pLS-SV40-mP-EGFP

LOCUS       pLS-SV40-mP-EGFP              8135 bp ds-DNA     circular SYN 12-MAY-2021
DEFINITION  Lentivirus based EGFP expression vector.
ACCESSION   .
VERSION     .
KEYWORDS    pLS-SV40-mP-EGFP
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8135)
  TITLE     
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8135)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 12, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..8135
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        382..580
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     535..555
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             598..778
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    825..950
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1447..1680
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1865..1909
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2207..2324
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     primer_bind     complement(2843..2863)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        3095..3126
                     /label=minP
                     /note="minimal TATA-box promoter with low basal activity"
     regulatory      3153..3162
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     CDS             3159..3875
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3204..3225)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3465..3484)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3812..3833
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     protein_bind    3898..3931
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
     misc_feature    3987..4575
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4040..4060)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4458..4469)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(4578..4594)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4579..4595)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             5904..6084
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     rep_origin      complement(6146..6734)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6226..6245)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(6905..7765)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7528..7547
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7766..7870)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(7945..7964)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag cgcgttttgc ctgtactggg
      601 tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg
      661 cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt
      721 gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt
      781 ggcgcccgaa cagggacttg aaagcgaaag ggaaaccaga ggagctctct cgacgcagga
      841 ctcggcttgc tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg agtacgccaa
      901 aaattttgac tagcggaggc tagaaggaga gagatgggtg cgagagcgtc agtattaagc
      961 gggggagaat tagatcgcga tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat
     1021 ataaattaaa acatatagta tgggcaagca gggagctaga acgattcgca gttaatcctg
     1081 gcctgttaga aacatcagaa ggctgtagac aaatactggg acagctacaa ccatcccttc
     1141 agacaggatc agaagaactt agatcattat ataatacagt agcaaccctc tattgtgtgc
     1201 atcaaaggat agagataaaa gacaccaagg aagctttaga caagatagag gaagagcaaa
     1261 acaaaagtaa gaccaccgca cagcaagcgg ccggccgctg atcttcagac ctggaggagg
     1321 agatatgagg gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc
     1381 attaggagta gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt
     1441 gggaatagga gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc
     1501 gtcaatgacg ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa
     1561 caatttgctg agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat
     1621 caagcagctc caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct
     1681 ggggatttgg ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag
     1741 ttggagtaat aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag
     1801 agaaattaac aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca
     1861 agaaaagaat gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt
     1921 taacataaca aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt
     1981 aggtttaaga atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc
     2041 accattatcg tttcagaccc acctcccaac cccgagggga cccgacaggc ccgaaggaat
     2101 agaagaagaa ggtggagaga gagacagaga cagatccatt cgattagtga acggatcggc
     2161 actgcgtgcg ccaattctgc agacaaatgg cagtattcat ccacaatttt aaaagaaaag
     2221 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
     2281 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
     2341 acagcagaga tccagtttgg ttagtaccgg gcccggtgct ttgctctgag ccagcccacc
     2401 agtttggaat gactcctttt tatgacttga attttcaagt ataaagtcta gtgctaaatt
     2461 taatttgaac aactgtatag tttttgctgg ttgggggaag gaaaaaaaat ggtggcagtg
     2521 tttttttcag aattagaagt gaaatgaaaa cttgttgtgt gtgaggattt ctaatgacat
     2581 gtggtggttg catactgagt gaagccggtg agcattctgc catgtcaccc cctcgtgctc
     2641 agtaatgtac tttacagaaa tcctaaactc aaaagattga tataaaccat gcttcttgtg
     2701 tatatccggt ctcttctctg ggtagtctca ctcagcctgc atttctgcca gggcccgctc
     2761 tagagcgcag caccatggcc tgaaataacc tctgaaagag gaacttggtt aggtaccttc
     2821 tgaggcggaa agaaccagct gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc
     2881 tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac caggtgtgga
     2941 aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca
     3001 accatagtcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat
     3061 tctccgcccc atggctgtct agacctgcag gcactagagg gtatataatg gaagctcgac
     3121 ttccagcttg gcaatccggt actgtaccgg tcgccaccat ggtgagcaag ggcgaggagc
     3181 tgttcaccgg ggtggtgccc atcctggtcg agctggacgg cgacgtaaac ggccacaagt
     3241 tcagcgtgtc cggcgagggc gagggcgatg ccacctacgg caagctgacc ctgaagttca
     3301 tctgcaccac cggcaagctg cccgtgccct ggcccaccct cgtgaccacc ctgacctacg
     3361 gcgtgcagtg cttcagccgc taccccgacc acatgaagca gcacgacttc ttcaagtccg
     3421 ccatgcccga aggctacgtc caggagcgca ccatcttctt caaggacgac ggcaactaca
     3481 agacccgcgc cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc gagctgaagg
     3541 gcatcgactt caaggaggac ggcaacatcc tggggcacaa gctggagtac aactacaaca
     3601 gccacaacgt ctatatcatg gccgacaagc agaagaacgg catcaaggtg aacttcaaga
     3661 tccgccacaa catcgaggac ggcagcgtgc agctcgccga ccactaccag cagaacaccc
     3721 ccatcggcga cggccccgtg ctgctgcccg acaaccacta cctgagcacc cagtccgccc
     3781 tgagcaaaga ccccaacgag aagcgcgatc acatggtcct gctggagttc gtgaccgccg
     3841 ccgggatcac tctcggcatg gacgagctgt acaagtagga attcgtcgag ggacctaata
     3901 acttcgtata gcatacatta tacgaagtta tacatgttta agggttccgg ttccactagg
     3961 tacaattcga tatcaagctt atcgataatc aacctctgga ttacaaaatt tgtgaaagat
     4021 tgactggtat tcttaactat gttgctcctt ttacgctatg tggatacgct gctttaatgc
     4081 ctttgtatca tgctattgct tcccgtatgg ctttcatttt ctcctccttg tataaatcct
     4141 ggttgctgtc tctttatgag gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca
     4201 ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc caccacctgt cagctccttt
     4261 ccgggacttt cgctttcccc ctccctattg ccacggcgga actcatcgcc gcctgccttg
     4321 cccgctgctg gacaggggct cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga
     4381 aatcatcgtc ctttccttgg ctgctcgcct gtgttgccac ctggattctg cgcgggacgt
     4441 ccttctgcta cgtcccttcg gccctcaatc cagcggacct tccttcccgc ggcctgctgc
     4501 cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt
     4561 gggccgcctc cccgcatcga taccgtcgac ctcgatcgag acctagaaaa acatggagca
     4621 atcacaagta gcaatacagc agctaccaat gctgattgtg cctggctaga agcacaagag
     4681 gaggaggagg tgggttttcc agtcacacct caggtaccaa gcatggggta aagtactgtt
     4741 ctcatcacat catatcaagg ttatatacca tcaatattgc cacagatgtt acttagcctt
     4801 ttaatatttc tctaatttag tgtatatgca atgatagttc tctgatttct gagattgagt
     4861 ttctcatgtg taatgattat ttagagtttc tctttcatct gttcaaattt ttgtctagtt
     4921 ttatttttta ctgatttgta agacttcttt ttataatctg catattacaa ttctctttac
     4981 tggggtgttg caaatatttt ctgtcattct atggcctgac ttttcttaat ggttttttaa
     5041 ttttaaaaat aagtcttaat attcatgcaa tctaattaac aatcttttct ttgtggttag
     5101 gactttgagt cataagaaat ttttctctac actgaagtca tgatggcatg cttctatatt
     5161 attttctaaa agatttaaag ttttgccttc tccatttaga cttataattc actggaattt
     5221 ttttgtgtgt atggtatgac atatgggttc ccttttattt tttacatata aatatatttc
     5281 cctgtttttc taaaaaagaa aaagatcatc attttcccat tgtaaaatgc catatttttt
     5341 tcataggtca cttacatata tcaatgggtc tgtttctgag ctctactcta ttttatcagc
     5401 ctcactgtct atccccacac atctcatgct ttgctctaaa tcttgatatt tagtggaaca
     5461 ttctttccca ttttgttcta caagaatatt tttgttattg tcttttgggc ttctatatac
     5521 attttagaat gaggttggca agttctgtag atcttagcca ctttttaaaa gaaaaggggg
     5581 gactggaagg gctaattcac tcccaacgaa gacaagatat ccttgatctg tggatctacc
     5641 acacacaagg ctacttccct gattggcaga actacacacc agggccaggg atcagatatc
     5701 cactgacctt tggatggtgc tacaagctag taccagttga gcaagagaag gtagaagaag
     5761 ccaatgaagg agagaacacc cgcttgttac accctgtgag cctgcatggg atggatgacc
     5821 cggagagaga agtattagag tggaggtttg acagccgcct agcatttcat cacatggccc
     5881 gagagctgca tccggactgt actgggtctc tctggttaga ccagatctga gcctgggagc
     5941 tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc
     6001 aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt
     6061 agtcagtgtg gaaaatctct agcagcatgt gagcaaaagg ccagcaaaag gccaggaacc
     6121 gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca
     6181 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt
     6241 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc
     6301 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc
     6361 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc
     6421 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact
     6481 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg
     6541 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta
     6601 tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca
     6661 aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa
     6721 aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg
     6781 aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc
     6841 ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg
     6901 acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat
     6961 ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg
     7021 gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa
     7081 taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca
     7141 tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc
     7201 gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt
     7261 cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa
     7321 aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat
     7381 cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct
     7441 tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga
     7501 gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag
     7561 tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga
     7621 gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca
     7681 ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg
     7741 cgacacggaa atgttgaata ctcatactct tcctttttca atattattga agcatttatc
     7801 agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag
     7861 gggttccgcg cacatttccc cgaaaagtgc cacctgacgt cgacggatcg ggagatctcc
     7921 cgatccccta tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta
     7981 tctgctccct gcttgtgtgt tggaggtcgc tgagtagtgc gcgagcaaaa tttaagctac
     8041 aacaaggcaa ggcttgaccg acaattgcat gaagaatctg cttagggtta ggcgttttgc
     8101 gctgcttcgc gatgtacggg ccagatatac gcgtt
//