| 质粒名称: | pAAV.hSynap.iGABASnFR |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 112159 |
| 存储实验室: | Loren Looger |
|
载体骨架基本信息 |
|
| 载体名称: | pAAV.hSynapsin |
| 载体出品公司: | -- |
| 载体抗性: | 氨苄青霉素 |
| 空载体大小: | -- |
| 完整质粒大小: | -- |
| 载体修饰: | -- |
| 载体类型: | AAV |
| 筛选标记: | -- |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | iGABASnFR |
| 物种来源: | -- |
| 插入大小: | -- |
| 基因突变: | -- |
| GenBank编号 | -- |
| 启动子 | hSynapsin |
| 标签/融合蛋白: | -- |
| 克隆方法: | 限制性内切酶 |
| 5' 克隆位点: | -- |
| 3' 克隆位点: | -- |
| 5' 测序引物 | -- |
| 3' 测序引物 | -- |
|
文献引用方法 |
|
| 材料和方法部分: | pAAV.hSynap.iGABASnFR was a gift from Loren Looger (Addgene plasmid # 112159 ; http://n2t.net/addgene:112159 ; RRID:Addgene_112159) |
| 参考文献部分: | A genetically encoded fluorescent sensor for in vivo imaging of GABA. Marvin JS, Shimoda Y, Magloire V, Leite M, Kawashima T, Jensen TP, Kolb I, Knott EL, Novak O, Podgorski K, Leidenheimer NJ, Rusakov DA, Ahrens MB, Kullmann DM, Looger LL. Nat Methods. 2019 Aug;16(8):763-770. doi: 10.1038/s41592-019-0471-2. Epub 2019 Jul 15. 10.1038/s41592-019-0471-2 PubMed 31308547 |
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pAAV.hSynap.iGABASnFR载体质粒基本信息
pAAV.hSynap.iGABASnFR质粒图谱载体图谱和pAAV.hSynap.iGABASnFR载体序列质粒序列多克隆位点信息
pAAV.hSynap.iGABASnFR质粒载体简介
pAAV.hSynap.iGABASnFR质粒序列载体序列
LOCUS pAAV.hSynap.iGABASnFR 6302 bp ds-DNA circular SYN 12-MAY-2021
DEFINITION GABA Sensor.
ACCESSION .
VERSION .
KEYWORDS pAAV.hSynap.iGABASnFR
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6302)
AUTHORS Marvin JS, Shimoda Y, Magloire V, Leite M, Kawashima T, Jensen TP,
Kolb I, Knott EL, Novak O, Podgorski K, Leidenheimer NJ, Rusakov DA,
Ahrens MB, Kullmann DM, Looger LL
TITLE A genetically encoded fluorescent sensor for in vivo imaging of
GABA.
JOURNAL Nat Methods. 2019 Aug;16(8):763-770. doi: 10.1038/s41592-019-0471-2.
Epub 2019 Jul 15.
PUBMED 31308547
REFERENCE 2 (bases 1 to 6302)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..6302
/organism="synthetic DNA construct"
/mol_type="other DNA"
primer_bind complement(66..83)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(237..825)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(317..336)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(996..1856)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 1619..1638
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(1857..1961)
/gene="bla"
/label=AmpR promoter
rep_origin complement(1987..2442)
/direction=LEFT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(2124..2145)
/label=F1ori-F
/note="F1 origin, forward primer"
primer_bind 2336..2355
/label=F1ori-R
/note="F1 origin, reverse primer"
repeat_region 2686..2815
/label=AAV2 ITR
promoter 2891..3338
/label=hSyn promoter
/note="human synapsin I promoter; confers neuron-specific
expression (Kugler et al., 2003)"
regulatory 3367..3376
/regulatory_class="other"
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
sig_peptide 3373..3435
/product="leader sequence from mouse immunoglobulin kappa
light chain"
/label=Ig-kappa leader
primer_bind 4479..4500
/label=EGFP-C
/note="EGFP, forward primer"
primer_bind complement(4603..4624)
/label=EGFP-N
/note="EGFP, reverse primer"
CDS 5131..5160
/codon_start=1
/product="Myc (human c-Myc proto-oncogene) epitope tag"
/label=Myc
/translation="EQKLISEEDL"
CDS 5164..5310
/codon_start=1
/product="transmembrane domain from platelet derived growth
factor receptor beta"
/label=PDGFR-beta TM domain
/translation="AVGQDTQEVIVVPHSLPFKVVVISAILALVVLTIISLIILIMLWQ
KKPR"
misc_feature 5326..5914
/label=WPRE
/note="woodchuck hepatitis virus posttranscriptional
regulatory element"
primer_bind complement(5379..5399)
/label=WPRE-R
/note="WPRE, reverse primer"
CDS complement(5797..5808)
/codon_start=1
/product="Factor Xa recognition and cleavage site"
/label=Factor Xa site
/translation="IEGR"
primer_bind complement(5917..5933)
/label=KS primer
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(5918..5934)
/label=pBluescriptKS
/note="For pBluescript vector"
polyA_signal 5963..6084
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(5974..5993)
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
primer_bind 6028..6047
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
ORIGIN
1 ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc
61 gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct
121 cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg
181 tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc
241 cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga
301 aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct
361 cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg
421 gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag
481 ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat
541 cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac
601 aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac
661 tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc
721 ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt
781 tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc
841 ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg
901 agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca
961 atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca
1021 cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag
1081 ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac
1141 ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc
1201 agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct
1261 agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc
1321 gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg
1381 cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc
1441 gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat
1501 tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag
1561 tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat
1621 aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg
1681 cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca
1741 cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga
1801 aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc
1861 ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag cggatacata
1921 tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg
1981 ccacctaaat tgtaagcgtt aatattttgt taaaattcgc gttaaatttt tgttaaatca
2041 gctcattttt taaccaatag gccgaaatcg gcaaaatccc ttataaatca aaagaataga
2101 ccgagatagg gttgagtgtt gttccagttt ggaacaagag tccactatta aagaacgtgg
2161 actccaacgt caaagggcga aaaaccgtct atcagggcga tggcccacta cgtgaaccat
2221 caccctaatc aagttttttg gggtcgaggt gccgtaaagc actaaatcgg aaccctaaag
2281 ggagcccccg atttagagct tgacggggaa agccggcgaa cgtggcgaga aaggaaggga
2341 agaaagcgaa aggagcgggc gctagggcgc tggcaagtgt agcggtcacg ctgcgcgtaa
2401 ccaccacacc cgccgcgctt aatgcgccgc tacagggcgc gtcccattcg ccattcaggc
2461 tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc gctattacgc cagctgcgcg
2521 ctcgctcgct cactgaggcc gcgcgggagt agggtgacgc cgcggsgaac ccccacccca
2581 cccgccgcgc ttaatgcgcc gctacagggc gcgtcccatt cgccattcag gctgcgcaac
2641 tgttgggaag ggcgatcggt gcgggcctct tcgctattac gccagctgcg cgctcgctcg
2701 ctcactgagg ccgcccgggc aaagcccggg cgtcgggcga cctttggtcg cccggcctca
2761 gtgagcgagc gagcgcgcag agagggagtg gccaactcca tcactagggg ttccttgtag
2821 ttaatgatta acccgccatg ctacttatct acgtagccat gctctaggaa gatccagggc
2881 cctgcgtatg agtgcaagtg ggttttagga ccaggatgag gcggggtggg ggtgcctacc
2941 tgacgaccgg ccccgaccca ctggacaagc acccaacccc cattccccaa attgcgcatc
3001 ccctatcaga gagggggagg ggaaacagga tgcggcgagg cgcgtgcgca ctgccagctt
3061 cagcaccgcg gacagtgcct tcgcccccgc ctggcggcgc gcgccaccgc cgcctcagca
3121 ctgaaggcgc gctgacgtca ctcgccggtc ccccgcaaac tccccttccc ggccaccttg
3181 gtcgcgtccg cgccgccgcc ggcccagccg gaccgcacca cgcgaggcgc gagatagggg
3241 ggcacgggcg cgaccatctg cgctgcggcg ccggcgactc agcgctgcct cagtctgcgg
3301 tgggcagcgg aggagtcgtg tcgtgcctga gagcgcagtc caattcaagc tgctagcaag
3361 gatcccgcca ccatggagac agacacactc ctgctatggg tactgctgct ctgggttcca
3421 ggttccactg gtgacagatc tgaaagcatc aatttcgtga gctggggcgg tagcacccag
3481 gatgcgcaga agcaggcctg ggccgacccg ttcagcaagg ccagcggcat taccgtggtc
3541 caggatgggc ccaccgacta cggcaaactc aaggccatgg tcgaaagcgg caacgtgcag
3601 tgggacgtgg tggatgtaga ggccgacttc gccttgcgcg ccgccgccga aggcctgctc
3661 gaacccctgg atttctcggt gatccagcgc gacaagatcg acccgcgctt cgtctccgac
3721 catggcgtgg gctcgttcct gttctctttc gtgctcggct acaacgaagg caagctcgga
3781 gccagcaagc cccaggactg gaccgcgctg ttcgacacca agacctaccc cggcaaacgc
3841 gccctctaca aatggccgag ccctggcgtg ctcgaactgg ccctgctcgc cgacggcgta
3901 ccggccgaca agctctaccc gctggacctg gaccgcgcct tcaagaaact cgacaccatc
3961 aagaaagaca tcgtctggtg gggcggcggt gcacagtcgc agcagctgct ggcctccggc
4021 gaagtcagca tgggccagtt ctggaacggt cgcatccacg ccctgcaaga agacggcgcg
4081 ccagtgggcg tgagctggaa gcagaacctg gtgatggccg acatcctcgt cgtgcctaaa
4141 ggcacgaaga acaaagccgc cgcgatgaag ttcctggcca gtgccagcag cgccaaaggc
4201 caggacgact tttccgccct gaccgcctat gctccagtga acatcgacag tgtgcagcgc
4261 ctcgaccttg cgcaggtccg gatcaccgcc gacaagcaga agaacggcat catggcgaac
4321 ttcaagatcc gccacaacgt ggaggacggc agcgtgcagc tcgccgacca ctaccagcag
4381 aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca accactacct gagcacccag
4441 tccgtgctga gcaaagaccc caacgagaag cgcgatcaca tggtcctgct ggagttcgtg
4501 accgccgccg ggatcactct cggcatggac gagctgtaca agggcggtac cggagggagc
4561 atgagcaagg gcgaggagct gttcaccggg gtggtgccca tcctggtcga gctggacggc
4621 gacgtaaacg gccacaagtt cagcgtgcgc ggcgagggcg agggcgatgc caccaacggc
4681 aagctgaccc tgaagttcat ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc
4741 gtgaccaccc tgacctacgg cgtgcagtgc ttcagccgct accccgacca catgaagcag
4801 cacgacttct tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac catcagcttc
4861 aaggacgacg gcacctacaa gacccgcgcc gaggtgaagt tcgagggcga caccctggtg
4921 aaccgcatcg agctgaaggg catcgacttc aaggaggacg gcaacatcct ggggcacaag
4981 ctggagtaca actggaacgc caacctggca ccgaacctgc cgaccgccta cgtcaaggat
5041 cagatcaccc tcgatttcgc ctactgggcc aagaacggtc cggccattgc gacacggtgg
5101 aatgaatggc tagtcaaact gcaggtcgac gaacaaaaac tcatctcaga agaggatctg
5161 aatgctgtgg gccaggacac gcaggaggtc atcgtggtgc cacactcctt gccctttaag
5221 gtggtggtga tctcagccat cctggccctg gtggtgctca ccatcatctc ccttatcatc
5281 ctcatcatgc tttggcagaa gaagccacgt tagaagctta tcgataatca acctctggat
5341 tacaaaattt gtgaaagatt gactggtatt cttaactatg ttgctccttt tacgctatgt
5401 ggatacgctg ctttaatgcc tttgtatcat gctattgctt cccgtatggc tttcattttc
5461 tcctccttgt ataaatcctg gttgctgtct ctttatgagg agttgtggcc cgttgtcagg
5521 caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg gggcattgcc
5581 accacctgtc agctcctttc cgggactttc gctttccccc tccctattgc cacggcggaa
5641 ctcatcgccg cctgccttgc ccgctgctgg acaggggctc ggctgttggg cactgacaat
5701 tccgtggtgt tgtcggggaa atcatcgtcc tttccttggc tgctcgcctg tgttgccacc
5761 tggattctgc gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc agcggacctt
5821 ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag
5881 acgagtcgga tctccctttg ggccgcctcc ccgcatcgat accgtcgacc tcgacccggg
5941 cggccgcttc gagcagacat gataagatac attgatgagt ttggacaaac cacaactaga
6001 atgcagtgaa aaaaatgctt tatttgtgaa atttgtgatg ctattgcttt atttgtaacc
6061 attataagct gcaataaaca agttaacaac aacaattgca ttcattttat gtttcaggtt
6121 cagggggaga tgtgggaggt tttttaaagc aagtaaaacc tctacaaatg tggtaaaatc
6181 gataaggatc ttcctagagc atggctacgt agataagtag catggcgggt taatcattaa
6241 ctacaaggaa cccctagtga tggagttggc cactccctct ctgcgcgctc gcgcgctcgc
6301 ag
//
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