PABE-4

LOCUS       PABE-4            10342 bp ds-DNA     circular SYN 10-JAN-2019
DEFINITION  Targeted A to G in wheat plants or monocotyledons protoplasts .
ACCESSION   .
VERSION     .
KEYWORDS    PABE-4
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10342)
  AUTHORS   Li C, Zong Y, Wang Y, Jin S, Zhang D, Song Q, Zhang R, Gao C
  TITLE     Expanded base editing in rice and wheat using a Cas9-adenosine 
            deaminase fusion.
  JOURNAL   Genome Biol. 2018 May 29;19(1):59. doi: 10.1186/s13059-018-1443-z.
  PUBMED    29807545
REFERENCE   2  (bases 1 to 10342)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jan 10, 2019 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..10342
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     CDS             836..856
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             869..4969
                     /codon_start=1
                     /product="nickase mutant of the Cas9 endonuclease from the 
                     Streptococcus pyogenes Type II CRISPR/Cas system"
                     /label=Cas9(D10A)
                     /note="generates RNA-guided single strand nicks in DNA due 
                     to the D10A mutation in the RuvC catalytic domain"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     RBS             4882..4890
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     CDS             6164..6211
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     polyA_signal    6269..6445
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     primer_bind     complement(6999..7015)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6999..7015)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7012..7034)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7023..7039
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(7047..7077)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7092..7113
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7230..7247)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7401..7989)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7481..7500)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(8160..9020)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8783..8802
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9021..9125)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ttgccagtgt ttctctttgg ggaatcctgg gatggctcta gccgttccgc agacgggatc
       61 gatttcatga ttttttttgt ttcgttgcat agggtttggt ttgccctttt cctttatttc
      121 aatatatgcc gtgcacttgt ttgtcgggtc atcttttcat gctttttttt gtcttggttg
      181 tgatgatgtg gtctggttgg gcggtcgttc tagatcggag tagaattaat tctgtttcaa
      241 actacctggt ggatttatta attttggatc tgtatgtgtg tgccatacat attcatagtt
      301 acgaattgaa gatgatggat ggaaatatcg atctaggata ggtatacatg ttgatgcggg
      361 ttttactgat gcatatacag agatgctttt tgttcgcttg gttgtgatga tgtggtgtgg
      421 ttgggcggtc gttcattcgt tctagatcgg agtagaatac tgtttcaaac tacctggtgt
      481 atttattaat tttggaactg tatgtgtgtg tcatacatct tcatagttac gagtttaaga
      541 tggatggaaa tatcgatcta ggataggtat acatgttgat gtgggtttta ctgatgcata
      601 tacatgatgg catatgcagc atctattcat atgctctaac cttgagtacc tatctattat
      661 aataaacaag tatgttttat aattattttg atcttgatat acttggatga tggcatatgc
      721 agcagctata tgtggatttt tttagccctg ccttcatacg ctatttattt gcttggtact
      781 gtttcttttg tcgatgctca ccctgttgtt tggtgttact tctgcaaagc ttatgccaaa
      841 aaagaagaga aaggtttcag gcggctccga caagaagtac tcgatcggcc tcgccattgg
      901 gactaactct gttggctggg ccgtgatcac cgacgagtac aaggtgccct caaagaagtt
      961 caaggtcctg ggcaacaccg atcggcattc catcaagaag aatctcattg gcgctctcct
     1021 gttcgacagc ggcgagacgg ctgaggctac gcggctcaag cgcaccgccc gcaggcggta
     1081 cacgcgcagg aagaatcgca tctgctacct gcaggagatt ttctccaacg agatggcgaa
     1141 ggttgacgat tctttcttcc acaggctgga ggagtcattc ctcgtggagg aggataagaa
     1201 gcacgagcgg catccaatct tcggcaacat tgtcgacgag gttgcctacc acgagaagta
     1261 ccctacgatc taccatctgc ggaagaagct cgtggactcc acagataagg cggacctccg
     1321 cctgatctac ctcgctctgg cccacatgat taagttcagg ggccatttcc tgatcgaggg
     1381 ggatctcaac ccggacaata gcgatgttga caagctgttc atccagctcg tgcagacgta
     1441 caaccagctc ttcgaggaga accccattaa tgcgtcaggc gtcgacgcga aggctatcct
     1501 gtccgctagg ctctcgaagt ctcggcgcct cgagaacctg atcgcccagc tgccgggcga
     1561 gaagaagaac ggcctgttcg ggaatctcat tgcgctcagc ctggggctca cgcccaactt
     1621 caagtcgaat ttcgatctcg ctgaggacgc caagctgcag ctctccaagg acacatacga
     1681 cgatgacctg gataacctcc tggcccagat cggcgatcag tacgcggacc tgttcctcgc
     1741 tgccaagaat ctgtcggacg ccatcctcct gtctgatatt ctcagggtga acaccgagat
     1801 tacgaaggct ccgctctcag cctccatgat caagcgctac gacgagcacc atcaggatct
     1861 gaccctcctg aaggcgctgg tcaggcagca gctccccgag aagtacaagg agatcttctt
     1921 cgatcagtcg aagaacggct acgctgggta cattgacggc ggggcctctc aggaggagtt
     1981 ctacaagttc atcaagccga ttctggagaa gatggacggc acggaggagc tgctggtgaa
     2041 gctcaatcgc gaggacctcc tgaggaagca gcggacattc gataacggca gcatcccaca
     2101 ccagattcat ctcggggagc tgcacgctat cctgaggagg caggaggact tctacccttt
     2161 cctcaaggat aaccgcgaga agatcgagaa gattctgact ttcaggatcc cgtactacgt
     2221 cggcccactc gctaggggca actcccgctt cgcttggatg acccgcaagt cagaggagac
     2281 gatcacgccg tggaacttcg aggaggtggt cgacaagggc gctagcgctc agtcgttcat
     2341 cgagaggatg acgaatttcg acaagaacct gccaaatgag aaggtgctcc ctaagcactc
     2401 gctcctgtac gagtacttca cagtctacaa cgagctgact aaggtgaagt atgtgaccga
     2461 gggcatgagg aagccggctt tcctgtctgg ggagcagaag aaggccatcg tggacctcct
     2521 gttcaagacc aaccggaagg tcacggttaa gcagctcaag gaggactact tcaagaagat
     2581 tgagtgcttc gattcggtcg agatctctgg cgttgaggac cgcttcaacg cctccctggg
     2641 gacctaccac gatctcctga agatcattaa ggataaggac ttcctggaca acgaggagaa
     2701 tgaggatatc ctcgaggaca ttgtgctgac actcactctg ttcgaggacc gggagatgat
     2761 cgaggagcgc ctgaagactt acgcccatct cttcgatgac aaggtcatga agcagctcaa
     2821 gaggaggagg tacaccggct gggggaggct gagcaggaag ctcatcaacg gcattcggga
     2881 caagcagtcc gggaagacga tcctcgactt cctgaagagc gatggcttcg cgaaccgcaa
     2941 tttcatgcag ctgattcacg atgacagcct cacattcaag gaggatatcc agaaggctca
     3001 ggtgagcggc cagggggact cgctgcacga gcatatcgcg aacctcgctg gctcgccagc
     3061 tatcaagaag gggattctgc agaccgtgaa ggttgtggac gagctggtga aggtcatggg
     3121 caggcacaag cctgagaaca tcgtcattga gatggcccgg gagaatcaga ccacgcagaa
     3181 gggccagaag aactcacgcg agaggatgaa gaggatcgag gagggcatta aggagctggg
     3241 gtcccagatc ctcaaggagc acccggtgga gaacacgcag ctgcagaatg agaagctcta
     3301 cctgtactac ctccagaatg gccgcgatat gtatgtggac caggagctgg atattaacag
     3361 gctcagcgat tacgacgtcg atcatatcgt tccacagtca ttcctgaagg atgactccat
     3421 tgacaacaag gtcctcacca ggtcggacaa gaaccggggc aagtctgata atgttccttc
     3481 agaggaggtc gttaagaaga tgaagaacta ctggcgccag ctcctgaatg ccaagctgat
     3541 cacgcagcgg aagttcgata acctcacaaa ggctgagagg ggcgggctct ctgagctgga
     3601 caaggcgggc ttcatcaaga ggcagctggt cgagacacgg cagatcacta agcacgttgc
     3661 gcagattctc gactcacgga tgaacactaa gtacgatgag aatgacaagc tgatccgcga
     3721 ggtgaaggtc atcaccctga agtcaaagct cgtctccgac ttcaggaagg atttccagtt
     3781 ctacaaggtt cgggagatca acaattacca ccatgcccat gacgcgtacc tgaacgcggt
     3841 ggtcggcaca gctctgatca agaagtaccc aaagctcgag agcgagttcg tgtacgggga
     3901 ctacaaggtt tacgatgtga ggaagatgat cgccaagtcg gagcaggaga ttggcaaggc
     3961 taccgccaag tacttcttct actctaacat tatgaatttc ttcaagacag agatcactct
     4021 ggccaatggc gagatccgga agcgccccct catcgagacg aacggcgaga cgggggagat
     4081 cgtgtgggac aagggcaggg atttcgcgac cgtcaggaag gttctctcca tgccacaagt
     4141 gaatatcgtc aagaagacag aggtccagac tggcgggttc tctaaggagt caattctgcc
     4201 taagcggaac agcgacaagc tcatcgcccg caagaaggac tgggatccga agaagtacgg
     4261 cgggttcgac agccccactg tggcctactc ggtcctggtt gtggcgaagg ttgagaaggg
     4321 caagtccaag aagctcaaga gcgtgaagga gctgctgggg atcacgatta tggagcgctc
     4381 cagcttcgag aagaacccga tcgatttcct ggaggcgaag ggctacaagg aggtgaagaa
     4441 ggacctgatc attaagctcc ccaagtactc actcttcgag ctggagaacg gcaggaagcg
     4501 gatgctggct tccgctggcg agctgcagaa ggggaacgag ctggctctgc cgtccaagta
     4561 tgtgaacttc ctctacctgg cctcccacta cgagaagctc aagggcagcc ccgaggacaa
     4621 cgagcagaag cagctgttcg tcgagcagca caagcattac ctcgacgaga tcattgagca
     4681 gatttccgag ttctccaagc gcgtgatcct ggccgacgcg aatctggata aggtcctctc
     4741 cgcgtacaac aagcaccgcg acaagccaat cagggagcag gctgagaata tcattcatct
     4801 cttcaccctg acgaacctcg gcgcccctgc tgctttcaag tacttcgaca caactatcga
     4861 tcgcaagagg tacacaagca ctaaggaggt cctggacgcg accctcatcc accagtcgat
     4921 taccggcctc tacgagacgc gcatcgacct gtctcagctc gggggcgacg aattctccgg
     4981 cggatcttct ggaggatcta gcggctccga gacaccagga acatccgaat ccgctacacc
     5041 agagtcttct ggaggatcta gcggaggatc ttccgaggtg gagttctctc acgagtattg
     5101 gatgaggcac gctcttacac ttgctaagag agcttgggac gaaagagaag tgccagttgg
     5161 cgccgttctt gtgcataata atagggtgat cggcgagggt tggaatagac caattggaag
     5221 gcatgatcca acagctcacg cagagattat ggctctcaga caaggcggcc tcgttatgca
     5281 gaactacagg ctcattgacg ctacactcta cgtgacactc gaaccttgcg ttatgtgcgc
     5341 cggagctatg attcattcta ggattggcag ggtcgtgttt ggagctaggg acgctaaaac
     5401 aggagccgcc ggatctctta tggacgtgtt gcatcatcca ggcatgaacc atagggtgga
     5461 gattacagag ggcattcttg cagacgagtg cgctgctctt ctttccgatt tcttcaggat
     5521 gagaaggcag gagattaagg cccagaagaa ggctcagtct tctacagata gcggaggatc
     5581 ttccggagga tctagcggct ccgagacacc aggaacatcc gaaagcgcta caccagaatc
     5641 tagcggaggc tcttccggag gatcttctga agtggagttc tcccacgagt attggatgag
     5701 gcacgctctt acacttgcta aaagggctag ggacgaaagg gaagttccag ttggagctgt
     5761 tctcgtgctc aataacaggg tgattggcga gggttggaat agagccattg gactccatga
     5821 tccaacagct cacgcagaga ttatggctct tagacaaggc ggcctcgtta tgcagaatta
     5881 cagactcatc gacgccacac tctacgttac cttcgaacct tgcgttatgt gcgccggagc
     5941 tatgatccat tctaggattg gcagggtcgt gttcggcgtt agaaacgcta agacaggagc
     6001 tgcaggctct cttatggacg ttcttcatta cccaggcatg aatcatagag tggagatcac
     6061 agaaggcatt cttgcagacg agtgcgcagc tctcctttgc tatttcttca ggatgccgag
     6121 gcaagttttc aacgctcaga agaaggccca gtcttctaca gataagagac cagcagctac
     6181 aaagaaggcc ggacaagcta agaagaagaa gtaggaattc ggtacgctga aatcaccagt
     6241 ctctctctac aaatctatct ctctctattt tctccataaa taatgtgtga gtagtttccc
     6301 gataagggaa attagggttc ttatagggtt tcgctcatgt gttgagcata taagaaaccc
     6361 ttagtatgta tttgtatttg taaaatactt ctatcaataa aatttctaat tcctaaaacc
     6421 aaaatccagt actaaaatcc agatctccta aagtccctat agatctttgt cgtgaatata
     6481 aaccagacac gagacgacta aacctggagc ccagacgccg ttcgaagcta gaagtaccgc
     6541 ttaggcagga ggccgttagg gaaaagatgc taaggcaggg ttggttacgt tgactccccc
     6601 gtaggtttgg tttaaatatg atgaagtgga cggaaggaag gaggaagaca aggaaggata
     6661 aggttgcagg ccctgtgcaa ggtaagaaga tggaaatttg atagaggtac gctactatac
     6721 ttatactata cgctaaggga atgcttgtat ttatacccta taccccctaa taacccctta
     6781 tcaatttaag aaataatccg cataagcccc cgcttaaaaa ttggtatcag agccatgaat
     6841 aggtctatga ccaaaactca agaggataaa acctcaccaa aatacgaaag agttcttaac
     6901 tctaaagata aaagatcttt caagatcaaa actagttccc tcacaccggt gacggggatc
     6961 gcatgcgata tctcgagatc tagcttggcg taatcatggt catagctgtt tcctgtgtga
     7021 aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc
     7081 tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc
     7141 cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc
     7201 ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt
     7261 cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca
     7321 ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa
     7381 aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat
     7441 cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc
     7501 cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc
     7561 gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt
     7621 tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac
     7681 cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg
     7741 ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca
     7801 gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc
     7861 gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa
     7921 accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa
     7981 ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac
     8041 tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta
     8101 aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt
     8161 taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata
     8221 gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc
     8281 agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac
     8341 cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag
     8401 tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac
     8461 gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc
     8521 agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg
     8581 gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc
     8641 atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct
     8701 gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc
     8761 tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc
     8821 atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc
     8881 agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc
     8941 gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca
     9001 cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt
     9061 tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt
     9121 ccgcgcacat ttccccgaaa agtgccacct gccagtgcca agctaattcg agctcggtac
     9181 ctgacccggt cgtgcccctc tctagagata atgagcattg catgtctaag ttataaaaaa
     9241 ttaccacata ttttttttgt cacacttgtt tgaagtgcag tttatctatc tttatacata
     9301 tatttaaact ttactctacg aataatataa tctatagtac tacaataata tcagtgtttt
     9361 agagaatcat ataaatgaac agttagacat ggtctaaagg acaattgagt attttgacaa
     9421 caggactcta cagttttatc tttttagtgt gcatgtgttc tccttttttt ttgcaaatag
     9481 cttcacctat ataatacttc atccatttta ttagtacatc catttagggt ttagggttaa
     9541 tggtttttat agactaattt ttttagtaca tctattttat tctattttag cctctaaatt
     9601 aagaaaacta aaactctatt ttagtttttt tatttaataa tttagatata aaatagaata
     9661 aaataaagtg actaaaaatt aaacaaatac cctttaagaa attaaaaaaa ctaaggaaac
     9721 atttttcttg tttcgagtag ataatgccag cctgttaaac gccgtcgacg agtctaacgg
     9781 acaccaacca gcgaaccagc agcgtcgcgt cgggccaagc gaagcagacg gcacggcatc
     9841 tctgtcgctg cctctggacc cctctcgatc gagagttccg ctccaccgtt ggacttgctc
     9901 cgctgtcggc atccagaaat tgcgtggcgg agcggcagac gtgagccggc acggcaggcg
     9961 gcctcctcct cctctcacgg caccggcagc tacgggggat tcctttccca ccgctccttc
    10021 gctttccctt cctcgcccgc cgtaataaat agacaccccc tccacaccct ctttccccaa
    10081 cctcgtgttg ttcggagcgc acacacacac aaccagatct cccccaaatc cacccgtcgg
    10141 cacctccgct tcaaggtacg ccgctcgtcc tccncccccc cccctctcta ccttctctag
    10201 atcggcgttc cggtccatgg ttagggcccg gtagttctac ttctgttcat gtttgtgtta
    10261 gatccgtgtt tgtgttagat ccgtgctgct agcgttcgta cacggatgcg acctgtacgt
    10321 cagacacgtt ctgattgcta ac
//