| 质粒名称: | pGBW-m4046663 |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 153857 |
| 存储实验室: | Ginkgo Bioworks, Benjie Chen |
|
载体骨架基本信息 |
|
| 载体名称: | AmpR; 2u URA3 |
| 载体出品公司: | -- |
| 载体抗性: | 氨苄青霉素 |
| 空载体大小: | 4078 bp |
| 完整质粒大小: | -- |
| 载体修饰: | -- |
| 载体类型: | 酵母表达 |
| 筛选标记: | URA3 |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | SARS-CoV-2 nsp3 |
| 物种来源: | Severe acute respiratory syndrome coronavirus 2 |
| 插入大小: | 5834 bp |
| 基因突变: | wt |
| GenBank编号 | -- |
| 启动子 | pGAL1 |
| 标签/融合蛋白: | -- |
| 克隆方法: | -- |
| 5' 克隆位点: | -- |
| 3' 克隆位点: | -- |
| 5' 测序引物 | -- |
| 3' 测序引物 | -- |
|
文献引用方法 |
|
| 材料和方法部分: | pGBW-m4046663 was a gift from Ginkgo Bioworks & Benjie Chen (Addgene plasmid # 153857 ; http://n2t.net/addgene:153857 ; RRID:Addgene_153857) |
买家导航
pGBW-m4046663载体 pGBWm4046663质粒基本信息
pGBW-m4046663质粒图谱 pGBWm4046663载体图谱和pGBW-m4046663载体序列 pGBWm4046663质粒序列多克隆位点信息
pGBW-m4046663质粒 pGBWm4046663载体简介
pGBW-m4046663质粒序列 pGBWm4046663载体序列
LOCUS pGBW-m4046663 10605 bp ds-DNA circular SYN 12-MAY-2021
DEFINITION Yeast expression plasmid for SARS-CoV-2 nsp3.
ACCESSION .
VERSION .
KEYWORDS pGBW-m4046663
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 10605)
TITLE SARS-CoV-2 protein Yeast expression constructs, tagged, for protein
expression and/or purification
REFERENCE 2 (bases 1 to 10605)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..10605
/organism="synthetic DNA construct"
/mol_type="other DNA"
promoter 25..466
/gene="S. cerevisiae GAL1"
/label=GAL1 promoter
/note="inducible promoter, regulated by Gal4"
protein_bind 25..142
/label=UAS
/bound_moiety="Gal4"
/note="upstream activating sequence mediating
Gal4-dependent induction"
primer_bind 437..460
/label=GAL1
/note="S. cerevisiae GAL1 promoter, forward primer"
primer_bind complement(6360..6378)
/label=CYC1
/note="CYC1 transcription termination signal, reverse
primer"
CDS 6729..7532
/codon_start=1
/gene="S. cerevisiae URA3"
/product="orotidine-5'-phosphate decarboxylase, required
for uracil biosynthesis"
/label=URA3
/note="yeast auxotrophic marker, counterselectable with
5-fluoroorotic acid (5-FOA)"
/translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
rep_origin 7615..8597
/label=2u ori
/note="yeast 2u plasmid origin of replication"
terminator 8675..8704
/label=T3Te terminator
/note="phage T3 early transcription terminator"
rep_origin 8726..9313
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
terminator 9325..9352
/label=T7Te terminator
/note="phage T7 early transcription terminator"
CDS complement(9376..9567)
/codon_start=1
/gene="rop"
/product="Rop protein, which maintains plasmids at low copy
number"
/label=rop
/translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
DELYRSCLARFGDDGENL"
CDS complement(9568..10428)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESLRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLATIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGSLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 10191..10210
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(10429..10531)
/label=cat promoter
/note="promoter of the E. coli cat gene encoding
chloramphenicol acetyltransferase"
terminator 10532..10563
/label=tonB terminator
/note="bidirectional E. coli tonB-P14 transcription
terminator"
ORIGIN
1 gcggccgctt gctatattga agtacggatt agaagccgcc gagcgggcga cagccctccg
61 acggaagact ctcctccgtg cgtcctcgtc ttcaccggtc gcgttcctga aacgcagatg
121 tgcctcgcgc cgcactgctc cgaacaataa agattctaca atactagctt ttatggttat
181 gaagaggaaa aattggcagt aacctggccc cacaaacctt caaattaacg aatcaaatta
241 acaaccatag gatgataatg cgattagttt tttagcctta tttctggggt aattaatcag
301 cgaagcgatg atttttgatc tattaacaga tatataaatg gaaaagctgc ataaccactt
361 taactaatac tttcaacatt ttcagtttgt attacttctt attcaaatgt cataaaagta
421 tcaacaaaaa attgttaata tacctctata ctttaacgtc aaggagaaaa aactataatg
481 gcccccacta aggtcacatt cggggatgac accgtgatag aggttcaggg ttacaaatcc
541 gttaacatca cttttgaatt agatgaaaga attgacaagg tattgaatga aaagtgctca
601 gcatataccg ttgaactggg tacggaggtc aacgaatttg cttgtgtcgt tgcggatgct
661 gttatcaaaa ctttgcaacc tgtgtctgaa cttctaaccc cattaggaat tgacttggat
721 gaatggtcta tggctactta ctatctattc gatgaaagtg gtgagttcaa gttggcttcc
781 cacatgtact gttcgttcta cccaccagac gaagacgaag aagaaggcga ctgtgaagaa
841 gaagagtttg aaccaagcac ccaatatgaa tacggtacgg aagatgatta ccaaggtaag
901 ccattggaat ttggtgccac ttctgctgca ttgcaacctg aagaagagca agaagaagat
961 tggttagacg atgactctca gcaaacagtc ggtcaacaag acggttccga ggataatcaa
1021 actaccacca ttcaaacaat cgttgaggta caaccacaac ttgaaatgga attgactcca
1081 gtcgttcaga ccatagaagt taactcgttc tcaggttact tgaagctaac tgataatgtt
1141 tatattaaaa acgctgacat cgtcgaagaa gcaaagaaag ttaagcctac cgtcgtggtt
1201 aacgccgcta acgtttattt gaagcatggt ggcggtgtag ctggtgcttt aaacaaagcc
1261 actaataacg ctatgcaagt cgaatctgac gattacattg ctaccaatgg tccattgaag
1321 gtgggaggct cttgcgtctt aagtggacac aacttggcga aacattgtct ccacgttgtt
1381 ggtcccaatg ttaacaaggg tgaagacatt caactgttga agtccgccta cgaaaatttc
1441 aaccaacatg aggttctctt ggcaccatta ctgagtgctg gtatcttcgg cgctgaccca
1501 attcactctt taagggtctg tgtggatact gtaagaacaa acgtctactt ggccgttttc
1561 gataaaaacc tttatgacaa gttagtctct tcctttttgg aaatgaagtc agaaaagcaa
1621 gttgaacaga aaatcgcgga aatccctaag gaagaagtaa agccgtttat aaccgaatcg
1681 aagccctctg ttgagcaaag aaagcaagat gacaaaaaga ttaaagcttg tgttgaggaa
1741 gtaacaacta cattagagga aactaagttt ctcacggaaa atctattgtt gtacatagat
1801 atcaatggta acttgcaccc agactccgct actttggtca gcgatatcga cattacattc
1861 ttgaagaagg acgctccata tattgtgggt gacgttgtcc aagaaggtgt tttaactgct
1921 gttgtcatcc caactaagaa ggcagggggt accacggaga tgctagctaa ggccttgcgt
1981 aaagtcccga ctgataatta tattaccacc taccctggtc agggtctaaa cggttacacg
2041 gttgaagaag ctaaaactgt tctaaaaaag tgtaagtctg ctttttacat attgccatca
2101 attatttcca acgaaaagca agaaatattg ggtaccgtat cttggaattt gagagaaatg
2161 ttagctcatg ctgaggaaac tagaaagttg atgcctgtct gcgttgaaac taaagctatc
2221 gtcagcacga tccaaagaaa atacaagggt attaaaatcc aagaaggcgt tgtggactat
2281 ggagcccgtt tctatttcta cacttcgaag accaccgtcg cttcgttgat caacaccttg
2341 aacgatctca acgagacact tgttactatg ccactcggct atgtaactca cggtcttaac
2401 ttggaagaag ctgcgagata catgcgctct ctgaaagttc ctgccacagt ctccgtatct
2461 tcaccagacg ctgttacagc ctataatgga tacttgactt cttcatcaaa aaccccagaa
2521 gaacatttca tcgaaactat ctctctagct ggtagttaca aggattggtc ttactccggt
2581 caatccacac aactaggtat agagtttctg aagagaggtg acaagagtgt ttactacacc
2641 agcaatccaa cgacctttca tttagatggt gaagtcatta cgttcgataa cttgaagact
2701 ttgttgagtt tgcgggaagt ccgaactatc aaggttttca ctaccgttga taacattaac
2761 ctccacaccc aagttgttga tatgtctatg acttatggtc aacagttcgg tccgacatat
2821 ttggatggtg ctgacgttac taaaattaag ccacacaact cccacgaagg gaagacattc
2881 tacgtgcttc caaatgacga cactttacga gtcgaagcct ttgagtacta ccataccacg
2941 gacccatctt tccttggtag atacatgtcc gctctgaatc acactaaaaa gtggaaatac
3001 ccacaagtta acggtttgac ttctattaag tgggccgata ataactgtta cttggctacc
3061 gccttattga ctttgcaaca gatagaactc aagtttaatc ctcccgctct acaagatgca
3121 tattatagag ctagagcagg cgaagcggct aatttctgtg ctttaatttt ggcttactgt
3181 aacaagaccg taggtgaatt gggggatgtc agagagacta tgtcctatct tttccagcac
3241 gcaaacttgg attcatgtaa gagagtgtta aatgtagtct gcaagacctg cggtcaacaa
3301 caaactacct taaagggtgt tgaagccgtt atgtacatgg gtacactttc ttacgaacaa
3361 tttaagaagg gtgtccaaat cccatgtact tgtggtaagc aagccactaa atacttggtc
3421 cagcaagaaa gccctttcgt tatgatgagc gccccaccag ctcaatacga gctgaagcat
3481 ggaactttca cctgtgcttc tgaatatacc ggtaactacc aatgcggtca ctacaagcat
3541 attactagca aagaaacttt gtactgtatc gatggtgcct tgttgactaa gtcctcagaa
3601 tataagggcc caattactga cgtgttttac aaagagaata gttacactac cacaattaag
3661 ccagtaacat acaaactgga tggtgttgtg tgtaccgaga tcgaccctaa gttagataac
3721 tattacaaga aggacaactc ttactttacc gaacaaccga tcgacctagt cccaaatcag
3781 ccatatccaa atgcttcttt tgataacttc aagttcgttt gtgataacat aaaattcgcc
3841 gacgatttaa accaattgac cggttacaaa aagccggctt caagagagtt gaaagtcact
3901 ttcttcccag atttaaatgg cgatgtcgtt gctattgact ataagcacta cactccatct
3961 ttcaagaagg gcgccaagtt gctacacaag ccaattgtct ggcacgttaa caacgctacc
4021 aacaaggcta cgtataagcc taatacctgg tgtatcagat gtctatggtc caccaaacca
4081 gttgaaacga gtaactcttt tgacgttctg aaatcagaag atgcccaagg gatggataac
4141 ttagcatgtg aagacttgaa acctgtttca gaagaagttg tggaaaatcc aactattcag
4201 aaggacgtgt tggaatgcaa cgtgaaaaca actgaagtag ttggtgatat aatcctgaag
4261 ccagctaaca actcactgaa gattaccgaa gaagtcggtc acaccgactt gatggcagcc
4321 tacgttgata actcttccct cacgatcaaa aagccaaatg aattgtctcg tgtactcggg
4381 ctaaagaccc tagctaccca tggtctagcg gctgtcaact ccgtgccgtg ggacaccatc
4441 gctaactacg ctaagccttt tttgaataag gtcgttagca ccacgacaaa tatagttact
4501 agatgtttga acagagtgtg taccaattac atgccttatt tctttactct attgctgcaa
4561 ttgtgcactt tcactcgctc tacaaactcc agaattaaag catctatgcc aaccaccatc
4621 gccaagaaca cggttaagtc agtaggtaag ttctgcttag aggcaagctt taactatttg
4681 aagtctccaa acttctctaa gttgatcaat attattatct ggtttttact tttgagcgtt
4741 tgtttgggtt ccttgatcta ctcaacggct gctttgggtg tcttgatgtc taacttgggt
4801 atgccgagtt actgtactgg ttatagagaa ggttatttga actcaacaaa cgttactatt
4861 gctacgtact gcaccggttc cattccatgt tctgtttgtt tatccggttt agactcgttg
4921 gacacttacc catctttaga aaccatccaa attaccattt cctctttcaa gtgggatttg
4981 acagctttcg gtcttgtcgc tgaatggttt ttggcctata tattgtttac tcgtttcttc
5041 tacgtcttag gcttggccgc tatcatgcag ttgtttttct cttattttgc agtccatttt
5101 atctcaaatt cgtggttgat gtggttgatt attaatctcg ttcaaatggc tccaatttcg
5161 gcaatggtta ggatgtacat tttctttgct tcattctatt atgtatggaa atcttacgtc
5221 catgtggttg atggttgtaa ctcctcgacc tgtatgatgt gttacaagag aaatagagcg
5281 actagggttg agtgcacaac tatcgttaac ggtgtcagac gttctttcta cgtatacgct
5341 aacggtggta agggcttttg taaactccat aactggaatt gtgttaattg tgataccttt
5401 tgcgctggtt ctacctttat tagtgatgaa gtagccagag acttaagtct ccaattcaaa
5461 cggccaatca acccaactga tcaatcctct tatattgtcg atagtgtcac cgttaagaac
5521 ggtagtatcc acttatactt cgataaggcc ggtcaaaaga catacgaaag acactcattg
5581 tcccactttg ttaatttaga caatttgaga gctaataata ctaaggggtc tttacccatc
5641 aacgtcatag tcttcgatgg taagtctaaa tgtgaagaat cctccgctaa gtctgcgtca
5701 gtttactact ctcaattgat gtgccaaccc atcctacttc tggatcaagc tttggtatct
5761 gacgttggtg actctgccga ggttgctgtt aagatgttcg atgcttacgt taacacattt
5821 tcatcaactt tcaacgtgcc aatggaaaag ctgaagactt tggtggctac cgctgaagca
5881 gaattggcta agaatgtttc tttggataac gttttaagta cgttcatttc cgcagctcgt
5941 cagggtttcg tcgactctga cgtagaaacc aaagacgtcg tcgaatgttt gaagctctct
6001 catcaatccg atattgaagt cactggtgac tcctgtaata actacatgct gacgtataac
6061 aaagtggaaa acatgactcc tagagatttg ggagcgtgca tcgactgttc tgctagacac
6121 ataaacgctc aagttgcaaa atcccataat attgctttga tctggaacgt taaagatttc
6181 atgtcactat ccgagcaact taggaaacaa atccggtctg cggcaaagaa aaataacctt
6241 ccattcaaat taacatgtgc aacaactaga caagttgtga acgttgttac tactaagatc
6301 gctctgaagg gtggttaaac aggccccttt tcctttgtcg atatcatgta attagttatg
6361 tcacgcttac attcacgccc tcctcccaca tccgctctaa ccgaaaagga aggagttaga
6421 caacctgaag tctaggtccc tatttatttt ttttaatagt tatgttagta ttaagaacgt
6481 tatttatatt tcaaattttt cttttttttc tgtacaaagc ggccgccgaa ccactagtgc
6541 actgcagtac acggtttcct tgaaattttt ttgattcggt aatctccgaa cagaaggaag
6601 aacgaaggaa ggagcacaga cttagattgg tatatatacg catatgtagt gttgaagaaa
6661 catgaaattg cccagtattc ttaacccaac tgcacagaac aaaaacgtgc aggaaacgaa
6721 gataaatcat gtcgaaagct acatataagg aacgtgctgc tactcatcct agtcctgttg
6781 ctgccaagct atttaatatc atgcacgaaa agcaaacaaa cttgtgtgct tcattggatg
6841 ttcgtaccac caaggaatta ctggagttag ttgaagcatt aggtcccaaa atttgtttac
6901 taaaaacaca tgtggatatc ttgactgatt tttccatgga gggcacagtt aagccgctaa
6961 aggcattatc cgccaagtac aattttttac tcttcgagga cagaaaattt gctgacattg
7021 gtaatacagt caaattgcag tactctgcgg gtgtatacag aatagcagaa tgggcagaca
7081 ttacgaatgc acacggtgtg gtgggcccag gtattgttag cggtttgaag caggcggcag
7141 aagaagtaac aaaggaacct agaggccttt tgatgttagc agaattgtca tgcaagggct
7201 ccctatctac tggagaatat actaagggta ctgttgacat tgcgaagagc gacaaagatt
7261 ttgttatcgg ctttattgct caaagagaca tgggtggaag agatgaaggt tacgattggt
7321 tgattatgac acccggtgtg ggtttagatg acaagggaga tgcattgggt caacagtata
7381 gaaccgtgga tgatgtggtt tctacaggat ctgacattat tattgttgga agaggactat
7441 ttgcaaaggg aagggatgct aaggtagagg gtgaacgtta cagaaaagca ggctgggaag
7501 catatttgag aagatgcggc cagcaaaact aaaaaactgt attataagta aatgcatgta
7561 tactaaactc acaaattaga gcttcaattt aattatatca gttattaccc gagtaacgaa
7621 gcatctgtgc ttcattttgt agaacaaaaa tgcaacgcga gagcgctaat ttttcaaaca
7681 aagaatctga gctgcatttt tacagaacag aaatgcaacg cgaaagcgct attttaccaa
7741 cgaagaatct gtgcttcatt tttgtaaaac aaaaatgcaa cgcgagagcg ctaatttttc
7801 aaacaaagaa tctgagctgc atttttacag aacagaaatg caacgcgaga gcgctatttt
7861 accaacaaag aatctatact tcttttttgt tctacaaaaa tgcatcccga gagcgctatt
7921 tttctaacaa agcatcttag attacttttt ttctcctttg tgcgctctat aatgcagtct
7981 cttgataact ttttgcactg taggtccgtt aaggttagaa gaaggctact ttggtgtcta
8041 ttttctcttc cataaaaaaa gcctgactcc acttcccgcg tttactgatt actagcgaag
8101 ctgcgggtgc attttttcaa gataaaggca tccccgatta tattctatac cgatgtggat
8161 tgcgcatact ttgtgaacag aaagtgatag cgttgatgat tcttcattgg tcagaaaatt
8221 atgaacggtt tcttctattt tgtctctata tactacgtat aggaaatgtt tacattttcg
8281 tattgttttc gattcactct atgaatagtt cttactacaa tttttttgtc taaagagtaa
8341 tactagagat aaacataaaa aatgtagagg tcgagtttag atgcaagttc aaggagcgaa
8401 aggtggatgg gtaggttata tagggatata gcacagagat atatagcaaa gagatacttt
8461 tgagcaatgt ttgtggaagc ggtattcgca atattttagt agctcgttac agtccggtgc
8521 gtttttggtt ttttgaaagt gcgtcatcag agcgcttttg gttttcaaaa gcgctctgaa
8581 gttcctatac tttctagcta gagaatagga acttcccgag cggccgcgtg ttacaagctt
8641 gactccagcg taactggact gcaatcaact cactggctca ccttcacggg tgggcctttc
8701 ttcggtagaa aatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct
8761 tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa
8821 ctctttttcc gaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt
8881 gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct
8941 gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga
9001 ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac
9061 acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg
9121 agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt
9181 cggaacagga gagcgcacga gggagcttcc aggggtaaac gcctggtatc tttatagtcc
9241 tgtcgggttt cgccacctct gacttgagca tcgatttttg tgatgctcgt caggggggcg
9301 gagcctatgg aaaaacgcca gcaacgcaga aaggcccacc cgaaggtgag ccaggtgatt
9361 acatttgggc cctcatcaga ggttttcacc gtcatcaccg aaacgcgcga ggcagctgcg
9421 gtaaagctca tcagcgtggt cgtgaagcga ttcacagatg tctgcctgtt catccgcgtc
9481 cagctcgttg agtttctcca gaagcgttaa tgtctggctt ctgataaagc gggccatgtt
9541 aagggcggtt ttttcctgtt tggtcactta ccaatgctta atcagtgagg cacctatctc
9601 agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac
9661 gatgcgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc
9721 accggctcca gatttatcag caataaacca gccagccggg agggccgagc gcagaagtga
9781 tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag
9841 tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc
9901 acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac
9961 atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgccag
10021 aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac
10081 tgtcatgcca tccgtgagat gcttttctgt gactggtgag tactcaacca agtcattctg
10141 agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc
10201 gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgtaaact
10261 ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg
10321 atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa
10381 tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcattt tagcttcctt
10441 agctcctgaa aatctcgata actcaaaaaa tacgcccggt agtgatctta tttcattatg
10501 gtgaaagttg gaacctctta cgtgccgatc aagtcaaaag cctccggtcg gaggcttttg
10561 actttctgct atggaggtca ggtatgattt aaatggtcag ttaga
//
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pGBW-m4046663质粒 pGBWm4046663载体应用举例
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