| 质粒名称: | pSLCAR-CD19-28z |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 135991 |
| 存储实验室: | Scott McComb |
|
载体骨架基本信息 |
|
| 载体名称: | pSL |
| 载体出品公司: | Heavily modified from LentiCRISPR v1 backbone |
| 载体抗性: | 氨苄青霉素 |
| 载体修饰: | 使用BpiI单锅限制性连接将CAR的抗原结合域从CD19交换到所需的特异性 |
| 载体类型: | 哺乳动物表达，慢病毒 |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | FMC63-28-3z-P2A-EGFP,CD19(FMC63)-CAR, CD28 costim., CD3z stim domains, P2A-GFP marker,Contains: FMC63-scFV, Hinge domain, CD28-transmembrane domain, CD28-ITD, CD3z-ITD |
| 物种来源: | H. sapiens (human) |
| 启动子 | EF1a-short |
| 标签/融合蛋白: | -- |
| 克隆方法: | 限制性内切酶 |
| 5' 克隆位点: | BpiI（在克隆过程中被破坏） |
| 3' 克隆位点: | BpiI（在克隆过程中被破坏） |
| 5' 测序引物 | CGGGTTTGCCGCCA |
| 3' 测序引物 | caggagccTGCTCCTCTTACTcc |
|
文献引用方法 |
|
| 材料和方法部分: | pSLCAR-CD19-28z was a gift from Scott McComb (Addgene plasmid # 135991 ; http://n2t.net/addgene:135991 ; RRID:Addgene_135991) |
| 参考文献部分: | A High-Throughput Method for Characterizing Novel Chimeric Antigen Receptors in Jurkat Cells. Bloemberg D, Nguyen T, MacLean S, Zafer A, Gadoury C, Gurnani K, Chattopadhyay A, Ash J, Lippens J, Harcus D, Page M, Fortin A, Pon RA, Gilbert R, Marcil A, Weeratna RD, McComb S. Mol Ther Methods Clin Dev. 2020 Jan 31;16:238-254. doi: 10.1016/j.omtm.2020.01.012. eCollection 2020 Mar 13. 10.1016/j.omtm.2020.01.012 PubMed 32083149 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pSLCAR-CD19-28z 8866 bp ds-DNA circular SYN 12-MAY-2021
DEFINITION Modular CD28-CD3z CAR backbone, For Transient Expression or
Lentiviral Production.
ACCESSION .
VERSION .
KEYWORDS pSLCAR-CD19-28z
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 8866)
AUTHORS Bloemberg D, Nguyen T, MacLean S, Zafer A, Gadoury C, Gurnani K,
Chattopadhyay A, Ash J, Lippens J, Harcus D, Page M, Fortin A, Pon
RA, Gilbert R, Marcil A, Weeratna RD, McComb S
TITLE A High-Throughput Method for Characterizing Novel Chimeric Antigen
Receptors in Jurkat Cells.
JOURNAL Mol Ther Methods Clin Dev. 2020 Jan 31;16:238-254. doi:
10.1016/j.omtm.2020.01.012. eCollection 2020 Mar 13.
PUBMED 32083149
REFERENCE 2 (bases 1 to 8866)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..8866
/organism="synthetic DNA construct"
/mol_type="other DNA"
primer_bind complement(52..71)
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
enhancer 243..622
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 624..822
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind 777..797
/label=CMV-F
/note="Human CMV immediate early promoter, forward primer"
LTR 840..1020
/label=5' LTR (truncated)
/note="truncated 5' long terminal repeat (LTR) from HIV-1"
misc_feature 1067..1192
/label=HIV-1 Psi
/note="packaging signal of human immunodeficiency virus
type 1"
misc_feature 1685..1918
/label=RRE
/note="The Rev response element (RRE) of HIV-1 allows for
Rev-dependent mRNA export from the nucleus to the
cytoplasm."
CDS 2103..2147
/codon_start=1
/product="antigenic peptide corresponding to amino acids
655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
et al., 2013)"
/label=gp41 peptide
/note="recognized by the 2H10 single-chain llama nanobody"
/translation="KNEQELLELDKWASL"
misc_feature 2370..2487
/label=cPPT/CTS
/note="central polypurine tract and central termination
sequence of HIV-1"
promoter 2513..2724
/label=EF-1-alpha core promoter
/note="core promoter for human elongation factor
EF-1-alpha"
CDS 2813..2878
/codon_start=1
/product="three tandem FLAG(R) epitope tags, followed by an
enterokinase cleavage site"
/label=3xFLAG
/translation="DYKDHDGDYKDHDIDYKDDDDK"
CDS 4343..4399
/codon_start=1
/product="2A peptide from porcine teschovirus-1
polyprotein"
/label=P2A
/note="Eukaryotic ribosomes fail to insert a peptide bond
between the Gly and Pro residues, yielding separate
polypeptides."
/translation="ATNFSLLKQAGDVEENPGP"
CDS 4400..5116
/codon_start=1
/product="the original enhanced GFP (Yang et al., 1996)"
/label=EGFP
/note="mammalian codon-optimized"
/translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
FVTAAGITLGMDELYK"
CDS 4400..5113
/codon_start=1
/product="enhanced GFP"
/label=EGFP
/translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
FVTAAGITLGMDELYK"
primer_bind complement(4442..4463)
/label=EGFP-N
/note="EGFP, reverse primer"
primer_bind complement(4703..4722)
/label=EXFP-R
/note="For distinguishing EGFP variants, reverse primer"
primer_bind 5050..5071
/label=EGFP-C
/note="EGFP, forward primer"
misc_feature 5131..5719
/label=WPRE
/note="woodchuck hepatitis virus posttranscriptional
regulatory element"
primer_bind complement(5184..5204)
/label=WPRE-R
/note="WPRE, reverse primer"
CDS complement(5602..5613)
/codon_start=1
/product="Factor Xa recognition and cleavage site"
/label=Factor Xa site
/translation="IEGR"
LTR 5791..6024
/label=3' LTR (Delta-U3)
/note="self-inactivating 3' long terminal repeat (LTR) from
HIV-1"
polyA_signal 6102..6223
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(6139..6158)
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind 6193..6212
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
rep_origin 6263..6398
/label=SV40 ori
/note="SV40 origin of replication"
primer_bind 6325..6344
/label=SV40pro-F
/note="SV40 promoter/origin, forward primer"
primer_bind complement(6418..6437)
/label=T7
/note="T7 promoter, forward primer"
promoter complement(6419..6437)
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
primer_bind complement(6447..6464)
/label=M13 Forward
/note="In lacZ gene. Also called M13-F20 or M13 (-21)
Forward"
primer_bind complement(6447..6463)
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(6456..6478)
/label=M13/pUC Forward
/note="In lacZ gene"
rep_origin 6605..7060
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(6692..6711)
/label=F1ori-R
/note="F1 origin, reverse primer"
primer_bind 6902..6923
/label=F1ori-F
/note="F1 origin, forward primer"
promoter 7086..7190
/gene="bla"
/label=AmpR promoter
CDS 7191..8051
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind complement(7409..7428)
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
rep_origin 8222..8810
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 8711..8730
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
ORIGIN
1 catgtgtcga cggatcggga gatctcccga tcccctatgg tgcactctca gtacaatctg
61 ctctgatgcc gcatagttaa gccagtatct gctccctgct tgtgtgttgg aggtcgctga
121 gtagtgcgcg agcaaaattt aagctacaac aaggcaaggc ttgaccgaca attgcatgaa
181 gaatctgctt agggttaggc gttttgcgct gcttcgcgat gtacgggcca gatatacgcg
241 ttgacattga ttattgacta gttattaata gtaatcaatt acggggtcat tagttcatag
301 cccatatatg gagttccgcg ttacataact tacggtaaat ggcccgcctg gctgaccgcc
361 caacgacccc cgcccattga cgtcaataat gacgtatgtt cccatagtaa cgccaatagg
421 gactttccat tgacgtcaat gggtggagta tttacggtaa actgcccact tggcagtaca
481 tcaagtgtat catatgccaa gtacgccccc tattgacgtc aatgacggta aatggcccgc
541 ctggcattat gcccagtaca tgaccttatg ggactttcct acttggcagt acatctacgt
601 attagtcatc gctattacca tggtgatgcg gttttggcag tacatcaatg ggcgtggata
661 gcggtttgac tcacggggat ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt
721 ttggcaccaa aatcaacggg actttccaaa atgtcgtaac aactccgccc cattgacgca
781 aatgggcggt aggcgtgtac ggtgggaggt ctatataagc agcgcgtttt gcctgtactg
841 ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac
901 tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt
961 gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca
1021 gtggcgcccg aacagggact tgaaagcgaa agggaaacca gaggagctct ctcgacgcag
1081 gactcggctt gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagtacgcc
1141 aaaaattttg actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa
1201 gcgggggaga attagatcgc gatgggaaaa aattcggtta aggccagggg gaaagaaaaa
1261 atataaatta aaacatatag tatgggcaag cagggagcta gaacgattcg cagttaatcc
1321 tggcctgtta gaaacatcag aaggctgtag acaaatactg ggacagctac aaccatccct
1381 tcagacagga tcagaagaac ttagatcatt atataataca gtagcaaccc tctattgtgt
1441 gcatcaaagg atagagataa aagacaccaa ggaagcttta gacaagatag aggaagagca
1501 aaacaaaagt aagaccaccg cacagcaagc ggccgctgat cttcagacct ggaggaggag
1561 atatgaggga caattggaga agtgaattat ataaatataa agtagtaaaa attgaaccat
1621 taggagtagc acccaccaag gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg
1681 gaataggagc tttgttcctt gggttcttgg gagcagcagg aagcactatg ggcgcagcgt
1741 caatgacgct gacggtacag gccagacaat tattgtctgg tatagtgcag cagcagaaca
1801 atttgctgag ggctattgag gcgcaacagc atctgttgca actcacagtc tggggcatca
1861 agcagctcca ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa cagctcctgg
1921 ggatttgggg ttgctctgga aaactcattt gcaccactgc tgtgccttgg aatgctagtt
1981 ggagtaataa atctctggaa cagatttgga atcacacgac ctggatggag tgggacagag
2041 aaattaacaa ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag
2101 aaaagaatga acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta
2161 acataacaaa ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag
2221 gtttaagaat agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac
2281 cattatcgtt tcagacccac ctcccaaccc cgaggggacc ccgggtttat tacagggaca
2341 gcagagatcc actttggcgc cggctcgagt tttaaaagaa aaggggggat tggggggtac
2401 agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa agaattacaa
2461 aaacaaatta caaaaattca aaatttttcg agtggctccg gtgcccgtca gtgggcagag
2521 cgcacatcgc ccacagtccc cgagaagttg gggggagggg tcggcaattg aaccggtgcc
2581 tagagaaggt ggcgcggggt aaactgggaa agtgatgtcg tgtactggct ccgccttttt
2641 cccgagggtg ggggagaacc gtatataagt gcagtagtcg ccgtgaacgt tctttttcgc
2701 aacgggtttg ccgccagaac acaggtgtcg tgacgcggga tccgccacca tgctcaggct
2761 gctcttggct ctcaacttat tcccttcaat tcaagtaaca ggagggtctt cggactacaa
2821 ggatcatgac ggagactata aggatcacga tattgattac aaagatgacg acgacaaaga
2881 catccagatg acacagacta catcctccct gtctgcctct ctgggagaca gagtcaccat
2941 cagttgcagg gcaagtcagg acatctctaa gtatttgaat tggtatcagc agaaaccaga
3001 tggaactgtt aaactcctga tctaccatac atcaagatta cactcaggag tcccatcaag
3061 gttcagtggc agtgggtctg gaacagatta ttctctcacc attagcaacc tggagcaaga
3121 agatattgcc acttactttt gccaacaggg taatacgctt ccgtacacgt tcggaggggg
3181 gactaagttg gaaataacag gctccacctc tggatccggc aagcccggat ctggcgaggg
3241 atccaccaag ggcgaggtga aactgcagga gtcaggacct ggcctggtgg cgccctcaca
3301 gagcctgtcc gtcacatgca ctgtctcagg ggtctcatta cccgactatg gtgtaagctg
3361 gattcgccag cctccacgaa agggtctgga gtggctggga gtaatatggg gtagtgaaac
3421 cacatactat aattcagctc tcaaatccag actgaccatc atcaaggaca actccaagag
3481 ccaagttttc ttaaaaatga acagtctgca aactgatgac acagccattt actactgtgc
3541 caaacattat tactacggtg gtagctatgc tatggactac tggggtcaag gaacctcagt
3601 caccgtctcc tcagcggccg caggtaccac cacaacgccc gctcctcggc caccgacgcc
3661 agcgccaact attgcgagtc agcctctcag tctgcgacct gaggcttgtc gaccagcagc
3721 cggaggcgca gtgcacacga gggggctgga cttcgcctgt gatagaagac ctccttctaa
3781 gcccttttgg gtgctggtgg tggttggtgg agtcctggct tgctatagct tgctagtaac
3841 agtggccttt attattttct gggtgaggag taagaggagc aggctcctgc acagtgacta
3901 catgaacatg actcccaggc ggcccggacc cacccgcaag cattaccagc cctatgcccc
3961 accacgcgac ttcgcagcct atcgctccgc tagcctgaga gtgaagttca gcaggagcgc
4021 agacgccccc gcgtaccagc agggccagaa ccagctctat aacgagctca atctaggacg
4081 aagagaggag tacgatgttt tggacaagag acgtggccgg gaccctgaga tggggggaaa
4141 gccgcagaga aggaagaacc ctcaggaagg cctgtacaat gaactgcaga aagataagat
4201 ggcggaggcc tacagtgaga ttgggatgaa aggcgagcgc cggaggggca aggggcacga
4261 tggcctttac cagggtctca gtacagccac caaggacacc tacgacgccc ttcacatgca
4321 ggccctgccc cctcgcgcta gcgccacgaa cttctctctg ttaaagcaag caggcgacgt
4381 ggaagaaaac cccggtcccg tgagcaaggg cgaggagctg ttcaccgggg tggtgcccat
4441 cctggtcgag ctggacggcg acgtaaacgg ccacaagttc agcgtgtccg gcgagggcga
4501 gggcgatgcc acctacggca agctgaccct gaagttcatc tgcaccaccg gcaagctgcc
4561 cgtgccctgg cccaccctcg tgaccaccct gacctacggc gtgcagtgct tcagccgcta
4621 ccccgaccac atgaagcagc acgacttctt caagtccgcc atgcccgaag gctacgtcca
4681 ggagcgcacc atcttcttca aggacgacgg caactacaag acccgcgccg aggtgaagtt
4741 cgagggcgac accctggtga accgcatcga gctgaagggc atcgacttca aggaggacgg
4801 caacatcctg gggcacaagc tggagtacaa ctacaacagc cacaacgtct atatcatggc
4861 cgacaagcag aagaacggca tcaaggtgaa cttcaagatc cgccacaaca tcgaggacgg
4921 cagcgtgcag ctcgccgacc actaccagca gaacaccccc atcggcgacg gccccgtgct
4981 gctgcccgac aaccactacc tgagcaccca gtccgccctg agcaaagacc ccaacgagaa
5041 gcgcgatcac atggtcctgc tggagttcgt gaccgccgcc gggatcactc tcggcatgga
5101 cgagctgtac aagtaacgcg ttaagtcgac aatcaacctc tggattacaa aatttgtgaa
5161 agattgactg gtattcttaa ctatgttgct ccttttacgc tatgtggata cgctgcttta
5221 atgcctttgt atcatgctat tgcttcccgt atggctttca ttttctcctc cttgtataaa
5281 tcctggttgc tgtctcttta tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg
5341 tgcactgtgt ttgctgacgc aacccccact ggttggggca ttgccaccac ctgtcagctc
5401 ctttccggga ctttcgcttt ccccctccct attgccacgg cggaactcat cgccgcctgc
5461 cttgcccgct gctggacagg ggctcggctg ttgggcactg acaattccgt ggtgttgtcg
5521 gggaaatcat cgtcctttcc ttggctgctc gcctgtgttg ccacctggat tctgcgcggg
5581 acgtccttct gctacgtccc ttcggccctc aatccagcgg accttccttc ccgcggcctg
5641 ctgccggctc tgcggcctct tccgcgtctt tgccttcgcc ctcagacgag tcggatctcc
5701 ctttgggccg cctccccgcg tcgactttaa gaccaatgac ttacaaggca gctgtagatc
5761 ttagccactt tttaaaagaa aaggggggac tggaagggct aattcactcc caacgaagat
5821 aagatctgct ttttgcttgt actgggtctc tctggttaga ccagatctga gcctgggagc
5881 tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc
5941 aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt
6001 agtcagtgtg gaaaatctct agcagtacgt atagtagttc atgtcatctt attattcagt
6061 atttataact tgcaaagaaa tgaatatcag agagtgagag gaacttgttt attgcagctt
6121 ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac
6181 tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tggctctagc
6241 tatcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc
6301 gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct cggcctctga
6361 gctattccag aagtagtgag gaggcttttt tggaggccta gggacgtacc caattcgccc
6421 tatagtgagt cgtattacgc gcgctcactg gccgtcgttt tacaacgtcg tgactgggaa
6481 aaccctggcg ttacccaact taatcgcctt gcagcacatc cccctttcgc cagctggcgt
6541 aatagcgaag aggcccgcac cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa
6601 tgggacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg
6661 accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc
6721 gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga
6781 tttagtgctt tacggcacct cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt
6841 gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat
6901 agtggactct tgttccaaac tggaacaaca ctcaacccta tctcggtcta ttcttttgat
6961 ttataaggga ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa
7021 tttaacgcga attttaacaa aatattaacg cttacaattt aggtggcact tttcggggaa
7081 atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca
7141 tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc
7201 aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc
7261 acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt
7321 acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt
7381 ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtattgacg
7441 ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact
7501 caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg
7561 ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga
7621 aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg
7681 aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa
7741 tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac
7801 aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc
7861 cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca
7921 ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga
7981 gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta
8041 agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc
8101 atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc
8161 cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt
8221 cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac
8281 cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct
8341 tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact
8401 tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg
8461 ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata
8521 aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga
8581 cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag
8641 ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg
8701 agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac
8761 ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca
8821 acgcggcctt tttacggttc ctggcctttt gctggccttt tgctca
//
载体图谱质粒图谱pdf版和相关资料下载
载体质粒应用举例
- 上一篇：PU6::klp-12_sgRNA
- 下一篇：CGBE1 (pRZ3885)
