Yes2C-A-bk1

LOCUS       Yes2C-A-bk1       6061 bp DNA     circular SYN 09-APR-2026
DEFINITION  pYES2C.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6061)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Sep 30, 2026 from SnapGene 6.0.2
            https://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..6061
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        261..702
                     /gene="S. cerevisiae GAL1"
                     /label=GAL1 promoter
                     /note="inducible promoter, regulated by Gal4"
     protein_bind    261..378
                     /label=UAS
                     /bound_moiety="Gal4"
                     /note="upstream activating sequence mediating 
                     Gal4-dependent induction"
     promoter        734..752
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             781..798
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             802..834
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             838..861
                     /codon_start=1
                     /product="Xpress(TM) epitope tag, including an enterokinase
                     recognition and cleavage site"
                     /label=Xpress(TM) tag
                     /translation="DLYDDDDK"
     CDS             967..1008
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             1018..1035
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     terminator      1069..1316
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     rep_origin      complement(1564..2152)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2323..3183)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     CDS             complement(3279..4082)
                     /codon_start=1
                     /gene="S. cerevisiae URA3"
                     /product="orotidine-5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=URA3
                     /note="yeast auxotrophic marker, counterselectable with 
                     5-fluoroorotic acid (5-FOA)"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
     promoter        complement(4083..4298)
                     /gene="S. cerevisiae URA3"
                     /label=URA3 promoter
     rep_origin      4899..5776
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     rep_origin      complement(join(5845..6061,1..239))
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
ORIGIN
        1 cccactacgt gaaccatcac cctaatcaag ttttttgggg tcgaggtgcc gtaaagcact
       61 aaatcggaac cctaaaggga gcccccgatt tagagcttga cggggaaagc cggcgaacgt
      121 ggcgagaaag gaagggaaga aagcgaaagg agcgggcgct agggcgctgg caagtgtagc
      181 ggtcacgctg cgcgtaacca ccacacccgc cgcgcttaat gcgccgctac agggcgcgtg
      241 gggatcgatc cactagtcca cggattagaa gccgccgagc gggtgacagc cctccgaagg
      301 aagactctcc tccgtgcgtc ctcgtcttca ccggtcgcgt tcctgaaacg cagatgtgcc
      361 tcgcgccgca ctgctccgaa caataaagat tctacaatac tagcttttat ggttatgaag
      421 aggaaaaatt ggcagtaacc tggccccaca aaccttcaaa tgaacgaatc aaattaacaa
      481 ccataggatg ataatgcgat tagtttttta gccttatttc tggggtaatt aatcagcgaa
      541 gcgatgattt ttgatctatt aacagatata taaatgcaaa aactgcataa ccactttaac
      601 taatactttc aacattttcg gtttgtatta cttcttattc aaatgtaata aaagtatcaa
      661 caaaaaattg ttaatatacc tctatacttt aacgtcaagg agaaaaaacc ccggatcgga
      721 ctactagcag ctgtaatacg actcactata gggaatatta agcttaccat ggggggttct
      781 catcatcatc atcatcatgg tatggctagc atgactggtg gacagcaaat gggtcgggat
      841 ctgtacgacg atgacgataa ggtcgactct cgaggatccg gtacccggta tcgatttaaa
      901 agtgtggtgg aattctgcag atatccagca cagtggcggc cgctcgagtc tagagggccc
      961 ttcgaaggta agcctatccc taaccctctc ctcggtctcg attctacgcg taccggtcat
     1021 catcaccatc accattgagt ttaaacccgc tgatcctaga gggccgcatc atgtaattag
     1081 ttatgtcacg cttacattca cgccctcccc ccacatccgc tctaaccgaa aaggaaggag
     1141 ttagacaacc tgaagtctag gtccctattt atttttttat agttatgtta gtattaagaa
     1201 cgttatttat atttcaaatt tttctttttt ttctgtacag acgcgtgtac gcatgtaaca
     1261 ttatactgaa aaccttgctt gagaaggttt tgggacgctc gaaggcttta atttgcaagc
     1321 tgcggccctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg
     1381 ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt
     1441 atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa
     1501 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc
     1561 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag
     1621 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt
     1681 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg
     1741 aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg
     1801 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg
     1861 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac
     1921 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg
     1981 gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt
     2041 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg
     2101 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc
     2161 tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt
     2221 ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt
     2281 taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag
     2341 tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt
     2401 cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg caatgatacc
     2461 gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc
     2521 cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg
     2581 ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac
     2641 aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg
     2701 atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc
     2761 tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact
     2821 gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc
     2881 aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat
     2941 acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc
     3001 ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac
     3061 tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa
     3121 aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact
     3181 catactcttc ctttttcaat gggtaataac tgatataatt aaattgaagc tctaatttgt
     3241 gagtttagta tacatgcatt tacttataat acagtttttt agttttgctg gccgcatctt
     3301 ctcaaatatg cttcccagcc tgcttttctg taacgttcac cctctacctt agcatccctt
     3361 ccctttgcaa atagtcctct tccaacaata ataatgtcag atcctgtaga gaccacatca
     3421 tccacggttc tatactgttg acccaatgcg tctcccttgt catctaaacc cacaccgggt
     3481 gtcataatca accaatcgta accttcatct cttccaccca tgtctctttg agcaataaag
     3541 ccgataacaa aatctttgtc gctcttcgca atgtcaacag tacccttagt atattctcca
     3601 gtagataggg agcccttgca tgacaattct gctaacatca aaaggcctct aggttccttt
     3661 gttacttctt ctgccgcctg cttcaaaccg ctaacaatac ctgggcccac cacaccgtgt
     3721 gcattcgtaa tgtctgccca ttctgctatt ctgtatacac ccgcagagta ctgcaatttg
     3781 actgtattac caatgtcagc aaattttctg tcttcgaaga gtaaaaaatt gtacttggcg
     3841 gataatgcct ttagcggctt aactgtgccc tccatggaaa aatcagtcaa gatatccaca
     3901 tgtgttttta gtaaacaaat tttgggacct aatgcttcaa ctaactccag taattccttg
     3961 gtggtacgaa catccaatga agcacacaag tttgtttgct tttcgtgcat gatattaaat
     4021 agcttggcag caacaggact aggatgagta gcagcacgtt ccttatatgt agctttcgac
     4081 atgatttatc ttcgtttcct gcaggttttt gttctgtgca gttgggttaa gaatactggg
     4141 caatttcatg tttcttcaac actacatatg cgtatatata ccaatctaag tctgtgctcc
     4201 ttccttcgtt cttccttctg ttcggagatt accgaatcaa aaaaatttca aggaaaccga
     4261 aatcaaaaaa aagaataaaa aaaaaatgat gaattgaaaa gctagcttat cgatgataag
     4321 ctgtcaaaca tgagaattaa ttccacggac tatagactat acctagtata ctccgtctac
     4381 tgtacgatac acttccgctc aggtccttgt cctttaacga ggccttacca ctcttttgtt
     4441 actctattga tccagctcag caaaggcagt gtgatctaag attctatctt cgcgatgtag
     4501 taaaactagc tagaccgaga aagagactag aaatgcaaaa ggcacttcta caatggctgc
     4561 catcattatt atccgatgtg acgctgcagc ttctcaatga tattcgaata cgctttgagg
     4621 agatacagcc taatatccga caaactgttt tacagattta cgatcgtact tgttacccat
     4681 cattgaattt tgaacatccg aacctgggag ttttccctga aacagatagt atatttgaac
     4741 ctgtataata atatatagtc tagcgcttta cggaagacaa tgtatgtatt tcggttcctg
     4801 gagaaactat tgcatctatt gcataggtaa tcttgcacgt cgcatccccg gttcattttc
     4861 tgcgtttcca tcttgcactt caatagcata tctttgttaa cgaagcatct gtgcttcatt
     4921 ttgtagaaca aaaatgcaac gcgagagcgc taatttttca aacaaagaat ctgagctgca
     4981 tttttacaga acagaaatgc aacgcgaaag cgctatttta ccaacgaaga atctgtgctt
     5041 catttttgta aaacaaaaat gcaacgcgag agcgctaatt tttcaaacaa agaatctgag
     5101 ctgcattttt acagaacaga aatgcaacgc gagagcgcta ttttaccaac aaagaatcta
     5161 tacttctttt ttgttctaca aaaatgcatc ccgagagcgc tatttttcta acaaagcatc
     5221 ttagattact ttttttctcc tttgtgcgct ctataatgca gtctcttgat aactttttgc
     5281 actgtaggtc cgttaaggtt agaagaaggc tactttggtg tctattttct cttccataaa
     5341 aaaagcctga ctccacttcc cgcgtttact gattactagc gaagctgcgg gtgcattttt
     5401 tcaagataaa ggcatccccg attatattct ataccgatgt ggattgcgca tactttgtga
     5461 acagaaagtg atagcgttga tgattcttca ttggtcagaa aattatgaac ggtttcttct
     5521 attttgtctc tatatactac gtataggaaa tgtttacatt ttcgtattgt tttcgattca
     5581 ctctatgaat agttcttact acaatttttt tgtctaaaga gtaatactag agataaacat
     5641 aaaaaatgta gaggtcgagt ttagatgcaa gttcaaggag cgaaaggtgg atgggtaggt
     5701 tatataggga tatagcacag agatatatag caaagagata cttttgagca atgtttgtgg
     5761 aagcggtatt cgcaatggga agctccaccc cggttgataa tcagaaaagc cccaaaaaca
     5821 ggaagattgt ataagcaaat atttaaattg taagcgttaa tattttgtta aaattcgcgt
     5881 taaatttttg ttaaatcagc tcatttttta accaataggc cgaaatcggc aaaatccctt
     5941 ataaatcaaa agaatagacc gagatagggt tgagtgttgt tccagtttgg aacaagagtc
     6001 cactattaaa gaacgtggac tccaacgtca aagggcgaaa aaccgtctat cagggcgatg
     6061 g
//