psiCHECK-2-h1-cluster19

LOCUS       psiCHECK-2-h1-cluster19            6603 bp DNA     circular SYN 02-JUN-2026
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6603)
  AUTHORS   SnapGene License
  TITLE     Direct Submission
  JOURNAL   Exported Sep 15, 2026 from SnapGene 6.0.2
            https://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..6603
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        62..419
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        62..253
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     rep_origin      270..405
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          489..621
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     promoter        666..684
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      688..697
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             694..1629
                     /codon_start=1
                     /product="Renilla luciferase"
                     /label=hRluc
                     /note="human codon-optimized"
                     /translation="MASKVYDPEQRKRMITGPQWWARCKQMNVLDSFINYYDSEKHAEN
                     AVIFLHGNAASSYLWRHVVPHIEPVARCIIPDLIGMGKSGKSGNGSYRLLDHYKYLTAW
                     FELLNLPKKIIFVGHDWGACLAFHYSYEHQDKIKAIVHAESVVDVIESWDEWPDIEEDI
                     ALIKSEEGEKMVLENNFFVETMLPSKIMRKLEPEEFAAYLEPFKEKGEVRRPTLSWPRE
                     IPLVKGGKPDVVQIVRNYNAYLRASDDLPKMFIESDPGFFSNAIVEGAKKFPNTEFVKV
                     KGLHFSQEDAPDEMGKYIKSFVERVLKNEQ"
     misc_feature    1681..2002
                     /label=h1-cluster19
     polyA_signal    2018..2066
                     /note="synthetic polyadenylation signal"
     promoter        2075..2826
                     /label=HSV TK promoter
                     /note="herpes simplex virus thymidine kinase promoter"
     regulatory      2856..2865
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2862..4514
                     /codon_start=1
                     /gene="luc+"
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="enhanced luc+ version of the luciferase gene"
                     /db_xref="GI:42718124"
                     /protein_id="AAS38485.1"
                     /translation="MADAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     RBS             3821..3829
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     polyA_signal    4558..4679
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        4812..4916
                     /gene="bla"
                     /label=AmpR promoter
     CDS             4917..5777
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      5948..6536
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 agatctgcgc agcaccatgg cctgaaataa cctctgaaag aggaacttgg ttaggtacct
       61 tctgaggcgg aaagaaccag ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag
      121 gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accaggtgtg
      181 gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag
      241 caaccatagt cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc
      301 attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctcgg
      361 cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa
      421 agcttgattc ttctgacaca acagtctcga acttaagctg cagaagttgg tcgtgaggca
      481 ctgggcaggt aagtatcaag gttacaagac aggtttaagg agaccaatag aaactgggct
      541 tgtcgagaca gagaagactc ttgcgtttct gataggcacc tattggtctt actgacatcc
      601 actttgcctt tctctccaca ggtgtccact cccagttcaa ttacagctct taaggctaga
      661 gtacttaata cgactcacta taggctagcc accatggctt ccaaggtgta cgaccccgag
      721 caacgcaaac gcatgatcac tgggcctcag tggtgggctc gctgcaagca aatgaacgtg
      781 ctggactcct tcatcaacta ctatgattcc gagaagcacg ccgagaacgc cgtgattttt
      841 ctgcatggta acgctgcctc cagctacctg tggaggcacg tcgtgcctca catcgagccc
      901 gtggctagat gcatcatccc tgatctgatc ggaatgggta agtccggcaa gagcgggaat
      961 ggctcatatc gcctcctgga tcactacaag tacctcaccg cttggttcga gctgctgaac
     1021 cttccaaaga aaatcatctt tgtgggccac gactgggggg cttgtctggc ctttcactac
     1081 tcctacgagc accaagacaa gatcaaggcc atcgtccatg ctgagagtgt cgtggacgtg
     1141 atcgagtcct gggacgagtg gcctgacatc gaggaggata tcgccctgat caagagcgaa
     1201 gagggcgaga aaatggtgct tgagaataac ttcttcgtcg agaccatgct cccaagcaag
     1261 atcatgcgga aactggagcc tgaggagttc gctgcctacc tggagccatt caaggagaag
     1321 ggcgaggtta gacggcctac cctctcctgg cctcgcgaga tccctctcgt taagggaggc
     1381 aagcccgacg tcgtccagat tgtccgcaac tacaacgcct accttcgggc cagcgacgat
     1441 ctgcctaaga tgttcatcga gtccgaccct gggttctttt ccaacgctat tgtcgaggga
     1501 gctaagaagt tccctaacac cgagttcgtg aaggtgaagg gcctccactt cagccaggag
     1561 gacgctccag atgaaatggg taagtacatc aagagcttcg tggagcgcgt gctgaagaac
     1621 gagcagtaat tctaggcgat cgctcgagcc cgggaattcg tttaaaccta gagcggccgc
     1681 tcaagtgaaa taaaccagaa gcagaaagat aaatatcaca tgttctcact catatgtgga
     1741 agctaaaaag tttctctcat ggaggaagag aatagaatga tagttactag aggcttggaa
     1801 tgtggtggtc agggtgggat gaagagaggg tagttaatga gatagataga tagaaggagt
     1861 aagttcttgt gtttgatagc acactaaggt aaccatagtc aagaactgta tattgtgtgt
     1921 tgtatatttc aaaattgcta gaagagggtc aggtgcagtg gctcacacct gtaatccctg
     1981 cactttggga ggccgaggtg gggcggccgc tggccgcaat aaaatatctt tattttcatt
     2041 acatctgtgt gttggttttt tgtgtgagga tctaaatgag tcttcggacc tcgcgggggc
     2101 cgcttaagcg gtggttaggg tttgtctgac gcggggggag ggggaaggaa cgaaacactc
     2161 tcattcggag gcggctcggg gtttggtctt ggtggccacg ggcacgcaga agagcgccgc
     2221 gatcctctta agcacccccc cgccctccgt ggaggcgggg gtttggtcgg cgggtggtaa
     2281 ctggcgggcc gctgactcgg gcgggtcgcg cgccccagag tgtgaccttt tcggtctgct
     2341 cgcagacccc cgggcggcgc cgccgcggcg gcgacgggct cgctgggtcc taggctccat
     2401 ggggaccgta tacgtggaca ggctctggag catccgcacg actgcggtga tattaccgga
     2461 gaccttctgc gggacgagcc gggtcacgcg gctgacgcgg agcgtccgtt gggcgacaaa
     2521 caccaggacg gggcacaggt acactatctt gtcacccgga ggcgcgaggg actgcaggag
     2581 cttcagggag tggcgcagct gcttcatccc cgtggcccgt tgctcgcgtt tgctggcggt
     2641 gtccccggaa gaaatatatt tgcatgtctt tagttctatg atgacacaaa ccccgcccag
     2701 cgtcttgtca ttggcgaatt cgaacacgca gatgcagtcg gggcggcgcg gtcccaggtc
     2761 cacttcgcat attaaggtga cgcgtgtggc ctcgaacacc gagcgaccct gcagcgaccc
     2821 gcttaaaagc ttggcattcc ggtactgttg gtaaagccac catggccgat gctaagaaca
     2881 ttaagaaggg ccctgctccc ttctaccctc tggaggatgg caccgctggc gagcagctgc
     2941 acaaggccat gaagaggtat gccctggtgc ctggcaccat tgccttcacc gatgcccaca
     3001 ttgaggtgga catcacctat gccgagtact tcgagatgtc tgtgcgcctg gccgaggcca
     3061 tgaagaggta cggcctgaac accaaccacc gcatcgtggt gtgctctgag aactctctgc
     3121 agttcttcat gccagtgctg ggcgccctgt tcatcggagt ggccgtggcc cctgctaacg
     3181 acatttacaa cgagcgcgag ctgctgaaca gcatgggcat ttctcagcct accgtggtgt
     3241 tcgtgtctaa gaagggcctg cagaagatcc tgaacgtgca gaagaagctg cctatcatcc
     3301 agaagatcat catcatggac tctaagaccg actaccaggg cttccagagc atgtacacat
     3361 tcgtgacatc tcatctgcct cctggcttca acgagtacga cttcgtgcca gagtctttcg
     3421 acagggacaa aaccattgcc ctgatcatga acagctctgg gtctaccggc ctgcctaagg
     3481 gcgtggccct gcctcatcgc accgcctgtg tgcgcttctc tcacgcccgc gaccctattt
     3541 tcggcaacca gatcatcccc gacaccgcta ttctgagcgt ggtgccattc caccacggct
     3601 tcggcatgtt caccaccctg ggctacctga tttgcggctt tcgggtggtg ctgatgtacc
     3661 gcttcgagga ggagctgttc ctgcgcagcc tgcaagacta caaaattcag tctgccctgc
     3721 tggtgccaac cctgttcagc ttcttcgcta agagcaccct gatcgacaag tacgacctgt
     3781 ctaacctgca cgagattgcc tctggcggcg ccccactgtc taaggaggtg ggcgaagccg
     3841 tggccaagcg ctttcatctg ccaggcatcc gccagggcta cggcctgacc gagacaacca
     3901 gcgccattct gattacccca gagggcgacg acaagcctgg cgccgtgggc aaggtggtgc
     3961 cattcttcga ggccaaggtg gtggacctgg acaccggcaa gaccctggga gtgaaccagc
     4021 gcggcgagct gtgtgtgcgc ggccctatga ttatgtccgg ctacgtgaat aaccctgagg
     4081 ccacaaacgc cctgatcgac aaggacggct ggctgcactc tggcgacatt gcctactggg
     4141 acgaggacga gcacttcttc atcgtggacc gcctgaagtc tctgatcaag tacaagggct
     4201 accaggtggc cccagccgag ctggagtcta tcctgctgca gcaccctaac attttcgacg
     4261 ccggagtggc cggcctgccc gacgacgatg ccggcgagct gcctgccgcc gtcgtcgtgc
     4321 tggaacacgg caagaccatg accgagaagg agatcgtgga ctatgtggcc agccaggtga
     4381 caaccgccaa gaagctgcgc ggcggagtgg tgttcgtgga cgaggtgccc aagggcctga
     4441 ccggcaagct ggacgcccgc aagatccgcg agatcctgat caaggctaag aaaggcggca
     4501 agatcgccgt gtaataattc tagagtcggg gcggccggcc gcttcgagca gacatgataa
     4561 gatacattga tgagtttgga caaaccacaa ctagaatgca gtgaaaaaaa tgctttattt
     4621 gtgaaatttg tgatgctatt gctttatttg taaccattat aagctgcaat aaacaagtta
     4681 acaacaacaa ttgcattcat tttatgtttc aggttcaggg ggaggtgtgg gaggtttttt
     4741 aaagcaagta aaacctctac aaatgtggta aaatcgataa ggatccaggt ggcacttttc
     4801 ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc
     4861 cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga
     4921 gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt
     4981 ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag
     5041 tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag
     5101 aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgta
     5161 ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg
     5221 agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca
     5281 gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag
     5341 gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc
     5401 gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg
     5461 tagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc
     5521 ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg
     5581 cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg
     5641 gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga
     5701 cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac
     5761 tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa
     5821 aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca
     5881 aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag
     5941 gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac
     6001 cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa
     6061 ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc
     6121 accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag
     6181 tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac
     6241 cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc
     6301 gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc
     6361 ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca
     6421 cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc
     6481 tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg
     6541 ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatggctc
     6601 gac
//