     3841 caaataaaaa tgtttctttg ttcagtagag agtctctcaa tagatggtta gaaaagcagg
     3901 aacaggtaac aataggccag ctcgcagatt ttgattttgt ggatttgcca gcagttgatc
     3961 agtacagaca catgattaaa gcacaaccca aacaaaagtt ggacacttca atccaaacgg
     4021 agtacccggc tttgcagacg attgtgtacc attcaaaaaa gatcaatgca atattcggcc
     4081 cgttgtttag tgagcttact aggcaattac tggacagtgt tgattcgagc agatttttgt
     4141 ttttcacaag aaagacacca gcgcagattg aggatttctt cggagatctc gacagtcatg
     4201 tgccgatgga tgtcttggag ctggatatat caaaatacga caaatctcag aatgaattcc
     4261 actgtgcagt agaatacgag atctggcgaa gattgggttt cgaagacttc ttgggagaag
     4321 tttggaaaca agggcataga aagaccaccc tcaaggatta taccgcaggt ataaaaactt
     4381 gcatctggta tcaaagaaag agcggggacg tcacgacgtt cattggaaac actgtgatca
     4441 ttgctgcatg tttggcctcg atgcttccga tggagaaaat aatcaaagga gccttttgcg
     4501 gtgacgatag tctgctgtac tttccaaagg gttgtgagtt tccggatgtg caacactccg
     4561 cgaatcttat gtggaatttt gaagcaaaac tgtttaaaaa acagtatgga tacttttgcg
     4621 gaagatatgt aatacatcac gacagaggat gcattgtgta ttacgatccc ctaaagttga
     4681 tctcgaaact tggtgctaaa cacatcaagg attgggaaca cttggaggag ttcagaaggt
     4741 ctctttgtga tgttgctgtt tcgttgaaca attgtgcgta ttacacacag ttggacgacg
     4801 ctgtatggga ggttcataag accgcccctc caggttcgtt tgtttataaa agtctggtga
     4861 agtatttgtc tgataaagtt ctttttagaa gtttgtttat agatggctct agttgttaaa
     4921 ggaaaagtga atatcaatga gtttatcgac ctgacaaaaa tggagaagat cttaccgtcg
     4981 atgtttaccc ctgtaaagag tgttatgtgt tccaaagttg ataaaataat ggttcatgag
     5041 aatgagtcat tgtcaggggt gaaccttctt aaaggagtta agcttattga tagtggatac
     5101 gtctgtttag ccggtttggt cgtcacgggc gagtggaact tgcctgacaa ttgcagagga
     5161 ggtgtgagcg tgtgtctggt ggacaaaagg atggaaagag ccgacgaggc cactctcgga
     5221 tcttactaca cagcagctgc aaagaaaaga tttcagttca aggtcgttcc caattatgct
     5281 ataaccaccc aggacgcgat gaaaaacgtc tggcaagttt tagttaatat tagaaatgtg
     5341 aagatgtcag cgggtttctg tccgctttct ctggagtttg tgtcggtgtg tattgtttat
     5401 agaaataata taaaattagg tttgagagag aagattacaa acgtgagaga cggagggccc
     5461 atggaactta cagaagaagt cgttgatgag ttcatggaag atgtccctat gtcgatcagg
     5521 cttgcaaagt ttcgatctcg aaccggaaaa aagagtgatg tccgcaaagg gaaaaatagt
     5581 agtagtgatc ggtcagtgcc gaacaagaac tatagaaatg ttaaggattt tggaggaatg
     5641 agttttaaaa agaataattt aatcgatgat gattcggagg ctactgtcgc cgaatcggat
     5701 tcgttttaaa tagatcttac agtatcacta ctccatctca gttcgtgttc ttgtcattaa
     5761 ttaacggcct agggcggccg cggtcctgca acttgaggta gtcaagatgc ataataaata
     5821 acggattgtg tccgtaatca cacgtggtgc gtacgataac gcatagtgtt tttccctcca
     5881 cttaaatcga agggttgtgt cttggatcgc gcgggtcaaa tgtatatggt tcatatacat
     5941 ccgcaggcac gtaataaagc gaggggttcg aatccccccg ttacccccgg taggggccca
     6001 ggtacccgga tgtgttttcc gggttgatga gtccgtgagg acgaaacctg gctgcaggca
     6061 tgcaagcttg gcgtaatcat ggtcatagcc tagagtccgc aaaaatcacc agtctctctc
     6121 tacaaatcta tctctctcta tttttctcca gaataatgtg tgagtagttc ccagataagg
     6181 gaattagggt tcttataggg tttcgctcat gtgttgagca tataagaaac ccttagtatg
     6241 tatttgtatt tgtaaaatac ttctatcaat aaaatttcta attcctaaaa ccaaaatcca
     6301 gtgacctgca gcccggccgg gggatccact agcagattgt cgtttcccgc cttcagttta
     6361 aactatcagt gtttgacagg atatattggc gggtaaacct aagagaaaag agcgtttatt
     6421 agaataatcg gatatttaaa agggcgtgaa aaggtttatc cgttcgtcca tttgtatgtg
     6481 catgccaacc acaggagatc tcagtaaagc gctggctgaa cccccagccg gaactgaccc
     6541 cacaaggccc tagcgtttgc aatgcaccag gtcatcattg acccaggcgt gttccaccag
     6601 gccgctgcct cgcaactctt cgcaggcttc gccgacctgc tcgcgccact tcttcacgcg
     6661 ggtggaatcc gatccgcaca tgaggcggaa ggtttccagc ttgagcgggt acggctcccg
     6721 gtgcgagctg aaatagtcga acatccgtcg ggccgtcggc gacagcttgc ggtacttctc
     6781 ccatatgaat ttcgtgtagt ggtcgccagc aaacagcacg acgatttcct cgtcgatcag
     6841 gacctggcaa cgggacgttt tcttgccacg gtccaggacg cggaagcggt gcagcagcga
     6901 caccgattcc aggtgcccaa cgcggtcgga cgtgaagccc atcgccgtcg cctgtaggcg
     6961 cgacaggcat tcctcggcct tcgtgtaata ccggccattg atcgaccagc ccaggtcctg
     7021 gcaaagctcg tagaacgtga aggtgatcgg ctcgccgata ggggtgcgct tcgcgtactc
     7081 caacacctgc tgccacacca gttcgtcatc gtcggcccgc agctcgacgc cggtgtaggt
     7141 gatcttcacg tccttgttga cgtggaaaat gaccttgttt tgcagcgcct cgcgcgggat
     7201 tttcttgttg cgcgtggtga acagggcaga gcgggccgtg tcgtttggca tcgctcgcat
     7261 cgtgtccggc cacggcgcaa tatcgaacaa ggaaagctgc atttccttga tctgctgctt
     7321 cgtgtgtttc agcaacgcgg cctgcttggc ctcgctgacc tgttttgcca ggtcctcgcc
     7381 ggcggttttt cgcttcttgg tcgtcatagt tcctcgcgtg tcgatggtca tcgacttcgc
     7441 caaacctgcc gcctcctgtt cgagacgacg cgaacgctcc acggcggccg atggcgcggg
     7501 cagggcaggg ggagccagtt gcacgctgtc gcgctcgatc ttggccgtag cttgctgggc
     7561 catcgagccg acggactgga aggtttcgcg gggcgcacgc atgacggtgc ggcttgcgat
     7621 ggtttcggca tcctcggcgg aaaaccccgc gtcgatcagt tcttgcctgt atgccttccg
     7681 gtcaaacgtc cgattcattc accctccttg cgggattgcc ccgactcacg ccggggcaat
     7741 gtgcccttat tcctgatttg acccgcctgg tgccttggtg tccagataat ccaccttatc
     7801 ggcaatgaag tcggtcccgt agaccgtctg gccgtccttc tcgtacttgg tattccgaat
     7861 cttgccctgc acgaatacca gcgacccctt gcccaaatac ttgccgtggg cctcggcctg
     7921 agagccaaaa cacttgatgc ggaagaagtc ggtgcgctcc tgcttgtcgc cggcatcgtt
     7981 gcgccacatc taggtactaa aacaattcat ccagtaaaat ataatatttt attttctccc
     8041 aatcaggctt gatccccagt aagtcaaaaa atagctcgac atactgttct tccccgatat
     8101 cctccctgat cgaccggacg cagaaggcaa tgtcatacca cttgtccgcc ctgccgcttc
     8161 tcccaagatc aataaagcca cttactttgc catctttcac aaagatgttg ctgtctccca
     8221 ggtcgccgtg ggaaaagaca agttcctctt cgggcttttc cgtctttaaa aaatcataca
     8281 gctcgcgcgg atctttaaat ggagtgtctt cttcccagtt ttcgcaatcc acatcggcca
     8341 gatcgttatt cagtaagtaa tccaattcgg ctaagcggct gtctaagcta ttcgtatagg
     8401 gacaatccga tatgtcgatg gagtgaaaga gcctgatgca ctccgcatac agctcgataa
     8461 tcttttcagg gctttgttca tcttcatact cttccgagca aaggacgcca tcggcctcac
     8521 tcatgagcag attgctccag ccatcatgcc gttcaaagtg caggaccttt ggaacaggca
     8581 gctttccttc cagccatagc atcatgtcct tttcccgttc cacatcatag gtggtccctt
     8641 tataccggct gtccgtcatt tttaaatata ggttttcatt ttctcccacc agcttatata
     8701 ccttagcagg agacattcct tccgtatctt ttacgcagcg gtatttttcg atcagttttt
     8761 tcaattccgg tgatattctc attttagcca tttattattt ccttcctctt ttctacagta
     8821 tttaaagata ccccaagaag ctaattataa caagacgaac tccaattcac tgttccttgc
     8881 attctaaaac cttaaatacc agaaaacagc tttttcaaag ttgttttcaa agttggcgta
     8941 taacatagta tcgacggagc cgattttgaa accacaatta tgggtgatgc tgccaactcg
     9001 agagcgggcc gggagggttc gagaaggggg ggcacccccc ttcggcgtgc gcggtcacgc
     9061 gcacagggcg cagccctggt taaaaacaag gtttataaat attggtttaa aagcaggtta
     9121 aaagacaggt tagcggtggc cgaaaaacgg gcggaaaccc ttgcaaatgc tggattttct
     9181 gcctgtggac agcccctcaa atgtcaatag gtgcgcccct catctgtcag cactctgccc
     9241 ctcaagtgtc aaggatcgcg cccctcatct gtcagtagtc gcgcccctca agtgtcaata
     9301 ccgcagggca cttatcccca ggcttgtcca catcatctgt gggaaactcg cgtaaaatca
     9361 ggcgttttcg ccgatttgcg aggctggcca gctccacgtc gccggccgaa atcgagcctg
     9421 cccctcatct gtcaacgccg cgccgggtga gtcggcccct caagtgtcaa cgtccgcccc
     9481 tcatctgtca gtgagggcca agttttccgc gaggtatcca caacgccggc ggccggccgc
     9541 ggtgtctcgc acacggcttc gacggcgttt ctggcgcgtt tgcagggcca tagacggccg
     9601 ccagcccagc ggcgagggca accagcccgg tgagctctag tggactgatg ggctgcctgt
     9661 atcgagtggt gattttgtgc cgagctgccg gtcggggagc tgttggctgg ctggtggcag
     9721 gatatattgt ggtgtaaaca aattgacgct tagacaactt aataacacat tgcggacgtt
     9781 tttaatgtac tggggtggtt ttggtaccgg gccccccctc gaggtcgacg gtatcgataa
     9841 gcttgatatc gaattcctgc aggtcaacat ggtggagcac gacactctcg tctactccaa
     9901 gaatatcaaa gatacagtct cagaagacca aagggctatt gagacttttc aacaaagggt
     9961 aatatcggga aacctcctcg gattccattg cccagctatc tgtcacttca tcaaaaggac
    10021 agtagaaaag gaaggtggca cctacaaatg ccatcattgc gataaaggaa aggctatcgt
    10081 tcaagatgcc tctgccgaca gtggtcccaa agatggaccc ccacccacga ggagcatcgt
    10141 ggaaaaagaa gacgttccaa ccacgtcttc aaagcaagtg gattgatgtg ataacatggt
    10201 ggagcacgac actctcgtct actccaagaa tatcaaagat acagtctcag aagaccaaag
    10261 ggcaattgag acttttcaac aaagggtaat atccggaaac ctcctcggat tccattgccc
    10321 agctatctgt cactttattg tgaagatagt ggaaaaggaa ggtggctcct acaaatgcca
    10381 tcattgcgat aaaggaaagg ccatcgttga agatgcctct gccgacagtg gtcccaaaga
    10441 tggaccccca cccacgagga gcatcgtgga aaaagaagac gttccaacca cgtcttcaaa
    10501 gcaagtggat tgatgtgata tctccactga cgtaagggat gacgcacaat cccactatcc
    10561 ttcgcaagac cttcctctat ataaggaagt tcatttcatt tggagagg
//