pAAV-AscI-CAG-dMito2-mBiyu

LOCUS       pAAV-AscI-CAG-dMito2-mBiyu              5731 bp DNA     circular SYN 12-MAY-2026
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  recombinant plasmid
REFERENCE   1  (bases 1 to 5731)
  AUTHORS   ustc
  TITLE     Direct Submission
  JOURNAL   Exported Sep 15, 2026 from SnapGene 6.0.2
            https://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..5731
                     /mol_type="other DNA"
                     /organism="recombinant plasmid"
     rep_origin      complement(182..770)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(941..1801)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(1802..1906)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(1932..2387)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     misc_feature    2458..2647
                     /label=ITR
     misc_feature    2661..2691
                     /label=AscI-OxE insert
                     /label=AscI-OxE\insert
                     /note="/vntifkey=21"
     misc_feature    2692..2935
                     /label=CMV enhancer
                     /label=CMV\enhancer
                     /note="/vntifkey=21"
     promoter        2942..3219
                     /label=chicken beta-actin promoter
     CDS             3646..3720
                     /codon_start=1
                     /product="mitochondrial presequence of human cytochrome c 
                     oxidase subunit VIII (Rizutto et al., 1989)"
                     /label=cytochrome c oxidase subunit 8A mitochondrial
                     /translation="MSVLTPLLLRGLTGSARRLPVPRAK"
     CDS             3748..3819
                     /codon_start=1
                     /product="mitochondrial presequence of human cytochrome c 
                     oxidase subunit VIII (Rizutto et al., 1989)"
                     /label=COX8 presequence
                     /translation="SVLTPLLLRGLTGSARRLPVPRAK"
     CDS             3859..4605
                     /codon_start=1
                     /label=mBiyu
                     /translation="MVSKGEEDNMASYGALLFREKIPYVVEMEGDVEGMKFSVRGKGHG
                     DANTGKIEASFICTTGELPVPWSSILTTVTYGAQCFAKYPNDIKDYLKSTMPEGYVQER
                     TITFENDGIYKTRAEVTYEKGSVYNRVTLNGSGFKKGGNILGKKLEFNFNASRTYVLPD
                     VENNGIKCYINIRHNVIGGGQIRAEHQQLNTPLGGGPVDIPHYHAIQTHTILSKDPKET
                     RDHMNVVEVFRAIDCKTAYAGMDELYK"
     misc_feature    4633..5221
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             complement(5104..5115)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    5264..5385
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     misc_feature    5488..5677
                     /label=ITR
ORIGIN
        1 cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat
       61 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga
      121 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt
      181 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt
      241 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc
      301 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa
      361 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct
      421 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta
      481 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg
      541 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc
      601 ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta
      661 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg
      721 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt
      781 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg
      841 tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta
      901 aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg
      961 aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg
     1021 tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc
     1081 gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg
     1141 agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg
     1201 aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag
     1261 gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat
     1321 caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc
     1381 cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc
     1441 ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa
     1501 ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac
     1561 gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt
     1621 cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc
     1681 gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa
     1741 caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca
     1801 tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat
     1861 acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa
     1921 aagtgccacc taaattgtaa gcgttaatat tttgttaaaa ttcgcgttaa atttttgtta
     1981 aatcagctca ttttttaacc aataggccga aatcggcaaa atcccttata aatcaaaaga
     2041 atagaccgag atagggttga gtgttgttcc agtttggaac aagagtccac tattaaagaa
     2101 cgtggactcc aacgtcaaag ggcgaaaaac cgtctatcag ggcgatggcc cactacgtga
     2161 accatcaccc taatcaagtt ttttggggtc gaggtgccgt aaagcactaa atcggaaccc
     2221 taaagggagc ccccgattta gagcttgacg gggaaagccg gcgaacgtgg cgagaaagga
     2281 agggaagaaa gcgaaaggag cgggcgctag ggcgctggca agtgtagcgg tcacgctgcg
     2341 cgtaaccacc acacccgccg cgcttaatgc gccgctacag ggcgcgtccc attcgccatt
     2401 caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat tacgccagct
     2461 gcgcgctcgc tcgctcactg aggccgcccg ggcaaagccc gggcgtcggg cgacctttgg
     2521 tcgcccggcc tcagtgagcg agcgagcgcg cagagaggga gtggccaact ccatcactag
     2581 gggttccttg tagttaatga ttaacccgcc atgctactta tctacgtagc catgctctag
     2641 gaagatcgta ggttaattaa ggcgcgcccc taggggagat ggccagccag cccattgacg
     2701 tcaataatga cgtatgttcc catagtaacg ccaataggga ctttccattg acgtcaatgg
     2761 gtggagtatt tacggtaaac tgcccacttg gcagtacatc aagtgtatca tatgccaagt
     2821 acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc ccagtacatg
     2881 accttatggg actttcctac ttggcagtac atctacgtat tagtcatcgc tattaccatg
     2941 gtcgaggtga gccccacgtt ctgcttcact ctccccatct cccccccctc cccaccccca
     3001 attttgtatt tatttatttt ttaattattt tgtgcagcga tgggggcggg gggggggggg
     3061 gggcgcgcgc caggcggggc ggggcggggc gaggggcggg gcggggcgag gcggagaggt
     3121 gcggcggcag ccaatcagag cggcgcgctc cgaaagtttc cttttatggc gaggcggcgg
     3181 cggcggcggc cctataaaaa gcgaagcgcg cggcgggcgg gagtcgctgc gcgctgcctt
     3241 cgccccgtgc cccgctccgc cgccgcctcg cgccgcccgc cccggctctg actgaccgcg
     3301 ttactcccac aggtgagcgg gcgggacggc ccttctcctc cgggctgtaa ttagcgcttg
     3361 gtttaatgac ggcttgtttc ttttctgtgg ctgcgtgaaa gccttgaggg gctccgggag
     3421 ggccctttgt gcggggggag cggctcgggg ctgtccgcgg ggggacggct gccttcgggg
     3481 gggacggggc agggcggggt tcggcttctg gcgtgtgacc ggcggctcta gagcctctgc
     3541 taaccatgtt catgccttct tctttttcct acagctcctg ggcaacgtgc tggttattgt
     3601 gctgtctcat cattttggca aagaattgga tccaccggtg acaccatgtc cgtcctgacg
     3661 ccgctgctgc tgcggggctt gacaggctcg gcccggcggc tcccagtgcc gcgcgccaag
     3721 atccattcgt tggggcctgc aagatccagt gtattaacac ccctgctgct gcggggcttg
     3781 acaggctcgg cccggcggct cccagtgccg cgcgccaaga ttcacagtct tggtccagct
     3841 agatctgcta gcctcgagat ggtgagcaag ggcgaggagg acaacatggc ctcctacgga
     3901 gccctgctgt ttagagagaa gatcccctac gtggtggaga tggagggcga tgtggaggga
     3961 atgaagttct ccgtgagagg caagggccac ggcgatgcta acaccggaaa gatcgaggcc
     4021 agctttatct gcaccaccgg cgagctgccc gtgccttgga gcagcatcct gacaaccgtg
     4081 acatacggcg cccagtgctt cgctaagtac cctaatgata tcaaggacta cctgaagagc
     4141 accatgcccg agggctacgt gcaggaaaga accatcacat ttgagaacga tggcatctac
     4201 aagaccaggg ccgaggtgac ctacgagaag ggaagcgtgt acaacagggt gacactgaac
     4261 ggcagcggct tcaagaaggg cggaaacatc ctgggcaaga agctggagtt caattttaac
     4321 gcctccagga catacgtgct gcctgacgtg gagaacaatg gcatcaagtg ttacatcaac
     4381 atcaggcaca acgtgatcgg cggcggccaa atcagggctg aacaccaaca gctgaatacc
     4441 cccctgggcg gaggacctgt tgacattcca cactaccacg ccatccagac ccacaccatc
     4501 ctgtccaagg accccaagga gacaagggac cacatgaacg tggtggaggt gtttagagcc
     4561 atcgattgca agacagccta cgccggcatg gatgagctgt acaagtagga attcgatatc
     4621 aagcttatcg ataatcaacc tctggattac aaaatttgtg aaagattgac tggtattctt
     4681 aactatgttg ctccttttac gctatgtgga tacgctgctt taatgccttt gtatcatgct
     4741 attgcttccc gtatggcttt cattttctcc tccttgtata aatcctggtt gctgtctctt
     4801 tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac
     4861 gcaaccccca ctggttgggg cattgccacc acctgtcagc tcctttccgg gactttcgct
     4921 ttccccctcc ctattgccac ggcggaactc atcgccgcct gccttgcccg ctgctggaca
     4981 ggggctcggc tgttgggcac tgacaattcc gtggtgttgt cggggaaatc atcgtccttt
     5041 ccttggctgc tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc
     5101 ccttcggccc tcaatccagc ggaccttcct tcccgcggcc tgctgccggc tctgcggcct
     5161 cttccgcgtc ttcgccttcg ccctcagacg agtcggatct ccctttgggc cgcctccccg
     5221 catcgatacc gtcgacccgg gcggccgctt cgagcagaca tgataagata cattgatgag
     5281 tttggacaaa ccacaactag aatgcagtga aaaaaatgct ttatttgtga aatttgtgat
     5341 gctattgctt tatttgtaac cattataagc tgcaataaac aagttaacaa caacaattgc
     5401 attcatttta tgtttcaggt tcagggggag atgtgggagg ttttttaaag caagtaaaac
     5461 ctctacaaat gtggtaaaat cgataaggat cttcctagag catggctacg tagataagta
     5521 gcatggcggg ttaatcatta actacaagga acccctagtg atggagttgg ccactccctc
     5581 tctgcgcgct cgctcgctca ctgaggccgg gcgaccaaag gtcgcccgac gcccgggctt
     5641 tgcccgggcg gcctcagtga gcgagcgagc gcgcagctgc attaatgaat cggccaacgc
     5701 gcggggagag gcggtttgcg tattgggcgc t
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