| 质粒名称: | pMK-Prrn::RBS |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 123519 |
| 存储实验室: | Scott Lenaghan, C. Neal Stewart |
|
载体骨架基本信息 |
|
| 载体名称: | pMK-T |
| 载体出品公司: | -- |
| 载体抗性: | 卡那霉素 |
| 空载体大小: | -- |
| 完整质粒大小: | -- |
| 载体修饰: | -- |
| 载体类型: | Synthetic Biology |
| 筛选标记: | -- |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | Prrn::RBS |
| 物种来源: | -- |
| 插入大小: | -- |
| 基因突变: | -- |
| GenBank编号 | -- |
| 启动子 | -- |
| 标签/融合蛋白: | -- |
| 克隆方法: | 限制性内切酶 |
| 5' 克隆位点: | -- |
| 3' 克隆位点: | -- |
| 5' 测序引物 | -- |
| 3' 测序引物 | -- |
|
文献引用方法 |
|
| 材料和方法部分: | pMK-Prrn::RBS was a gift from Scott Lenaghan & C. Neal Stewart (Addgene plasmid # 123519 ; http://n2t.net/addgene:123519 ; RRID:Addgene_123519) |
| 参考文献部分: | MoChlo: A versatile modular cloning toolbox for chloroplast biotechnology. Occhialini A, Piatek A, Pfotenhauer AC, Frazier TP, Stewart CN, Lenaghan S. Plant Physiol. 2019 Jan 24. pii: pp.18.01220. doi: 10.1104/pp.18.01220. 10.1104/pp.18.01220 PubMed 30679266 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pMK-Prrn::RBS 2443 bp ds-DNA circular SYN 26-JUN-2019
DEFINITION MoChlo kit.
ACCESSION .
VERSION .
KEYWORDS pMK-Prrn::RBS
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 2443)
AUTHORS Occhialini A, Piatek A, Pfotenhauer AC, Frazier TP, Stewart CN,
Lenaghan S
TITLE MoChlo: A versatile modular cloning toolbox for chloroplast
biotechnology.
JOURNAL Plant Physiol. 2019 Jan 24. pii: pp.18.01220. doi:
10.1104/pp.18.01220.
PUBMED 30679266
REFERENCE 2 (bases 1 to 2443)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Jun 26, 2019 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..2443
/organism="synthetic DNA construct"
/mol_type="other DNA"
primer_bind 278..300
/label=M13/pUC Forward
/note="In lacZ gene"
primer_bind 292..309
/label=M13 Forward
/note="In lacZ gene. Also called M13-F20 or M13 (-21)
Forward"
primer_bind 293..309
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
primer_bind 320..339
/label=T7
/note="T7 promoter, forward primer"
promoter 320..338
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
CDS 343..354
/codon_start=1
/product="Factor Xa recognition and cleavage site"
/label=Factor Xa site
/translation="IEGR"
primer_bind complement(649..666)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(759..1347)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(839..858)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(1521..2315)
/codon_start=1
/product="aminoglycoside phosphotransferase"
/label=NeoR/KanR
/note="confers resistance to neomycin, kanamycin, and G418
(Geneticin(R))"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
promoter complement(2316..2420)
/gene="bla"
/label=AmpR promoter
ORIGIN
1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
121 gatagggttg agtggccgct acagggcgct cccattcgcc attcaggctg cgcaactgtt
181 gggaagggcg tttcggtgcg ggcctcttcg ctattacgcc agctggcgaa agggggatgt
241 gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg
301 acggccagtg agcgcgacgt aatacgactc actatagggc gaattgaagg aaggccgtca
361 aggccacgtg tcttgtccag agctcggtct caggagtaag aggctcgtgg gattgacgtg
421 agggggcagg gatggctata tttctgggag cgaactccgg gcgaatatga agcgcatcga
481 tacaagtgag ttgtagggag ggaaccaatg tgagaccggt acctggagca caagactggc
541 ctcatgggcc ttcctttcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca
601 ttaacatggt catagctgtt tccttgcgta ttgggcgctc tccgcttcct cgctcactga
661 ctcgctgcgc tcggtcgttc gggtaaagcc tggggtgcct aatgagcaaa aggccagcaa
721 aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct
781 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa
841 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg
901 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca
961 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa
1021 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg
1081 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg
1141 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga
1201 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc
1261 tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag
1321 attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac
1381 gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc
1441 ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag
1501 taaacttggt ctgacagtta ttagaaaaat tcatccagca gacgataaaa cgcaatacgc
1561 tggctatccg gtgccgcaat gccatacagc accagaaaac gatccgccca ttcgccgccc
1621 agttcttccg caatatcacg ggtggccagc gcaatatcct gataacgatc cgccacgccc
1681 agacggccgc aatcaataaa gccgctaaaa cggccatttt ccaccataat gttcggcagg
1741 cacgcatcac catgggtcac caccagatct tcgccatccg gcatgctcgc tttcagacgc
1801 gcaaacagct ctgccggtgc caggccctga tgttcttcat ccagatcatc ctgatccacc
1861 aggcccgctt ccatacgggt acgcgcacgt tcaatacgat gtttcgcctg atgatcaaac
1921 ggacaggtcg ccgggtccag ggtatgcaga cgacgcatgg catccgccat aatgctcact
1981 ttttctgccg gcgccagatg gctagacagc agatcctgac ccggcacttc gcccagcagc
2041 agccaatcac ggcccgcttc ggtcaccaca tccagcaccg ccgcacacgg aacaccggtg
2101 gtggccagcc agctcagacg cgccgcttca tcctgcagct cgttcagcgc accgctcaga
2161 tcggttttca caaacagcac cggacgaccc tgcgcgctca gacgaaacac cgccgcatca
2221 gagcagccaa tggtctgctg cgcccaatca tagccaaaca gacgttccac ccacgctgcc
2281 gggctacccg catgcaggcc atcctgttca atcatactct tcctttttca atattattga
2341 agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat
2401 aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cac
//
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