| 质粒名称: | Yes2C-B-bk1 |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0002150 |
| 产品用途: | Yeast heterologous expression |
| 存储实验室: | Chen Caiyan Lab (陈彩艳实验室) |
|
载体骨架基本信息 |
|
| 载体名称: | Yes2C |
| 表达系统: | 酵母表达 |
| 启动子: | GAL1 |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5alpha |
|
插入基因信息 |
|
| 插入基因名称: | - |
| 物种来源: | NA |
| 密码子优化: | 未优化 |
Yes2C-B-bk1
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
买家导航
Yes2C-B-bk1载体质粒基本信息
Yes2C-B-bk1质粒图谱载体图谱和Yes2C-B-bk1载体序列质粒序列多克隆位点信息
Yes2C-B-bk1质粒载体简介
Yes2C-B-bk1质粒序列载体序列
LOCUS Yes2C-B-bk1 6062 bp DNA circular SYN 09-APR-2026
DEFINITION pYES2C.
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6062)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Sep 30, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..6062
/mol_type="other DNA"
/organism="synthetic DNA construct"
promoter 261..702
/gene="S. cerevisiae GAL1"
/label=GAL1 promoter
/note="inducible promoter, regulated by Gal4"
protein_bind 261..378
/label=UAS
/bound_moiety="Gal4"
/note="upstream activating sequence mediating
Gal4-dependent induction"
promoter 734..752
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
CDS 781..798
/codon_start=1
/product="6xHis affinity tag"
/label=6xHis
/translation="HHHHHH"
CDS 802..834
/codon_start=1
/product="leader peptide from bacteriophage T7 gene 10"
/label=T7 tag (gene 10 leader)
/note="promotes efficient translation in E. coli"
/translation="MASMTGGQQMG"
CDS 838..861
/codon_start=1
/product="Xpress(TM) epitope tag, including an enterokinase
recognition and cleavage site"
/label=Xpress(TM) tag
/translation="DLYDDDDK"
CDS 968..1009
/codon_start=1
/product="epitope tag from simian virus 5"
/label=V5 tag
/translation="GKPIPNPLLGLDST"
CDS 1019..1036
/codon_start=1
/product="6xHis affinity tag"
/label=6xHis
/translation="HHHHHH"
terminator 1070..1317
/gene="S. cerevisiae CYC1"
/label=CYC1 terminator
/note="transcription terminator for CYC1"
rep_origin complement(1565..2153)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(2324..3184)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
CDS complement(3280..4083)
/codon_start=1
/gene="S. cerevisiae URA3"
/product="orotidine-5'-phosphate decarboxylase, required
for uracil biosynthesis"
/label=URA3
/note="yeast auxotrophic marker, counterselectable with
5-fluoroorotic acid (5-FOA)"
/translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
promoter complement(4084..4299)
/gene="S. cerevisiae URA3"
/label=URA3 promoter
rep_origin 4900..5777
/label=2u ori
/note="yeast 2u plasmid origin of replication"
rep_origin complement(join(5846..6062,1..239))
/direction=LEFT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
ORIGIN
1 cccactacgt gaaccatcac cctaatcaag ttttttgggg tcgaggtgcc gtaaagcact
61 aaatcggaac cctaaaggga gcccccgatt tagagcttga cggggaaagc cggcgaacgt
121 ggcgagaaag gaagggaaga aagcgaaagg agcgggcgct agggcgctgg caagtgtagc
181 ggtcacgctg cgcgtaacca ccacacccgc cgcgcttaat gcgccgctac agggcgcgtg
241 gggatcgatc cactagtcca cggattagaa gccgccgagc gggtgacagc cctccgaagg
301 aagactctcc tccgtgcgtc ctcgtcttca ccggtcgcgt tcctgaaacg cagatgtgcc
361 tcgcgccgca ctgctccgaa caataaagat tctacaatac tagcttttat ggttatgaag
421 aggaaaaatt ggcagtaacc tggccccaca aaccttcaaa tgaacgaatc aaattaacaa
481 ccataggatg ataatgcgat tagtttttta gccttatttc tggggtaatt aatcagcgaa
541 gcgatgattt ttgatctatt aacagatata taaatgcaaa aactgcataa ccactttaac
601 taatactttc aacattttcg gtttgtatta cttcttattc aaatgtaata aaagtatcaa
661 caaaaaattg ttaatatacc tctatacttt aacgtcaagg agaaaaaacc ccggatcgga
721 ctactagcag ctgtaatacg actcactata gggaatatta agcttaccat ggggggttct
781 catcatcatc atcatcatgg tatggctagc atgactggtg gacagcaaat gggtcgggat
841 ctgtacgacg atgacgataa gggtcgactc tcgaggatcc ggtacccggt atcgatttaa
901 aagtgtggtg gaattctgca gatatccagc acagtggcgg ccgctcgagt ctagagggcc
961 cttcgaaggt aagcctatcc ctaaccctct cctcggtctc gattctacgc gtaccggtca
1021 tcatcaccat caccattgag tttaaacccg ctgatcctag agggccgcat catgtaatta
1081 gttatgtcac gcttacattc acgccctccc cccacatccg ctctaaccga aaaggaagga
1141 gttagacaac ctgaagtcta ggtccctatt tattttttta tagttatgtt agtattaaga
1201 acgttattta tatttcaaat ttttcttttt tttctgtaca gacgcgtgta cgcatgtaac
1261 attatactga aaaccttgct tgagaaggtt ttgggacgct cgaaggcttt aatttgcaag
1321 ctgcggccct gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc
1381 gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg
1441 tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa
1501 agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg
1561 cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga
1621 ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg
1681 tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg
1741 gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc
1801 gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg
1861 gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca
1921 ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt
1981 ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag
2041 ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg
2101 gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc
2161 ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt
2221 tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt
2281 ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca
2341 gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg
2401 tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac
2461 cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg
2521 ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc
2581 gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta
2641 caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac
2701 gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc
2761 ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac
2821 tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact
2881 caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa
2941 tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt
3001 cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca
3061 ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa
3121 aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac
3181 tcatactctt cctttttcaa tgggtaataa ctgatataat taaattgaag ctctaatttg
3241 tgagtttagt atacatgcat ttacttataa tacagttttt tagttttgct ggccgcatct
3301 tctcaaatat gcttcccagc ctgcttttct gtaacgttca ccctctacct tagcatccct
3361 tccctttgca aatagtcctc ttccaacaat aataatgtca gatcctgtag agaccacatc
3421 atccacggtt ctatactgtt gacccaatgc gtctcccttg tcatctaaac ccacaccggg
3481 tgtcataatc aaccaatcgt aaccttcatc tcttccaccc atgtctcttt gagcaataaa
3541 gccgataaca aaatctttgt cgctcttcgc aatgtcaaca gtacccttag tatattctcc
3601 agtagatagg gagcccttgc atgacaattc tgctaacatc aaaaggcctc taggttcctt
3661 tgttacttct tctgccgcct gcttcaaacc gctaacaata cctgggccca ccacaccgtg
3721 tgcattcgta atgtctgccc attctgctat tctgtataca cccgcagagt actgcaattt
3781 gactgtatta ccaatgtcag caaattttct gtcttcgaag agtaaaaaat tgtacttggc
3841 ggataatgcc tttagcggct taactgtgcc ctccatggaa aaatcagtca agatatccac
3901 atgtgttttt agtaaacaaa ttttgggacc taatgcttca actaactcca gtaattcctt
3961 ggtggtacga acatccaatg aagcacacaa gtttgtttgc ttttcgtgca tgatattaaa
4021 tagcttggca gcaacaggac taggatgagt agcagcacgt tccttatatg tagctttcga
4081 catgatttat cttcgtttcc tgcaggtttt tgttctgtgc agttgggtta agaatactgg
4141 gcaatttcat gtttcttcaa cactacatat gcgtatatat accaatctaa gtctgtgctc
4201 cttccttcgt tcttccttct gttcggagat taccgaatca aaaaaatttc aaggaaaccg
4261 aaatcaaaaa aaagaataaa aaaaaaatga tgaattgaaa agctagctta tcgatgataa
4321 gctgtcaaac atgagaatta attccacgga ctatagacta tacctagtat actccgtcta
4381 ctgtacgata cacttccgct caggtccttg tcctttaacg aggccttacc actcttttgt
4441 tactctattg atccagctca gcaaaggcag tgtgatctaa gattctatct tcgcgatgta
4501 gtaaaactag ctagaccgag aaagagacta gaaatgcaaa aggcacttct acaatggctg
4561 ccatcattat tatccgatgt gacgctgcag cttctcaatg atattcgaat acgctttgag
4621 gagatacagc ctaatatccg acaaactgtt ttacagattt acgatcgtac ttgttaccca
4681 tcattgaatt ttgaacatcc gaacctggga gttttccctg aaacagatag tatatttgaa
4741 cctgtataat aatatatagt ctagcgcttt acggaagaca atgtatgtat ttcggttcct
4801 ggagaaacta ttgcatctat tgcataggta atcttgcacg tcgcatcccc ggttcatttt
4861 ctgcgtttcc atcttgcact tcaatagcat atctttgtta acgaagcatc tgtgcttcat
4921 tttgtagaac aaaaatgcaa cgcgagagcg ctaatttttc aaacaaagaa tctgagctgc
4981 atttttacag aacagaaatg caacgcgaaa gcgctatttt accaacgaag aatctgtgct
5041 tcatttttgt aaaacaaaaa tgcaacgcga gagcgctaat ttttcaaaca aagaatctga
5101 gctgcatttt tacagaacag aaatgcaacg cgagagcgct attttaccaa caaagaatct
5161 atacttcttt tttgttctac aaaaatgcat cccgagagcg ctatttttct aacaaagcat
5221 cttagattac tttttttctc ctttgtgcgc tctataatgc agtctcttga taactttttg
5281 cactgtaggt ccgttaaggt tagaagaagg ctactttggt gtctattttc tcttccataa
5341 aaaaagcctg actccacttc ccgcgtttac tgattactag cgaagctgcg ggtgcatttt
5401 ttcaagataa aggcatcccc gattatattc tataccgatg tggattgcgc atactttgtg
5461 aacagaaagt gatagcgttg atgattcttc attggtcaga aaattatgaa cggtttcttc
5521 tattttgtct ctatatacta cgtataggaa atgtttacat tttcgtattg ttttcgattc
5581 actctatgaa tagttcttac tacaattttt ttgtctaaag agtaatacta gagataaaca
5641 taaaaaatgt agaggtcgag tttagatgca agttcaagga gcgaaaggtg gatgggtagg
5701 ttatataggg atatagcaca gagatatata gcaaagagat acttttgagc aatgtttgtg
5761 gaagcggtat tcgcaatggg aagctccacc ccggttgata atcagaaaag ccccaaaaac
5821 aggaagattg tataagcaaa tatttaaatt gtaagcgtta atattttgtt aaaattcgcg
5881 ttaaattttt gttaaatcag ctcatttttt aaccaatagg ccgaaatcgg caaaatccct
5941 tataaatcaa aagaatagac cgagataggg ttgagtgttg ttccagtttg gaacaagagt
6001 ccactattaa agaacgtgga ctccaacgtc aaagggcgaa aaaccgtcta tcagggcgat
6061 gg
//
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