pENTR2B

价格:1000元

联系方式:I47-825O-882O

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载体基本信息

出品公司: Invitrogen
载体名称: pENTR2B
质粒类型: Gateway载体;入门载体
高拷贝/低拷贝: 高拷贝
启动子:
克隆方法: 限制性内切酶;多克隆位点
载体大小: 2718 bp
5' 测序引物及序列: --
3' 测序引物及序列: --
载体标签:
载体抗性: 卡那霉素
真核筛选标记:
备注:
pENTR2B载体本身含有CCDb致死基因,能够使得普通的DH5alpha等细菌死亡,必须使用DB3.1感受态细胞进行质粒扩增;

构建Gateway系统入门克隆时需要使用ccdB敏感的 OmniMAX2 感受态细胞。
 
产品目录号: --
病毒/非病毒: 非病毒

载体质粒图谱和多克隆位点信息

pENTR2B载体图谱



pENTR2B多克隆位点

载体简介

载体序列

LOCUS       pENTR2B	2718 bp 	DNA	SYN
DEFINITION  pENTR2B
ACCESSION   
KEYWORDS    
SOURCE      
  ORGANISM  other sequences; artificial sequences; vectors.
FEATURES             Location/Qualifiers
     source          1..2718
                     /organism="pENTR2B"
                     /mol_type="other DNA"
     terminator      106..149
                     /label="rrnB_T1_terminator"
     terminator      281..308
                     /label="rrnB_T2_terminator"
     misc_feature    complement(358..441)
                     /label="attL2"
     misc_feature    complement(358..457)
                     /label="attL1"
     misc_feature    613..912
                     /label="ccdB"
     misc_feature    947..1046
                     /label="attL2"
     gene            1169..1978
                     /label="KanR2"
                     /gene="KanR2"
     CDS             1169..1978
                     /label="ORF frame 2"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGK
                     PDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVD
                     ASDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRV
                     GIADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF*"
     rep_origin      2052..2671
                     /label="pBR322_origin"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGK
                     PDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVD
                     ASDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRV
                     GIADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF*"
ORIGIN
    1 CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCTAGCA TGGATCTCGG
   61 GGACGTCTAA CTACTAAGCG AGAGTAGGGA ACTGCCAGGC ATCAAATAAA ACGAAAGGCT
  121 CAGTCGGAAG ACTGGGCCTT TCGTTTTATC TGTTGTTTGT CGGTGAACGC TCTCCTGAGT
  181 AGGACAAATC CGCCGGGAGC GGATTTGAAC GTTGTGAAGC AACGGCCCGG AGGGTGGCGG
  241 GCAGGACGCC CGCCATAAAC TGCCAGGCAT CAAACTAAGC AGAAGGCCAT CCTGACGGAT
  301 GGCCTTTTTG CGTTTCTACA AACTCTTCCT GTTAGTTAGT TACTTAAGCT CGGGCCCCAA
  361 ATAATGATTT TATTTTGACT GATAGTGACC TGTTCGTTGC AACAAATTGA TAAGCAATGC
  421 TTTTTTATAA TGCCAACTTT GTACAAAAAA GCAGGCTGGC GCCGGAACCA ATTCAGTCGA
  481 CTGGATCCGG TACCGAATTC GCTTACTAAA AGCCAGATAA CAGTATGCGT ATTTGCGCGC
  541 TGATTTTTGC GGTATAAGAA TATATACTGA TATGTATACC CGAAGTATGT CAAAAAGAGG
  601 TGTGCTTCTA GAATGCAGTT TAAGGTTTAC ACCTATAAAA GAGAGAGCCG TTATCGTCTG
  661 TTTGTGGATG TACAGAGTGA TATTATTGAC ACGCCCGGGC GACGGATGGT GATCCCCCTG
  721 GCCAGTGCAC GTCTGCTGTC AGATAAAGTC TCCCGTGAAC TTTACCCGGT GGTGCATATC
  781 GGGGATGAAA GCTGGCGCAT GATGACCACC GATATGGCCA GTGTGCCGGT CTCCGTTATC
  841 GGGGAAGAAG TGGCTGATCT CAGCCACCGC GAAAATGACA TCAAAAACGC CATTAACCTG
  901 ATGTTCTGGG GAATATAGAA TTCGCGGCCG CACTCGAGAT ATCTAGACCC AGCTTTCTTG
  961 TACAAAGTTG GCATTATAAG AAAGCATTGC TTATCAATTT GTTGCAACGA ACAGGTCACT
 1021 ATCAGTCAAA ATAAAATCAT TATTTGCCAT CCAGCTGCAG CTCTGGCCCG TGTCTCAAAA
 1081 TCTCTGATGT TACATTGCAC AAGATAAAAA TATATCATCA TGAACAATAA AACTGTCTGC
 1141 TTACATAAAC AGTAATACAA GGGGTGTTAT GAGCCATATT CAACGGGAAA CGTCGAGGCC
 1201 GCGATTAAAT TCCAACATGG ATGCTGATTT ATATGGGTAT AAATGGGCTC GCGATAATGT
 1261 CGGGCAATCA GGTGCGACAA TCTATCGCTT GTATGGGAAG CCCGATGCGC CAGAGTTGTT
 1321 TCTGAAACAT GGCAAAGGTA GCGTTGCCAA TGATGTTACA GATGAGATGG TCAGACTAAA
 1381 CTGGCTGACG GAATTTATGC CTCTTCCGAC CATCAAGCAT TTTATCCGTA CTCCTGATGA
 1441 TGCATGGTTA CTCACCACTG CGATCCCCGG AAAAACAGCA TTCCAGGTAT TAGAAGAATA
 1501 TCCTGATTCA GGTGAAAATA TTGTTGATGC GCTGGCAGTG TTCCTGCGCC GGTTGCATTC
 1561 GATTCCTGTT TGTAATTGTC CTTTTAACAG CGATCGCGTA TTTCGTCTCG CTCAGGCGCA
 1621 ATCACGAATG AATAACGGTT TGGTTGATGC GAGTGATTTT GATGACGAGC GTAATGGCTG
 1681 GCCTGTTGAA CAAGTCTGGA AAGAAATGCA TAAACTTTTG CCATTCTCAC CGGATTCAGT
 1741 CGTCACTCAT GGTGATTTCT CACTTGATAA CCTTATTTTT GACGAGGGGA AATTAATAGG
 1801 TTGTATTGAT GTTGGACGAG TCGGAATCGC AGACCGATAC CAGGATCTTG CCATCCTATG
 1861 GAACTGCCTC GGTGAGTTTT CTCCTTCATT ACAGAAACGG CTTTTTCAAA AATATGGTAT
 1921 TGATAATCCT GATATGAATA AATTGCAGTT TCATTTGATG CTCGATGAGT TTTTCTAATC
 1981 AGAATTGGTT AATTGGTTGT AACATTATTC AGATTGGGCC CCGTTCCACT GAGCGTCAGA
 2041 CCCCGTAGAA AAGATCAAAG GATCTTCTTG AGATCCTTTT TTTCTGCGCG TAATCTGCTG
 2101 CTTGCAAACA AAAAAACCAC CGCTACCAGC GGTGGTTTGT TTGCCGGATC AAGAGCTACC
 2161 AACTCTTTTT CCGAAGGTAA CTGGCTTCAG CAGAGCGCAG ATACCAAATA CTGTTCTTCT
 2221 AGTGTAGCCG TAGTTAGGCC ACCACTTCAA GAACTCTGTA GCACCGCCTA CATACCTCGC
 2281 TCTGCTAATC CTGTTACCAG TGGCTGCTGC CAGTGGCGAT AAGTCGTGTC TTACCGGGTT
 2341 GGACTCAAGA CGATAGTTAC CGGATAAGGC GCAGCGGTCG GGCTGAACGG GGGGTTCGTG
 2401 CACACAGCCC AGCTTGGAGC GAACGACCTA CACCGAACTG AGATACCTAC AGCGTGAGCT
 2461 ATGAGAAAGC GCCACGCTTC CCGAAGGGAG AAAGGCGGAC AGGTATCCGG TAAGCGGCAG
 2521 GGTCGGAACA GGAGAGCGCA CGAGGGAGCT TCCAGGGGGA AACGCCTGGT ATCTTTATAG
 2581 TCCTGTCGGG TTTCGCCACC TCTGACTTGA GCGTCGATTT TTGTGATGCT CGTCAGGGGG
 2641 GCGGAGCCTA TGGAAAAACG CCAGCAACGC GGCCTTTTTA CGGTTCCTGG CCTTTTGCTG
 2701 GCCTTTTGCT CACATGTT
//

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