| 质粒名称: | pAAV.hSynap.iGABASnFR.F102G |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 112160 |
| 存储实验室: | Loren Looger |
|
载体骨架基本信息 |
|
| 载体名称: | pAAV.hSynapsin |
| 载体出品公司: | -- |
| 载体抗性: | 氨苄青霉素 |
| 空载体大小: | -- |
| 完整质粒大小: | -- |
| 载体修饰: | -- |
| 载体类型: | AAV |
| 筛选标记: | -- |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | iGABASnFR.F102G |
| 物种来源: | -- |
| 插入大小: | -- |
| 基因突变: | F102G |
| GenBank编号 | -- |
| 启动子 | hSynapsin |
| 标签/融合蛋白: | -- |
| 克隆方法: | 限制性内切酶 |
| 5' 克隆位点: | -- |
| 3' 克隆位点: | -- |
| 5' 测序引物 | -- |
| 3' 测序引物 | -- |
|
文献引用方法 |
|
| 材料和方法部分: | pAAV.hSynap.iGABASnFR.F102G was a gift from Loren Looger (Addgene plasmid # 112160 ; http://n2t.net/addgene:112160 ; RRID:Addgene_112160) |
| 参考文献部分: | A genetically encoded fluorescent sensor for in vivo imaging of GABA. Marvin JS, Shimoda Y, Magloire V, Leite M, Kawashima T, Jensen TP, Kolb I, Knott EL, Novak O, Podgorski K, Leidenheimer NJ, Rusakov DA, Ahrens MB, Kullmann DM, Looger LL. Nat Methods. 2019 Aug;16(8):763-770. doi: 10.1038/s41592-019-0471-2. Epub 2019 Jul 15. 10.1038/s41592-019-0471-2 PubMed 31308547 |
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pAAV.hSynap.iGABASnFR.F102G载体质粒基本信息
pAAV.hSynap.iGABASnFR.F102G质粒图谱载体图谱和pAAV.hSynap.iGABASnFR.F102G载体序列质粒序列多克隆位点信息
pAAV.hSynap.iGABASnFR.F102G质粒载体简介
pAAV.hSynap.iGABASnFR.F102G质粒序列载体序列
LOCUS pAAV.hSynap.iGABASnFR.F102G 6312 bp ds-DNA circular SYN 12-MAY-2021
DEFINITION GABA Sensor.
ACCESSION .
VERSION .
KEYWORDS pAAV.hSynap.iGABASnFR.F102G
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6312)
AUTHORS Marvin JS, Shimoda Y, Magloire V, Leite M, Kawashima T, Jensen TP,
Kolb I, Knott EL, Novak O, Podgorski K, Leidenheimer NJ, Rusakov DA,
Ahrens MB, Kullmann DM, Looger LL
TITLE A genetically encoded fluorescent sensor for in vivo imaging of
GABA.
JOURNAL Nat Methods. 2019 Aug;16(8):763-770. doi: 10.1038/s41592-019-0471-2.
Epub 2019 Jul 15.
PUBMED 31308547
REFERENCE 2 (bases 1 to 6312)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..6312
/organism="synthetic DNA construct"
/mol_type="other DNA"
primer_bind complement(65..82)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(236..824)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(316..335)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(995..1855)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 1618..1637
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(1856..1960)
/gene="bla"
/label=AmpR promoter
rep_origin complement(1986..2441)
/direction=LEFT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(2123..2144)
/label=F1ori-F
/note="F1 origin, forward primer"
primer_bind 2335..2354
/label=F1ori-R
/note="F1 origin, reverse primer"
repeat_region 2622..2751
/label=AAV2 ITR
promoter 2827..3274
/label=hSyn promoter
/note="human synapsin I promoter; confers neuron-specific
expression (Kugler et al., 2003)"
regulatory 3303..3312
/regulatory_class="other"
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
sig_peptide 3309..3371
/product="leader sequence from mouse immunoglobulin kappa
light chain"
/label=Ig-kappa leader
primer_bind 4415..4436
/label=EGFP-C
/note="EGFP, forward primer"
primer_bind complement(4539..4560)
/label=EGFP-N
/note="EGFP, reverse primer"
CDS 5067..5096
/codon_start=1
/product="Myc (human c-Myc proto-oncogene) epitope tag"
/label=Myc
/translation="EQKLISEEDL"
CDS 5100..5246
/codon_start=1
/product="transmembrane domain from platelet derived growth
factor receptor beta"
/label=PDGFR-beta TM domain
/translation="AVGQDTQEVIVVPHSLPFKVVVISAILALVVLTIISLIILIMLWQ
KKPR"
misc_feature 5262..5850
/label=WPRE
/note="woodchuck hepatitis virus posttranscriptional
regulatory element"
primer_bind complement(5315..5335)
/label=WPRE-R
/note="WPRE, reverse primer"
CDS complement(5733..5744)
/codon_start=1
/product="Factor Xa recognition and cleavage site"
/label=Factor Xa site
/translation="IEGR"
primer_bind complement(5853..5869)
/label=KS primer
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(5854..5870)
/label=pBluescriptKS
/note="For pBluescript vector"
polyA_signal 5899..6020
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(5910..5929)
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
primer_bind 5964..5983
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
repeat_region 6182..6311
/label=AAV2 ITR
ORIGIN
1 tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg
61 cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc
121 actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt
181 gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc
241 ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa
301 acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc
361 ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg
421 cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc
481 tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc
541 gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca
601 ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact
661 acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg
721 gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt
781 ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct
841 tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga
901 gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa
961 tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac
1021 ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga
1081 taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc
1141 cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca
1201 gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta
1261 gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg
1321 tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc
1381 gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg
1441 ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt
1501 ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt
1561 cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata
1621 ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc
1681 gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac
1741 ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa
1801 ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct
1861 tcctttttca atattattga agcatttatc agggttattg tctcatgagc ggatacatat
1921 ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc
1981 cacctaaatt gtaagcgtta atattttgtt aaaattcgcg ttaaattttt gttaaatcag
2041 ctcatttttt aaccaatagg ccgaaatcgg caaaatccct tataaatcaa aagaatagac
2101 cgagataggg ttgagtgttg ttccagtttg gaacaagagt ccactattaa agaacgtgga
2161 ctccaacgtc aaagggcgaa aaaccgtcta tcagggcgat ggcccactac gtgaaccatc
2221 accctaatca agttttttgg ggtcgaggtg ccgtaaagca ctaaatcgga accctaaagg
2281 gagcccccga tttagagctt gacggggaaa gccggcgaac gtggcgagaa aggaagggaa
2341 gaaagcgaaa ggagcgggcg ctagggcgct ggcaagtgta gcggtcacgc tgcgcgtaac
2401 caccacaccc gccgcgctta atgcgccgct acagggcgcg tcccattcgc cattcaggct
2461 gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc agctgcgcgc
2521 tcgctcgctc actgaggccg cccgggcaaa gcccgggcgt cgggcgacct ttggtcgccc
2581 ggcctcagtg atcggtgcgg gcctcttcgc tattacgcca gctgcgcgct cgctcgctca
2641 ctgaggccgc ccgggcaaag cccgggcgtc gggcgacctt tggtcgcccg gcctcagtga
2701 gcgagcgagc gcgcagagag ggagtggcca actccatcac taggggttcc ttgtagttaa
2761 tgattaaccc gccatgctac ttatctacgt agccatgctc taggaagatc cagggccctg
2821 cgtatgagtg caagtgggtt ttaggaccag gatgaggcgg ggtgggggtg cctacctgac
2881 gaccggcccc gacccactgg acaagcaccc aacccccatt ccccaaattg cgcatcccct
2941 atcagagagg gggaggggaa acaggatgcg gcgaggcgcg tgcgcactgc cagcttcagc
3001 accgcggaca gtgccttcgc ccccgcctgg cggcgcgcgc caccgccgcc tcagcactga
3061 aggcgcgctg acgtcactcg ccggtccccc gcaaactccc cttcccggcc accttggtcg
3121 cgtccgcgcc gccgccggcc cagccggacc gcaccacgcg aggcgcgaga taggggggca
3181 cgggcgcgac catctgcgct gcggcgccgg cgactcagcg ctgcctcagt ctgcggtggg
3241 cagcggagga gtcgtgtcgt gcctgagagc gcagtccaat tcaagctgct agcaaggatc
3301 ccgccaccat ggagacagac acactcctgc tatgggtact gctgctctgg gttccaggtt
3361 ccactggtga cagatctgaa agcatcaatt tcgtgagctg gggcggtagc acccaggatg
3421 cgcagaagca ggcctgggcc gacccgttca gcaaggccag cggcattacc gtggtccagg
3481 atgggcccac cgactacggc aaactcaagg ccatggtcga aagcggcaac gtgcagtggg
3541 acgtggtgga tgtagaggcc gacttcgcct tgcgcgccgc cgccgaaggc ctgctcgaac
3601 ccctggattt ctcggtgatc cagcgcgaca agatcgaccc gcgcttcgtc tccgaccatg
3661 gcgtgggctc gttcctgggt tctttcgtgc tcggctacaa cgaaggcaag ctcggagcca
3721 gcaagcccca ggactggacc gcgctgttcg acaccaagac ctaccccggc aaacgcgccc
3781 tctacaaatg gccgagccct ggcgtgctcg aactggccct gctcgccgac ggcgtaccgg
3841 ccgacaagct ctacccgctg gacctggacc gcgccttcaa gaaactcgac accatcaaga
3901 aagacatcgt ctggtggggc ggcggtgcac agtcgcagca gctgctggcc tccggcgaag
3961 tcagcatggg ccagttctgg aacggtcgca tccacgccct gcaagaagac ggcgcgccag
4021 tgggcgtgag ctggaagcag aacctggtga tggccgacat cctcgtcgtg cctaaaggca
4081 cgaagaacaa agccgccgcg atgaagttcc tggccagtgc cagcagcgcc aaaggccagg
4141 acgacttttc cgccctgacc gcctatgctc cagtgaacat cgacagtgtg cagcgcctcg
4201 accttgcgca ggtccggatc accgccgaca agcagaagaa cggcatcatg gcgaacttca
4261 agatccgcca caacgtggag gacggcagcg tgcagctcgc cgaccactac cagcagaaca
4321 cccccatcgg cgacggcccc gtgctgctgc ccgacaacca ctacctgagc acccagtccg
4381 tgctgagcaa agaccccaac gagaagcgcg atcacatggt cctgctggag ttcgtgaccg
4441 ccgccgggat cactctcggc atggacgagc tgtacaaggg cggtaccgga gggagcatga
4501 gcaagggcga ggagctgttc accggggtgg tgcccatcct ggtcgagctg gacggcgacg
4561 taaacggcca caagttcagc gtgcgcggcg agggcgaggg cgatgccacc aacggcaagc
4621 tgaccctgaa gttcatctgc accaccggca agctgcccgt gccctggccc accctcgtga
4681 ccaccctgac ctacggcgtg cagtgcttca gccgctaccc cgaccacatg aagcagcacg
4741 acttcttcaa gtccgccatg cccgaaggct acgtccagga gcgcaccatc agcttcaagg
4801 acgacggcac ctacaagacc cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc
4861 gcatcgagct gaagggcatc gacttcaagg aggacggcaa catcctgggg cacaagctgg
4921 agtacaactg gaacgccaac ctggcaccga acctgccgac cgcctacgtc aaggatcaga
4981 tcaccctcga tttcgcctac tgggccaaga acggtccggc cattgcgaca cggtggaatg
5041 aatggctagt caaactgcag gtcgacgaac aaaaactcat ctcagaagag gatctgaatg
5101 ctgtgggcca ggacacgcag gaggtcatcg tggtgccaca ctccttgccc tttaaggtgg
5161 tggtgatctc agccatcctg gccctggtgg tgctcaccat catctccctt atcatcctca
5221 tcatgctttg gcagaagaag ccacgttaga agcttatcga taatcaacct ctggattaca
5281 aaatttgtga aagattgact ggtattctta actatgttgc tccttttacg ctatgtggat
5341 acgctgcttt aatgcctttg tatcatgcta ttgcttcccg tatggctttc attttctcct
5401 ccttgtataa atcctggttg ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac
5461 gtggcgtggt gtgcactgtg tttgctgacg caacccccac tggttggggc attgccacca
5521 cctgtcagct cctttccggg actttcgctt tccccctccc tattgccacg gcggaactca
5581 tcgccgcctg ccttgcccgc tgctggacag gggctcggct gttgggcact gacaattccg
5641 tggtgttgtc ggggaaatca tcgtcctttc cttggctgct cgcctgtgtt gccacctgga
5701 ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct caatccagcg gaccttcctt
5761 cccgcggcct gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga
5821 gtcggatctc cctttgggcc gcctccccgc atcgataccg tcgacctcga cccgggcggc
5881 cgcttcgagc agacatgata agatacattg atgagtttgg acaaaccaca actagaatgc
5941 agtgaaaaaa atgctttatt tgtgaaattt gtgatgctat tgctttattt gtaaccatta
6001 taagctgcaa taaacaagtt aacaacaaca attgcattca ttttatgttt caggttcagg
6061 gggagatgtg ggaggttttt taaagcaagt aaaacctcta caaatgtggt aaaatcgata
6121 aggatcttcc tagagcatgg ctacgtagat aagtagcatg gcgggttaat cattaactac
6181 aaggaacccc tagtgatgga gttggccact ccctctctgc gcgctcgctc gctcactgag
6241 gccgggcgac caaaggtcgc ccgacgcccg ggctttgccc gggcggcctc agtgagcgag
6301 cgagcgcgca gc
//
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