pQL163

LOCUS       pQL163             7534 bp DNA     circular SYN 12-JUN-2026
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7534)
  AUTHORS   Westlake University
  TITLE     Direct Submission
  JOURNAL   Exported Sep 29, 2026 from SnapGene 6.0.2
            https://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..7534
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     212..232
                     /label=QL426-F
     primer_bind     216..236
                     /label=QL414-F
     enhancer        238..617
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        619..817
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             835..1015
                     /label=HIV-1 5 LTR
     misc_feature    1062..1187
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1126..1170
                     /label=HIV-1 psi pack
     misc_feature    1222..1227
                     /label=U6 terminator
     misc_feature    1251..1256
                     /label=U6 terminator
     misc_feature    1665..1670
                     /label=U6 terminator
     misc_feature    1684..1917
                     /label=RRE
     misc_feature    2444..2561
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2620..2932
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for mouse U6 snRNA (Das 
                     et al., 1988)"
     misc_feature    2845..2850
                     /label=U6 terminator
     primer_bind     2949..2965
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2994..3008)
                     /label=QL152-R
     misc_recomb     3007..3040
                     /label=LoxP
     protein_bind    3007..3040
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
     primer_bind     3048..3089
                     /label=QL426-F
     primer_bind     3073..3093
                     /label=QL414-F
     misc_feature    3094..3292
                     /label=-230
     misc_feature    3094..3292
                     /label=-1430
     misc_feature    3293..3322
                     /label=5UTR
     misc_feature    3293..3322
                     /label=+30
     primer_bind     complement(3302..3343)
                     /label=QL424-R
     primer_bind     complement(3309..3328)
                     /label=QL571 R
     primer_bind     3323..3342
                     /label=QL752 F
     CDS             join(3329..3881,3998..4314)
                     /codon_start=1
                     /product="E1A"
                     /label=E1A
                     /note="other spliced forms exist with larger introns"
                     /protein_id="DAA00639.1"
                     /translation="MRHIICHGGVITEEMAASLLDQLIEEVLADNLPPPSHFEPPTLHE
                     LYDLDVTAPEDPNEEAVSQIFPDSVMLAVQEGIDLLTFPPAPGSPEPPHLSRQPEQPEQ
                     RALGPVSMPNLVPEVIDLTCHEAGFPPSDDEDEEGEEFVLDYVEHPGHGCRSCHYHRRN
                     TGDPDIMCSLCYMRTCGMFVYSPVSEPEPEPEPEPEPARPTRRPKMAPAILRRPTSPVS
                     RECNSSTDSCDSGPSNTPPEIHPVVPLCPIKPVAVRVGGRRQAVECIEDLLNEPGQPLD
                     LSCKRPRP"
     primer_bind     complement(4294..4335)
                     /label=QL152-R
     misc_recomb     4334..4367
                     /label=LoxP
     protein_bind    4334..4367
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
     misc_feature    4423..5011
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             complement(4894..4905)
                     /label=FactorXa site
     primer_bind     complement(5014..5030)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     LTR             5540..5720
                     /label=HIV-1 5 LTR
     rep_origin      complement(5764..6392)
                     /direction=LEFT
                     /label=ColE1 origin
     rep_origin      complement(5782..6370)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    6322..6327
                     /label=U6 terminator
     misc_feature    6323..6328
                     /label=U6 terminator
     misc_feature    6355..6360
                     /label=U6 terminator
     misc_feature    6356..6361
                     /label=U6 terminator
     CDS             complement(6541..7401)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     CDS             complement(6544..7203)
                     /label=AmpR
     misc_feature    6953..6958
                     /label=U6 terminator
     misc_feature    7360..7365
                     /label=U6 terminator
     promoter        complement(7402..7506)
                     /gene="bla"
                     /label=AmpR promoter
     promoter        complement(7443..7471)
                     /label=Amp prom
ORIGIN
        1 gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg
       61 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt
      121 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc
      181 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac
      241 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat
      301 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
      361 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
      421 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
      481 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
      541 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
      601 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt
      661 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
      721 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg
      781 gcggtaggcg tgtacggtgg gaggtctata taagcagcgc gttttgcctg tactgggtct
      841 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
      901 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
      961 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
     1021 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
     1081 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
     1141 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
     1201 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
     1261 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
     1321 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
     1381 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
     1441 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
     1501 aaagtaagac caccgcacag caagcggccg gccgctgatc ttcagacctg gaggaggaga
     1561 tatgagggac aattggagaa gtgaattata taaatataaa gtagtaaaaa ttgaaccatt
     1621 aggagtagca cccaccaagg caaagagaag agtggtgcag agagaaaaaa gagcagtggg
     1681 aataggagct ttgttccttg ggttcttggg agcagcagga agcactatgg gcgcagcgtc
     1741 aatgacgctg acggtacagg ccagacaatt attgtctggt atagtgcagc agcagaacaa
     1801 tttgctgagg gctattgagg cgcaacagca tctgttgcaa ctcacagtct ggggcatcaa
     1861 gcagctccag gcaagaatcc tggctgtgga aagataccta aaggatcaac agctcctggg
     1921 gatttggggt tgctctggaa aactcatttg caccactgct gtgccttgga atgctagttg
     1981 gagtaataaa tctctggaac agatttggaa tcacacgacc tggatggagt gggacagaga
     2041 aattaacaat tacacaagct taatacactc cttaattgaa gaatcgcaaa accagcaaga
     2101 aaagaatgaa caagaattat tggaattaga taaatgggca agtttgtgga attggtttaa
     2161 cataacaaat tggctgtggt atataaaatt attcataatg atagtaggag gcttggtagg
     2221 tttaagaata gtttttgctg tactttctat agtgaataga gttaggcagg gatattcacc
     2281 attatcgttt cagacccacc tcccaacccc gaggggaccc gacaggcccg aaggaataga
     2341 agaagaaggt ggagagagag acagagacag atccattcga ttagtgaacg gatcggcact
     2401 gcgtgcgcca attctgcaga caaatggcag tattcatcca caattttaaa agaaaagggg
     2461 ggattggggg gtacagtgca ggggaaagaa tagtagacat aatagcaaca gacatacaaa
     2521 ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt tcgggtttat tacagggaca
     2581 gcagagatcc agtttggtta gtaccgggcc cgctctagag atccgacgcg ccatctctag
     2641 gcccgcgccg gccccctcgc acagacttgt gggagaagct cggctactcc cctgccccgg
     2701 ttaatttgca tataatattt cctagtaact atagaggctt aatgtgcgat aaaagacaga
     2761 taatctgttc tttttaatac tagctacatt ttacatgata ggcttggatt tctataagag
     2821 atacaaatac taaattatta ttttaaaaaa cagcacaaaa ggaaactcac cctaactgta
     2881 aagtaattgt gtgttttgag actataaata tcccttggag aaaagccttg ttaacgcgcg
     2941 gtgaccctcg aggtcgacgg tatcgataag ctcgcttcac gagattccag caggtcgagg
     3001 gacctaataa cttcgtatag catacattat acgaagttat attaagggtt ccaagcttaa
     3061 gcggccgccg atgtacgggc cagatatacg cgttctttga aagcagtcga gggggtgcta
     3121 ggtgtgggca gggacgagct ggcgcggcgt cgctgggtgc accgcgacca cgggcagagc
     3181 cacgcggcgg gaggactaca actcccggca caccccgcgc cgccccgcct ctactcccag
     3241 aaggccgcgg ggggtggacc gcctaagagg gcgtgcgctc ccgacatgcc ccgcggcgcg
     3301 ccattaaccg ccagatttga atgaattcat gagacatatt atctgccacg gaggtgttat
     3361 taccgaagaa atggccgcca gtcttttgga ccagctgatc gaagaggtac tggctgataa
     3421 tcttccacct cctagccatt ttgaaccacc tacccttcac gaactgtatg atttagacgt
     3481 gacggccccc gaagatccca acgaggaggc ggtttcgcag atttttcccg actctgtaat
     3541 gttggcggtg caggaaggga ttgacttact cacttttccg ccggcgcccg gttctccgga
     3601 gccgcctcac ctttcccggc agcccgagca gccggagcag agagccttgg gtccggtttc
     3661 tatgccaaac cttgtaccgg aggtgatcga tcttacctgc cacgaggctg gctttccacc
     3721 cagtgacgac gaggatgaag agggtgagga gtttgtgtta gattatgtgg agcaccccgg
     3781 gcacggttgc aggtcttgtc attatcaccg gaggaatacg ggggacccag atattatgtg
     3841 ttcgctttgc tatatgagga cctgtggcat gtttgtctac agtaagtgaa aattatgggc
     3901 agtgggtgat agagtggtgg gtttggtgtg gtaatttttt ttttaatttt tacagttttg
     3961 tggtttaaag aattttgtat tgtgattttt ttaaaaggtc ctgtgtctga acctgagcct
     4021 gagcccgagc cagaaccgga gcctgcaaga cctacccgcc gtcctaaaat ggcgcctgct
     4081 atcctgagac gcccgacatc acctgtgtct agagaatgca atagtagtac ggatagctgt
     4141 gactccggtc cttctaacac acctcctgag atacacccgg tggtcccgct gtgccccatt
     4201 aaaccagttg ccgtgagagt tggtgggcgt cgccaggctg tggaatgtat cgaggacttg
     4261 cttaacgagc ctgggcaacc tttggacttg agctgtaaac gccccaggcc ataagaattc
     4321 gtcgagggac ctaataactt cgtatagcat acattatacg aagttataca tgtttaaggg
     4381 ttccggttcc actaggtaca attcgatatc aagcttatcg ataatcaacc tctggattac
     4441 aaaatttgtg aaagattgac tggtattctt aactatgttg ctccttttac gctatgtgga
     4501 tacgctgctt taatgccttt gtatcatgct attgcttccc gtatggcttt cattttctcc
     4561 tccttgtata aatcctggtt gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa
     4621 cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca ctggttgggg cattgccacc
     4681 acctgtcagc tcctttccgg gactttcgct ttccccctcc ctattgccac ggcggaactc
     4741 atcgccgcct gccttgcccg ctgctggaca ggggctcggc tgttgggcac tgacaattcc
     4801 gtggtgttgt cggggaaatc atcgtccttt ccttggctgc tcgcctgtgt tgccacctgg
     4861 attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc ggaccttcct
     4921 tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc ttcgccttcg ccctcagacg
     4981 agtcggatct ccctttgggc cgcctccccg catcgatacc gtcgacctcg atcgagacct
     5041 agaaaaacat ggagcaatca caagtagcaa tacagcagct accaatgctg attgtgcctg
     5101 gctagaagca caagaggagg aggaggtggg ttttccagtc acacctcagg tacctttaag
     5161 accaatgact tacaaggcag ctgtagatct tagccacttt ttaaaagaaa aggggggact
     5221 ggaagggcta attcactccc aacgaagaca agatatcctt gatctgtgga tctaccacac
     5281 acaaggctac ttccctgatt ggcagaacta cacaccaggg ccagggatca gatatccact
     5341 gacctttgga tggtgctaca agctagtacc agttgagcaa gagaaggtag aagaagccaa
     5401 tgaaggagag aacacccgct tgttacaccc tgtgagcctg catgggatgg atgacccgga
     5461 gagagaagta ttagagtgga ggtttgacag ccgcctagca tttcatcaca tggcccgaga
     5521 gctgcatccg gactgtactg ggtctctctg gttagaccag atctgagcct gggagctctc
     5581 tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt
     5641 agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc
     5701 agtgtggaaa atctctagca gcatgtgagc aaaaggccag caaaaggcca ggaaccgtaa
     5761 aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa
     5821 tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc
     5881 ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc
     5941 cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag
     6001 ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga
     6061 ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc
     6121 gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac
     6181 agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg
     6241 cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca
     6301 aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa
     6361 aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa
     6421 ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt
     6481 aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag
     6541 ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat
     6601 agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc
     6661 cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa
     6721 ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca
     6781 gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa
     6841 cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt
     6901 cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc
     6961 ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact
     7021 catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc
     7081 tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg
     7141 ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct
     7201 catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc
     7261 cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag
     7321 cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac
     7381 acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg
     7441 ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt
     7501 tccgcgcaca tttccccgaa aagtgccacc tgac
//