| 质粒名称: | pCasKP-apr |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 117231 |
| 存储实验室: | Quanjiang Ji |
|
载体骨架基本信息 |
|
| 载体名称: | -- |
| 载体出品公司: | -- |
| 载体抗性: | 安普霉素 |
| 空载体大小: | -- |
| 完整质粒大小: | 11409 bp |
| 载体修饰: | -- |
| 载体类型: | 细菌表达，CRISPR，合成生物学 |
| 筛选标记: | -- |
| 高拷贝/低拷贝: | 低拷贝 |
| 生长条件: | 30 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | Cas9 |
| 物种来源: | -- |
| 插入大小: | -- |
| 基因突变: | -- |
| GenBank编号 | -- |
| 启动子 | -- |
| 标签/融合蛋白: | -- |
| 克隆方法: | Gibson克隆 |
| 5' 克隆位点: | -- |
| 3' 克隆位点: | -- |
| 5' 测序引物 | -- |
| 3' 测序引物 | -- |
|
文献引用方法 |
|
| 材料和方法部分: | pCasKP-apr was a gift from Quanjiang Ji (Addgene plasmid # 117231 ; http://n2t.net/addgene:117231 ; RRID:Addgene_117231) |
| 参考文献部分: | Precise and efficient genome editing in Klebsiella pneumoniae using CRISPR-Cas9 and CRISPR-assisted cytidine deaminase. Wang Y, Wang S, Chen W, Song L, Zhang Y, Shen Z, Yu F, Li M, Ji Q. Appl Environ Microbiol. 2018 Sep 14. pii: AEM.01834-18. doi: 10.1128/AEM.01834-18. 10.1128/AEM.01834-18 PubMed 30217854 |
买家导航
pCasKP-apr载体 pCasKPapr质粒基本信息
pCasKP-apr质粒图谱 pCasKPapr载体图谱和pCasKP-apr载体序列 pCasKPapr质粒序列多克隆位点信息
pCasKP-apr质粒 pCasKPapr载体简介
pCasKP-apr质粒序列 pCasKPapr载体序列
LOCUS pCasKP-apr 11393 bp ds-DNA circular SYN 12-MAY-2021
DEFINITION Bacterial expression of Cas9 nuclease and lambda-Red system in
Klebsiella Pneumoniae.
ACCESSION .
VERSION .
KEYWORDS pCasKP-apr
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 11393)
AUTHORS Wang Y, Wang S, Chen W, Song L, Zhang Y, Shen Z, Yu F, Li M, Ji Q
TITLE Precise and efficient genome editing in Klebsiella pneumoniae using
CRISPR-Cas9 and CRISPR-assisted cytidine deaminase.
JOURNAL Appl Environ Microbiol. 2018 Sep 14. pii: AEM.01834-18. doi:
10.1128/AEM.01834-18.
PUBMED 30217854
REFERENCE 2 (bases 1 to 11393)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..11393
/organism="synthetic DNA construct"
/mol_type="other DNA"
promoter 1..285
/gene="araBAD"
/label=araBAD promoter
/note="promoter of the L-arabinose operon of E. coli; the
araC regulatory gene is transcribed in the opposite
direction (Guzman et al., 1995)"
primer_bind 208..227
/label=pBAD Forward
/note="For vectors with E. coli araBAD promoter, forward
primer"
CDS 344..760
/codon_start=1
/gene="gam"
/product="inhibitor of the host RecBCD nuclease in the
lambda Red system"
/label=Gam
/note="protects the double-stranded DNA substrate for
recombination"
/translation="MDINTETEIKQKHSLTPFPVFLISPAFRGRYFHSYFRSSAMNAYY
IQDRLEAQSWARHYQQLAREEKEAELADDMEKGLPQHLFESLCIDHLQRHGASKKSITR
AFDDDVEFQERMAEHIRYMVETIAHHQVDIDSEV"
CDS 766..1551
/codon_start=1
/gene="bet"
/product="single-stranded DNA binding recombinase in the
lambda Red system"
/label=Beta
/note="mediates pairing of a single-stranded DNA with a
complementary target"
/translation="MSTALATLAGKLAERVGMDSVDPQELITTLRQTAFKGDASDAQFI
ALLIVANQYGLNPWTKEIYAFPDKQNGIVPVVGVDGWSRIINENQQFDGMDFEQDNESC
TCRIYRKDRNHPICVTEWMDECRREPFKTREGREITGPWQSHPKRMLRHKAMIQCARLA
FGFAGIYDKDEAERIVENTAYTAERQPERDITPVNDETMQEINTLLIALDKTWDDDLLP
LCSQIFRRDIRASSELTQAEAVKALGFLKQKAAEQKVAA"
CDS 1548..2228
/codon_start=1
/gene="exo"
/product="5' to 3' double-stranded DNA exonuclease in the
lambda Red system"
/label=Exo
/note="creates 3' single-stranded DNA tails"
/translation="MTPDIILQRTGIDVRAVEQGEDAWHKLRLGVITASEVHNVIAKPR
SGKKWPDMKMSYFHTLLAEVCTGVAPEVNAKALAWGKQYENDARTLFEFTSGVNVTESP
IIYRDESMRTACSPDGLCSDGNGLELKCPFTSRDFMKFRLGGFEAIKSAYMAQVQYSMW
VTRKNAWYFANYDPRMKREGLHYVVIERDEKYMASFDEIVPEFIEKMDEALAEIGFVFG
EQWR"
CDS 2810..6916
/codon_start=1
/product="Cas9 (Csn1) endonuclease from the Streptococcus
pyogenes Type II CRISPR/Cas system"
/label=Cas9
/note="generates RNA-guided double strand breaks in DNA"
/translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
DATLIHQSITGLYETRIDLSQLGGD"
CDS complement(7051..8001)
/codon_start=1
/gene="rep101 encoding an A56V mutation"
/product="temperature-sensitive version of the RepA protein
needed for replication with the pSC101 origin (Armstrong et
al., 1984)"
/label=Rep101(Ts)
/translation="MSELVVFKANELAISRYDLTEHETKLILCCVALLNPTIENPTRKE
RTVSFTYNQYVQMMNISRENAYGVLAKATRELMTRTVEIRNPLVKGFEIFQWTNYAKFS
SEKLELVFSEEILPYLFQLKKFIKYNLEHVKSFENKYSMRIYEWLLKELTQKKTHKANI
EISLDEFKFMLMLENNYHEFKRLNQWVLKPISKDLNTYSNMKLVVDKRGRPTDTLIFQV
ELDRQMDLVTELENNQIKMNGDKIPTTITSDSYLHNGLRKTLHDALTAKIQLTSFEAKF
LSDMQSKYDLNGSFSWLTQKQRTTLENILAKYGRI"
rep_origin 8049..8271
/label=pSC101 ori
/note="low-copy replication origin that requires the Rep101
protein"
promoter 8901..9003
/label=cat promoter
/note="promoter of the E. coli cat gene encoding
chloramphenicol acetyltransferase"
primer_bind complement(9070..9089)
/label=CAT-R
/note="Chloramphenicol resistance gene, reverse primer"
CDS 9459..10259
/codon_start=1
/gene="aac(3)IV"
/product="aminoglycoside 3-N-acetyltransferase type IV
"
/label=ApmR
/note="confers resistance to apramycin (Paget and Davies,
1996)"
/translation="MSSAVECNVVQYEWRKAELIGQLLNLGVTPGGVLLVHSSFRSVRP
LEDGPLGLIEALRAALGPGGTLVMPSWSGLDDEPFDPATSPVTPDLGVVSDTFWRLPNV
KRSAHPFAFAAAGPQAEQIISDPLPLPPHSPASPVARVHELDGQVLLLGVGHDANTTLH
LAELMAKVPYGVPRHCTILQDGKLVRVDYLENDHCCERFALADRWLKEKSLQKEGPVGH
AFARLIRSRDIVATALGQLGRDPLIFLHPPEAGCEECDAARQSIG"
CDS complement(10489..11367)
/codon_start=1
/gene="araC"
/product="L-arabinose regulatory protein"
/label=araC
/translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKGY
ILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRAYW
HEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLLLRR
MEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQLGIS
VLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEFRAGCE
EKVNDVAVKLS"
ORIGIN
1 aagaaaccaa ttgtccatat tgcatcagac attgccgtca ctgcgtcttt tactggctct
61 tctcgctaac caaaccggta accccgctta ttaaaagcat tctgtaacaa agcgggacca
121 aagccatgac aaaaacgcgt aacaaaagtg tctataatca cggcagaaaa gtccacattg
181 attatttgca cggcgtcaca ctttgctatg ccatagcatt tttatccata agattagcgg
241 atcctacctg acgcttttta tcgcaactct ctactgtttc tccatacccg tttttttggg
301 ctagcgaaaa gatgtttcgt gaagccgtcg acgcttataa aaaatggata ttaatactga
361 aactgagatc aagcaaaagc attcactaac cccctttcct gttttcctaa tcagcccggc
421 atttcgcggg cgatattttc acagctattt caggagttca gccatgaacg cttattacat
481 tcaggatcgt cttgaggctc agagctgggc gcgtcactac cagcagctcg cccgtgaaga
541 gaaagaggca gaactggcag acgacatgga aaaaggcctg ccccagcacc tgtttgaatc
601 gctatgcatc gatcatttgc aacgccacgg ggccagcaaa aaatccatta cccgtgcgtt
661 tgatgacgat gttgagtttc aggagcgcat ggcagaacac atccggtaca tggttgaaac
721 cattgctcac caccaggttg atattgattc agaggtataa aacgaatgag tactgcactc
781 gcaacgctgg ctgggaagct ggctgaacgt gtcggcatgg attctgtcga cccacaggaa
841 ctgatcacca ctcttcgcca gacggcattt aaaggtgatg ccagcgatgc gcagttcatc
901 gcattactga tcgttgccaa ccagtacggc cttaatccgt ggacgaaaga aatttacgcc
961 tttcctgata agcagaatgg catcgttccg gtggtgggcg ttgatggctg gtcccgcatc
1021 atcaatgaaa accagcagtt tgatggcatg gactttgagc aggacaatga atcctgtaca
1081 tgccggattt accgcaagga ccgtaatcat ccgatctgcg ttaccgaatg gatggatgaa
1141 tgccgccgcg aaccattcaa aactcgcgaa ggcagagaaa tcacggggcc gtggcagtcg
1201 catcccaaac ggatgttacg tcataaagcc atgattcagt gtgcccgtct ggccttcgga
1261 tttgctggta tctatgacaa ggatgaagcc gagcgcattg tcgaaaatac tgcatacact
1321 gcagaacgtc agccggaacg cgacatcact ccggttaacg atgaaaccat gcaggagatt
1381 aacactctgc tgatcgccct ggataaaaca tgggatgacg acttattgcc gctctgttcc
1441 cagatatttc gccgcgacat tcgtgcatcg tcagaactga cacaggccga agcagtaaaa
1501 gctcttggat tcctgaaaca gaaagccgca gagcagaagg tggcagcatg acaccggaca
1561 ttatcctgca gcgtaccggg atcgatgtga gagctgtcga acagggggaa gatgcgtggc
1621 acaaattacg gctcggcgtc atcaccgctt cagaagttca caacgtgata gcaaaacccc
1681 gctccggaaa gaagtggcct gacatgaaaa tgtcctactt ccacaccctg cttgctgagg
1741 tttgcaccgg tgtggctccg gaagttaacg ctaaagcact ggcctgggga aaacagtacg
1801 agaacgacgc cagaaccctg tttgaattca cttccggcgt gaatgttact gaatccccga
1861 tcatctatcg cgacgaaagt atgcgtaccg cctgctctcc cgatggttta tgcagtgacg
1921 gcaacggcct tgaactgaaa tgcccgttta cctcccggga tttcatgaag ttccggctcg
1981 gtggtttcga ggccataaag tcagcttaca tggcccaggt gcagtacagc atgtgggtga
2041 cgcgaaaaaa tgcctggtac tttgccaact atgacccgcg tatgaagcgt gaaggcctgc
2101 attatgtcgt gattgagcgg gatgaaaagt acatggcgag ttttgacgag atcgtgccgg
2161 agttcatcga aaaaatggac gaggcactgg ctgaaattgg ttttgtattt ggggagcaat
2221 ggcgatgacg catcctcacg ataataagct tcctgctgaa catcaaaggc aagaaaacat
2281 ctgttgtcaa agacagcatc cttgaacaag gacaattaac agttaacaaa taaaaacgca
2341 aaagaaaatg ccgatattga ctaccggaag cagtgtgacc gtgtgcttct caaatgcctg
2401 attcaggctg tctatgtgtg actgttgagc tgtaacaagt tgtctcaggt gttcaatttc
2461 atgttctagt tgctttgttt tactggtttc acctgttcta ttaggtgtta catgctgttc
2521 atctgttaca ttgtcgatct gttcatggtg aacagcttta aatgcaccaa aaactcgtaa
2581 aagctctgat gtatctatct tttttacacc gttttcatct gtgcatatag ctatactgat
2641 ttcgtcagac tcacagtcaa acatgccggt cagttggcct ggtgatggcg ggatcgttgt
2701 atatttcttg acaccttttc ggcaccgccc taaaattctg cgtcctcata atatatgagg
2761 cgatttatta cgtgtttacg aagcaaaagc taaaaccagg agctatttaa tggataagaa
2821 atactcaata ggcttagata tcggcacaaa tagcgtcgga tgggcggtga tcactgatga
2881 atataaggtt ccgtctaaaa agttcaaggt tctgggaaat acagaccgcc acagtatcaa
2941 aaaaaatctt ataggggctc ttttatttga cagtggagag acagcggaag cgactcgtct
3001 caaacggaca gctcgtagaa ggtatacacg tcggaagaat cgtatttgtt atctacagga
3061 gattttttca aatgagatgg cgaaagtaga tgatagtttc tttcatcgac ttgaagagtc
3121 ttttttggtg gaagaagaca agaagcatga acgtcatcct atttttggaa atatagtaga
3181 tgaagttgct tatcatgaga aatatccaac tatctatcat ctgcgaaaaa aattggtaga
3241 ttctactgat aaagcggatt tgcgcttaat ctatttggcc ttagcgcata tgattaagtt
3301 tcgtggtcat tttttgattg agggagattt aaatcctgat aatagtgatg tggacaaact
3361 atttatccag ttggtacaaa cctacaatca attatttgaa gaaaacccta ttaacgcaag
3421 tggagtagat gctaaagcga ttctttctgc acgattgagt aaatcaagac gattagaaaa
3481 tctcattgct cagctccccg gtgagaagaa aaatggctta tttgggaatc tcattgcttt
3541 gtcattgggt ttgaccccta attttaaatc aaattttgat ttggcagaag atgctaaatt
3601 acagctttca aaagatactt acgatgatga tttagataat ttattggcgc aaattggaga
3661 tcaatatgct gatttgtttt tggcagctaa gaatttatca gatgctattt tactttcaga
3721 tatcctaaga gtaaatactg aaataactaa ggctccccta tcagcttcaa tgattaaacg
3781 ctacgatgaa catcatcaag acttgactct tttaaaagct ttagttcgac aacaacttcc
3841 agaaaagtat aaagaaatct tttttgatca atcaaaaaac ggatatgcag gttatattga
3901 tgggggagct agccaagaag aattttataa atttatcaaa ccaattttag aaaaaatgga
3961 tggtactgag gaattattgg tgaaactaaa tcgtgaagat ttgctgcgca agcaacggac
4021 ctttgacaac ggctctattc cccatcaaat tcacttgggt gagctgcatg ctattttgag
4081 aagacaagaa gacttttatc catttttaaa agacaatcgt gagaagattg aaaaaatctt
4141 gacttttcga attccttatt atgttggtcc attggcgcgt ggcaatagtc gttttgcatg
4201 gatgactcgg aagtctgaag aaacaattac cccatggaat tttgaagaag ttgtcgataa
4261 aggtgcttca gctcaatcat ttattgaacg catgacaaac tttgataaaa atcttccaaa
4321 tgaaaaagta ctaccaaaac atagtttgct ttatgagtat tttacggttt ataacgaatt
4381 gacaaaggtc aaatatgtta ctgaaggaat gcgaaaacca gcatttcttt caggtgaaca
4441 gaagaaagcc attgttgatt tactcttcaa aacaaatcga aaagtaaccg ttaagcaatt
4501 aaaagaagat tatttcaaaa aaatagaatg ttttgatagt gttgaaattt caggagttga
4561 agatagattt aatgcttcat taggtaccta ccatgatttg ctaaaaatta ttaaagataa
4621 agattttttg gataatgaag aaaatgaaga tatcttagag gatattgttt taacattgac
4681 cttatttgaa gatagggaga tgattgagga aagacttaaa acatatgctc acctctttga
4741 tgataaggtg atgaaacagc ttaaacgtcg ccgttatact ggttggggac gtttgtctcg
4801 aaaattgatt aatggtatta gggataagca atctggcaaa acaatattag attttttgaa
4861 atcagatggt tttgccaatc gcaattttat gcagctgatc catgatgata gtttgacatt
4921 taaagaagac attcaaaaag cacaagtgtc tggacaaggc gatagtttac atgaacatat
4981 tgcaaattta gctggtagcc ctgctattaa aaaaggtatt ttacagactg taaaagttgt
5041 tgatgaattg gtcaaagtaa tggggcggca taagccagaa aatatcgtta ttgaaatggc
5101 acgtgaaaat cagacaactc aaaagggcca gaaaaattcg cgagagcgta tgaaacgaat
5161 cgaagaaggt atcaaagaat taggaagtca gattcttaaa gagcatcctg ttgaaaatac
5221 tcaattgcaa aatgaaaagc tctatctcta ttatctccaa aatggaagag acatgtatgt
5281 ggaccaagaa ttagatatta atcgtttaag tgattatgat gtcgatcaca ttgttccaca
5341 aagtttcctt aaagacgatt caatagacaa taaggtctta acgcgttctg ataaaaatcg
5401 tggtaaatcg gataacgttc caagtgaaga agtagtcaaa aagatgaaaa actattggag
5461 acaacttcta aacgccaagt taatcactca acgtaagttt gataatttaa cgaaagctga
5521 acgtggaggt ttgagtgaac ttgataaagc tggttttatc aaacgccaat tggttgaaac
5581 tcgccaaatc actaagcatg tggcacaaat tttggatagt cgcatgaata ctaaatacga
5641 tgaaaatgat aaacttattc gagaggttaa agtgattacc ttaaaatcta aattagtttc
5701 tgacttccga aaagatttcc aattctataa agtacgtgag attaacaatt accatcatgc
5761 ccatgatgcg tatctaaatg ccgtcgttgg aactgctttg attaagaaat atccaaaact
5821 tgaatcggag tttgtctatg gtgattataa agtttatgat gttcgtaaaa tgattgctaa
5881 gtctgagcaa gaaataggca aagcaaccgc aaaatatttc ttttactcta atatcatgaa
5941 cttcttcaaa acagaaatta cacttgcaaa tggagagatt cgcaaacgcc ctctaatcga
6001 aactaatggg gaaactggag aaattgtctg ggataaaggg cgagattttg ccacagtgcg
6061 caaagtattg tccatgcccc aagtcaatat tgtcaagaaa acagaagtac agacaggcgg
6121 attctccaag gagtcaattt taccaaaaag aaattcggac aagcttattg ctcgtaaaaa
6181 agactgggat ccaaaaaaat atggtggttt tgatagtcca acggtagctt attcagtcct
6241 agtggttgct aaggtggaaa aagggaaatc gaagaagtta aaatccgtta aagagttact
6301 agggatcaca attatggaaa gaagttcctt tgaaaaaaat ccgattgact ttttagaagc
6361 taaaggatat aaggaagtta aaaaagactt aatcattaaa ctacctaaat atagtctttt
6421 tgagttagaa aacggtcgta aacggatgct ggctagtgcc ggagaattac aaaaaggaaa
6481 tgagctggct ctgccaagca aatatgtgaa ttttttatat ttagctagtc attatgaaaa
6541 gttgaagggt agtccagaag ataacgaaca aaaacaattg tttgtggagc agcataagca
6601 ttatttagat gagattattg agcaaatcag tgaattttct aagcgtgtta ttttagcaga
6661 tgccaattta gataaagttc ttagtgcata taacaaacat agagacaaac caatacgtga
6721 acaagcagaa aatattattc atttatttac gttgacgaat cttggagctc ccgctgcttt
6781 taaatatttt gatacaacaa ttgatcgtaa acgatatacg tctacaaaag aagttttaga
6841 tgccactctt atccatcaat ccatcactgg tctttatgaa acacgcattg atttgagtca
6901 gctaggaggt gactgatggc tggttggcgt actgttgtgg taaataccca tatggacagt
6961 tttccctttg atatgtaacg gtgaacagtt gttctacttt tgtttgttag tcttgatgct
7021 tcactgatag atacaagagc cataagaacc tcagatcctt ccgtatttag ccagtatgtt
7081 ctctagtgtg gttcgttgtt tttgcgtgag ccatgagaac gaaccattga gatcatactt
7141 actttgcatg tcactcaaaa attttgcctc aaaactggtg agctgaattt ttgcagttaa
7201 agcatcgtgt agtgtttttc ttagtccgtt atgtaggtag gaatctgatg taatggttgt
7261 tggtattttg tcaccattca tttttatctg gttgttctca agttcggtta cgagatccat
7321 ttgtctatct agttcaactt ggaaaatcaa cgtatcagtc gggcggcctc gcttatcaac
7381 caccaatttc atattgctgt aagtgtttaa atctttactt attggtttca aaacccattg
7441 gttaagcctt ttaaactcat ggtagttatt ttcaagcatt aacatgaact taaattcatc
7501 aaggctaatc tctatatttg ccttgtgagt tttcttttgt gttagttctt ttaataacca
7561 ctcataaatc ctcatagagt atttgttttc aaaagactta acatgttcca gattatattt
7621 tatgaatttt tttaactgga aaagataagg caatatctct tcactaaaaa ctaattctaa
7681 tttttcgctt gagaacttgg catagtttgt ccactggaaa atctcaaagc ctttaaccaa
7741 aggattcctg atttccacag ttctcgtcat cagctctctg gttgctttag ctaatacacc
7801 ataagcattt tccctactga tgttcatcat ctgaacgtat tggttataag tgaacgatac
7861 cgtccgttct ttccttgtag ggttttcaat cgtggggttg agtagtgcca cacagcataa
7921 aattagcttg gtttcatgct ccgttaagtc atagcgacta atcgctagtt catttgcttt
7981 gaaaacaact aattcagaca tacatctcaa ttggtctagg tgattttaat cactatacca
8041 attgagatgg gctagtcaat gataattact agtccttttc ctttgagttg tgggtatctg
8101 taaattctgc tagacctttg ctggaaaact tgtaaattct gctagaccct ctgtaaattc
8161 cgctagacct ttgtgtgttt tttttgttta tattcaagtg gttataattt atagaataaa
8221 gaaagaataa aaaaagataa aaagaataga tcccagccct gtgtataact cactacttta
8281 gtcagttccg cagtattaca aaaggatgtc gcaaacgctg tttgctcctc tacaaaacag
8341 accttaaaac cctaaaggct taagtagcac cctcgcaagc tcgggcaaat cgctgaatat
8401 tccttttgtc tccgaccatc aggcacctga gtcgctgtct ttttcgtgac attcagttcg
8461 ctgcgctcac ggctctggca gtgaatgggg gtaaatggca ctacaggcgc cttttatgga
8521 ttcatgcaag gaaactaccc ataatacaag aaaagcccgt cacgggcttc tcagggcgtt
8581 ttatggcggg tctgctatgt ggtgctatct gactttttgc tgttcagcag ttcctgccct
8641 ctgattttcc agtctgacca cttcggatta tcccgtgaca ggtcattcag actggctaat
8701 gcacccagta aggcagcggt atcatcaaca ggcttacccg tcttactgtc ggggatcgac
8761 gctctccctt atgcgactcc tgcacctttc gtcttcgaat aaatacctgt gacggaagat
8821 cacttcgcag aataaataaa tcctggtgtc cctgttgata ccgggaagcc ctgggccaac
8881 ttttggcgaa aatgagacgt tgatcggcac gtaagaggtt ccaactttca ccataatgaa
8941 ataagatcac taccgggcgt attttttgag ttatcgagat tttcaggagc taaggaagct
9001 aaaatggaga aaaaaatcac tggatatacc accgttgata tatcccaatg gcatcgtaaa
9061 gaacattttg aggcatttca gtcagttgct caatgtacct ataaccagac cgttcagctg
9121 ggtcatccac cggatcaatt cccctgctcg cgcaggctgg gtgccaagct ctcgggtaac
9181 atcaaggccc gatccttgga gcccttgccc tcccgcacga tgatcgtgcc gtgatcgaaa
9241 tccagatcct tgacccgcag ttgcaaaccc tcactgatcc ggctcacggt aactgatgcc
9301 gtatttgcag taccagcgta cggcccacag aatgatgtca cgctgaaaat gccggccttt
9361 gaatgggttc atgtgcagct ccatcagcaa aaggggatga taagtttatc accaccgact
9421 atttgcaaca gtgccgttga tcgtgctatg atcgactgat gtcatcagcg gtggagtgca
9481 atgtcgtgca atacgaatgg cgaaaagccg agctcatcgg tcagcttctc aaccttgggg
9541 ttacccccgg cggtgtgctg ctggtccaca gctccttccg tagcgtccgg cccctcgaag
9601 atgggccact tggactgatc gaggccctgc gtgctgcgct gggtccggga gggacgctcg
9661 tcatgccctc gtggtcaggt ctggacgacg agccgttcga tcctgccacg tcgcccgtta
9721 caccggacct tggagttgtc tctgacacat tctggcgcct gccaaatgta aagcgcagcg
9781 cccatccatt tgcctttgcg gcagcggggc cacaggcaga gcagatcatc tctgatccat
9841 tgcccctgcc acctcactcg cctgcaagcc cggtcgcccg tgtccatgaa ctcgatgggc
9901 aggtacttct cctcggcgtg ggacacgatg ccaacacgac gctgcatctt gccgagttga
9961 tggcaaaggt tccctatggg gtgccgagac actgcaccat tcttcaggat ggcaagttgg
10021 tacgcgtcga ttatctcgag aatgaccact gctgtgagcg ctttgccttg gcggacaggt
10081 ggctcaagga gaagagcctt cagaaggaag gtccagtcgg tcatgccttt gctcggttga
10141 tccgctcccg cgacattgtg gcgacagccc tgggtcaact gggccgagat ccgttgatct
10201 tcctgcatcc gccagaggcg ggatgcgaag aatgcgatgc cgctcgccag tcgattggct
10261 gagctcatga gcggagaacg agatgacgtt ggaggggcaa ggtcgcgctg attgctgggg
10321 caacacgtga aaggcgagat caccaaggta gtcggcaaat aatgtctaac ggtacccggg
10381 gatcctctag agtcgacctg cataatgtgc ctgtcaaatg gacgaagcag ggattctgca
10441 aaccctatgc tactccgtca agccgtcaat tgtctgattc gttaccaatt atgacaactt
10501 gacggctaca tcattcactt tttcttcaca accggcacgg aactcgctcg ggctggcccc
10561 ggtgcatttt ttaaataccc gcgagaaata gagttgatcg tcaaaaccaa cattgcgacc
10621 gacggtggcg ataggcatcc gggtggtgct caaaagcagc ttcgcctggc tgatacgttg
10681 gtcctcgcgc cagcttaaga cgctaatccc taactgctgg cggaaaagat gtgacagacg
10741 cgacggcgac aagcaaacat gctgtgcgac gctggcgata tcaaaattgc tgtctgccag
10801 gtgatcgctg atgtactgac aagcctcgcg tacccgatta tccatcggtg gatggagcga
10861 ctcgttaatc gcttccatgc gccgcagtaa caattgctca agcagattta tcgccagcag
10921 ctccgaatag cgcccttccc cttgcccggc gttaatgatt tgcccaaaca ggtcgctgaa
10981 atgcggctgg tgcgcttcat ccgggcgaaa gaaccccgta ttggcaaata ttgacggcca
11041 gttaagccat tcatgccagt aggcgcgcgg acgaaagtaa acccactggt gataccattc
11101 gcgagcctcc ggatgacgac cgtagtgatg aatctctcct ggcgggaaca gcaaaatatc
11161 acccggtcgg caaacaaatt ctcgtccctg atttttcacc accccctgac cgcgaatggt
11221 gagattgaga atataacctt tcattcccag cggtcggtcg ataaaaaaat cgagataacc
11281 gttggcctca atcggcgtta aacccgccac cagatgggca ttaaacgagt atcccggcag
11341 caggggatca ttttgcgctt cagccatact tttcatactc ccgccattca gag
//
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