| 出品公司: | Clontech |
|---|---|
| 载体名称: | pEGFP |
| 质粒类型: | 细菌表达载体 |
| 高拷贝/低拷贝: | -- |
| 克隆方法: | -- |
| 启动子: | lac |
| 载体大小: | 3355 bp |
| 5' 测序引物及序列: | EGFP-N[CGTCCGTCCAGCGACCAG] |
| 3' 测序引物及序列: | -- |
| 载体标签: | EGFP |
| 载体抗性: | 氨苄青霉素 |
| 筛选标记: | -- |
| 克隆菌株: | -- |
| 宿主细胞（系）: | -- |
| 备注: | EGFP标签。对于带有荧光标记的其他细菌表达载体 |
| 产品目录号: | -- |
| 稳定性: | -- |
| 组成型/诱导型: | -- |
| 病毒/非病毒: | -- |
买家导航
pEGFP载体质粒基本信息
pEGFP质粒图谱载体图谱和pEGFP载体序列质粒序列多克隆位点信息
pEGFP质粒载体简介
pEGFP质粒序列载体序列
LOCUS pEGFP 3355 bp ds-DNA circular SYN 19-OCT-2017
DEFINITION Vector for expressing EGFP in bacteria.
ACCESSION .
VERSION .
KEYWORDS pEGFP
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 3355)
AUTHORS Clontech (TaKaRa)
TITLE Direct Submission
JOURNAL Exported Friday, February 21, 2020 from SnapGene Viewer 3.1.4
http://www.snapgene.com
COMMENT Translation from the lacZ start codon produces a fusion protein in
E. coli.
FEATURES Location/Qualifiers
source 1..3355
/organism="synthetic DNA construct"
/lab_host="Escherichia coli"
/mol_type="other DNA"
promoter 143..173
/note="lac promoter"
/note="promoter for the E. coli lac operon"
protein_bind 181..197
/bound_moiety="lac repressor encoded by lacI"
/note="lac operator"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
CDS 217..219
/codon_start=1
/product="lacZ start codon"
/note="ATG"
/translation="M"
CDS 289..1008
/codon_start=1
/product="enhanced GFP"
/note="EGFP"
/note="mammalian codon-optimized"
/translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
EFVTAAGITLGMDELYK"
promoter 1450..1554
/gene="bla"
/note="AmpR promoter"
CDS 1555..2415
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/note="AmpR"
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 2586..3174
/direction=RIGHT
/note="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc
61 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc
121 tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa
181 ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac gccaagcttg
241 catgcctgca ggtcgactct agaggatccc cgggtaccgg tcgccaccat ggtgagcaag
301 ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg agctggacgg cgacgtaaac
361 ggccacaagt tcagcgtgtc cggcgagggc gagggcgatg ccacctacgg caagctgacc
421 ctgaagttca tctgcaccac cggcaagctg cccgtgccct ggcccaccct cgtgaccacc
481 ctgacctacg gcgtgcagtg cttcagccgc taccccgacc acatgaagca gcacgacttc
541 ttcaagtccg ccatgcccga aggctacgtc caggagcgca ccatcttctt caaggacgac
601 ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc
661 gagctgaagg gcatcgactt caaggaggac ggcaacatcc tggggcacaa gctggagtac
721 aactacaaca gccacaacgt ctatatcatg gccgacaagc agaagaacgg catcaaggtg
781 aacttcaaga tccgccacaa catcgaggac ggcagcgtgc agctcgccga ccactaccag
841 cagaacaccc ccatcggcga cggccccgtg ctgctgcccg acaaccacta cctgagcacc
901 cagtccgccc tgagcaaaga ccccaacgag aagcgcgatc acatggtcct gctggagttc
961 gtgaccgccg ccgggatcac tctcggcatg gacgagctgt acaagtaaag cggccgcgac
1021 tctagaattc caactgagcg ccggtcgcta ccattaccaa cttgtctggt gtcaaaaata
1081 ataggcctac tagtcggccg tacgggccct ttcgtctcgc gcgtttcggt gatgacggtg
1141 aaaacctctg acacatgcag ctcccggaga cggtcacagc ttgtctgtaa gcggatgccg
1201 ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg cgggtgtcgg ggctggctta
1261 actatgcggc atcagagcag attgtactga gagtgcacca tatgcggtgt gaaataccgc
1321 acagatgcgt aaggagaaaa taccgcatca ggcggcctta agggcctcgt gatacgccta
1381 tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg
1441 ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg
1501 ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt
1561 attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt
1621 gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg
1681 ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa
1741 cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt
1801 gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag
1861 tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt
1921 gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga
1981 ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt
2041 tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta
2101 gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg
2161 caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc
2221 cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt
2281 atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg
2341 gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg
2401 attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa
2461 cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa
2521 atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga
2581 tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg
2641 ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact
2701 ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac
2761 cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg
2821 gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg
2881 gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga
2941 acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc
3001 gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg
3061 agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc
3121 tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc
3181 agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt
3241 cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc
3301 gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaag
//
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