| 质粒名称: | p31sheLL |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 37322 |
| 存储实验室: | John Schiller |
|
载体骨架基本信息 |
|
| 载体名称: | custom |
| 载体出品公司: | -- |
| 载体抗性: | 氨苄青霉素 |
| 空载体大小: | -- |
| 完整质粒大小: | -- |
| 载体修饰: | -- |
| 载体类型: | 哺乳细胞表达 |
| 筛选标记: | -- |
| 高拷贝/低拷贝: | -- |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | HPV31L1+L2,p31LLw |
| 物种来源: | H. sapiens (human) |
| 插入大小: | -- |
| 基因突变: | -- |
| GenBank编号 | -- |
| 启动子 | CMV |
| 标签/融合蛋白: | -- |
| 克隆方法: | -- |
| 5' 克隆位点: | -- |
| 3' 克隆位点: | -- |
| 5' 测序引物 | -- |
| 3' 测序引物 | -- |
|
文献引用方法 |
|
| 材料和方法部分: | p31sheLL was a gift from John Schiller (Addgene plasmid # 37322 ; http://n2t.net/addgene:37322 ; RRID:Addgene_37322) |
| 参考文献部分: | Genital transmission of HPV in a mouse model is potentiated by nonoxynol-9 and inhibited by carrageenan. Roberts JN, Buck CB, Thompson CD, Kines R, Bernardo M, Choyke PL, Lowy DR, Schiller JT. Nat Med. 2007 Jul;13(7):857-61. Epub 2007 Jul 1. 10.1038/nm1598 PubMed 17603495 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS p31sheLL 10729 bp ds-DNA circular SYN 12-MAY-2021
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS p31sheLL
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 10729)
AUTHORS Roberts JN, Buck CB, Thompson CD, Kines R, Bernardo M, Choyke PL,
Lowy DR, Schiller JT
TITLE Genital transmission of HPV in a mouse model is potentiated by
nonoxynol-9 and inhibited by carrageenan.
JOURNAL Nat Med. 2007 Jul;13(7):857-61. Epub 2007 Jul 1.
PUBMED 17603495
REFERENCE 2 (bases 1 to 10729)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..10729
/organism="synthetic DNA construct"
/mol_type="other DNA"
misc_feature 3666..4254
/label=WPRE
/note="woodchuck hepatitis virus posttranscriptional
regulatory element"
primer_bind complement(3719..3739)
/label=WPRE-R
/note="WPRE, reverse primer"
CDS complement(4137..4148)
/codon_start=1
/product="Factor Xa recognition and cleavage site"
/label=Factor Xa site
/translation="IEGR"
primer_bind complement(4291..4308)
/label=BGH-rev
/note="Bovine growth hormone terminator, reverse primer.
Also called BGH reverse"
polyA_signal 4297..4521
/label=bGH poly(A) signal
/note="bovine growth hormone polyadenylation signal"
rep_origin 4567..4995
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(4654..4673)
/label=F1ori-R
/note="F1 origin, reverse primer"
primer_bind 4864..4885
/label=F1ori-F
/note="F1 origin, forward primer"
primer_bind complement(5004..5024)
/label=pBABE 3'
/note="SV40 enhancer, reverse primer for pBABE vectors"
CDS 6953..7321
/codon_start=1
/gene="Sh ble from Streptoalloteichus hindustanus"
/product="antibiotic-binding protein"
/label=BleoR
/note="confers resistance to bleomycin, phleomycin, and
Zeocin(TM)"
/translation="KLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDDVT
LFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGREF
ALRDPAGNCVHFVAEEQD"
polyA_signal 7451..7572
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(7488..7507)
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind 7542..7561
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
primer_bind complement(7621..7637)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(7621..7637)
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind complement(7634..7656)
/label=M13/pUC Reverse
/note="In lacZ gene"
protein_bind 7645..7661
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(7669..7699)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 7714..7735
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
primer_bind complement(7852..7869)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(8023..8608)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(8103..8122)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(8779..9639)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 9402..9421
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(9640..9744)
/gene="bla"
/label=AmpR promoter
primer_bind complement(9819..9838)
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
enhancer 10010..10389
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 10390..10593
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind 10544..10564
/label=CMV-F
/note="Human CMV immediate early promoter, forward primer"
protein_bind 10595..10613
/gene="tetO"
/label=tet operator
/bound_moiety="tetracycline repressor TetR"
/note="bacterial operator O2 for the tetR and tetA genes"
protein_bind 10616..10634
/gene="tetO"
/label=tet operator
/bound_moiety="tetracycline repressor TetR"
/note="bacterial operator O2 for the tetR and tetA genes"
primer_bind 10644..10668
/label=LNCX
/note="Human CMV promoter, forward primer"
ORIGIN
1 tccggagggc cgcgccacca tgagcctgtg gaggcccagc gaggccaccg tgtacctgcc
61 ccccgtgccc gtgagcaagg tggtgagcac cgacgagtac gtgaccagga ccaacatcta
121 ctaccacgcc ggcagcgcca ggctgctgac cgtgggccac ccctactaca gcatccccaa
181 gagcgacaac cccaagaaga tcgtggtgcc caaggtgagc ggcctgcagt acagggtgtt
241 cagggtgagg ctgcccgacc ccaacaagtt cggcttcccc gacaccagct tctacaaccc
301 cgagacccag aggctggtgt gggcctgcgt gggcctggag gtgggcaggg gccagcccct
361 gggcgtgggc atcagcggcc accccctgct gaacaagttc gacgacaccg agaacagcaa
421 caggtacgcc ggcggccccg gcaccgacaa cagggagtgc atcagcatgg actacaagca
481 gacccagctg tgcctgctgg gctgcaagcc ccccatcggc gagcactggg gcaagggcag
541 cccctgcagc aacaacgcca tcacccccgg cgactgcccc cccctggagc tgaagaacag
601 cgtgatccag gacggcgaca tggtggacac cggcttcggc gccatggact tcaccgccct
661 gcaggacacc aagagcaacg tgcccctgga catctgcaac agcatctgca agtaccccga
721 ctacctgaag atggtggccg agccctacgg cgacaccctg ttcttctacc tgaggaggga
781 gcagatgttc gtgaggcact tcttcaacag gagcggcacc gtgggcgaga gcgtgcccac
841 cgacctgtac atcaagggca gcggcagcac cgccaccctg gccaacagca cctacttccc
901 cacccccagc ggcagcatgg tgaccagcga cgcccagatc ttcaacaagc cctactggat
961 gcagagggcc cagggccaca acaacggcat ctgctggggc aaccagctgt tcgtgaccgt
1021 ggtggacacc accaggagca ccaacatgag cgtgtgcgcc gccatcgcca acagcgacac
1081 caccttcaag agcagcaact tcaaggagta cctgaggcac ggcgaggagt tcgacctgca
1141 gttcatcttc cagctgtgca agatcaccct gagcgccgac atcatgacct acatccacag
1201 catgaacccc gccatcctgg aggactggaa cttcggcctg accacccccc ccagcggcag
1261 cctggaggac acctacaggt tcgtgaccag ccaggccatc acctgccaga agaccgcccc
1321 ccagaagccc aaggaggacc ccttcaagga ctacgtgttc tgggaggtga acctgaagga
1381 gaagttcagc gccgacctgg accagttccc cctgggcagg aagttcctgc tgcaggccgg
1441 ctacagggcc aggcccaagt tcaaggccgg caagaggagc gcccccagcg ccagcaccac
1501 cacccccgcc aagaggaaga agaccaagaa gtaaaagctt tttgaattct ttggatccac
1561 tagggctcga ggaagcttaa aacagctctg gggttgtacc caccccagag gcccacgtgg
1621 cggctagtac tccggtattg cggtaccctt gtacgcctgt tttatactcc cttcccgtaa
1681 cttagacgca caaaaccaag ttcaatagaa gggggtacaa accagtacca ccacgaacaa
1741 gcacttctgt ttccccggtg atgtcgtata gactgcttgc gtggttgaaa gcgacggatc
1801 cgttatccgc ttatgtactt cgagaagccc agtaccacct cggaatcttc gatgcgttgc
1861 gctcagcact caaccccaga gtgtagctta ggctgatgag tctggacatc cctcaccggt
1921 gacggtggtc caggctgcgt tggcggccta cctatggcta acgccatggg acgctagttg
1981 tgaacaaggt gtgaagagcc tattgagcta cataagaatc ctccggcccc tgaatgcggc
2041 taatcccaac ctcggagcag gtggtcacaa accagtgatt ggcctgtcgt aacgcgcaag
2101 tccgtggcgg aaccgactac tttgggtgtc cgtgtttcct tttattttat tgtggctgct
2161 tatggtgaca atcacagatt gttatcataa agcgaattgg attgcggccg cgccaccatg
2221 aggagcaaga ggagcaccaa gaggaccaag agggccagcg ccacccagct gtaccagacc
2281 tgcaaggccg ccggcacctg ccccagcgac gtgatcccca agatcgagca caccaccatc
2341 gccgaccaga tcctgaggta cggcagcatg ggcgtgttct tcggcggcct gggcatcggc
2401 agcggcagcg gcaccggcgg caggaccggc tacgtgcccc tgagcaccag gcccagcacc
2461 gtgagcgagg ccagcatccc catcaggccc cccgtgagca tcgaccccgt gggccccctg
2521 gaccccagca tcgtgagcct ggtggaggag agcggcatcg tggacgtggg cgcccccgcc
2581 cccatccccc acccccccac caccagcggc ttcgacatcg ccaccaccgc cgacaccacc
2641 cccgccatcc tggacgtgac cagcgtgagc acccacgaga accccacctt caccgacccc
2701 agcgtgctgc agccccccac ccccgccgag accagcggcc acctgctgct gagcagcagc
2761 agcatcagca cccacaacta cgaggagatc cccatggaca ccttcatcgt gagcaccaac
2821 aacgagaaca tcaccagcag cacccccatc cccggcgtga ggaggcccgc caggctgggc
2881 ctgtacagca aggccaccca gcaggtgaag gtgatcgacc ccaccttcct gagcgccccc
2941 aagcagctga tcacctacga gaaccccgcc tacgagaccg tgaacgccga ggagagcctg
3001 tacttcagca acaccagcca caacatcgcc cccgaccccg acttcctgga catcatcgcc
3061 ctgcacaggc ccgccctgac cagcaggagg aacaccgtga ggtacagcag gctgggcaac
3121 aagcagaccc tgaggaccag gagcggcgcc accatcggcg ccagggtgca ctactactac
3181 gacatcagca gcatcaaccc cgccggcgag agcatcgaga tgcagcccct gggcgccagc
3241 gccaccacca ccagcaccct gaacgacggc ctgtacgaca tctacgccga caccgacttc
3301 accgtggaca cccccgccac ccacaacgtg agccccagca ccgccgtgca gagcaccagc
3361 gccgtgagcg cctacgtgcc caccaacacc accgtgcccc tgagcaccgg cttcgacatc
3421 cccatcttca gcggccccga cgtgcccatc gagcacgccc ccacccaggt gttccccttc
3481 cccctggccc ccaccacccc ccaggtgagc atcttcgtgg acggcggcga cttctacctg
3541 caccccagct actacatgct gaagaggagg aggaagaggg tgagctactt cttcaccgac
3601 gtgagcgtgg ccgcctgaaa gcttttgaat tctttggatc cactagcgat atcaagctta
3661 tcgataatca acctctggat tacaaaattt gtgaaagatt gactggtatt cttaactatg
3721 ttgctccttt tacgctatgt ggatacgctg ctttaatgcc tttgtatcat gctattgctt
3781 cccgtatggc tttcattttc tcctccttgt ataaatcctg gttgctgtct ctttatgagg
3841 agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc
3901 ccactggttg gggcattgcc accacctgtc agctcctttc cgggactttc gctttccccc
3961 tccctattgc cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg acaggggctc
4021 ggctgttggg cactgacaat tccgtggtgt tgtcggggaa atcatcgtcc tttccttggc
4081 tgctcgcctg tgttgccacc tggattctgc gcgggacgtc cttctgctac gtcccttcgg
4141 ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc
4201 gtcttcgcct tcgccctcag acgagtcgga tctccctttg ggccgcctcc ccgcatcgat
4261 accgtcggcc cgtttaaacc cgctgatcag cctcgactgt gccttctagt tgccagccat
4321 ctgttgtttg cccctccccc gtgccttcct tgaccctgga aggtgccact cccactgtcc
4381 tttcctaata aaatgaggaa attgcatcgc attgtctgag taggtgtcat tctattctgg
4441 ggggtggggt ggggcaggac agcaaggggg aggattggga agacaatagc aggcatgctg
4501 gggatgcggt gggctctatg gcttctgagg cggaaagaac cagctggggc tctagggggt
4561 atccccacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg
4621 tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc
4681 tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc
4741 gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat ggttcacgta
4801 gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta
4861 atagtggact cttgttccaa actggaacaa cactcaaccc tatctcggtc tattcttttg
4921 atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg atttaacaaa
4981 aatttaacgc gaattaattc tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg
5041 ctccccagca ggcagaagta tgcaaagcat gcagaattct atcaaatatt taaagaaaaa
5101 aaaattgtat caactttcta caatctcttt cagaagacag aagcagaggg aatacttcct
5161 aaatcattca actaggccag cattacctta ataccggaac tagaaaatga cattacaaga
5221 aaagaaaaca acagaccaat atctctcatg aacaaagata caaacatttt caacaaaata
5281 ttagcaaaaa gaatccaaga atgtatcaaa aaatatacac cacaaccaag tagaatttat
5341 tccagatatg taagggtggt tcaacgtttg aaaatcaatt aacgtaattt gtcccatcaa
5401 caggttaaag aagaaaatca catggtcata ttgatagaca cagaaaaagc atttgacaaa
5461 atttaacacc cattcatgat gcaatctctc agtaaactag gaatagagga aaacttcctc
5521 agcttgaatg taccttcctc tcaattttgc tatgaacctg aaactcctct taaaaaataa
5581 agtttttcat ttaaaaagaa aacaaaaaac atggaggagc gttgatgtat ctcattttag
5641 accaatcagc tatggatagt taggcgacag cacagatagc tgctgtactt ctgtttctgg
5701 caatgttcca gactacattt aaaaaatttt taattataga cttgtactta atgttcaaga
5761 aaaatatgaa aatggctttg ccgtgttaat gctactcttt tttaaaaaaa actaaagttc
5821 aaactttatt tatatttcat tagtttttta gctactgttc tttttctgtt ctgggatctc
5881 attcagaatg ccacattaca tataattctc atgtctcctt gggttcctct tagttttgac
5941 agttcctcag acttttctta tttttgatga ccttgacagt tttgaggagt actggttaga
6001 tatagggtaa tggtttttaa agtatatttg tcatgattta tactggggta agggtttggg
6061 gaggaagccc atggggtaaa gtactgttct catcacatca tatcaaggtt atataccatc
6121 aatattgcca cagatgttac ttagcctttt aatatttctc taatttagtg tatatgcaat
6181 gatagttctc tgatttctga gattgagttt ctcatgtgta atgattattt agagtttctc
6241 tttcatctgt tcaaattttt gtctagtttt attttttact gatttgtaag acttcttttt
6301 ataatctgca tattacaatt ctctttactg gggtgttgca aatattttct gtcattctat
6361 ggcctgactt ttcttaatgg ttttttaatt ttaaaaataa gtcttaatat tcatgcaatc
6421 taattaacaa tcttttcttt gtggttagga ctttgagtca taagaaattt ttctctacac
6481 tgaagtcatg atggcatgct tctatattat tttctaaaag atttaaagtt ttgccttctc
6541 catttagact tataattcac tggaattttt ttgtgtgtat ggtatgacat atgggttccc
6601 ttttattttt tacatataaa tatatttccc tgtttttcta aaaaagaaaa agatcatcat
6661 tttcccattg taaaatgcca tatttttttc ataggtcact tacatatatc aatgggtctg
6721 tttctgagct ctactctatt ttatcagcct cactgtctat ccccacacat ctcatgcttt
6781 gctctaaatc ttgatattta gtggaacatt ctttcccatt ttgttctaca agaatatttt
6841 tgttattgtc ttttgggctt ctatatacat tttagaatga ggttggcaag ttaacaaaca
6901 gcttttttgg ggtgaacata ttgactacaa atttatgtgg aaagaaagta ccaagttgac
6961 cagtgccgtt ccggtgctca ccgcgcgcga cgtcgccgga gcggtcgagt tctggaccga
7021 ccggctcggg ttctcccggg acttcgtgga ggacgacttc gccggtgtgg tccgggacga
7081 cgtgaccctg ttcatcagcg cggtccagga ccaggtggtg ccggacaaca ccctggcctg
7141 ggtgtgggtg cgcggcctgg acgagctgta cgccgagtgg tcggaggtcg tgtccacgaa
7201 cttccgggac gcctccgggc cggccatgac cgagatcggc gagcagccgt gggggcggga
7261 gttcgccctg cgcgacccgg ccggcaactg cgtgcacttc gtggccgagg agcaggactg
7321 acacgtgcta cgagatttcg attccaccgc cgccttctat gaaaggttgg gcttcggaat
7381 cgttttccgg gacgccggct ggatgatcct ccagcgcggg gatctcatgc tggagttctt
7441 cgcccacccc aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac
7501 aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat
7561 caatgtatct tatcatgtct gtataccgtc gacctctagc tagagcttgg cgtaatcatg
7621 gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc
7681 cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc
7741 gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat
7801 cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac
7861 tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt
7921 aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca
7981 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc
8041 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact
8101 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct
8161 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag
8221 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca
8281 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa
8341 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc
8401 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag
8461 aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg
8521 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ttttttgttt gcaagcagca
8581 gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga
8641 cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat
8701 cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga
8761 gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg
8821 tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga
8881 gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc
8941 agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac
9001 tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc
9061 agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc
9121 gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc
9181 catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt
9241 ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc
9301 atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg
9361 tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag
9421 cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat
9481 cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc
9541 atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa
9601 aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta
9661 ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa
9721 aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtcgacgg
9781 atcgggagat ctcccgatcc cctatggtgc actctcagta caatctgctc tgatgccgca
9841 tagttaagcc agtatctgct ccctgcttgt gtgttggagg tcgctgagta gtgcgcgagc
9901 aaaatttaag ctacaacaag gcaaggcttg accgacaatt gcatgaagaa tctgcttagg
9961 gttaggcgtt ttgcgctgct tcgcgatgta cgggccagat atacgcgttg acattgatta
10021 ttgactagtt attaatagta atcaattacg gggtcattag ttcatagccc atatatggag
10081 ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc
10141 ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac tttccattga
10201 cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat
10261 atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc
10321 cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct
10381 attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca
10441 cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg gaaccaaaat
10501 caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg
10561 cgtgtacggt gggaggtcta tataagcaga gctctcccta tcagtgatag agatctccct
10621 atcagtgata gagatcgtcg acgagctcgt ttagtgaacc gtcagatcgc ctggagacgc
10681 catccacgct gttttgacct ccatagaaga caccgggacc gatccagcc
//
