| 质粒名称: | pAB675 |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 176578 |
| 存储实验室: | Arpita Bose |
|
载体骨架基本信息 |
|
| 载体名称: | pRhokS_2 |
| 载体出品公司: | -- |
| 空载体大小: | -- |
| 完整质粒大小: | -- |
| 载体修饰: | -- |
| 载体类型: | 细菌表达 |
| 筛选标记: | -- |
| 载体抗性: | 卡那霉素50 μg/mL |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5a |
| 拷贝数: | 高拷贝 |
|
插入基因信息 |
|
| 插入基因名称: | phaJ, ter, adhE2 |
| 物种来源: | -- |
| 插入大小: | -- |
| 基因突变: | -- |
| GenBank编号 | -- |
| 启动子: | -- |
| 标签/融合蛋白: | -- |
| 克隆方法: | 限制性内切酶 |
| 5’克隆位点: | -- |
| 3’克隆位点: | -- |
| 5’测序引物 | -- |
| 3’测序引物 | -- |
|
文献引用方法 |
|
| 材料和方法部分: | pAB675 was a gift from Arpita Bose (Addgene plasmid # 176578 ; http://n2t.net/addgene:176578 ; RRID:Addgene_176578) |
买家导航
pAB675载体质粒基本信息
pAB675质粒图谱载体图谱和pAB675载体序列质粒序列多克隆位点信息
pAB675质粒载体简介
pAB675质粒序列载体序列
LOCUS pAB675 10844 bp ds-DNA circular SYN 16-NOV-2021
DEFINITION Expressing three of the five genes of the n-butanol pathway..
ACCESSION .
VERSION .
KEYWORDS pAB675
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 10844)
TITLE n-butanol pathway plasmids
JOURNAL Unpublished
REFERENCE 2 (bases 1 to 10844)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Nov 16, 2021 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..10844
/organism="synthetic DNA construct"
/mol_type="other DNA"
primer_bind 793..812
/label=CAT-R
/note="Chloramphenicol resistance gene, reverse primer"
promoter complement(879..981)
/label=cat promoter
/note="promoter of the E. coli cat gene encoding
chloramphenicol acetyltransferase"
primer_bind complement(1098..1116)
/label=pBRforEco
/note="pBR322 vectors, upsteam of EcoRI site, forward
primer"
rep_origin 2374..3145
/label=pBBR1 oriV
/note="replication origin of the broad-host-range plasmid
pBBR1 from Bordetella bronchiseptica; requires the pBBR1
Rep protein for replication"
CDS 3146..3808
/codon_start=1
/product="replication protein for the broad-host-range
plasmid pBBR1 from Bordetella bronchiseptica"
/label=pBBR1 Rep
/translation="MATQSREIGIQAKNKPGHWVQTERKAHEAWAGLIARKPTAAMLLH
HLVAQMGHQNAVVVSQKTLSKLIGRSLRTVQYAVKDLVAERWISVVKLNGPGTVSAYVV
NDRVAWGQPRDQLRLSVFSAAVVVDHDDQDESLLGHGDLRRIPTLYPGEQQLPTGPGEE
PPSQPGIPGMEPDLPALTETEEWERRGQQRLPMPDEPCFLDDGEPLEPPTRVTLPRR"
primer_bind 4539..4558
/label=F1ori-R
/note="F1 origin, reverse primer"
terminator 4682..4729
/label=T7 terminator
/note="transcription terminator for bacteriophage T7 RNA
polymerase"
primer_bind 4725..4743
/label=T7 Term
/note="T7 terminator, reverse primer"
CDS complement(4796..4819)
/codon_start=1
/product="peptide that binds Strep-Tactin(R), an engineered
form of streptavidin"
/label=Strep-Tag II
/translation="WSHPQFEK"
terminator 4827..4866
/label=fd terminator
/note="central terminator from bacteriophage fd (Otsuka and
Kunisawa, 1982)"
RBS 9116..9138
/note="efficient ribosome binding site from bacteriophage
T7 gene 10 (Olins and Rangwala, 1989)"
protein_bind 9153..9177
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
primer_bind complement(9177..9196)
/label=T7
/note="T7 promoter, forward primer"
promoter complement(9178..9196)
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
CDS complement(9241..10035)
/codon_start=1
/gene="aph(3')-II (or nptII)"
/product="aminoglycoside phosphotransferase from Tn5"
/label=NeoR/KanR
/note="confers resistance to neomycin, kanamycin, and G418
(Geneticin(R))"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
primer_bind complement(9352..9371)
/label=Neo-F
/note="Neomycin resistance gene, forward primer"
primer_bind 9962..9981
/label=Neo-R
/note="Neomycin resistance gene, reverse primer"
ORIGIN
1 cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg tttcccgact
61 ggaaagcggg cagtgagcgc aacgcaatta atgtaagtta gctcactcat taggcaccgg
121 gatctcgacc gatgcccttg agagccttca acccagtcag ctccttccgg tgggcgcggg
181 gcatgactat aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgcatg
241 cataaaaact gttgtaattc attaagcatt ctgccgacat ggaagccatc acaaacggca
301 tgatgaacct gaatcgccag cggcatcagc accttgtcgc cttgcgtata atatttgccc
361 atggtgaaaa cgggggcgaa gaagttgtcc atattggcca cgtttaaatc aaaactggtg
421 aaactcaccc agggattggc tgagacgaaa aacatattct caataaaccc tttagggaaa
481 taggccaggt tttcaccgta acacgccaca tcttgcgaat atatgtgtag aaactgccgg
541 aaatcgtcgt ggtattcact ccagagcgat gaaaacgttt cagtttgctc atggaaaacg
601 gtgtaacaag ggtgaacact atcccatatc accagctcac cgtctttcat tgccatacgg
661 aattccggat gagcattcat caggcgggca agaatgtgaa taaaggccgg ataaaacttg
721 tgcttatttt tctttacggt ctttaaaaag gccgtaatat ccagctgaac ggtctggtta
781 taggtacatt gagcaactga ctgaaatgcc tcaaaatgtt ctttacgatg ccattgggat
841 atatcaacgg tggtatatcc agtgattttt ttctccattt tagcttcctt agctcctgaa
901 aatctcgata actcaaaaaa tacgcccggt agtgatctta tttcattatg gtgaaagttg
961 gaacctctta cgtgccgatc aacgtctcat tttcgccaaa agttggccca gggcttcccg
1021 gtatcaacag ggacaccagg atttatttat tctgcgaagt gatcttccgt cacaggtatt
1081 tattcgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat
1141 aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcgccc gcgttcctgc
1201 tggcgctggg cctgtttctg gcgctggact tcccgctgtt ccgtcagcag cttttcgccc
1261 acggccttga tgatcgcggc ggccttggcc tgcatatccc gattcaacgg ccccagggcg
1321 tccagaacgg gcttcaggcg ctcccgaagg tctcgggccg tctcttgggc ttgatcggcc
1381 ttcttgcgca tctcacgcgc tcctgcggcg gcctgtaggg caggctcata cccctgccga
1441 accgcttttg tcagccggtc ggccacggct tccggcgtct caacgcgctt tgagattccc
1501 agcttttcgg ccaatccctg cggtgcatag gcgcgtggct cgaccgcttg cgggctgatg
1561 gtgacgtggc ccactggtgg ccgctccagg gcctcgtaga acgcctgaat gcgcgtgtga
1621 cgtgccttgc tgccctcgat gccccgttgc agccctagat cggccacagc ggccgcaaac
1681 gtggtctggt cgcgggtcat ctgcgctttg ttgccgatga actccttggc cgacagcctg
1741 ccgtcctgcg tcagcggcac cacgaacgcg gtcatgtgcg ggctggtttc gtcacggtgg
1801 atgctggccg tcacgatgcg atccgccccg tacttgtccg ccagccactt gtgcgccttc
1861 tcgaagaacg ccgcctgctg ttcttggctg gccgacttcc accattccgg gctggccgtc
1921 atgacgtact cgaccgccaa cacagcgtcc ttgcgccgct tctctggcag caactcgcgc
1981 agtcggccca tcgcttcatc ggtgctgctg gccgcccagt gctcgttctc tggcgtcctg
2041 ctggcgtcag cgttgggcgt ctcgcgctcg cggtaggcgt gcttgagact ggccgccacg
2101 ttgcccattt tcgccagctt cttgcatcgc atgatcgcgt atgccgccat gcctgcccct
2161 cccttttggt gtccaaccgg ctcgacgggg gcagcgcaag gcggtgcctc cggcgggcca
2221 ctcaatgctt gagtatactc actagacttt gcttcgcaaa gtcgtgaccg cctacggcgg
2281 ctgcggcgcc ctacgggctt gctctccggg cttcgccctg cgcggtcgct gcgctccctt
2341 gccagcccgt ggatatgtgg acgatggccg cgagcggcca ccggctggct cgcttcgctc
2401 ggcccgtgga caaccctgct ggacaagctg atggacaggc tgcgcctgcc cacgagcttg
2461 accacaggga ttgcccaccg gctacccagc cttcgaccac atacccaccg gctccaactg
2521 cgcggcctgc ggccttgccc catcaatttt tttaattttc tctggggaaa agcctccggc
2581 ctgcggcctg cgcgcttcgc ttgccggttg gacaccaagt ggaaggcggg tcaaggctcg
2641 cgcagcgacc gcgcagcggc ttggccttga cgcgcctgga acgacccaag cctatgcgag
2701 tgggggcagt cgaagggcga agcccgcccg cctgcccccc gagcctcacg gcggcgagtg
2761 cgggggttcc aagggggcag cgccaccttg ggcaaggccg aaggccgcgc agtcgatcaa
2821 caagccccgg aggggccact ttttgccgga gggggagccg cgccgaaggc gtgggggaac
2881 cccgcagggg tgcccttctt tgggcaccaa agaactagat atagggcgaa atgcgaaaga
2941 cttaaaaatc aacaacttaa aaaagggggg tacgcaacag ctcattgcgg caccccccgc
3001 aatagctcat tgcgtaggtt aaagaaaatc tgtaattgac tgccactttt acgcaacgca
3061 taattgttgt cgcgctgccg aaaagttgca gctgattgcg catggtgccg caaccgtgcg
3121 gcacccctac cgcatggaga taagcatggc cacgcagtcc agagaaatcg gcattcaagc
3181 caagaacaag cccggtcact gggtgcaaac ggaacgcaaa gcgcatgagg cgtgggccgg
3241 gcttattgcg aggaaaccca cggcggcaat gctgctgcat cacctcgtgg cgcagatggg
3301 ccaccagaac gccgtggtgg tcagccagaa gacactttcc aagctcatcg gacgttcttt
3361 gcggacggtc caatacgcag tcaaggactt ggtggccgag cgctggatct ccgtcgtgaa
3421 gctcaacggc cccggcaccg tgtcggccta cgtggtcaat gaccgcgtgg cgtggggcca
3481 gccccgcgac cagttgcgcc tgtcggtgtt cagtgccgcc gtggtggttg atcacgacga
3541 ccaggacgaa tcgctgttgg ggcatggcga cctgcgccgc atcccgaccc tgtatccggg
3601 cgagcagcaa ctaccgaccg gccccggcga ggagccgccc agccagcccg gcattccggg
3661 catggaacca gacctgccag ccttgaccga aacggaggaa tgggaacggc gcgggcagca
3721 gcgcctgccg atgcccgatg agccgtgttt tctggacgat ggcgagccgt tggagccgcc
3781 gacacgggtc acgctgccgc gccggtagca cttgggttgc gcagcaaccc gtaagtgcgc
3841 tgttccagac tatcggctgt agccgcctcg ccgccctata ccttgtctgc ctccccgcgt
3901 tgcgtcgcgg tgcatggagc cgggccacct cgacctgaat ggaagccggc ggcacctcgc
3961 taacggattc accgttttta tcaggctctg ggaggcagaa taaatgatca tatcgtcaat
4021 tattacctcc acggggagag cctgagcaaa ctggcctcag gcatttgaga agcacacggt
4081 cacactgctt ccggtagtca ataaaccggt aaaccagcaa tagacataag cggctattta
4141 acgaccctgc cctgaaccga cgaccgggtc gaatttgctt tcgaatttct gccattcatc
4201 cgcttattat cacttattca ggcgtagcaa ccaggcgttt aagggcacca ataactgcct
4261 taaaaaaatt acgccccgcc ctgccactca tcgcagtacg gcctattggt taaaaaatga
4321 gctgatttaa caaaaattta acgcgaattt taacaaaata ttaacgctta caatttccat
4381 tcgccattca ggctgcgcaa ctgttgggaa gggcgtgaac catcacccta atcaagtttt
4441 ttggggtcga ggtgccgtaa agcactaaat cggaacccta aagggagccc ccgatttaga
4501 gcttgacggg gaaagccggc gaacgtggcg agaaaggaag ggaagaaagc gaaaggagcg
4561 ggcgctaggg cgctggcaag tgtagcggtc acgctgcgcg taaccaccac acccgccgcg
4621 cttaatgcgc cgctacaggg cgcgtcccat tcgccaatcc ggatatagtt cctcctttca
4681 gcaaaaaacc cctcaagacc cgtttagagg ccccaagggg ttatgctagt tattgctcag
4741 cggtggcagc agccaactca gcttcctttc gggctttgtt agcagccgga tctcactttt
4801 cgaactgcgg atgcgaccac tgcagctcca aaaaaaaagg ctccaaaagg agcctttaat
4861 tgtatcggtt tagcatgcat ttagaagctt ttgatgtaga tgtccttcag ctccgagatc
4921 agcgggtagc gcgggttcgc cgtggtgcac tggtcatcga acgccagctc gctcatcttg
4981 tcgagggtgt tgtagaagtc cttcttattg atgcccgcgg cggagatatt ctgcgggatc
5041 gacagatcga tcttgagctt gctgatcgct tcgatcagcg cggtcacctt ttcggtgtcc
5101 gaggtgccct tcagattcag gtattcggcg atttccgcgt acttgcgctt cgcattcggc
5161 gacttgtact gcgggaacgc ggtctgcttc gtcgggcagt cggtcgcgtt atacttgatg
5221 acctcctcga tgagcaccgc gcaggcgatg ccgtgcggca cgtggtgcat ggcgccgagc
5281 ttatgcgcca tgctgtggca gacgcccagg aaggcgttgg cgaaggccat gccggcgatg
5341 ttgctggcgt gggccatctt ttcgcgcgct tcgatgtcgt tggtgccgtt tttgtacgcg
5401 cgcggcaggt atttgaagat catcttgatc gcgcgcagcg cgagttcgtc ggtatagtcg
5461 gtggccatca ccgacacata cgcctcgatc gcgtggacca gcgcgtcgat gccggtggcg
5521 gcggtgagct tccgcggcat gttcagcatc agctcggtgt cgatgatcgc catattcggg
5581 gtcagctcat agctggtcag cgggtacttc atccccgtct cgtcgttggt gatgaccgcg
5641 aacggggtgg cctccgaccc cgtgccggcg gtggtcggga tcgcgacgga gatcgccttg
5701 gtgcccagct tcgggaaatt gcagatgcgc ttgcggatgt ccatgaagtt gatggccaga
5761 ttctcgatct ccgcctccgg atactcgtac agcaggtgca tcaccttggc cgcatccatc
5821 gggctgccgc cgccgatcga gatgatcgtg tccggctcga agttcagcat ctccttcgcg
5881 cctttcttca ccgaatcgat ggtcgggtcg gatttgatgt cggtgaagat gctgtacttg
5941 atgtcgattt cgtcgaggac cttggtgatc ttgttgacgt agcccagctt gaacaggtcc
6001 ttgtcggtca cgatgaaggc ccgtttcttg ttcatgtcct tgagctcctt cagggcgaag
6061 cggaggcagc cgtacttgaa gtagatcttc tgcggcacct tgaaccacag catgttttcc
6121 cggcgttcgg cgacggattt gatgttcagc aggtgttttg gttccacgtt ctgcgagacg
6181 ctattgccgc cccaggtgcc gcacccgagc gtgaagctcg gcgcgatggc gaaattgtag
6241 aggtcgcccg aggcgccctg cgagcttggc atgttgatga aggtgcggga ggtcttcatg
6301 gcgaggccga actctttcac tttatcctta ttgttctggc tgtcgatata cagcgacgag
6361 gtatggcccg agccgcccag ctcgatcagg cgctgggcct tcttcagggc ttcgtcgaag
6421 tctttgacct tatacatcgc cagcaccggg ctcagcttct cgtggctgaa cagctccgac
6481 ttctcgacgg actgcacctc gccgatgagg atcttggtgg tctgcggcac ctcgatgccg
6541 gccatcttgg cgatgatata ggccgacttg cccacgatgt ccgcattgat cgcgccgttc
6601 ttaaacatgg tctccttgat cttcgcgatc tcattctggt tcaggatata cgagccgcgc
6661 ttgacaaact cctccttgac tttctcgtag atgctgttca tgaccaggat gctctgctcg
6721 ctggcgcaga tcaccccgtt gtcgtaggtc ttggacagga tgatgctcga caccgccatg
6781 tcgatgtcgg cgctctcgtc gatgatggcc ggggtgttcc ccgcgcccac gccgatcgcg
6841 ggcttgccgg acgagtacgc cgctttgacc atcgaggggc cgccggtggc gaggatgatg
6901 tcggcctccg acatgagatc ctggctcagc tcgatcgacg gttcatcgat ccagccgatg
6961 atgttcttcg gcgcgccggc cttcacggcg gcgtccagga tgagcttggc ggccgcgatg
7021 gtggacttct tggcgcgcgg atgcgggctg aagaagatcg cgttgcgggt cttcagcgag
7081 atcaggctct tgaagatggc cgtggaggtc gggttggtgg tggggacgat cgccgcgacg
7141 atcccgatgg gttccgccac cttggtgatg ccgaggctgt cgtcgtggtc gatgatgccg
7201 caggtcttct cgttcttgta cttattgtag atgtactccg cggcgaagtg gttcttgatg
7261 atcttatcct ccaccaggcc gatgccggtc tcctcgacgg ccagcttggc gaggttgatg
7321 cgctccttgg cggcggcgat ggcgcactgc ttgaagattt tgtcgacctg ctcctgcgtg
7381 taggtggcga acttcttctg ggcctcccgc agctcattca gcttctgctt cagctccttt
7441 tggttggtga ccttcatgcg aaacgatcct catcctgaat tcttagatgc ggtcgaagcg
7501 ctcgacctcc gcctcgtagt tgatgccctc gacatcgaag ccgttgctgg cgaggaagtc
7561 gtggcggtag ccggccaggt cggtcaggct ctcggcgttc tcgccggtga ccttctccat
7621 cagcgcgctg accgcctttt ggacgtcctc ctcgagctcc caatcgtcga tccggatccg
7681 attttcctcg tccaccggga tggtcccgtc cttgcggtac aggcgctcgg cgtacaggcg
7741 cgtgatctgc tcgatgcagc cttcatggtt gcccttctct ttcatcacct taaacaggga
7801 cgcgaggtac agcgggatga ccgggatcac ggcgctcgcc cgggtgacca ggcccttgtt
7861 gaccgagacg aacgcgcgga tggaggggtt ctccttattg aggcggtgcg cggtggcctc
7921 gaggtgctcc ttggccttgc cgatggtgcc cttccggtag agggcctggg tcgcttccgg
7981 gccgatatac gagtaggcga gggtgatgca gccctcctcc agcaggccct ccttgctcag
8041 ctgcttgatc cagcgttccc agtcctcgcc gcccatcacc ttgacggtgg ccgccgcctc
8101 ctcatcgttg gccggttccg cgctgatctc cttgagctcc ccggtgaacg ggtcgacggt
8161 cttgcccgtg aaggtcttgc cgaacggctt caggacgctt ttgtgcatga tcccggtatc
8221 cgggtccgtg cgcaccgggc tcgccagcga gtagacgatc aggtcaaact tgatgccctt
8281 ctttttggcc tcctcgatga cctgcgcctt gatctcatcc gagaacgcgt cgccatcgat
8341 ggtcacgctg tacaggccct cgcgcttggc cgcctcgtca aaggccagat tgttgtacca
8401 gcccggggtg ccgtacttcg tctcgctgcc ggccttctcg aacgagacgc cgatcgtggc
8461 ggcgccgtag ccgaaggcgg cggtgatgcg cgacgccagg ccatagccgt tcgagcagcc
8521 gaggacgagg acgttcttcg gggccttcgc gcccgccttg acttccgcgg tgatgcgctt
8581 cttggtgtac tcgatctggt cctcgacgcc ctttttgcag ccctgcgggt gggcgttcag
8641 gcagatattg ttgcgcacca tcggcttgac gatcatgcga aacgatcctc atcctgcatg
8701 catttacggc agcttcacca ccgcttcccc ggtcacggcc agcgcgccgc cctgcgtaaa
8761 gatgcgggtg gtgagggtgg cgattggctt gtcctcgcgc agcgcggtga cttccacctc
8821 ggccgtgact tcgtcgccga cgaacaccgg cagcttaaag gacaggctct ggcccaggta
8881 gatggagcct ttccccggca gctgttggcc gaggaggccg ctgaacaggc tcgcgaggag
8941 catgccatgc acgatcgggc gttcgaacgc cgtggtcgcc gcaaacgccg ggtccagatg
9001 cagcgggttg aagtcctcgg agagggccgc gaacgcggcg acttccgccg cgccaaaccg
9061 cttgctcagg cgcgctttct ggcccacctc cagggactgc gcggacatat gtatatctcc
9121 ttcttaaagt taaacaaaat tatttctaga ggggaattgt tatccgctca caattcccct
9181 atagtgagtc gtattaattt cgcgggatcg agatcaattc cgcgaacccc agagtcccgc
9241 tcagaagaac tcgtcaagaa ggcgatagaa ggcgatgcgc tgcgaatcgg gagcggcgat
9301 accgtaaagc acgaggaagc ggtcagccca ttcgccgcca agctcttcag caatatcacg
9361 ggtagccaac gctatgtcct gatagcggtc cgccacaccc agccggccac agtcgatgaa
9421 tccagaaaag cggccatttt ccaccatgat attcggcaag caggcatcgc catgggtcac
9481 gacgagatcc tcgccgtcgg gcatccgcgc cttgagcctg gcgaacagtt cggctggcgc
9541 gagcccctga tgctcttcgt ccagatcatc ctgatcgaca agaccggctt ccatccgagt
9601 acgtgctcgc tcgatgcgat gtttcgcttg gtggtcgaat gggcaggtag ccggatcaag
9661 cgtatgcagc cgccgcattg catcagccat gatggatact ttctcggcag gagcaaggtg
9721 agatgacagg agatcctgcc ccggcacttc gcccaatagc agccagtccc ttcccgcttc
9781 agtgacaacg tcgagcacag ctgcgcaagg aacgcccgtc gtggccagcc acgatagccg
9841 cgctgcctcg tcttggagtt cattcagggc accggacagg tcggtcttga caaaaagaac
9901 cgggcgcccc tgcgctgaca gccggaacac ggcggcatca gagcagccga ttgtctgttg
9961 tgcccagtca tagccgaata gcctctccac ccaagcggcc ggagaacctg cgtgcaatcc
10021 atcttgttca atcatgcgaa acgatcctca tcctgtctct tgatcagatc ttgatcccct
10081 gcgccatcag atccttggcg gcaagaaagc catccagttt actttgcagg gcttcccaac
10141 cttaccagag ggcgccccag ctggcaattc cggttcgctt gctgtccata aaaccgccca
10201 gtctagctat cgccatgtaa gcccactgca agctacctgc tttctctttg cgcttgcgtt
10261 ttcccttgtc cagatagccc agtagctgac attcatccgg ggtcagcacc gtttctgcgg
10321 actggctttc tacgtgttcc gcttccttta gcagcccttg cgccctgagt gcttgcggca
10381 gcgtgaagct agcggaattc attaagttct gtctcggcgc gtctgcgtct ggctggctgg
10441 cataaatatc tcactcgcaa tcaaattcag ccgatagcgg aacgggaagg cgactggagt
10501 gccatgtccg gttttcaaca aaccatgcaa atgctgaatg agggcatcgt tcccactgcg
10561 atgctggttg ccaacgatca gatggcgctg ggcgcaatgc gcgccattac cgagtccggg
10621 ctgcgcgttg gtgcggatat ctcggtagtg ggatacgacg ataccgaaga cagctcatgt
10681 tatatcccgc cgttaaccac catcaaacag gattttcgcc tgctggggca aaccagcgtg
10741 gaccgcttgc tgcaactctc tcagggccag gcggtgaagg gcaatcagct gttgcccgtc
10801 tcactggtga aaagaaaaac caccctggcg cccaatacgc aaac
//
pAB675载体图谱质粒图谱pdf版和pAB675载体序列质粒序列等相关资料下载
pAB675质粒载体应用举例
- 上一篇：EC2_10_dCas9_VPR_HC_sgRNA
- 下一篇：pBK086
