| 质粒名称: | pMXs-hKLF4 |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 17219 |
| 存储实验室: | Shinya Yamanaka |
|
质粒骨架基本信息 |
|
| 质粒名称: | pMXs |
| 质粒抗性: | 氨苄霉素 |
| 空质粒大小: | 4600 bp |
| 质粒类型: | 哺乳细胞表达 |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | KLF4 |
| 物种来源: | H. sapiens (human) |
| 插入大小: | 1420 bp |
| 基因突变: | None |
| 克隆方法: | 限制性内切酶 |
| 5' 克隆位点: | attB1(未破坏) |
| 3' 克隆位点: | attB2(未破坏) |
| 5' 测序引物 | pMX-S1811 |
| 3' 测序引物 | |
|
文献引用方法 |
|
| 材料和方法部分: | pMXs-hKLF4 was a gift from Shinya Yamanaka (Addgene plasmid # 17219) |
| 参考文献部分: | Induction of pluripotent stem cells from adult human fibroblasts by defined factors. Takahashi K, Tanabe K, Ohnuki M, Narita M, Ichisaka T, Tomoda K, Yamanaka S. Cell. 2007 Nov 30. 131(5):861-72. 10.1016/j.cell.2007.11.019 PubMed 18035408 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pMXs-hKLF4 Sequencing Result 6180 bp DNA SYN
DEFINITION pMXs-hKLF4 Sequencing Result
ACCESSION
KEYWORDS
SOURCE
ORGANISM other sequences; artificial sequences; vectors.
FEATURES Location/Qualifiers
source 1..6180
/organism="pMXs-hKLF4 Sequencing Result"
/mol_type="other DNA"
misc_feature complement(130..711)
/label="3MoMuLV_LTR"
misc_feature complement(130..669)
/label="5_LTR2"
misc_feature complement(906..930)
/label="attB2"
CDS complement(947..2359)
/label="ORF frame 3"
CDS 1151..2002
/label="ORF frame 2"
misc_feature complement(2380..2404)
/label="attB1"
misc_feature complement(2453..3734)
/label="psi_plus_pack2"
misc_feature complement(2501..2517)
/label="pBMN_5_primer"
misc_feature complement(2770..3249)
/label="gag"
misc_feature complement(2855..2871)
/label="pBABE_5_primer"
misc_feature complement(2887..2909)
/label="MSCV_primer"
misc_feature complement(2887..2909)
/label="pLXSN_5_primer"
misc_feature complement(3734..4252)
/label="3MoMuLV_LTR"
misc_feature complement(3734..4210)
/label="5_LTR2"
promoter 4317..4345
/label="AmpR_promoter"
gene 4387..5247
/label="Ampicillin"
/gene="Ampicillin"
CDS 4387..5247
/label="ORF frame 1"
rep_origin 5395..6014
/label="pBR322_origin"
ORIGIN
1 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgCAACTATG
61 GCCATTTAAT GTAAATACTT AAGAAAAAAA ACCAAATTAA TTTTGATACA TGCTGCATGT
121 GAAAGACCCC CGCTGACGGG TAGTCAATCA CTCAGAGGAG ACCCTCCCAA GGAACAGCGA
181 GACCACAAGT CGGATGCAAC TGCAAGAGGG TTTATTGGAT ACACGGGTAC CCGGGCGACT
241 CAGTCAATCG GAGGACTGGC GCGCCGAGTG AGGGGTTGTG GGCTCTTTTA TTGAGCTCGG
301 GGAGCAGAAG CGCGCGAACA GAAGCGAGAA GCGAACTGAT TGGTTAGTTC AAATAAGGCA
361 CAGGGTCATT TCAGGTCCTT GGGGCACCCT GGAAACATCT GATGGTTCTC TAGAAACTGC
421 TGAGGGCTGG ACCGCATCTG GGGACCATCT GTTCTTGGCC CTGAGCCGGG GCAGGAACTG
481 CTTACCACAG ATATCCTGTT TGGCCCATAT TCAGCTGTTC CATCTGTTCT TGGCCCTGAG
541 CCGGGGCAGG AACTGCTTAC CACAGATATC CTGTTTGGCC CATATTCAGC TGTTCCATCT
601 GTTCCTGACC TTGATCTGAA CTTCTCTATT CTCAGTTATG TATTTTTCCA TGCCTTGCAA
661 AATGGCGTTA CTTAAGCTAG CTTGCCAAAC CTACAGGTGG GGTCTTTCAT TCCCCCCTTT
721 TTCTGGAGAC TAAATAAAAT CTTTTATTTT ATCGTCGACC ACTGTGCTGG CGGCCGCTCG
781 AGTTTAAATA GTACATAATG GATTTCCTTA CGCGAAATAC GGGCAGACAT GGCCTGCCCG
841 GTTATTATTA TTTTTGACAC CAGACCAACT GGTAATGGTA GCGACCGGCG CTCAGCTGGA
901 ATATCACCAC TTTGTACAAG AAAGCTGGGT CGGCGCGCCC ACCCTTTTAA AAATGTCTCT
961 TCATGTGTAA GGCGAGGTGG TCCGACCTGG AAAATGCTCG GTCGCATTTT TGGCACTGGA
1021 ACGGGCGGTG CCCCGTGTGT TTACGGTAGT GCCTGGTCAG TTCATCTGAG CGGGCGAATT
1081 TCCATCCACA GCCGTCCCAG TCACAGTGGT AAGGTTTCTC ACCTGTGTGG GTTCGCAGGT
1141 GTGCCTTGAG ATGGGAACTC TTTGTGTAGG TTTTGCCGCA GCCCGCGTAA TCACAAGTGT
1201 GGGTGGCGGT CCTTTTCCGG GGCCACGATC GTCTTCCCCT CTTTGGCTTG GGCTCCTCTG
1261 GCATGCAGGA ACCGGGTGGC ATGAGCTCTT GGTAATGGAG CGGCGGGACT TGCGGCTGCA
1321 TCTGATCGGG CAGGAAGGAT GGGTAATTGG GCCCCGGGTG GGGATGGAAG CCGGGAGGAA
1381 GCGGCAGGGC AGGGTGACAG TCCCTGCTGC TCAGCACTTC CTCAAGACCC AGGGTCGGGG
1441 TAGTCCTGCT GGGGAGCTGC CGCCCCAGGG GGAAGTCGTG TGCAGCCGGC CGGTGGCCAT
1501 TGCTGAGAGG GGGTCCAGCG CCCAAGTGGG TGCACGAAGA GACCGCCTCC TGCTTGATCT
1561 TGGGGCACGT GCGCGGCGGC CCGCCGTTGT AGGGCGCCAC CACCACCGGG TGGCTGCCGT
1621 CAGGGCTGCC TTTGCTGACG CTGATGACCG ACGGGCTGCC GTACTCGCTG CCAGGGGCGC
1681 TCAGCGACGC CTTCAGCACG AACTTGCCCA TCAGCCCGCC ACCTGGCGGC TGCGGCTGCT
1741 GCGGCGGAAT GTACACCGGG TCCAATTCTG GCCGCAGGAG CTCGGCCACG AAGCCGCCCG
1801 AGGGGCTCAC GTCGTTGATG TCCGCCAGGT TGAAGGGAGC CGTCGGAGGG GGAGCGGACT
1861 CCCTGCCATA GAGGAGGCCT CCGCCCGTGC CGCCCGGCGC CACGCCCGGG TCGTTCCCGG
1921 CCCGGATCGG ATAGGTGAAG CTGCAGGTGG AGGGCGCGCT GGCAGGGCCG CTGCTCGACG
1981 GCGACGACGA AGAGGAGGCT GACGCTGACG AGGACACGGT GGCGGCCACT GACTCCGGAG
2041 GATGGGTCAG CGAATTGGAG AGAATAAAGT CCAGGTCCAG GAGATCGTTG AACTCCTCGG
2101 TCTCTCTCCG AGGTAGGGGC GCCAGGTTGC TACCGCCGCA AGCCGCACCG GCTCCGCCGC
2161 TCTCCAGGTC TGTGGCCACG GTCGCCGCCG CCAGGTCATA GGGGCGGCCG GGAAGCACTG
2221 GGGGAAGTCG CTTCATGTGG GAGAGCTCCT CCCGCCAGCG GTTATTCGGG GCACCTGCTT
2281 GACGCAGTGT CTTCTCCCTT CCCGCCGGGC CAGACGCGAA CGTGGAGAAA GATGGGAGCA
2341 GCGCGTCACT GACAGCCATG GTGAAGGGGG CGGCCGCGGA GCCTGCTTTT TTGTACAAAC
2401 TTGTGATTTC CCGTACCACC ACACTGGGAT CCTTAATTAA CTAGCTAGAT CCGGCAGTCT
2461 AGAGGATGGT CCACCCCCGG GGTCGGCAGC CTTCACGTGG GCGGCGTGTA TCCAAGCTGC
2521 GATGCCGTCT ACTTTGAGGG CGGTGGGGGT GGTCAGCAGG ACTGTGTAAG GTCCTTTCCA
2581 GCGAGGTTCT AGGTTCTTAG TCTGGTGTCG GCGGACCCAC ACTGTGTCGC CGACTCGGTA
2641 AGGGTGAGGT ACCACCGGTC GGTCCAGTTG TTCTTGGTAG GCTGCCGCCA GAGGTCTCCA
2701 GACTTCGTGC TGGACTAAGT AGAGAGCCTG TAAGTGAGCT TGGAGAGAGG GGCTGTTAGT
2761 AACTCTTGTC ATGTCAGGGT CAGGGAAGTT TACAAGGGGC GGGGGTGCCC CATATAAGAT
2821 CTCATATGGC CATATGGGGG CGCCTAGAGA AGGAGTGAGG GCTGGATAAA GGGAGGATCG
2881 AGGCGGGGTC GAACGAGGAG GTTCAAGGGG GAGAGACGGG GCGGATGGAG GAAGAGGAGG
2941 CGGAGGCTTA GGGTGTACAA AGGGCTTGAC CCAGGGAGGG GGGTCAAAAG CCAAGGCTTC
3001 CCAGGTCACG ATGTAGGGGA CCTGGTCTGG GTGTCCATGC GGGCCAGGTG AAAAGACCTT
3061 GATCTTAACC TGGGTGATGA GGTCTCGGTT AAAGGTGCCG TCTCGCGGCC ATCCGACGTT
3121 AAAGGTTGGC CATTCTGCAG AGCAGAAGGT AACCCAACGT CTCTTCTTGA CATCTACCGA
3181 CTGGTTGTGA GCGATCCGCT CGACATCTTT CCAGTGACCT AAGGTCAAAC TTAAGGGAGT
3241 GGTAACAGTC TGGCCCTAAT TTTCAGACAA ATACAGAAAC ACAGTCAGAC AGAGACAACA
3301 CAGAACGATG CTGCAGCAGA CAAGACGCGC GGCGCGGCTT CGGTCCCAAA CCGAAAGCAA
3361 AAATTCAGAC GGAGGCGGGA ACTGTTTTAG GTTCTCGTCT CCTACCAGAA CCACATATCC
3421 CTCCTATTAG GGGGGTGCAC CAAAGAGTCC AAAACGATCG GGATTTTTGG ACTCAGGTCG
3481 GGCCACAAAA ACGGCCCCCG AAGTCCCTGG GACGTCTCCC AGGGTTGCGG CCGGGTGTTC
3541 CGAACTCGTC AGTTCCACCA CGGGTCCGCC AGATACAGAG CTAGTTAGCT AACTAGTACC
3601 GACGCAGGCG CATAAAATCA GTCATAGACA CTAGACAATC GGACAGACAC AGATAAGTTG
3661 CTGGCCAGCT TACCTCCCGG TGGTGGGTCG GTGGTCCCTG GGCAGGGGTC TCCCGATCCC
3721 GGACGAGCCC CCAAATGAAA GACCCCCGCT GACGGGTAGT CAATCACTCA GAGGAGACCC
3781 TCCCAAGGAA CAGCGAGACC ACAAGTCGGA TGCAACTGCA AGAGGGTTTA TTGGATACAC
3841 GGGTACCCGG GCGACTCAGT CAATCGGAGG ACTGGCGCGC CGAGTGAGGG GTTGTGGGCT
3901 CTTTTATTGA GCTCGGGGAG CAGAAGCGCG CGAACAGAAG CGAGAAGCGA ACTGATTGGT
3961 TAGTTCAAAT AAGGCACAGG GTCATTTCAG GTCCTTGGGG CACCCTGGAA ACATCTGATG
4021 GTTCTCTAGA AACTGCTGAG GGCTGGACCG CATCTGGGGA CCATCTGTTC TTGGCCCTGA
4081 GCCGGGGCAG GAACTGCTTA CCACAGATAT CCTGTTTGGC CCATATTCAG CTGTTCCATC
4141 TGTTCCTGAC CTTGATCTGA ACTTCTCTAT TCTCAGTTAT GTATTTTTCC ATGCCTTGCA
4201 AAATGGCGTT ACTTAAGCTA GCTTGCCAAA CCTACAGGTG GGGTCTTTCA TTATGCAGGT
4261 GGCACTTTTC GGGGAAATGT GCGCGGAACC CCTATTTGTT TATTTTTCTA AATACATTCA
4321 AATATGTATC CGCTCATGAG ACAATAACCC TGATAAATGC TTCAATAATA TTGAAAAAGG
4381 AAGAGTATGA GTATTCAACA TTTCCGTGTC GCCCTTATTC CCTTTTTTGC GGCATTTTGC
4441 CTTCCTGTTT TTGCTCACCC AGAAACGCTG GTGAAAGTAA AAGATGCTGA AGATCAGTTG
4501 GGTGCACGAG TGGGTTACAT CGAACTGGAT CTCAACAGCG GTAAGATCCT TGAGAGTTTT
4561 CGCCCCGAAG AACGTTTTCC AATGATGAGC ACTTTTAAAG TTCTGCTATG TGGCGCGGTA
4621 TTATCCCGTA TTGACGCCGG GCAAGAGCAA CTCGGTCGCC GCATACACTA TTCTCAGAAT
4681 GACTTGGTTG AGTACTCACC AGTCACAGAA AAGCATCTTA CGGATGGCAT GACAGTAAGA
4741 GAATTATGCA GTGCTGCCAT AACCATGAGT GATAACACTG CGGCCAACTT ACTTCTGACA
4801 ACGATCGGAG GACCGAAGGA GCTAACCGCT TTTTTGCACA ACATGGGGGA TCATGTAACT
4861 CGCCTTGATC GTTGGGAACC GGAGCTGAAT GAAGCCATAC CAAACGACGA GCGTGACACC
4921 ACGATGCCTG TAGCAATGGC AACAACGTTG CGCAAACTAT TAACTGGCGA ACTACTTACT
4981 CTAGCTTCCC GGCAACAATT AATAGACTGG ATGGAGGCGG ATAAAGTTGC AGGACCACTT
5041 CTGCGCTCGG CCCTTCCGGC TGGCTGGTTT ATTGCTGATA AATCTGGAGC CGGTGAGCGT
5101 GGGTCTCGCG GTATCATTGC AGCACTGGGG CCAGATGGTA AGCCCTCCCG TATCGTAGTT
5161 ATCTACACGA CGGGGAGTCA GGCAACTATG GATGAACGAA ATAGACAGAT CGCTGAGATA
5221 GGTGCCTCAC TGATTAAGCA TTGGTAACTG TCAGACCAAG TTTACTCATA TATACTTTAG
5281 ATTGATTTGC GGCCGGCCGC AAAAGGATCT AGGTGAAGAT CCTTTTTGAT AATCTCATGA
5341 CCAAAATCCC TTAACGTGAG TTTTCGTTCC ACTGAGCGTC AGACCCCGTA GAAAAGATCA
5401 AAGGATCTTC TTGAGATCCT TTTTTTCTGC GCGTAATCTG CTGCTTGCAA ACAAAAAAAC
5461 CACCGCTACC AGCGGTGGTT TGTTTGCCGG ATCAAGAGCT ACCAACTCTT TTTCCGAAGG
5521 TAACTGGCTT CAGCAGAGCG CAGATACCAA ATACTGTTCT TCTAGTGTAG CCGTAGTTAG
5581 GCCACCACTT CAAGAACTCT GTAGCACCGC CTACATACCT CGCTCTGCTA ATCCTGTTAC
5641 CAGTGGCTGC TGCCAGTGGC GATAAGTCGT GTCTTACCGG GTTGGACTCA AGACGATAGT
5701 TACCGGATAA GGCGCAGCGG TCGGGCTGAA CGGGGGGTTC GTGCACACAG CCCAGCTTGG
5761 AGCGAACGAC CTACACCGAA CTGAGATACC TACAGCGTGA GCTATGAGAA AGCGCCACGC
5821 TTCCCGAAGG GAGAAAGGCG GACAGGTATC CGGTAAGCGG CAGGGTCGGA ACAGGAGAGC
5881 GCACGAGGGA GCTTCCAGGG GGAAACGCCT GGTATCTTTA TAGTCCTGTC GGGTTTCGCC
5941 ACCTCTGACT TGAGCGTCGA TTTTTGTGAT GCTCGTCAGG GGGGCGGAGC CTATGGAAAA
6001 ACGCCAGCAA CGCGGCCTTT TTACGGTTCC TGGCCTTTTG CTGGCCTTTT GCTCACATGT
6061 TCTTTCCTGC GTTATCCCCT GATTCTGTGG ATAACCGTAT TACCGCCTTT GAGTGAGCTG
6121 ATACCGCTCG CCGCAGCCGA ACGACCGAGC GCAGCGAGTC AGTGAGCGAG GAAGCGGAAG
//
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