| 质粒名称: | pMXs-hc-MYC |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 17220 |
| 存储实验室: | Shinya Yamanaka |
|
质粒骨架基本信息 |
|
| 质粒名称: | pMXs |
| 质粒抗性: | 氨苄霉素 |
| 空质粒大小: | 4600 bp |
| 质粒类型: | 哺乳细胞表达 |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | c-MYC |
| 物种来源: | H. sapiens (human) |
| 插入大小: | 1320 bp |
| 基因突变: | None |
| GenBank编号 | BAA01375.1 |
| 克隆方法: | 限制性内切酶 |
| 5' 克隆位点: | attB1(未破坏) |
| 3' 克隆位点: | attB2(未破坏) |
| 5' 测序引物 | pMX-S1811 |
|
文献引用方法 |
|
| 材料和方法部分: | pMXs-hc-MYC was a gift from Shinya Yamanaka (Addgene plasmid # 17220) |
| 参考文献部分: | Induction of pluripotent stem cells from adult human fibroblasts by defined factors. Takahashi K, Tanabe K, Ohnuki M, Narita M, Ichisaka T, Tomoda K, Yamanaka S. Cell. 2007 Nov 30. 131(5):861-72. 10.1016/j.cell.2007.11.019 PubMed 18035408 |
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载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pMXs-hc-MYC Sequencing Result 6162 bp DNA SYN
DEFINITION pMXs-hc-MYC Sequencing Result
ACCESSION
KEYWORDS
SOURCE
ORGANISM other sequences; artificial sequences; vectors.
FEATURES Location/Qualifiers
source 1..6162
/organism="pMXs-hc-MYC Sequencing Result"
/mol_type="other DNA"
misc_feature complement(130..711)
/label="3MoMuLV_LTR"
misc_feature complement(130..669)
/label="5_LTR2"
misc_feature complement(906..930)
/label="attB2"
CDS complement(947..2266)
/label="ORF frame 3"
misc_feature complement(1010..1039)
/label="Myc"
misc_feature complement(2287..2311)
/label="attB1"
misc_feature complement(2360..3641)
/label="psi_plus_pack2"
misc_feature complement(2408..2424)
/label="pBMN_5_primer"
misc_feature complement(2677..3156)
/label="gag"
misc_feature complement(2762..2778)
/label="pBABE_5_primer"
misc_feature complement(2794..2816)
/label="MSCV_primer"
misc_feature complement(2794..2816)
/label="pLXSN_5_primer"
misc_feature complement(3641..4234)
/label="3MoMuLV_LTR"
misc_feature complement(3641..4192)
/label="5_LTR2"
promoter 4299..4327
/label="AmpR_promoter"
gene 4369..5229
/label="Ampicillin"
/gene="Ampicillin"
CDS 4369..5229
/label="ORF frame 1"
rep_origin 5377..5996
/label="pBR322_origin"
ORIGIN
1 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgCAACTATG
61 GCCATTTAAT GTAAATACTT AAGAAAAAAA ACCAAATTAA TTTTGATACA TGCTGCATGT
121 GAAAGACCCC CGCTGACGGG TAGTCAATCA CTCAGAGGAG ACCCTCCCAA GGAACAGCGA
181 GACCACAAGT CGGATGCAAC TGCAAGAGGG TTTATTGGAT ACACGGGTAC CCGGGCGACT
241 CAGTCAATCG GAGGACTGGC GCCCCGAGTG AGGGGTTGTG GGCTCTTTTA TTGAGCTCGG
301 GGAGCAGAAG CGCGCGAACA GAAGCGAGAA GCGAACTGAT TGGTTAGTTC AAATAAGGCA
361 CAGGGTCATT TCAGGTCCTT GGGGCACCCT GGAAACATCT GATGGTTCTC TAGAAACTGC
421 TGAGGGCTGG ACCGCATCTG GGGACCATCT GTTCTTGGCC CTGAGCCGGG GCAGGAACTG
481 CTTACCACAG ATATCCTGTT TGGCCCATAT TCAGCTGTTC CATCTGTTCT TGGCCCTGAG
541 CCGGGGCAGG AACTGCTTAC CACAGATATC CTGTTTGGCC CATATTCAGC TGTTCCATCT
601 GTTCCTGACC TTGATCTGAA CTTTTCTATT CTCAGTTATG TATTTTTCCA TGCCTTGCAA
661 AATGGCGTTA CTTAAGCTAG CTTGCCAAAC CTACAGGTGG GGTCTTTCAT TCCCCCCTTT
721 TTCTGGAGAC TAAATAAAAT CTTTTATTTT ATCGTCGACC ACTGTGCTGG CGGCCGCTCG
781 AGTTTAAATA GTACATAATG GATTTCCTTA CGCGAAATAC GGGCAGACAT GGCCTGCCCG
841 GTTATTATTA TTTTTGACAC CAGACCAACT GGTAATGGTA GCGACCGGCG CTCAGCTGGA
901 ATATCACCAC TTTGTACAAG AAAGCTGGGT CGGCGCGCCC ACCCTTTCAC GCACAAGAGT
961 TCCGTAGCTG TTCAAGTTTG TGTTTCAACT GTTCTCGTCG TTTCCGCAAC AAGTCCTCTT
1021 CAGAAATGAG CTTTTGCTCC TCTGCTTGGA CGGACAGGAT GTATGCTGTG GCTTTTTTAA
1081 GGATAACTAC CTTGGGGGCC TTTTCATTGT TTTCCAACTC CGGGATCTGG TCACGCAGGG
1141 CAAAAAAGCT CCGTTTTAGC TCGTTCCTCC TCTGGCGCTC CAAGACGTTG TGTGTTCGCC
1201 TCTTGACATT CTCCTCGGTG TCCGAGGACC TGGGGCTGGT GCATTTTCGG TTGTTGCTGA
1261 TCTGTCTCAG GACTCTGACA CTGTCCAACT TGACCCTCTT GGCAGCAGGA TAGTCCTTCC
1321 GAGTGGAGGG AGGCGCTGCG TAGTTGTGCT GATGTGTGGA GACGTGGCAC CTCTTGAGGA
1381 CCAGTGGGCT GTGAGGAGGT TTGCTGTGGC CTCCAGCAGA AGGTGATCCA GACTCTGACC
1441 TTTTGCCAGG AGCCTGCCTC TTTTCCACAG AAACAACATC GATTTCTTCC TCATCTTCTT
1501 GTTCCTCCTC AGAGTCGCTG CTGGTGGTGG GCGGTGTCTC CTCATGGAGC ACCAGGGGCT
1561 CGGGGCTGCC CTGCGGGGAG GACTCCGTCG AGGAGAGCAG AGAATCCGAG GACGGAGAGA
1621 AGGCGCTGGA GTCTTGCGAG GCGCAGGACT TGGGCGAGCT GCTGTCGTTG AGAGGGTAGG
1681 GGAAGACCAC CGAGGGGTCG ATGCACTCTG AGGCGGCGGC GCTCAGATCC TGCAGGTACA
1741 AGCTGGAGGT GGAGCAGACG CTGTGGCCGC GGGCGGGGTT CGGGCTGCCG CTGTCTTTGC
1801 GCGCAGCCTG GTAGGAGGCC AGCTTCTCTG AGACGAGCTT GGCGGCGGCC GAGAAGCCGC
1861 TCCACATACA GTCCTGGATG ATGATGTTTT TGATGAAGGT CTCGTCGTCC GGGTCGCAGA
1921 TGAAACTCTG GTTCACCATG TCTCCTCCCA GCAGCTCGGT CACCATCTCC AGCTGGTCGG
1981 CCGTGGAGAA GCTCCCGCCA CCGCCGTCGT TGTCTCCCCG AAGGGAGAAG GGTGTGACCG
2041 CAACGTAGGA GGGCGAGCAG AGCCCGGAGC GGCGGCTAGG GGACAGGGGC GGGGTGGGCA
2101 GCAGCTCGAA TTTCTTCCAG ATATCCTCGC TGGGCGCCGG GGGCTGCAGC TCGCTCTGCT
2161 GCTGCTGCTG GTAGAAGTTC TCCTCCTCGT CGCAGTAGAA ATACGGCTGC ACCGAGTCGT
2221 AGTCGAGGTC ATAGTTCCTG TTGGTGAAGC TAACGTTGAG GGGCATGGTG AAGGGGGCGG
2281 CCGCGGAGCC TGCTTTTTTG TACAAACTTG TGATTTCCCG TACCACCACA CTGGGATCCT
2341 TAATTAACTA GCTAGATCCG GCAGTCTAGA GGATGGTCCA CCCCCGGGGT CGGCAGCCTT
2401 CACGTGGGCG GCGTGTATCC AAGCTGCGAT GCCGTCTACT TTGAGGGCGG TGGGGGTGGT
2461 CAGCAGGACT GTGTAAGGTC CTTTCCAGCG AGGTTCTAGG TTCTTAGTCT GGTGTCGGCG
2521 GACCCACACT GTGTCGCCGA CTCGGTAAGG GTGAGGTACC ACCGGTCGGT CCAGTTGTTC
2581 TTGGTAGGCT GCCGCCAGAG GTCTCCAGAC TTCGTGCTGG ACTAAGTAGA GAGCCTGTAA
2641 GTGAGCTTGG AGAGAGGGGC TGTTAGTAAC TCTTGTCATG TCAGGGTCAG GGAAGTTTAC
2701 AAGGGGCGGG GGTGCCCCAT ATAAGATCTC ATATGGCCAT ATGGGGGCGC CTAGAGAAGG
2761 AGTGAGGGCT GGATAAAGGG AGGATCGAGG CGGGGTCGAA CGAGGAGGTT CAAGGGGGAG
2821 AGACGGGGCG GATGGAGGAA GAGGAGGCGG AGGCTTAGGG TGTACAAAGG GCTTGACCCA
2881 GGGAGGGGGG TCAAAAGCCA AGGCTTCCCA GGTCACGATG TAGGGGACCT GGTCTGGGTG
2941 TCCATGCGGG CCAGGTGAAA AGACCTTGAT CTTAACCTGG GTGATGAGGT CTCGGTTAAA
3001 GGTGCCGTCT CGCGGCCATC CGACGTTAAA GGTTGGCCAT TCTGCAGAGC AGAAGGTAAC
3061 CCAACGTCTC TTCTTGACAT CTACCGACTG GTTGTGAGCG ATCCGCTCGA CATCTTTCCA
3121 GTGACCTAAG GTCAAACTTA AGGGAGTGGT AACAGTCTGG CCCTAATTTT CAGACAAATA
3181 CAGAAACACA GTCAGACAGA GACAACACAG AACGATGCTG CAGCAGACAA GACGCGCGGC
3241 GCGGCTTCGG TCCCAAACCG AAAGCAAAAA TTCAGACGGA GGCGGGAACT GTTTTAGGTT
3301 CTCGTCTCCT ACCAGAACCA CATATCCCTC CTATTAGGGG GGTGCACCAA AGAGTCCAAA
3361 ACGATCGGGA TTTTTGGACT CAGGTCGGGC CACAAAAACG GCCCCCGAAG TCCCTGGGAC
3421 GTCTCCCAGG GTTGCGGCCG GGTGTTCCGA ACTCGTCAGT TCCACCACGG GTCCGCCAGA
3481 TACAGAGCTA GTTAGCTAAC TAGTACCGAC GCAGGCGCAT AAAATCAGTC ATAGACACTA
3541 GACAATCGGA CAGACACAGA TAAGTTGCTG GCCAGCTTAC CTCCCGGTGG TGGGTCGGTG
3601 GTCCCTGGGC AGGGGTCTCC CGATCCCGGA CGAGCCCCCA AATGAAAGAC CCCCGCTGAC
3661 GGGTAGTCAA TCACTCAGAG GAGACCCTCC CAAGGAACAG CGAGACCACA AGTCGGATGC
3721 AACTGCAAGA GGGTTTATTG GATACACGGG TACCCGGGCG ACTCAGTCAA TCGGAGGACT
3781 GGCGCCCCGA GTGAGGGGTT GTGGGCTCTT TTATTGAGCT CGGGGAGCAG AAGCGCGCGA
3841 ACAGAAGCGA GAAGCGAACT GATTGGTTAG TTCAAATAAG GCACAGGGTC ATTTCAGGTC
3901 CTTGGGGCAC CCTGGAAACA TCTGATGGTT CTCTAGAAAC TGCTGAGGGC TGGACCGCAT
3961 CTGGGGACCA TCTGTTCTTG GCCCTGAGCC GGGGCAGGAA CTGCTTACCA CAGATATCCT
4021 GTTTGGCCCA TATTCAGCTG TTCCATCTGT TCTTGGCCCT GAGCCGGGGC AGGAACTGCT
4081 TACCACAGAT ATCCTGTTTG GCCCATATTC AGCTGTTCCA TCTGTTCCTG ACCTTGATCT
4141 GAACTTTTCT ATTCTCAGTT ATGTATTTTT CCATGCCTTG CAAAATGGCG TTACTTAAGC
4201 TAGCTTGCCA AACCTACAGG TGGGGTCTTT CATTATGCAG GTGGCACTTT TCGGGGAAAT
4261 GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG
4321 AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA
4381 CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC
4441 CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC
4501 ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT
4561 CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TATTGACGCC
4621 GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA
4681 CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC
4741 ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG
4801 GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA
4861 CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGTAGCAATG
4921 GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA
4981 TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG
5041 GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT
5101 GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT
5161 CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG
5221 CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT GCGGCCGGCC
5281 GCAAAAGGAT CTAGGTGAAG ATCCTTTTTG ATAATCTCAT GACCAAAATC CCTTAACGTG
5341 AGTTTTCGTT CCACTGAGCG TCAGACCCCG TAGAAAAGAT CAAAGGATCT TCTTGAGATC
5401 CTTTTTTTCT GCGCGTAATC TGCTGCTTGC AAACAAAAAA ACCACCGCTA CCAGCGGTGG
5461 TTTGTTTGCC GGATCAAGAG CTACCAACTC TTTTTCCGAA GGTAACTGGC TTCAGCAGAG
5521 CGCAGATACC AAATACTGTT CTTCTAGTGT AGCCGTAGTT AGGCCACCAC TTCAAGAACT
5581 CTGTAGCACC GCCTACATAC CTCGCTCTGC TAATCCTGTT ACCAGTGGCT GCTGCCAGTG
5641 GCGATAAGTC GTGTCTTACC GGGTTGGACT CAAGACGATA GTTACCGGAT AAGGCGCAGC
5701 GGTCGGGCTG AACGGGGGGT TCGTGCACAC AGCCCAGCTT GGAGCGAACG ACCTACACCG
5761 AACTGAGATA CCTACAGCGT GAGCTATGAG AAAGCGCCAC GCTTCCCGAA GGGAGAAAGG
5821 CGGACAGGTA TCCGGTAAGC GGCAGGGTCG GAACAGGAGA GCGCACGAGG GAGCTTCCAG
5881 GGGGAAACGC CTGGTATCTT TATAGTCCTG TCGGGTTTCG CCACCTCTGA CTTGAGCGTC
5941 GATTTTTGTG ATGCTCGTCA GGGGGGCGGA GCCTATGGAA AAACGCCAGC AACGCGGCCT
6001 TTTTACGGTT CCTGGCCTTT TGCTGGCCTT TTGCTCACAT GTTCTTTCCT GCGTTATCCC
6061 CTGATTCTGT GGATAACCGT ATTACCGCCT TTGAGTGAGC TGATACCGCT CGCCGCAGCC
6121 GAACGACCGA GCGCAGCGAG TCAGTGAGCG AGGAAGCGGA AG
//
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