| 质粒名称: | ABE8.8-m |
|---|---|
| 出品公司: | addgene |
| 目录编号: | 136294 |
| 存储实验室: | Nicole Gaudelli |
|
载体骨架基本信息 |
|
| 载体名称: | pBR322 |
| 载体出品公司: | 无 |
| 空载体大小: | 3400 bp |
| 完整质粒大小: | 8166 bp |
| 载体类型: | 哺乳细胞表达 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | NEB Stable |
| 拷贝数: | 高拷贝 |
|
插入基因信息 |
|
| 插入基因名称: | ABE8.8-m |
| 插入大小: | 5300 bp |
| 基因突变: | D10A in S. pyogenes Cas9, TadA mutations described in manuscript |
| 标签/融合蛋白: | C-terminal BPNLS (C terminal on insert) |
| 克隆方法: | Ligation Independent Cloning |
| 5’测序引物 | T7 |
| 3’测序引物 | M13R |
|
文献引用方法 |
|
| 材料和方法部分: | ABE8.8-m was a gift from Nicole Gaudelli (Addgene plasmid # 136294 ; http://n2t.net/addgene:136294 ; RRID:Addgene_136294) |
| 参考文献部分: |
Directed evolution of adenine base editors with increased activity and therapeutic application. Gaudelli NM, Lam DK, Rees HA, Sola-Esteves NM, Barrera LA, Born DA, Edwards A, Gehrke JM, Lee SJ, Liquori AJ, Murray R, Packer MS, Rinaldi C, Slaymaker IM, Yen J, Young LE, Ciaramella G. Nat Biotechnol. 2020 Apr 13. pii: 10.1038/s41587-020-0491-6. doi: 10.1038/s41587-020-0491-6. 10.1038/s41587-020-0491-6 PubMed 32284586
|
买家导航
ABE8.8-m载体质粒基本信息
ABE8.8-m质粒图谱载体图谱和ABE8.8-m载体序列质粒序列多克隆位点信息
ABE8.8-m质粒载体简介
ABE8.8-m质粒序列载体序列
LOCUS ABE8.8-m 8166 bp ds-DNA circular SYN 12-MAY-2021
DEFINITION expresses ABE8.8-m in mammalian cells.
ACCESSION .
VERSION .
KEYWORDS ABE8.8-m
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 8166)
AUTHORS Gaudelli NM, Lam DK, Rees HA, Sola-Esteves NM, Barrera LA, Born DA,
Edwards A, Gehrke JM, Lee SJ, Liquori AJ, Murray R, Packer MS,
Rinaldi C, Slaymaker IM, Yen J, Young LE, Ciaramella G
TITLE Directed evolution of adenine base editors with increased activity
and therapeutic application.
JOURNAL Nat Biotechnol. 2020 Apr 13. pii: 10.1038/s41587-020-0491-6. doi:
10.1038/s41587-020-0491-6.
PUBMED 32284586
REFERENCE 2 (bases 1 to 8166)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58
http://www.biofeng.com
FEATURES Location/Qualifiers
source 1..8166
/organism="synthetic DNA construct"
/mol_type="other DNA"
enhancer 111..490
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 491..694
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind 645..665
/label=CMV-F
/note="Human CMV immediate early promoter, forward primer"
primer_bind 736..755
/label=T7
/note="T7 promoter, forward primer"
promoter 736..754
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
CDS 771..1268
/codon_start=1
/product="DNA adenosine deaminase evolved from the E. coli
tRNA adenosine deaminase TadA (Gaudelli et al., 2017)"
/label=TadA*(7.10)
/translation="SEVEFSHEYWMRHALTLAKRARDEREVPVGAVLVLNNRVIGEGWN
RAIGLHDPTAHAEIMALRQGGLVMQNYRLIDATLYVTFEPCVMCAGAMIHSRIGRVVFG
VRNAKTGAAGSLMDVLHHPGMNHRVEITEGILADECAALLCRFFRMPRRVFNAQKKAQS
STD"
CDS 1365..5465
/codon_start=1
/product="nickase mutant of the Cas9 endonuclease from the
Streptococcus pyogenes Type II CRISPR/Cas system"
/label=Cas9(D10A)
/note="generates RNA-guided single strand nicks in DNA due
to the D10A mutation in the RuvC catalytic domain"
/translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
ATLIHQSITGLYETRIDLSQLGGD"
CDS 5511..5531
/codon_start=1
/product="nuclear localization signal of SV40 (simian virus
40) large T antigen"
/label=SV40 NLS
/translation="PKKKRKV"
CDS 5540..5557
/codon_start=1
/product="6xHis affinity tag"
/label=6xHis
/translation="HHHHHH"
primer_bind complement(5580..5597)
/label=BGH-rev
/note="Bovine growth hormone terminator, reverse primer.
Also called BGH reverse"
polyA_signal 5586..5810
/label=bGH poly(A) signal
/note="bovine growth hormone polyadenylation signal"
primer_bind complement(5881..5897)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(5881..5897)
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind complement(5894..5916)
/label=M13/pUC Reverse
/note="In lacZ gene"
protein_bind 5905..5921
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(5929..5959)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 5974..5995
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
primer_bind complement(6112..6129)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(6283..6871)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(6363..6382)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(7042..7902)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 7665..7684
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(7903..8007)
/gene="bla"
/label=AmpR promoter
primer_bind complement(8086..8105)
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
ORIGIN
1 caaaatttaa gctacaacaa ggcaaggctt gaccgacaat tgcatgaaga atctgcttag
61 ggttaggcgt tttgcgctgc ttcgcgatgt acgggccaga tatacgcgtt gacattgatt
121 attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc catatatgga
181 gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca acgacccccg
241 cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga ctttccattg
301 acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc aagtgtatca
361 tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc
421 ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat tagtcatcgc
481 tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc
541 acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa
601 tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag
661 gcgtgtacgg tgggaggtct atataagcag agctggttta gtgaaccgtc agatccgcta
721 gagatccgcg gccgctaata cgactcacta tagggagagc cgccaccatg tccgaagtcg
781 agttttccca tgagtactgg atgagacacg cattgactct cgcaaagagg gctcgagatg
841 aacgcgaggt gcccgtgggg gcagtactcg tgctcaacaa tcgcgtaatc ggcgaaggtt
901 ggaatagggc aatcggactc cacgacccca ctgcacatgc ggaaatcatg gcccttcgac
961 agggagggct tgtgatgcag aattatcgac ttatcgatgc gacgctgtac gtcacgtttg
1021 aaccttgcgt aatgtgcgcg ggagctatga ttcactcccg cattggacga gttgtattcg
1081 gtgttcgcaa cgccaagacg ggtgccgcag gttcactgat ggacgtgctg catcatccag
1141 gcatgaacca ccgggtagaa atcacagaag gcatattggc ggacgaatgt gcggcgctgt
1201 tgtgtcgttt ttttcgcatg cccaggcggg tctttaacgc ccagaaaaaa gcacaatcct
1261 ctactgactc tggtggttct tctggtggtt ctagcggcag cgagactccc gggacctcag
1321 agtccgccac acccgaaagt tctggtggtt cttctggtgg ttctgacaag aagtacagca
1381 tcggcctggc catcggcacc aactctgtgg gctgggccgt gatcaccgac gagtacaagg
1441 tgcccagcaa gaaattcaag gtgctgggca acaccgaccg gcacagcatc aagaagaacc
1501 tgatcggagc cctgctgttc gacagcggcg aaacagccga ggccacccgg ctgaagagaa
1561 ccgccagaag aagatacacc agacggaaga accggatctg ctatctgcaa gagatcttca
1621 gcaacgagat ggccaaggtg gacgacagct tcttccacag actggaagag tccttcctgg
1681 tggaagagga taagaagcac gagcggcacc ccatcttcgg caacatcgtg gacgaggtgg
1741 cctaccacga gaagtacccc accatctacc acctgagaaa gaaactggtg gacagcaccg
1801 acaaggccga cctgcggctg atctatctgg ccctggccca catgatcaag ttccggggcc
1861 acttcctgat cgagggcgac ctgaaccccg acaacagcga cgtggacaag ctgttcatcc
1921 agctggtgca gacctacaac cagctgttcg aggaaaaccc catcaacgcc agcggcgtgg
1981 acgccaaggc catcctgtct gccagactga gcaagagcag acggctggaa aatctgatcg
2041 cccagctgcc cggcgagaag aagaatggcc tgttcggaaa cctgattgcc ctgagcctgg
2101 gcctgacccc caacttcaag agcaacttcg acctggccga ggatgccaaa ctgcagctga
2161 gcaaggacac ctacgacgac gacctggaca acctgctggc ccagatcggc gaccagtacg
2221 ccgacctgtt tctggccgcc aagaacctgt ccgacgccat cctgctgagc gacatcctga
2281 gagtgaacac cgagatcacc aaggcccccc tgagcgcctc tatgatcaag agatacgacg
2341 agcaccacca ggacctgacc ctgctgaaag ctctcgtgcg gcagcagctg cctgagaagt
2401 acaaagagat tttcttcgac cagagcaaga acggctacgc cggctacatt gacggcggag
2461 ccagccagga agagttctac aagttcatca agcccatcct ggaaaagatg gacggcaccg
2521 aggaactgct cgtgaagctg aacagagagg acctgctgcg gaagcagcgg accttcgaca
2581 acggcagcat cccccaccag atccacctgg gagagctgca cgccattctg cggcggcagg
2641 aagattttta cccattcctg aaggacaacc gggaaaagat cgagaagatc ctgaccttcc
2701 gcatccccta ctacgtgggc cctctggcca ggggaaacag cagattcgcc tggatgacca
2761 gaaagagcga ggaaaccatc accccctgga acttcgagga agtggtggac aagggcgctt
2821 ccgcccagag cttcatcgag cggatgacca acttcgataa gaacctgccc aacgagaagg
2881 tgctgcccaa gcacagcctg ctgtacgagt acttcaccgt gtataacgag ctgaccaaag
2941 tgaaatacgt gaccgaggga atgagaaagc ccgccttcct gagcggcgag cagaaaaagg
3001 ccatcgtgga cctgctgttc aagaccaacc ggaaagtgac cgtgaagcag ctgaaagagg
3061 actacttcaa gaaaatcgag tgcttcgact ccgtggaaat ctccggcgtg gaagatcggt
3121 tcaacgcctc cctgggcaca taccacgatc tgctgaaaat tatcaaggac aaggacttcc
3181 tggacaatga ggaaaacgag gacattctgg aagatatcgt gctgaccctg acactgtttg
3241 aggacagaga gatgatcgag gaacggctga aaacctatgc ccacctgttc gacgacaaag
3301 tgatgaagca gctgaagcgg cggagataca ccggctgggg caggctgagc cggaagctga
3361 tcaacggcat ccgggacaag cagtccggca agacaatcct ggatttcctg aagtccgacg
3421 gcttcgccaa cagaaacttc atgcagctga tccacgacga cagcctgacc tttaaagagg
3481 acatccagaa agcccaggtg tccggccagg gcgatagcct gcacgagcac attgccaatc
3541 tggccggcag ccccgccatt aagaagggca tcctgcagac agtgaaggtg gtggacgagc
3601 tcgtgaaagt gatgggccgg cacaagcccg agaacatcgt gatcgaaatg gccagagaga
3661 accagaccac ccagaaggga cagaagaaca gccgcgagag aatgaagcgg atcgaagagg
3721 gcatcaaaga gctgggcagc cagatcctga aagaacaccc cgtggaaaac acccagctgc
3781 agaacgagaa gctgtacctg tactacctgc agaatgggcg ggatatgtac gtggaccagg
3841 aactggacat caaccggctg tccgactacg atgtggacca tatcgtgcct cagagctttc
3901 tgaaggacga ctccatcgac aacaaggtgc tgaccagaag cgacaagaac cggggcaaga
3961 gcgacaacgt gccctccgaa gaggtcgtga agaagatgaa gaactactgg cggcagctgc
4021 tgaacgccaa gctgattacc cagagaaagt tcgacaatct gaccaaggcc gagagaggcg
4081 gcctgagcga actggataag gccggcttca tcaagagaca gctggtggaa acccggcaga
4141 tcacaaagca cgtggcacag atcctggact cccggatgaa cactaagtac gacgagaatg
4201 acaagctgat ccgggaagtg aaagtgatca ccctgaagtc caagctggtg tccgatttcc
4261 ggaaggattt ccagttttac aaagtgcgcg agatcaacaa ctaccaccac gcccacgacg
4321 cctacctgaa cgccgtcgtg ggaaccgccc tgatcaaaaa gtaccctaag ctggaaagcg
4381 agttcgtgta cggcgactac aaggtgtacg acgtgcggaa gatgatcgcc aagagcgagc
4441 aggaaatcgg caaggctacc gccaagtact tcttctacag caacatcatg aactttttca
4501 agaccgagat taccctggcc aacggcgaga tccggaagcg gcctctgatc gagacaaacg
4561 gcgaaaccgg ggagatcgtg tgggataagg gccgggattt tgccaccgtg cggaaagtgc
4621 tgagcatgcc ccaagtgaat atcgtgaaaa agaccgaggt gcagacaggc ggcttcagca
4681 aagagtctat cctgcccaag aggaacagcg ataagctgat cgccagaaag aaggactggg
4741 accctaagaa gtacggcggc ttcgacagcc ccaccgtggc ctattctgtg ctggtggtgg
4801 ccaaagtgga aaagggcaag tccaagaaac tgaagagtgt gaaagagctg ctggggatca
4861 ccatcatgga aagaagcagc ttcgagaaga atcccatcga ctttctggaa gccaagggct
4921 acaaagaagt gaaaaaggac ctgatcatca agctgcctaa gtactccctg ttcgagctgg
4981 aaaacggccg gaagagaatg ctggcctctg ccggcgaact gcagaaggga aacgaactgg
5041 ccctgccctc caaatatgtg aacttcctgt acctggccag ccactatgag aagctgaagg
5101 gctcccccga ggataatgag cagaaacagc tgtttgtgga acagcacaag cactacctgg
5161 acgagatcat cgagcagatc agcgagttct ccaagagagt gatcctggcc gacgctaatc
5221 tggacaaagt gctgtccgcc tacaacaagc accgggataa gcccatcaga gagcaggccg
5281 agaatatcat ccacctgttt accctgacca atctgggagc ccctgccgcc ttcaagtact
5341 ttgacaccac catcgaccgg aagaggtaca ccagcaccaa agaggtgctg gacgccaccc
5401 tgatccacca gagcatcacc ggcctgtacg agacacggat cgacctgtct cagctgggag
5461 gtgacgaggg agctgataag cgcaccgccg atggttccga gttcgaaagc cccaagaaga
5521 agaggaaagt ctaaccggtc atcatcacca tcaccattga gtttaaaccc gctgatcagc
5581 ctcgactgtg ccttctagtt gccagccatc tgttgtttgc ccctcccccg tgccttcctt
5641 gaccctggaa ggtgccactc ccactgtcct ttcctaataa aatgaggaaa ttgcatcgca
5701 ttgtctgagt aggtgtcatt ctattctggg gggtggggtg gggcaggaca gcaaggggga
5761 ggattgggaa gacaatagca ggcatgctgg ggatgcggtg ggctctatgg cttctgaggc
5821 ggaaagaacc agctggggct cgataccgtc gacctctagc tagagcttgg cgtaatcatg
5881 gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc
5941 cggaagcata aagtgtaaag cctagggtgc ctaatgagtg agctaactca cattaattgc
6001 gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat
6061 cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac
6121 tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt
6181 aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca
6241 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc
6301 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact
6361 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct
6421 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag
6481 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca
6541 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa
6601 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc
6661 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag
6721 aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg
6781 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca
6841 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc
6901 tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag
6961 gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata
7021 tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat
7081 ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg
7141 ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc
7201 tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc
7261 aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc
7321 gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc
7381 gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc
7441 ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa
7501 gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat
7561 gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata
7621 gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca
7681 tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag
7741 gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc
7801 agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc
7861 aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata
7921 ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta
7981 gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtcga
8041 cggatcggga gatcgatctc ccgatcccct agggtcgact ctcagtacaa tctgctctga
8101 tgccgcatag ttaagccagt atctgctccc tgcttgtgtg ttggaggtcg ctgagtagtg
8161 cgcgag
//
